Human Cytomegalovirus 감염시 UL47 promoter 활성의 분석과 Escherichia coli에서 발현되는 UL47 단백질의 분석

현종준,김기호,김홍진 중앙대학교 약학연구소 1997 약학 논총 Vol.11 No.-

In order to study of HCMV(human cytomegalovirus) UL47 promoter activity, we have used the transient transfection assay method. We cloned the -20∼-325bp region to be expected as UL47 promoter part into the indicator plasmid which includes the β-glucuronidase gene. In the transient promoter activation experiment, the promoter activity was increased up to 48-fold in the HCMV(AD169 strain) infected cells. This result indicates that the viral infection is necessary for the UL47 promoter activation occurred in only late times. In order to express UL47 protein in Escherichia coli, we have cloned the N-terminal part(+1∼+818) of UL47 open reading frame(ORF) into pET14b vector. After transformed the cloned DNA(pET14b-UL47N) into BL21(DE3)pLysS, we identified the UL47 protein was expressed by IPTG(isopropyl β-D-thiogalactopyranoside) induction.

정신과 입원환자에서의 혈청 콜레스테롤 농도와 공격행동

전태연,도규영,정종현,권용실,김석범 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 혈청 콜레스테롤 농도와 자살 등의 공격행동간의 연관성에 관한 여러 연구가 있었으나 일치된 결과를 보이고 있지 못하다. 이에 저자들은 다양한 진단의 정신과 입원 환자들 중 공격행동을 보인 환자의 콜레스테롤 농도를 정상 한국 성인에서의 혈청 총 콜레스테롤의 성별, 연령별 평균 및 표준편차와 percentile분포에 맞추어 공격행동 및 자살시도와 혈청 콜레스테롤 농도간의 연관성을 알아보기 위하여 본 연구를 시행하였다. 방 법 : 본 연구는 1994년 1월 1일부터 1995년 6월 31일까지 18개월간 카톨릭대학교 의정부성모병원 정신과 병동에 입원하였던 환자 중 콜레스테롤 농도를 얻을 수 있었던 127명을 대상으로 하였다. 대상 환자는 공격행동의 유무에 따라 공격군과 비공격군으로 분류하여 공격군 35명과 비공격군 92명의 자료를 얻었으며, 공격군은 Overt Aggression Scale을 사용하여 평가하였다. 측정된 각 환자의 콜레스테롤 농도는 정상 한국 성인에서의 혈청 총 콜레스테롤의 성별, 연령별 평균 및 표준편차와 percentile분포에 맞추어 4개의 군으로 나눈 후 공격행동 발생비율, 자살시도간의 차이를 알아보았다. 또한 공격행동을 보인 군에서도 각 콜레스테롤군 간의 총 공격 점수를 비교하였다. 결 과 : 1) 각 콜레스테롤군 간의 공격행동 발생비율에는 유의한 차이가 없었다. 2) 공격군에서 각 콜레스테롤군 간의 총 공격 점수는 유의한 차이를 보이지 않았다. 3) 각 콜레스테롤군 간의 자살시도 빈도는 유의한 차이가 없었다. 결 론 : 정신과 입원 환자의 혈청 콜레스테롤 농도를 정상 한국 성인에서의 혈청 총 콜레스테롤의 성별, 연령별 평균 및 표준편차와 percentile분포에 맞추었을 때 혈청 콜레스테롤 농도와 공격행동 및 자살시도에 유의한 연관이 없었다. Objectives : An association between serum cholesterol concentration and violent behavior has been suggested, but has not been consistently demonstrated. This study was conducted in order to evaluate the relation between serum cholesterol concentration and violent behavior in psychiatric inpatients who had been admitted at Uijongbu St. Mary's Hospital, the Catholic University of Korea from January 1994 to June 1995. Methods : We divided the 127 subjects into violent(35 subjects) and non-violent group(95 subjects). According to the percentile distribution of serum total cholesterol in healthy Korean adults, the subjects were classified into four subgroups : Group 1, subjects Whose cholesterol concentrations were below 26% of percentile distribution, Group 2, from 26% to 50%, Group 3, 51% to 75% and Group 4, above 75%. In the four cholesterol subgroups, the authors examined the incidence of violent behaviors and suicidal attempts. Within the violent group, total aggression score of four cholesterol subgroups was compared. Results : 1) Among the four cholesterol subgroups, there were no significant differences in the violent behavior. 2) Within the violent groups, total violent score of the four cholesterol subgroups showed no significant difference. 3) Among the four cholesterol subgroups, there were no significant differences in suicidal attempt. Conclusion : When the serum cholesterol concentrations of psychiatric inpatient applied to the percentile distribution of serum total cholesterol in healthy Korean adult, no association was found between serum cholesterol concentration and violent behavior and suicide attempt.

복합 운동프로그램이 초등학생의 신체구성, 혈중지질 및 체력에 미치는 영향

전종귀,이왕록,박희근,정준현,이영란,신동원,이경숙 충남대학교 체육과학연구소 2006 體育科學硏究誌 Vol.23 No.1

The purpose of the present study was to examine the effects of combined training on the changes of body composition, blood lipid profile and fitness in elementary school students. Thirty subjects participated in the present study. The subjects were divided into two groups either exercise group for 10weeks(15n) or control group for 10weeks(l5n) The results were described as follows; 1) There were significant differences in the body weight and lean body mass after the exercise program between two groups. 2) There were no significant differences in the blood TC, HDL-C, LDL-C, TG concentration after the exercise program between two groups. 3) There were significant differences in the sit-up and flexibility after the exercise group between two groups. In conclusion, it might be thought that the combined training improved the levels of body composition and health-related fitness in elementary subjects.

지역사회 발전을 위한 해양 레저스포츠 관광의 활성화 방안

전종귀,정문현 忠南大學校體育科學硏究所 2003 體育科學硏究誌 Vol.21 No.1

The purpose of this study is to clarify the reason why marine sports and leisures should be added to Boryoung Mud Festival, to find out the probable problems which would be resulted afterwards, and to find out the way to promote the existing festival events. The conclusions completed from many kinds of documents and data concerned are like these; First, to promote Boryung's tourist industry, much more sightseeing facilities that would help people take part in marine sports such as diving, fishing, wind surfing, yachting, jet-skiing, water-skiing should be supplied. And other amusement parks such as marine museums or water parks should be built to attract lots of tourists' attentions. Furthermore, the wharf that could make people conveniently land or board at Boryung must be built preferentially in order to popularize the marine sports & leisures.. Second, a large scale accommodations complex must be secured to win over a great number of tourists. These facilities must be large enough to accommodate more than 1000 people at once. To attract private capital to build these facilities, it is necessary that the city should support financial back up such as tax cut an construction. Third, marine sports & leisures tend to attract new tourists continuously and let them visit the places repeatedly. And these consumers have such characteristics that they would enjoy sports & leisures all the year round. So in order to satisfy their needs, more attractive, more impressive, and still more creative marine sports programs of Boryung's own must be developed, which can be developed for families or associations of like-minded persons. The present Mud Festival of Boryung is held at the place where tourists can not see the natural shore of an estuary. So there are some limitations to make people feel real mud festival and natural costal environment. Fourth, it is necessary that the Local Government Act should relax restrictions on possessory right permission in open waters and should relieve legal standards on leisure sports facilities. Through these actions, those who are engaged in leisure industry would be motivated to give much more wonderful and touching experiences to the tourists who will visit Boryung. Consequently it could be very helpful to develop the whole sports & leisures industry of Boryung.

유산소 운동과 근력 강화 운동이 노인 당뇨병 환자에 미치는 영향

전종귀,정준현 忠南大學校體育科學硏究所 2003 體育科學硏究誌 Vol.21 No.1

The purpose of this study was to investigate the effects of aerobic exercise and muscle strengthen exercise of elderly patients with type-Ⅱ diabetes mellitus. The subject for this study was twenty elderly patients with type-Ⅱ diabetes mellitus who didn't any problems of complications in participating in this study. Twenty elderly patients with type-Ⅱ diabetes mellitus, were randomly selected and divided into three groups, aerobic exercise group(n=7) and muscle strengthen exercise group(n=7) and control group(n=6). The experimental groups have been trained aerobic and muscle strengthen exercise. In the experimental groups both groups practiced exercise for 50min a day, 4times a week during 12weeks, while the control group did not participated related activities. The aerobic exercise was performed by using cycle ergometer. The muscle strengthen exercise was performed by using elastic band at the intensity of thera-band of yellow color for 1-4weeks, and red/blue color for 5-12weeks. All measured data were represented by mean and standard deviation using SPSS. The first statistical techniques for data analysis were univariate analysis of variance to analyze the difference between these group.

여자고등학교 한국무용수의 춤별 운동강도

全鍾貴,金一坤,金顯美 忠南大學校體育科學硏究所 1992 體育科學硏究誌 Vol.10 No.1

The purpose of this study is to compare the heart rate of Korean dancers who have been trained 6 hours a week for three years with that of general girl students who have never been experienced as an athlete and participated exercise programs regularly. We have studied the change of heart rate until the subject could no longer continue the treadmill exercise by the added velocity every 5 minutes. The following conclusions were obtained. 1. Observation of heart rate about regularity exercise intensity. 1) There was no difference of the heart rate between two groups during the first 5 minutes(3 mph) and 10 minutes(4 mph) of exercise. (p〉.05) 2) The difference of exercise intensity between two groups began to exhibited during 15 minutes (5 mph) of exercise. (p〈.001) 3) During the high-intensity exercise for 20 minutes (6 mph) and 25 minutes (7 mph), the difference of heart rate between two groups was low, but there was high in difference. (p〈.05) 4) The difference of heart rate between two groups in all-out condition for 30 minutes (8 mph) of exercise revealed that the motor skill of general group was superior to that of korean group. (p〈.001) 5) The heart rate recovery of general group was faster than that of korean dance following exercise. 2. Observation of exercise intensity in staging korean dance. 1) The exercise intensity sort of dances were Hwa Kwan Mu 50.4%, Sung Mu 55. 3%, Bu Ch'ae Ch'um 60.2%, O Buk Ch'um 66.7%, Kum Mu 68.3% and Mu Dang Ch'um 89%. 2) The maximum exercise intensity was 89% as a Mu Dang Ch'um. 3) The mean exercise intensity provided by general group for three years constantly was 65%.

Sputter로 제작된 BN박막의 물리적 특성

현준원,주종일 단국대학교 1999 論文集 Vol.34 No.-

We have studied annealing properties of Boron Nitride(BN) thin films. BN thin films were successfully grown on sillicon(100) substrate by rf magnetron sputter deposition. The effects of various annealing on BN thin films have been studied. The Fourier transform infrared absorption spectroscopy (FT-IR) of the depositied BN film (7000Å-1000℃-1-1hour) showed a strong absorption 1090 ㎝-1 clearly indication cubic structure. Surface roughness was observed by Atomic Force Microscope (AFM) and it shows that the roughness increase the phase of cBN. Using Dynamic ultra micro hardness tester, hardness of annealing BN thin film (7000Å-750℃-1-1hour) is about 4000 kgf/mm2 and it shows that it has the possibility of application as hard material. Therefore we conclude that the annealing temperature is related with formation of cBN phase.

이온화에너지 처리 아이스크림 및 우유의 단회투여 독성시험

이희준,최현종,한선경,구본철,신명수,함준상,조철훈,이완규 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

유약 영구 전치의 함입성 탈구에 관한 고찰과 치험례

전경현,김용기,김종수 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.2

In the Pediatric Dentalc profession the occurrence rate intrusion of young permanent teeth is rather low, and the treatment method varies from one another. Intrusion of the teeth bring complications to the pulp and periodontal ligament, and thus the possibility of endodontic treatment increases. For the formation of calcific barriers, calcium hydroxide is used in young permanent teeth and this makes the final canal filling possible. In this case report, we will show two cases of young permanent teeth intrusion and the treatment methods to help assess the recognition of treatment for young permanent teeth intrusion.

유치와 영구치에 수복된 레진과 글라스 아이오노머 수복물의 미세누출 양상에 관한 비교연구

전경현,김종수,김용기 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.1

The purpose of this study was to compare the microleakage pattern of glass ionomer with resin restoration in microleakage pattern of primary and permanent teeth. Micreoscopical observation of interface between tooth structure and restoration was also performed. 80 and 8 sound molar teeth were used for the microleakage test and SEM study respectively. Data were analyzed statistically using ANOVA test and/or t-Test. The results of the present study were as follows: 1. According to the result of microleakage pattern between primary and permanent tooth, primary tooth generally showed more microleakage than permanent tooth in all groups(p<.05). 2. In the resin-filled groups, occlusal margin was shown to have more micreliakage than gingival margin(p<.05). Whereas the glass ionomer-filled groups showed no statestically significant differences between them(p>.05). 3. No statistically significant differences in microleakage could be found between two different resin groups(p>.05). while FujiⅡ LC group showed less microleakage than Ketac-Fil group(p>.05). 4. The various type hybrid layer was evident under SEM in resin-filled groups both in primary and permanent teeth with generally thicker layer in primary group. Among glass-ionomer group, FujiⅡ LC group showed more intact adhesion to tooth surface than Ketac-Fil group.