지중내 공동 탐지를 위한 비파괴 검사 기법에 대한 실험적 연구

유정동(Jund Dong?Yoo),양학모(Hak Mo?Yang),장기창(Ki Chang?Jang),강승민(Seung Min?Kang),조소현(So Hyun?Cho),김현기(Hyun-Ki?Kim),Seung Soo?Kim,Da Bin?Jin 대한토목학회 2018 대한토목학회 학술대회 Vol.2018 No.10

Systemic administration of low dosage of tetanus toxin decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus

Bing Chun Yan,In Hye Kim,Joon Ha Park,Ji Hyeon Ahn,Jeong-Hwi Cho,Bai Hui Chen,Jae-Chul Lee,Jung Hoon Choi,Ki-Yeon Yoo,Choong Hyun Lee,Jun Hwi Cho,Jong-Dai Kim,Moo-Ho Won 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.3

In the present study, we investigated the effect of Tetaus toxin (TeT) on cell proliferation and neuroblast differentiation using specific markers: 5-bromo-2-deoxyuridine (BrdU) as an exogenous marker for cell proliferation, Ki-67 as an endogenous marker for cell proliferation and doublecortin (DCX) as a marker for neuroblasts in the mouse hippocampal dentate gyrus (DG) after TeT treatment. Mice were intraperitoneally administered 2.5 and 10 ng/kg TeT and sacrificed 15 days after the treatment. In both the TeT-treated groups, no neuronal death occurred in any layers of the DG using neuronal nuclei (NeuN, a neuron nuclei maker) and Fluoro-Jade B (F-J B, a high-affinity fluorescent marker for the localization of neuronal degeneration). In addition, no significant change in glial activation in both the 2.5 and 10 ng/kg TeT-treated-groups was found by GFAP (a marker for astrocytes) and Iba-1 (a marker for microglia) immunohistochemistry. However, in the 2.5 ng/kg TeT-treated-group, the mean number of BrdU, Ki-67 and DCX immunoreactive cells, respectively, were apparently decreased compared to the control group, and the mean number of each in the 10 ng/kg TeT-treated-group was much more decreased. In addition, processes of DCX-immunoreactive cells, which projected into the molecular layer, were short compared to those in the control group. In brief, our present results show that low dosage (10 ng/kg) TeT treatment apparently decreased cell proliferation and neuroblast differentiation in the mouse hippocampal DG without distinct gliosis as well as any loss of adult neurons.

연마 다듬질 가공면의 표면 미세형상 평가에 관한 연구

조남규,김현국,권기환,한창수,안유민,이성환,박균명 한국공작기계학회 2000 한국공작기계학회 추계학술대회논문집 Vol.2000 No.-

This paper describes the statistical analysis techniques for the surface roughness assessment of polished surfaces. In experiments, the polishing process of the sample surfaces which are manufactured by ball end mill is consist of two steps; the cusp removal process and the surface finishing process. For the cusp removal process, the criterion of cusp removal was established from the power spectrum analysis to assess the change of the cusp removal rate. For the finishing process, the surface was polished by the rotational CBN tool and vibration wood tool. And the surface quality of polished surface was assessed using the functional parameters based on the statistical values of surface profiles. Consequently, the surface finish performance of the polished surface using the vibration wood tool was improved.

흰쥐 시상하부에서 NMDA에 의한 c-fos의 발현

이영기,최기열,이원돈,유영복,이왕재,황덕호,조사선,장가용 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

최대근력부하와 폭발적 근력부하에 의한 신경-근피로와 그 회복과정 비교 분석

유관호,이무연,이승훈,김민섭,육조영,임정일,임완기,김대경 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The result which was gained from this study is as follows. From the conclusion of this study it was confirmed that resistant motion at high level strength brings about rise in density of lactate in blood at the same time decline of muscular power and generates acute fatigue of nerve-muscle accompanied by decline in electrical activity of muscle. On the other hand explosive loading produced fatigue at body center and decline in nervine-muscular activity particularly for men, In case of women explosive load did not produce fatigue in the same level as men and they were also quicker in recovery after exercise. Based on 2 types of loading method used in this study there was clearly difference in the process of restoration from resistant motion. For greater decline in muscular power and electromyogram activity more longer period of recovery was require.

수평배수이론에 대한 비교연구

조기영,유갑용 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

The conventional three-dimensional consolidation theory is accumulated to observe the behaviour of consolidation on soft clay with a vertical drain installed and it is aimed to find the most suitable 3-D theory by carrying out the axisymetric analysis in F.E.M for which CRISP 93 has been used. The results showed as follows : The axisymetric analysis and Onoue's(1988) theory which is a rigorous solution and an approximate equation show the best suitability.

√s 침하예측기법을 이용한 현장침하량 예측

조기영,최관희,유갑용 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

Prediction of consolidation settlement and time must be always erroneous because of soil media, errors associated with the measurement of soil parameters, drawback of consolidation theories and so on. When Oiling on compressible soils, the observational method is a useful means in pratice to evaluate the final consolidation settlement and time. However, the ousted observational procedures include some disadvantages which are difficulty of finding the linearity in the settlement curves, which a personal error seems to be unavoidable and so on. A new observational procedure(√s method) is suggested here to perform the consolidation analysis in field. As the results of applying the √s method with another methods to two field data, the final settlement predicted by the √s method as well as by the Asaoka's method agreed well with the measured value. However, one by the hyperbolic method(Tan, 1991) was overestimated, and there happened many cases not to predicted by the Hoshino's method. In the settlement curve from the √s method, the linearity between 60 and 90% of the average degree of consolidation is showed, and then the possibility of happening a personal error seems to be unusual. The final consolidation time(at 0.95s_(f)) predicted by the √s method agreed well with the measured one, but one by Asaoka and Tan(1996) methods was very much underestimated or overestimated. The big errors by the two methods seem to be the cause unconsidered the effect of step loading.

Cytokinin과 대두(Glycine max)잎단백질의 결합에 대하여

류기중,박창규,정창조 제주대학교 방사능이용연구소 1986 연구보고 Vol.2 No.-

cytokinin과 단백질의 결합을 간단하게 검정하는 방법으로서 전기영동법을 시도하고, 대두의 잎단백질과 cytokinin의 결합여부, cytokinin에 대한 affinity가 있는 단백질의 종류와 상대적 affinity를 조사하였다. 검토된 전기영동법은 cytokinin과 단백질의 결합 뿐만아니라, cytokinin에 대한 상대적 affinity를 동시에 검정할 수 있는 장점을 가지고 있었다. Ammonium sulfate 침전법, Sephadex G-25 chromatography, paper chromatography, 그리고 전기영동으로 대두의 잎단백질 중에 BA와 결합하는 단백질이 있음을 확인할 수 있었다. 전기영동법으로 검정한 결과 BA와 결합하는 것이 3 group이 있고, 이중에서 전기영동 이동도로 보아 분자량이 작은 단백질 분획과 전기영동 이동도 0.4부근의 분획은 BA에 대한 affinity가 비교적 낮은 반면, 이동도 0.0∼0.2의 분획은 affinity가 큰 것으로 생각되었다. A polyacrylamide gel electrophoresis technique was applied to cytokinin-protein binding assay. Binding of soybean leaf proteins to cytokinin and relative affinities of protein fractions to cytokinin were studied. The electrophoresis technique appeared to be very useful for determination of cytokinin-protein binding, for identification of protein species binding to cytokinin, and for comparison of relative affinities of the proteins to cytokinin. The presence of cytokinin-binding proteins in soybean leaves was confirmed from assays with ammonium sulfate precipitation, Sephadex G-25 chromatography, paper chromatography, and electrophoresis. Three groups of cytokinin-binding proteins were identified in the soybean leaf protein extract and two of the three showed low affinity to cytokinin, however, the third one with mobility between 0.0∼0.2, probably high molecular weight protein (s), showed high affinity in the electrophoretic analysis.

일부 도시지역 성인들의 혈중지질치의 참고치 및 저콜레스테롤혈증에 미치는 요인 분석

유기하,이동배,이태용,조영채,김수영 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.1

Data from 3.982 adults participating in a health examination in a university hospital were analysed to determine the reference values of hyperlipidemia and hypolipidemia, and those were compared with American data. Also health related behaviors affecting hypocholesterolemia were investigated. 29.3% of the males diseased subjects, and as were 25.6% of the females in their twenties, 69.5% of the males in their 60s and 78.6% of the females in their 50s. The rate of diseased subjects tended increase with increasing age. The rates of the diseased were 60.1% for males and 55.2% for females. Among cardiovascular risk factors, the proportion of hyperlipidemia was 46.6%, fat liver 42.2%, hypertension 21.8%, glucose intolerance 13.7%, abnormal EKG 11. 0%, obesity 2.9% and anemia 1.7% for male subjects and hyperlipidemia was 31.2%, fat liver 29. 5%, anemia 27.5%, hypertension 21.1%, abnormal EKG 15.7%, obesity 7.3% for females in that order. The rates of cardiovascular risk factors were higher in males than in females except for anemia, abnormal EKG and obesity. The total cholesterol levels of 95 percentile for males and females were 227.0 mgldl, 226.0 mg/dl and the 5 percentile levels for males and females were 134.0 rag/dl, 131.0 mgldl, respectively. The LDL cholesterol level of 95 percentile for males and females were 150.0 mg/dl, 147.0 mgldl, and the 5 percentile were 53.0 mgldl, 51.0 mg/dl, respectively. The HDL cholesterol 95 percentile levels for males and females were 82.0 mg/dl, 83. 0 mg/dl, and those of 5 percentile were 36.0 mg/dl and 39.0 mg/dl, respectively. These data show that the mean levels of total cholesterol, triglyceride, LDL cholesterol were related to increased age in both sexes, and were slightly higher in the females than in the males over all ages, but those of HDL cholesterol decreased. The high cholesterol level group was associated with age, the amount of alcohol consumption, smoking and body mass index. But that was not associated with meat intake and regular exercise. No significant difference were found among the rates of all diseases except for diseases and anemia up to 160 mg/dl of cholesterol level in both sexes. Sex, regular exercise and glucose intolerance were revealed as influencing factors in the low serum cholesterol levels, but disease and abnormal EKG had a lowing effect for the low cholesterol risks. In conclusion, the reference values of Korean serum lipids were 5-25 mg/dl lower than Americans. The cut-off value to diagnosis hypolipidemia for the Korean is recommended as below 140 mg/dl of serum total cholesterol. The hyperlipidemia cut-off value should above 220 mg/dl of serum total cholesterol.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.