Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

林地利用에 따른 溪流水의 水質變化

具瀟瑛,鄭源玉,金弘洙,朴晋源,麻鎬燮 경상대학교 농과대학 부속연습림 2001 演習林硏究報告 Vol.- No.11

Table 2. Evaluation of stream water quality in three stands by Drinking water quality standard <원문참조> 임지이용에 따른 수질변화 및 오염정도를 구명하여 향후 비점오염원 관리를 위한 기초자료를 제공하고자 경남 진주시 정평리에 위치한 3개 임분(소나무, 밤나무Ⅰ, 반나무Ⅱ임분)에서 2000년 7월부터 9월까지 계류수의 수질변화를 분석하여 얻은 결과는 다음과 같다. 1. 세 임분의 계류수 pH는 소나무 임분(pH6.59)>밤나무 Ⅱ임분(ph 6.53)>밤나무 Ⅰ임분(pH 6.47)계류수 순으로 나타났다. 2. 전기전도도는 밤나무 Ⅰ임분>소나무 임분>밤나무 Ⅱ임분 계류수의 순으로 높게 나타났다. 3. 양이온은 세 임분의 계류수 모두 Ca^2+, Na^+, Mg^2+, K^+, NH_^+의 순으로 높게 나타났다. 4. 음이온은 소나무 임분과 밤나무 Ⅱ임분 계류수에서는 SO_4^2, Cl, NO_3의 순으로 높게 나타났고, 밤나무 Ⅰ임분 계류수에서는 NO_3, SO_4^2, Cl 의 순으로 높게 나타나 차이를 보였다. 5. 소나무, 밤나무 Ⅱ임분 계류수는 pH, NH_4+, NO_3, Cl, SO_4^2이 먹는 물 수질기준 범위내에 있었다. 그러나 밤나무 Ⅰ임분 계류수에서 5항목 중 NO_3는 먹는 물 수질기준을 넘어서는 범위에 있어 임지이용에 따른 하류수질의 오염이 우려되었다. 따라서 이러한 상류지역에서 오염원 차단을 위한 수질정화시설 등의 대책이 마련되어야 할 것으로 사료된다. This study was carried out to clarify the change characteristics of stream water quality by land-use of forest from July to September, 2000 in three stands(Pinus densiflora, Castanea crenata(Ⅰ), Castanea crenata(Ⅱ) stand) of Jeongpyeong-ri, Jinju-si, Gyeongnam. The mean pH of rainfall results in acid rain of 5.3. The pH of stream water in three stands was hugh in order of Pinus densiflora (pH 6.59), Castanea crenata(Ⅱ)(pH 6.53) and Castanea crenata(Ⅰ) stand(pH 6.47). The electrical conductivity of stream water was high in order of Castanea crenata(Ⅰ), Pinus densiflora and Castanea crenata(Ⅱ) stand. Cations of three stand in stream water high in order of Ca^2+, Na^+, Mg^2+, K^+ and NH_4^+. But anions of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand were high in order of SO_4^2, Cl and NO_3. In Castanea crenata(Ⅰ) stand cations of stream water were high in order of NO_3, SO_^2 and Cl. The level of pH, NH_4^+, NO_3, Cl and SO_4^2 of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand reached within the level of domestic use standard for drinking water But the level of NO_3 of stream water in Castanea crenata(Ⅰ) stand was higher than that of domestic use standard. Therefore, non-point sources like forest watersheds which are fertilizer application lands should be taken to the appropriate mitigation measures.

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

서울 시내 4년제 남자 대학생의 우울과 흡연량, 흡연기간, 니코틴 의존도와의 관계

박지영,박소영,이미숙,백수진,신의경,예혜련,오승진,장윤정,조혜진,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

Background: With reports on the relation between smoking and depression appearing since 1980, it has become clear that depression is one of the psychodynamics of smoking, though there has been little or no progress made in the study of whether or not there is indeed a relation between depression and nicotine dependence. Purpose: The purpose of the study was to investigate the effects of depression on the amount and period of smoking and nicotine dependence among university man student smokers. Method: In periodical examination between 14 and 21, May, 2003, using 170 university male. The CES-D(Center for Epidemiologic Studies Depression) Scale and the FTQ(Fagerstrom Tolerance Qustionnaire) were utilized to measure the level of depression and nicotine dependency. The amount of smoking was calculated based on the number of cigarette used. Result: Results indicated that depression had something to do with amount of smoking. Nicotine dependence also was related to depression but total value was relatively low. finally the period of smoking had nothing to do with depression. Conclusion: The results of this study revealed that the amount of smoking of university man student smokers was very high. Both the amount of smoking and nicotine dependence were affected by levels of depression. Accordingly psychiatric nursing access such as emotional support is needed to manage depression and decrease smoking.

캐런 헤세의 『모래 폭풍을 지나서』를 통해 본 생명의 공존성 회복의 비전

박소진(Park So-Jin) 문학과 환경학회 2010 문학과 환경 Vol.9 No.1

This paper examines the close connections between nature and human beings, between human beings in a community, and between human beings’ physical condition and their emotional state, through Karen Hesse’s book, Out of Dust (1997). It describes the harsh reality that farmers in Oklahoma, which was caused by the combination of the economic depression and the most destructive ‘natural disaster’ in the history of the USA, the so-called Dust Bowl. The Dust Bowl resulted from human abuse of the farm plains, the removal of the grass, and the destruction of the natural environmental balance in the plains. This novel, written in the form of a diary, portrays how destructive the dust and drought were during that period, and how devastated farmers’ lives were, focusing on one main character, Billy, her parents, and their neighbors. While this book warns against people’s greed for short term profit and shows their blindness concerning the consequent disaster, it also demonstrates what makes people overcome those hardships, and how the main characters sustain their hope for life despite bitter mistakes and conflicts. Hope is found in the comfort derived from the forces of nature and from people's love and forgiveness for each other, even though nature and people are the very elements that cause the misery and scars in their lives. Nature and people are portrayed in contrasting ways. No matter how destructive and threatening nature is, it is a fundamental influence on people’s lives, and no matter how unforgivable and disappointing people (especially family members) are, they may be a source of great support. Suggesting the importance of the interdependence between nature and human beings, and also between human beings, this book describes these connections by drawing parallels between these four aspects: 1) the state of nature, 2) the state of people’s lives, 3) people’s physical and emotional condition, and 4) people’s relationships. This book implies that nature, human beings’ physical and emotional condition, and the health of the community, are closely related to each other. This is related to an ecological perspective, which perceives the relationship between nature and human beings as an organic whole, no longer regarding them as separate entities.

HSS STI-CMP적용을 위한이중 패드의 최적화

서용진,박성우,김철복,정소용,이경진,김기욱,박창준 대불대학교 2002 大佛大學校大學院 硏究論文集 Vol.- No.1

As the device geometry shrinks to the deep submicron region, chemical mechanical polishing(CMP) planarization become a more essential technique of advanced ULSI process. Also, CMP process was required for the global planarization of inter-metal dielectric(IMD), inter-level dielectric(ILD) layers and interconnections with free-defect. Especially, the complete global planarization of IMD, ILD and interconnections can be achieved only with the CMP process. However, as the IMD and ILD layer gets thinner, several problems were found in the CMP process. It does have various problems such as dishing effect, torn oxide defects and nitride residues in oxide. So, it leads to severe circuit failure, which affects yield. In this paper, we studied the characteristics of polishing pad, which can apply STI-CMP process for global planarization of multilevel interconnection structure. Also, we investigated the effects of different sets of polishing pad, such as soft and hard pad. As an experimental result, hard pad showed center-fast type, and soft pad showed edge-fast type. Totally, the defect level has shown little difference, however, the counts of scratch was defected less than 2 on JR111 pad. Through the above result, we can select optimum polishing pad, so we can expect the improvement of throughput and device yield.

Interacelluar reprogramming of expression, glycosylation, and function of plant-derived anti-virus therapeutic monoclonal antibody

Da-Young Park,Kyung-Jin Lee,Yangkang So,Zhe Lu,Seulki Lee,Jung-Hwan Lee,Seung Han Oh,Yeon Soo Han,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1

Plant genetic engineering has led to production of plant-derived mAb (mAbP), which can provides a safe and economically effective alternative to the conventional antibody expression methods. In this study, the expression levels of mAbP SO57 with or without ER-retention peptide extensions signal (Lys-Asp-Glu-Leu; KDEL) in transgenic tobacco plants were analysed in transgenic plant. The expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL regardless of transcription level. mAbP SO57 with KDEL localized surround to the nucleus suggesting that the mAbP with KDEL is localized in ER. The mAbP without KDEL and mAbH had mainly Golgi type glycans, whereas the ER-localized mAbP with KDEL had glycan profile with both oligomannose type (47.6%) and Golgi type (52.4%). The Fc domains of both purified mAbP (with and without KDEL) and human-derived mAb (mAbH) had similar bingding activity to the Fcγ RI receptor (CD64). Both mAbP (with and without KDEL) had a shorter half-life than mAbH. However, both mAbP with and without KDEL was as effective at neutralizing activity of the rabies virus CVS-11 as the mAbH. These results suggest that ER localization of recombinant mAbP by KDEL reprograms glycosylation and enhances production of the functional antivirus therapeutic antibody in the plant.

Static Method를 이용한 DMF, NMF 및 Diethyl Carbonate 포함계의 고액평형 측정

박소진,황태원 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.4

융점 측정 장치로 자체 제작한 glass still을 이용하여 정적인 방법으로 dodecane+cyclohexanol, 1-dodecanol+N, N-dimethyl-formamide(DMF), p-xylene+DMF, 1-dodecanol+N-methylformamide(NMF), NMF+1-octanol, aceticacid+diethyl carbonate 및 hexadecane+diethyl carbonate 계에 대한 고액평형 (SLE)을 측정하였다. 측정된 고액평형 데이터는 NRTL식에 의해 계산된 값과 비교하여 보았으며, diethyl carbonate 융점의 문헌상 오류를 확인하였다. Solid-Liquid Equilibrium(SLE) data were measured by the static method for the systems of dodecane+cyclohexanol, 1-dodecanol+N,N-dimethylformamide(DMF), p-xylene+DMF, 1-dodecanol+N-methylformamide(NMF), NMF+1-octanol, acetic acid+diethyl carbonate and hexadecane+diethyl carbonate with the self made melting point measuring apparatus. The measured SLE data were correlated with the NRTL equation, and we found that the literature value of melting point for diethyl carbonate is not correct.

고액 상평형의 측정과 추산 연구

박소진,박영신,황태원,한규진,오종혁,원동복 忠南大學校 産業技術硏究所 2001 산업기술연구논문집 Vol.16 No.2

정적인 융점측정 방법으로 benzene+n-dodecane, cyclohexane+phenol, phenol+water, water+sulfolane 및 diethylcarbonate+nitrobenzene계에 대한 고액 평형을 측정하였다. 측정된 고액평형 데이터는 문헌값과 modified UNIFAC에 의해 예측된 값과 비교하였으며, 추산값과 실험값은 ±1.61K 범위에서 잘 일치하였다. 본 연구에서 고찰된 diethylcarbonate의 융점은 기존문헌치와 큰 편차를 보여 재검증이 필요하다. Solid-liquid equilibrium(SLE) data were measured for benzene+ n-dodecane, cyclohexane+phenol phenol+water, water+sulfolane, and diethylcarbonate+ nitrobenzene systems by using static melting point measuring method. Measured SLE data were compared with the literature values and also compared with the values predicted by modified UNIFAC(Dortmund) equation. The experimental results are in good agreements with the published and estimated values, and the average deviation between the experimental values and the calculated values is ±1.16K. The measured melting point of diethylcarbonate shows some difference from literature value, and it will be certified through other experimental data for different systems.

IGCC용 고온건식 탈화제의 반응속도 모사에 관한 연구

박소진,이영우,민세홍,나재익,위영호 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

고온건식 탈황공정에서 탈황제의 황화, 재생 반응은 비촉매 기고 반응이다. 이러한 비촉매 기고 반응의 수학적 모사는 실험데이터의 설명, 공정의 설계 그리고 scale-up에 있어서 매우 중요한 정보가 된다. 탈황제의 황화, 재생 반응에는 spray dryer로 제조된 zinc titanate 탈황제를 사용하였고, 변형된 미반응핵 모델식에 적용하여 각 반응의 반응속도를 해석한 결과 탈황제의 황화반응에는 화학반응 저항과 고체 상태의 확산 저항이 회합하여 전체반응속도를 지배하며, 이때 반응속도상수는 367.67cm/min 이었고 고체상태의 확산계수는 29.66cm²/min이었다. 또한 재생반응시에 는 화학반응 저항만이 전체반응의 저항으로 관여하며, 이때의 반응속도상수는 3.45x10e cm/min이었다. In the dry-type high temprature gas desulfurization process, sulfidation and regeneration reactions of desulfrrization sorbents are noncatalytic gas-solid reactions. The mathematical modeling of noncatalytic gas-solid reactions is improtant in order to interpret laboratory data and in design and scale-up. Zinc titanate sorbent to manufacture by spray dryer is used in sulfidation and regeneration reactions of desulfuization sorbents. The result which analyzes reaction rate of sulfidation reaction by applying each reactions to modified unreacted core model is that the global reaction rate is controlled by associating with chemical reaction resistance and solid state diffusion resistance. In this case,reaction rate constant is367.67e cm/min and solid state diffusion coefficient is 29.66e cm²/min Also gloval reaction rate of regeneration reaction is controlled only by chemical reaction resistance. In this case, reaction rate constant is 3.45x10e cm/min.