Exogenous Local Hyperthermia at 41℃ Is Effective to Eliminate Mouse Model of Sporotrichosis, Independent of Neutrophil Extracellular Traps Formation

( Ya-lan Wang ),( Rui-qun Qi ),( Jing Lan ),( Zheng-xiu Li ),( Xing-hua Gao ) 대한피부과학회 2021 Annals of Dermatology Vol.33 No.1

Background: Local hyperthermia is recommended for the treatment of patients with fixed cutaneous sporotrichosis, though the effectiveness and mechanisms of action remain elusive. While neutrophils represent the main inflammatory cells associated with sporotrichosis lesions, the issue of whether hyperthermia is involved with interactions between neutrophils and Sporothrix globosa remains unclear. Objective: To evaluate the effect of local hyperthermia on sporotrichosis and determine whether local hyperthermia involves effects of neutrophils against Sporothrix. Methods: For the in vivo study, mice were infected with yeast cells of S. globosa followed by treatment with local hyperthermia. In vitro, an isolated Sporothrix strain was co-cultured with or without neutrophils and subjected under different temperatures. Immunofluorescence was used to assess the formation of neutrophil extracellular trap (NETs) were formed under these different culture conditions and the number of fungi colony forming units were compared. Results: Hyperthermia was significantly more effective in clearing the lesions in the mouse model, as compared to sham treatment. Neutrophils failed to exert any fungicidal effects against S. globosa in response to hyperthermia. Moreover, NETs were formed after interaction with S. globosa, and the percentage of NETs formed was not significantly different at 41℃ or 37℃. Conclusion: While hyperthermia could serve as an effective therapy for fixed cutaneous sporotrichosis, this ability does not involve the formation of NETs. (Ann Dermatol 33(1) 37∼45, 2021)

HOCl Oxidation-modified CT26 Cell Vaccine Inhibits Colon Tumor Growth in a Mouse Model

Zhou, Rui,Huang, Wen-Jun,Ma, Cong,Zhou, Yan,Yao, Yu-Qin,Wang, Yu-Xi,Gou, Lan-Tu,Yi, Chen,Yang, Jin-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Despite progress in elucidating mechanisms associated with colorectal cancer and improvement of treatment methods, it remains a frequent cause of death worldwide. New and more effective therapies are therefore urgently needed. Recent studies have shown that immunogenicity of whole ovarian tumor cells and subsequent T cell response were potentiated by oxidation modification with hypochlorous acid (HOCl) in vitro and ex vivo. These results prompted us to investigate the protective antitumor response with an HOCl treated CT26 colorectal cancer cell vaccine in an in vivo mouse model. Administration of HOCl modified vaccine triggered robust antitumor immunity to autologous tumor cells in mice and prolonged survival period significantly. In addition, increased necrosis and apoptosis were found in tumor tissue from the oxidation group. Interestingly, ELISPOT assays showed that specific T cell responses were not elicited in response to the immunizing cellular antigen, in contrast to raising sera antibody titer and antibody binding activity shown by ELISA assay and flow cytometry. Further evaluation of the mechanisms underlying HOCl modified vaccine mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results combined with previous studies suggest that HOCl oxidation modified whole cell vaccine has wide applicability as a cancer vaccine because it can target both T cell- and B cell-specific responses. It may thus represent a promising approach for the immunotherapy of colorectal cancer.

Synthesis and Characterization of KTiNbO₅ Nano-particles by Novel Polymerizable Complex Method

Wang, Ning-Ning,Lan, Yun-Xiang,He, Jie,Dong, Rui,Hu, Jin-Song Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

The layered $KTiNbO_5$ was successfully synthesized with titanium(IV) isopropoxide and niobium oxalate by a novel polymerized complex (PC) method. The morphology and structure of the as-prepared sample was characterized by means of High-Resolution Transmission Electron Microscope, powder X-ray diffraction, and Laser Raman Spectroscopy. The spectral response characteristic was recorded by using UV-vis Diffuse Reflectance Spectroscopy. Results show that $KTiNbO_5$ as-prepared by PC method presents an uniform morphology of nano-particles, the mean particle sizes is ca. 28 nm corresponding to the (002), and the crystal structure can be well indexed to the orthorhombic phase. The sample as-prepared by PC method has higher band gap energy than that of the sample prepared by a solid-state reaction method due to the quantum size effect.

Integrated wear prediction model for cylindrical gear with variable hyperbolic circular arc tooth trace under mixed elastohydrodynamic lubrication

Yongqiao Wei,Dong Yang,Rui Guo,Zhongtao Ren,Zeyu Li,Lan Luo 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.8

Cylindrical gear with variable hyperbolic circular arc tooth trace (VH-CATT) is a new type of gear. Sliding wear is the main mode of the surface failure of multiple mechanical parts. Both the lubrication state and contact temperature considerably influence wear characteristics, which may aggravate the transmission performance of gear pairs. Wear, contact temperature, as well as lubrication states are jointly explored. Therefore, an integrated wear prediction model was proposed through taking into account flash contact temperature and surface roughness of VH-CATT cylindrical gears in mixed elastohydrodynamic lubrication. According to the equivalent ellipse contact model of VH-CATT cylindrical gears and tooth surface equation, normal curvature and velocity relations for VH-CATT cylindrical gears were observed, and the normal meshing force was obtained through the consideration of load sharing coefficients and quality grades. Flash contact temperature was estimated by using the literature. This study proposes analytical solutions for investigating how various surface roughness, operation, and geometric parameters affect asperity contact ratio (ACR), asperity contact pressure (ACP), flash contact temperature (FCT), as well as wear depth (WD) related to driving gears. ACR, ACP, FCT, as well as WD initially decrease and then increase from engaging-in to engagingout processes. The minimum occurs at the pitch point. The WD declines as module, cutter radius, and rotational velocity increase while augmenting when surface roughness and torques increase. The maximum and minimum wear depths in driving gears occur at the dedendum and pitch point, respectively. Its overall wear is reduced by 23.16 % compared to the wear of spur gears. The results are valuable for the studies of tooth pitting, wear resistance, and fatigue life improvement for VH-CATT cylindrical gear. These studies can provide verification data and references required for engineering designs and VH-CATT cylindrical gear operations.

Prevalence, Associated Risk Factors, and Phylogenetic Analysis of Toxocara vitulorum Infection in Yaks on the Qinghai Tibetan Plateau, China

Kun Li,Yanfang Lan,Houqiang Luo,Hui Zhang,Dongyu Liu,Lihong Zhang,Rui Gui,Lei Wang,Muhammad Shahzad,Suolang Sizhu,Jiakui Li,Yangzom Chamba 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.5

Exergo-economic analysis of finned tube for waste heat recovery including phase change heat transfer

Shuang-Ying Wu,Jing-Rui Jiu,Lan Xiao,You-Rong Li,Chao Liu,Jin-Liang Xu 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.11

In this paper, an exergo-economic criterion, i.e. the net profit per unit transferred heat load, is established from the perspective of exergy recovery to evaluate the performance of finned tube used in waste heat recovery. Also, the dimensionless exergy change number is introduced to investigate the effect of the flow (mechanical) exergy loss rate on the recovered thermal exergy. Selecting R245fa as a working fluid and exhaust flue gas as a heat source, the effects of the internal Reynolds number Rei, the external Reynolds number Reo, the unit cost of thermal exergy εq, the geometric parameter of finned tube ηoβ and the phase change temperature Tv etc. on the performance of finned tube are discussed in detail. The results show that the higher Tv and ηoβ, and lower Rei may lead to the negligible flow (mechanical) exergy loss rate. There exists an optimal value of Rei where the net profit per unit transferred heat load peaks, while the variations of Reo, εq and Tv cause monotonic change of the net profit per unit transferred heat load. The phase change temperature exerts relatively greater influence on the exergo-economic performance of finned tube in comparison with other parameters. And there exists a critical phase change temperature, where the net profit per unit transferred heat load is equal to zero.

Genotype­specific methylation of HPV in cervical intraepithelial neoplasia

Yaw-Wen Hsu,Rui-Lan Huang,Po-Hsuan Su,Yu-Chih Chen,Hui-Chen Wang,Chi-Chun Liao,Hung-Cheng Lai 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.4

Objective: Hypermethylation of human papillomavirus (HPV) and host genes has beenreported in cervical cancer. However, the degree of methylation of different HPV typesrelative to the severity of the cervical lesions remains controversial. Studies of the degree ofmethylation associated with the host gene and the HPV genome to the severity of cervicallesions are rare. We examined the association of methylation status between host genes andlate gene 1 (L1) regions of HPV16, 18, 52, and 58 in cervical brushings. Methods: Cervical brushings from 147 HPV-infected patients were obtained. The samplescomprised normal (n=28), cervical intraepithelial neoplasia (CIN) 1 (n=45), CIN2 (n=13), andCIN3/carcinoma in situ (n=61). The methylation status of HPV and host genes was measuredusing bisulfite pyrosequencing and quantitative methylation-specific polymerase chainreaction (PCR). Results: The degree of methylation of L1 in HPV16, 18, and 52 was associated with theseverity of the cervical lesion. In HPV52, C-phosphate-G (CpG) sites 6368m, 6405m, and6443m showed significantly higher methylation in lesions ≥CIN3 (p=0.005, 0.003, and0.026, respectively). Methylation of most HPV types except HPV52 (r<−0.1) was positivelycorrelated with the degree of methylation of host genes including PAX1 and SOX1 (0.4≤r≤0.7). Combining HPV methylation with PAX1 methylation improved the clustering for ≥CIN2. Conclusion: Our study showed that the degree of L1 methylation of HPV16, 18, and 52but not 58 is associated with the severity of cervical lesions. The association betweenHPV methylation and host gene methylation suggests different responses of host cellularepigenetic machinery to different HPV genotypes.

Microwave hydrothermal synthesis and temperature sensing behavior of Lu2Ti2O7:Yb3þ/Er3þ nanophosphors

Jinsheng Liao,Qi Wang,Lifang Lan,Jiangfei Guo,Liling Nie,Suijun Liu,He-Rui Wen 한국물리학회 2017 Current Applied Physics Vol.17 No.3

Lu2Ti2O7:Yb3þ/Er3þ (LTO:Yb3þ/Er3þ) nanophosphors codoped with Yb3þ (8e20 at%) and Er3þ ions (0.5 e4 at%) were synthesized by a microwave hydrothermal process. Under the 980 nm excitation, the sample gives a set of upconversion light: very strong red emissions near 661 nm (4F9/2 / 4I15/2), weak green around 523 nm and 545 nm (2H11/2/4I15/2 and 4S3/2/4I15/2, respectively). The optimum doping concentrations of Er3þ and Yb3þ for the highest emission intensity were determined by photoluminescence (PL) analyses. Concentration dependent studies revealed that the optimal composition was realized for the 12 at% Yb3þ and 2.0 at% Er3þ-doping concentration with a strong emission. A possible UC mechanism for LTO:Yb3þ/Er3þ is discussed via the change of the pump power. The temperature dependence of the fluorescence intensity ratios (FIR) for the two green UC emission bands peaked at 523 and 545 nm was studied in the range of 298e573 K under excitation by a 980 nm diode laser and the maximum sensitivity was approximately 0.00313 K『1 at 536 K. This indicates that LTO:Yb3þ/Er3þ nanophosphors are potential candidates for optical temperature sensors with high sensitivity.

African Maternal Origin and Genetic Diversity of Chinese Domestic Donkeys

Lei, Chu-Zhao,Ge, Qing-Lan,Zhang, Hu-Cai,Liu, Ruo-Yu,Zhang, Wei,Jiang, Yong-Qing,Dang, Rui-Hua,Zheng, Hui-Ling,Hou, Wen-Tong,Chen, Hong Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

The origin of domestic donkeys in China has been controversial. To clarify the origin of Chinese domestic donkeys, we investigated the partial mitochondrial D-loop sequences of 126 samples from 12 native breeds. The results revealed two mitochondrial origins, lineage Somali and lineage Nubian of African wild ass detected in Chinese domestic donkeys. Lineage Somali was predominant in Chinese domestic donkey breeds. The pattern of genetic variation in ass mtDNA D-loop sequences indicated that the two lineages Somali and Nubian from China had undergone population expansion events. In a combined analysis of lineages Somali and Nubian between previously published sequences from other countries/regions and sequences of Chinese domestic donkeys, the results indicated that the two lineages of Chinese domestic donkeys were from Africa and supported the African maternal origins of Chinese domestic donkeys. There was no obvious geographical structure in Chinese domestic donkey breeds, but the population showed abundant mtDNA diversity. The spread routes of Chinese domestic donkeys were also discussed.

Synthesis and Characterization of KTiNbO5 Nano-particles by Novel Polymerizable Complex Method

Ning-ning Wang,Yun-xiang Lan,Jie He,Rui Dong,Jin-song Hu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

The layered KTiNbO5 was successfully synthesized with titanium(IV) isopropoxide and niobium oxalate by a novel polymerized complex (PC) method. The morphology and structure of the as-prepared sample was characterized by means of High-Resolution Transmission Electron Microscope, powder X-ray diffraction, and Laser Raman Spectroscopy. The spectral response characteristic was recorded by using UV-vis Diffuse Reflectance Spectroscopy. Results show that KTiNbO5 as-prepared by PC method presents an uniform morphology of nano-particles, the mean particle sizes is ca. 28 nm corresponding to the (002), and the crystal structure can be well indexed to the orthorhombic phase. The sample as-prepared by PC method has higher band gap energy than that of the sample prepared by a solid-state reaction method due to the quantum size effect.