Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

공공도서관 주변 공공시설이 도서관 서비스 인식에 미치는 영향

박형준(Park, Hyung-Jun),전선민(Jun, Sun-Min),정주철(Jung, Ju-Chul) 대한국토·도시계획학회 2018 國土計劃 Vol.53 No.4

This study examined the impact of community facilities on parents` perceptions of visiting public libraries. Previous research on public libraries has focused on the services provided by libraries, including the provision of information and the space for holding programs in the buildings. Urban planners have also insisted that the interaction between primary and convenience functions makes libraries work best. However, previous research has paid little attention to the connection between libraries and community facilities. We investigated whether the proximity of parks and transportation services has an impact on parents` perceptions on visiting libraries with their children. A field survey was conducted at 8 public libraries in Busan Metropolitan City, and the data from 488 respondents were analyzed with the ordered logit model. The results revealed that parents were more likely to visit public libraries near parks. Therefore, librarians, policymakers, and urban planners should consider the location and function of public libraries in relation to community facilities/services during the urban planning and design process to encourage the use of public libraries by parents and their children.

개에서 이중 결찰법을 통한 동맥관 개존증의 완치 예

윤헌영,정순욱,박희명,박철,정만복,김준영,한현정,황민,노병국,박상혁,장하영,박정윤 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.1

A ten months old, female Yorkshire terrier weighing 2.88 kg referred to veterinary teaching hospital of college of veterinary medicine, Konkuk University because of syncope, cough and dyspnea. First hematological and serum chemical test revealed thrombocytopenia, mild anemia, and increase of concentration of ALP (195 U/L). On 57 days later, second hematological and serum chemical test revealed polycythemia, increase of concentration of ALP (211 U/L), and Tchol (387 mg/dl). Right atrium enlargement, main pulmonary artery bulge and cardiomegaly (VHS = 11.5) were observed in radiographic findings. Ultrasonographic images showed both right and left ventricular dilation and turbulent flow between the descending aorta and the main pulmonary artery in color Doppler imaging. ECG showed left ventricular enlargement, SA block, and electrical alternant. Thoracotomy was performed through left fourth intercostal incision under isoflurane anesthesia. Patent ductus arteriosus was double ligated with 1-0 silk. Cough and dyspnea disappeared on 5 days after operation. Turbulent flow was not found in color doppler imaging of ultrasonography on 10 days after operation. Ten months later after the operation, syncope could not exist any more.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

內部熱源을 갖는 直方體의 傳熱特性에 관한 硏究

朴春根,全哲昊,徐海成 동아대학교 공과대학 부설 한국자원개발연구소 1984 硏究報告 Vol.8 No.2

A numerical and experimental study have been performed on the heat transfer in rectangular solid owned heat source. The effects of thermal conductivity k, heat transfer coefficient h, temperature of surrounding fluid To and the rate of heat flow Q were studied numerically by F. E. M. The experiment were studied by given rectangular solid. The results of F. E. M. and experimental are as follows. 1) The F. E. M. to calculate the temperature distribution in rectangular solid showed good agreement with experimental. 2) Thermal conductivity influenced strongly temperature distribution in the solid but heat transfer cofficient influenced only surface. 3) Temperature gradient in the solid was unconcerned by temperature of surrounding fluid and rate of heat flow but temperature in the solid changed at a fixed rate.

장애인 체육 업무 부처이관에 따른 조직체계 구축과 발전 방향

박준동,신동철 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

As the Korea physical education business for the disabled will be transferred to the Ministry of Culture & Tourism this research conclude as follows on the construction of organizational system and developmental course according to agency transfer through literature analysis. First, a joint task force in which the concerned parties participate should be constituted in accordance with a business transfer of physical education for the disabled. Second, an exclusive responsibility agency within the Ministry of Culture & Tourism should be newly organized through the amendments of governmental organization law and national physical education promotion law or the enactment of physical education promotion law for the disabled based by the legal enactment & amendment. Third, an independent organization, is such as tentatively named "Korean Physical Education Association for the Disabled" and "Korean Paralympics Committee", should be founded and fostered as agencies under the umbrella of government. Forth, a prudence in solving problems due to agency transfer must be taken into consideration. Fifth, the special research activities should be strengthened for the disabled' physical education development.

생활체육활동이 장애인의 태도와 만족도에 미치는 영향

박준동,신동철 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This research analysed the vital statistical feature, the daily life physical activities attitude, the life satisfaction , the types of daily life physical activities compared with the disabled' attitude end satisfaction using a stratified cluster sampling method on 214 the orthopedically handicapped persons, the mentally retarded, the visually impaired, the deals, the cerebral lesion retard who are people over sixteen years old, live in Busan, Gyeongsangnamdo, Ulsan, Daegu, Gyeongbuk, and are participating in or interested in the daily life physical activities. The conclusions are as follows: First, there are significant differences among the attitudes and satisfactions of daily life physical activities according to vital statistical feature. Second, there are significant differences among the attitudes on whether the disabled are participated in the daily life physical activities or not. Third, there are significant differences among the life satisfaction and the attitude related physical activities according to the frequency of daily life physical activities participation. Forth, there are significant differences among the concerned requirements according to type of retardation.