Production of bioactive ginsenoside Rg3(S) and compound K using recombinant Lactococcus lactis

Lingli Jiang,Soo Jin Lee,Qiu Ping Yuan,임완택,김선창,NAM SOO HAN 고려인삼학회 2018 Journal of Ginseng Research Vol.42 No.4

Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis. Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors b-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture). Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield. Conclusion: This study demonstrates that the lactic acid bacteria having specific b-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.

Potassium Humate-Derived Nitrogen-Doped Activated Carbons with Narrow Micropore Size Distribution for High-Performance Supercapacitors

Lingli Xie,Litao Kang,Yae Li,Mangwei Cui,Bo Chang,Haiwei Jiang,Lili Gao,Xiaomin Wang,Shan Yun 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.4

Micro-porous activated carbons (ACs) with a narrow pore size distribution of 0.4–0.6 nm and high specific surface areas (1160–1315 m2 · g-1) are prepared from environment-friendly, lowgrade potassium humate (HA-K, carbon resource) and mild activating agent potassium acetate (CH3COOK). Microstructure characterizations indicate that the introduction of activating agent CH3COOK is a key step to achieve high specific surface area and carbonization degree. These ACs contain small amount of oxygen and nitrogen, and show obvious pseudo-capacitance besides double layer capacitance. As a result, the optimized ACs achieve high specific capacitances of 311 F · g-1 and 317 F · g-1 at 0.1 A · g-1 in 2 M KOH and 1 M H2SO4 aqueous electrolytes, respectively. This sample also shows a good charge-discharge cycling stability within 10 000 cycles.

Performance and Design of Shear Connectors in Composite Beams with Parallel Profiled Sheeting at Elevated Temperatures

Ling-Zhu Chen,Gianluca Ranzi,Shou-Chao Jiang,Faham Tahmasebinia,Guo-Qiang Li 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.1

This paper describes an extensive experimental and numerical study conducted to evaluate the thermo-structural response of shear connectors embedded in composite slabs with steel sheeting parallel to the steel beam, with particular focus on opentrapezoidal profiles. For this purpose, eight push-out tests were carried out at different levels of temperature. A threedimensional finite element model was developed in Abaqus and its accuracy was validated against the experimental measurements collected as part of this study. The model was then used to perform a parametric study to gain insight into the structural response at different temperatures. The experimental and numerical results were then used to evaluate the accuracy of available European guidelines for predicting the capacity of shear connectors at elevated temperatures (when embedded in composite slabs with the profiled sheeting parallel to the steel beam). Finally, a new design equation was proposed to calculate the degradation factor defining the resistance of shear connectors for different levels of temperature

Pokemon Inhibits Transforming Growth Factor β-Smad4-Related Cell Proliferation Arrest in Breast Cancer through Specificity Protein 1

Ling Chen,Jing Zhong,Jiang-Hua Liu,Duan-Fang Liao,Ying-Ying Shen,Xiao-Lin Zhong,Xiao Xiao,Wen-Jun Ding,Xiu-Da Peng,Wei Xiong,Xu-Yu Zu 한국유방암학회 2019 Journal of breast cancer Vol.22 No.1

Purpose: Pokemon, also known as ZBTB7A, belongs to the POZ and Krüppel (POK) family of transcription repressors and is implicated in tumor progression as a key proto-oncogene. This present study aimed at determining the mechanism by which Pokemon inhibits transforming growth factor β (TGFβ)-Smad4 pathway-dependent proliferation arrest of breast cancer cells via specificity protein 1 (SP1). Methods: Over-expressing plasmid or small interfering RNA (siRNA) transfection was used to regulate Pokemon levels. The EdU incorporation assay, MTS assay, and clone formation were used to identify the inhibitory effect of Pokemon siRNA on cell proliferation. Quantitative real-time polymerase chain reaction assay confirmed that Pokemon deletion inhibited the expression of proliferation-associated genes. The dual-luciferase reporter assay, electrophoretic mobility shift assay, and co-immunoprecipitation assay were used to analyze binding between Pokemon, Smad4, and SP1. Results: Pokemon deletion induced proliferation arrest of breast cancer cells and inhibited the expression of proliferation-associated genes, especially Smad4. Pokemon bound with SP1 to interdict Smad4 promoter activity. Information on clinical samples was obtained from The Cancer Genome Atlas data, in which the Pokemon mRNA levels showed a negative correlation with Smad4 levels in different subtypes of breast cancer in two independent datasets. Conclusion: We demonstrated that Pokemon binds to SP1 to down-regulate Smad4 expression, thereby promoting proliferation of breast cancer cells. This suggests that Pokemon is a potential TGFβ-signaling participant in breast cancer progression.

High-sensitivity detection for cantharidin by ratiometric fluorescent sensor based on molecularly imprinted nanoparticles of quantum dots

Jiang Ling,Wenqi Zhang,Zijia Cheng,Yanjun Ding 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-

Cantharidin (CTD), an effective component of Chinese herbal medicine with unique efficacy, caused poisoningor death constantly for incorrect use. Therefore, there is an intense need for a reliable, selective,sensitive and low-costing analytical method for monitoring the concentration of CTD in biological samples. In this work, a ratiometric fluorescent sensor (CdTe@MIPs/CDs@NIPs) was successfully constructedfor selective determination of CTD by simply integrated the non-imprinted polymers coated CDs(CDs@NIPs) with cadmium telluride quantum dots coated with molecular imprinted polymers(CdTe@MIPs). CDs@NIPs were directly used as reference signal without addition of CTD templates andthe as-prepared CdTe@MIPs were response signal. With the CTD addition, the fluorescence intensity ofCdTe@MIPs decreased, while the fluorescence of CDs@NIPs remained approximately unchanged, resultinga detection limit as low as 0.15 nM. By spiked human blood samples, the ratiometric sensor was successfullyapplied to CTD detection with good recoveries of 96.12–107.40% and relative standard deviation(RSD) of 2.87–3.96%. Moreover, after centrifuging and re-eluting, the developed sensor could be effectivelyrecycled and reused five times. Significantly, the proposed platform with low-cost and highsensitivityopens a door towards the practical applications of ratiometric sensor and shows immensepotential in pursuing the sensitive, reliable and low-cost determination of poisons in forensic toxicologyanalysis.

Concurrent Engineering Based Collaborative Design Under Network Environment

Jiang Gongliang,Huang Hong-Zhong,Fan Xianfeng,Miao Qiang,Ling Dan The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.10

Concurrent Engineering (CE) is a popular method employed in product development. It treats the whole product design process by the consideration of product quality, cost, rate of progress, and demands of customers. The development of computer and network technologies provides a strong support to the realization of CE in practice. Aiming at the characteristics of CE and network collaborative design, this paper built network collaborative design system frame. Through the analysis of the network collaborative design modes based on CE, this paper provided a novel network collaborative design integration model. This model can integrate the product design information, design process, and knowledge. Intelligent collaboration was considered in the proposed model. The study showed that the proposed model considered main factors such as information, knowledge, and design process in collaborative design. It has potential application in CE fields.

Comparison of leaf transcriptomes of cassava “Xinxuan 048” diploid and autotetraploid plants

Ling Yin,Junjie Qu,Huiwen Zhou,Xiaohong Shang,Hui Fang,Jiang Lu,Huabing Yan 한국유전학회 2018 Genes & Genomics Vol.40 No.9

Polyploidy breeding of cassava has been used to improve cassava traits over the past years. We previously reported in vitro induction of tetraploids in the cassava variety “Xinxuan 048” using colchicine. Significant differences in morphology and anatomy were found between the diploid and tetraploid plants. However, very little is known about the transcriptome difference between them. In this study, morphological and physiological characteristics including leaf thickness, plant height, internode length, chlorophyll content, and photosynthetic capacity were measured. Further, we investigated and validated the difference in gene expression patterns between cassava “Xinxuan 048” tetraploid genotype and its diploid plants using RNA sequencing (RNAseq) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Significant differences in morphology and physiology were observed during tetraploidization. A comparison revealed that tetraploidy induced very limited changes in the leaf transcriptomes of cassava “Xinxuan 048” diploid and autotetraploid plants. However, the differentially expressed genes (DEGs) between 2× and 4× plants, especially those upregulated in 4× plants, were strongly associated with hormonal and stress responses. Large changes in morphology and physiology between the diploid cassava “Xinxuan 048” and its autotetraploid were not associated with large changes in their leaf transcriptomes. Moreover, the differently expressed genes related to the regulation of gibberellin and brassinosteroids potentially explained why the plant height and internode length of 4× plants became shorter. Collectively, our results suggest that 4× cassava is potentially valuable for breeding strains with improved stress resistance.

Construction and Differential Screening of a cDNA Library Specific to Osmotic Stress of Haloxylon ammodendron Seedlings

Jiang, Xiao-Cheng,Guo, Xin-Hong,Pan, Xiao-Ling,Song, Song-Quan Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.5

A subtracted cDNA library specific to osmotic stress of Haloxylon ammodendron (Mey.) Bge seedlings was constructed by suppression subtractive hybridization (SSH) and T/A cloning. SSH was performed between two groups of H. ammodendron seedlings, one was cultivated in Hoagland (H) solution as a driver and the other group was treated with osmotic stress of the Hoagland solution by the addition of 400 mM mannitol (M), as a tester. The library consisted of about 400 recombinant clones, with the average size being of 500 bp, ranging from 300 bp to 1500 bp. Using a PCR-select differential screening kit, 100 recombinant clones were randomly chosen from the subtracted cDNA library and hybridized with forward,reverse subtracted and unsubtracted probes for two rounds. As a result, 21 positive clones specific to osmotic stress were obtained and some of them were verified by Northern blot analysis. The sequencing analysis of 6 positive clones and the following homology comparison to GenBank [blastx] non-redundant databases characterized that two sequences obtained in this experiment may contribute to novel drought-related genes.

Iso-propanol assisted preparation of individualized functional palygorskite fibers and its impact on improving dispersion abilities in polymer nanocomposites

Lingli Ni,Pengyin Zhang,Jing Chen,Jinglong Jiang,Shijie Ding 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.6

Palygorskite (PAL) as a natural one-dimensional nanomaterial has attracted tremendous attention as reinforcement agent in polymer nanocomposites. But its intrinsic existing form of aggregates or bundles and hydrophilic properties, highly requires an eco-efficient and environmentally benign approach for both of the disaggregation and organo-modification of PAL. We report a facile and effective process to achieve individualized organo-modified PAL fibers. It was carried out through surface modification reactions in the form of PAL iso-propanol gels with various alkyl and functional silanes. In contrast to the modifications in highly toxic solvent of toluene, reactions in iso-propanol make it possible to modify the surface of individual PAL fibers to obtain isolated organo-modified PAL fibers. With such a relatively green procedure, even higher amount of organic substituent has been grafted on to the surface of PAL fibers. Consequently, excellent dispersion of modified PAL nanofibers in acrylate polymer coatings was achieved, which exhibits outstanding corrosion protection properties.

A piecewise linear transverse shear transfer model for bolted side-plated beams

Ling-Zhi Li,Chang-Jiu Jiang,Ray Kai-Leung Su,Sai-Huen Lo 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.62 No.4

The performance of bolted side-plated (BSP) beams is affected by the degree of transverse partial interaction, which is a result of the interfacial slip caused by transverse shear transfer between the bolted steel plates and the reinforced concrete beams. However, explicit formulae for the transverse shear transfer profile have yet to be derived. In this paper, a simplified piecewise linear shear transfer model was proposed based on force superposition principle and simplification of shear transfer profiles derived from a previous numerical study. The magnitude of shear transfer was determined by force equilibrium and displacement compatibility condition. A set of design formulae for BSP beams under several basic load cases was also derived. Then the model was verified by test results. A worked example was also provided to illustrate the application of the proposed design formulae. This paper sheds some light on the shear force transfer mechanism of anchor bolts in BSP beams, and offers a practical method to evaluate the influence of transverse partial interaction in strengthening design.