Production of bioactive ginsenoside Rg3(S) and compound K using recombinant Lactococcus lactis

Lingli Jiang,Soo Jin Lee,Qiu Ping Yuan,임완택,김선창,NAM SOO HAN 고려인삼학회 2018 Journal of Ginseng Research Vol.42 No.4

Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis. Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors b-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture). Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield. Conclusion: This study demonstrates that the lactic acid bacteria having specific b-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.

Overexpression and Clinicopathological Contribution of DcR3 in Bladder Urothelial Carcinoma Tissues

Jiang, Yi-Qiang,Zhong, Teng-Fei,Dang, Yi-Wu,Zou, Ling-Song,Yang, Liu,Yang, Xia,Chen, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: To explore the expression of DcR3 protein and its clinicopathological significance in bladder urothelial carcinomas (BUC). Materials and Methods: Immunohistochemistry was performed to detect the expression of DcR3, caspase-3, Bcl-2, VEGF, Ki-67, PCNA and P53 in 166 BUC and 56 normal bladder tissues. Western blotting was used to detect the expression of DcR3 in the supernatants of cultured BUC cells. Results: Overexpression of DcR3 was found in BUC tissues and cell lines, with significant elevation as compared to normal bladder tissues (p<0.0001). Higher DcR3 expression was related to the status of invasion, lymph node metastasis and recurrence. Furthermore, DcR3 expression was negatively correlated with caspase-3 and positively associated with Bcl-2, VEGF, Ki-67 labeling index (LI), PCNA LI and P53 (all p<0.0001), respectively. Conclusions: DcR3 may play a crucial role as an oncogene in tumorigenesis, deterioration and progress of BUC via influencing related pathways of apoptosis, proliferation and angiogenesis. The detection of DcR3 protein in the formalinfixed and paraffin-embedded samples could assist to predict in prognosis of BUC patients.

Improved kinetics of LiNi_1/3Mn_1/3Co_1/3O₂ cathode material through reduced graphene oxide networks

Jiang, Ke-Cheng,Xin, Sen,Lee, Jong-Sook,Kim, Jaekook,Xiao, Xiao-Ling,Guo, Yu-Guo The Royal Society of Chemistry 2012 Physical chemistry chemical physics Vol.14 No.8

<P>An electronically conducting 3D network of reduced graphene oxide (RGO) was introduced into LiNi<SUB>1/3</SUB>Mn<SUB>1/3</SUB>Co<SUB>1/3</SUB>O<SUB>2</SUB> (LNMC) cathode material in a special nano/micro hierarchical structure. The rate test and cycling measurement showed that the hierarchical networks remarkably improve the high rate performance of LNMC electrode for lithium-ion batteries. The effect of RGO conducting networks on kinetic property was investigated by electrochemical impedance spectroscopy (EIS) and potentiostatic intermittent titration (PITT). The EIS results reveal that the RGO network greatly decreases the resistance of lithium batteries, especially the charge transfer resistance which can be attributed to the significantly improved conducting networks. The enhancement of apparent diffusion coefficient by the RGO conducting networks is shown by PITT. The power performance was found to be limited by the electrical conduction in the two-phase region, which can be greatly facilitated by the hierarchical RGO network together with carbon black. The as-obtained LNMC/RGO cathode exhibits an outstanding electrochemical property supporting the design idea of electronically conducting 3D networks for the high-energy and high-power lithium-ion batteries.</P> <P>Graphic Abstract</P><P>A 3D conducting network of reduced graphene oxide nanosheets is introduced into LiNi<SUB>1/3</SUB>Mn<SUB>1/3</SUB>Co<SUB>1/3</SUB>O<SUB>2</SUB> and is found to significantly promote the kinetics for Li storage. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cp23363k'> </P>

Concurrent Engineering Based Collaborative Design Under Network Environment

Jiang Gongliang,Huang Hong-Zhong,Fan Xianfeng,Miao Qiang,Ling Dan The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.10

Concurrent Engineering (CE) is a popular method employed in product development. It treats the whole product design process by the consideration of product quality, cost, rate of progress, and demands of customers. The development of computer and network technologies provides a strong support to the realization of CE in practice. Aiming at the characteristics of CE and network collaborative design, this paper built network collaborative design system frame. Through the analysis of the network collaborative design modes based on CE, this paper provided a novel network collaborative design integration model. This model can integrate the product design information, design process, and knowledge. Intelligent collaboration was considered in the proposed model. The study showed that the proposed model considered main factors such as information, knowledge, and design process in collaborative design. It has potential application in CE fields.

High-sensitivity detection for cantharidin by ratiometric fluorescent sensor based on molecularly imprinted nanoparticles of quantum dots

Jiang Ling,Wenqi Zhang,Zijia Cheng,Yanjun Ding 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-

Cantharidin (CTD), an effective component of Chinese herbal medicine with unique efficacy, caused poisoningor death constantly for incorrect use. Therefore, there is an intense need for a reliable, selective,sensitive and low-costing analytical method for monitoring the concentration of CTD in biological samples. In this work, a ratiometric fluorescent sensor (CdTe@MIPs/CDs@NIPs) was successfully constructedfor selective determination of CTD by simply integrated the non-imprinted polymers coated CDs(CDs@NIPs) with cadmium telluride quantum dots coated with molecular imprinted polymers(CdTe@MIPs). CDs@NIPs were directly used as reference signal without addition of CTD templates andthe as-prepared CdTe@MIPs were response signal. With the CTD addition, the fluorescence intensity ofCdTe@MIPs decreased, while the fluorescence of CDs@NIPs remained approximately unchanged, resultinga detection limit as low as 0.15 nM. By spiked human blood samples, the ratiometric sensor was successfullyapplied to CTD detection with good recoveries of 96.12–107.40% and relative standard deviation(RSD) of 2.87–3.96%. Moreover, after centrifuging and re-eluting, the developed sensor could be effectivelyrecycled and reused five times. Significantly, the proposed platform with low-cost and highsensitivityopens a door towards the practical applications of ratiometric sensor and shows immensepotential in pursuing the sensitive, reliable and low-cost determination of poisons in forensic toxicologyanalysis.

Construction and Differential Screening of a cDNA Library Specific to Osmotic Stress of Haloxylon ammodendron Seedlings

Jiang, Xiao-Cheng,Guo, Xin-Hong,Pan, Xiao-Ling,Song, Song-Quan Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.5

A subtracted cDNA library specific to osmotic stress of Haloxylon ammodendron (Mey.) Bge seedlings was constructed by suppression subtractive hybridization (SSH) and T/A cloning. SSH was performed between two groups of H. ammodendron seedlings, one was cultivated in Hoagland (H) solution as a driver and the other group was treated with osmotic stress of the Hoagland solution by the addition of 400 mM mannitol (M), as a tester. The library consisted of about 400 recombinant clones, with the average size being of 500 bp, ranging from 300 bp to 1500 bp. Using a PCR-select differential screening kit, 100 recombinant clones were randomly chosen from the subtracted cDNA library and hybridized with forward,reverse subtracted and unsubtracted probes for two rounds. As a result, 21 positive clones specific to osmotic stress were obtained and some of them were verified by Northern blot analysis. The sequencing analysis of 6 positive clones and the following homology comparison to GenBank [blastx] non-redundant databases characterized that two sequences obtained in this experiment may contribute to novel drought-related genes.

Pokemon Inhibits Transforming Growth Factor β-Smad4-Related Cell Proliferation Arrest in Breast Cancer through Specificity Protein 1

Ling Chen,Jing Zhong,Jiang-Hua Liu,Duan-Fang Liao,Ying-Ying Shen,Xiao-Lin Zhong,Xiao Xiao,Wen-Jun Ding,Xiu-Da Peng,Wei Xiong,Xu-Yu Zu 한국유방암학회 2019 Journal of breast cancer Vol.22 No.1

Purpose: Pokemon, also known as ZBTB7A, belongs to the POZ and Krüppel (POK) family of transcription repressors and is implicated in tumor progression as a key proto-oncogene. This present study aimed at determining the mechanism by which Pokemon inhibits transforming growth factor β (TGFβ)-Smad4 pathway-dependent proliferation arrest of breast cancer cells via specificity protein 1 (SP1). Methods: Over-expressing plasmid or small interfering RNA (siRNA) transfection was used to regulate Pokemon levels. The EdU incorporation assay, MTS assay, and clone formation were used to identify the inhibitory effect of Pokemon siRNA on cell proliferation. Quantitative real-time polymerase chain reaction assay confirmed that Pokemon deletion inhibited the expression of proliferation-associated genes. The dual-luciferase reporter assay, electrophoretic mobility shift assay, and co-immunoprecipitation assay were used to analyze binding between Pokemon, Smad4, and SP1. Results: Pokemon deletion induced proliferation arrest of breast cancer cells and inhibited the expression of proliferation-associated genes, especially Smad4. Pokemon bound with SP1 to interdict Smad4 promoter activity. Information on clinical samples was obtained from The Cancer Genome Atlas data, in which the Pokemon mRNA levels showed a negative correlation with Smad4 levels in different subtypes of breast cancer in two independent datasets. Conclusion: We demonstrated that Pokemon binds to SP1 to down-regulate Smad4 expression, thereby promoting proliferation of breast cancer cells. This suggests that Pokemon is a potential TGFβ-signaling participant in breast cancer progression.

A piecewise linear transverse shear transfer model for bolted side-plated beams

Ling-Zhi Li,Chang-Jiu Jiang,Ray Kai-Leung Su,Sai-Huen Lo 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.62 No.4

The performance of bolted side-plated (BSP) beams is affected by the degree of transverse partial interaction, which is a result of the interfacial slip caused by transverse shear transfer between the bolted steel plates and the reinforced concrete beams. However, explicit formulae for the transverse shear transfer profile have yet to be derived. In this paper, a simplified piecewise linear shear transfer model was proposed based on force superposition principle and simplification of shear transfer profiles derived from a previous numerical study. The magnitude of shear transfer was determined by force equilibrium and displacement compatibility condition. A set of design formulae for BSP beams under several basic load cases was also derived. Then the model was verified by test results. A worked example was also provided to illustrate the application of the proposed design formulae. This paper sheds some light on the shear force transfer mechanism of anchor bolts in BSP beams, and offers a practical method to evaluate the influence of transverse partial interaction in strengthening design.

Does HBV Infection Increase Risk of Endometrial Carcinoma?

Jiang, Xue-Feng,Tang, Qiong-Lan,Zou, Yuan,Xu, Ling,Zeng, Hua,Chi, Cong,Jiang, Jing-Ru,Zhang, Bin-Zhong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: Connections between chronic inflammation and tumor development and progression are now generally accepted. Recent evidence indicates that hepatitis B is associated with several types of cancer, but whether endometrial carcinoma (EC) is included has not been reported. Methods: We analyzed HBV serum marker status in 398 patients with endometrial cancer, comparing them to 788 control women undergoing health examination. Results: The total prevalence of HBsAg tested positive in cancer group was significantly higher than the control group (12.8% vs 6.0%, P=0.001), while positive HBsAb was significantly lower (41.2% vs 68.5%, P=0.001). Hepatitis B carriers in endometrial cancer group were also more frequent than in the control group (9.3% vs 5.5%, P=0.013). Interestingly, in the endometrial cancer group, 147 cases were HBV serum marker negative, which was also higher than in the control group (36.9% vs 15.6%, P=0.001). Conclusion: There may be a correlation between HBV infection and endometrial carcinoma.

Potassium Humate-Derived Nitrogen-Doped Activated Carbons with Narrow Micropore Size Distribution for High-Performance Supercapacitors

Lingli Xie,Litao Kang,Yae Li,Mangwei Cui,Bo Chang,Haiwei Jiang,Lili Gao,Xiaomin Wang,Shan Yun 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.4

Micro-porous activated carbons (ACs) with a narrow pore size distribution of 0.4–0.6 nm and high specific surface areas (1160–1315 m2 · g-1) are prepared from environment-friendly, lowgrade potassium humate (HA-K, carbon resource) and mild activating agent potassium acetate (CH3COOK). Microstructure characterizations indicate that the introduction of activating agent CH3COOK is a key step to achieve high specific surface area and carbonization degree. These ACs contain small amount of oxygen and nitrogen, and show obvious pseudo-capacitance besides double layer capacitance. As a result, the optimized ACs achieve high specific capacitances of 311 F · g-1 and 317 F · g-1 at 0.1 A · g-1 in 2 M KOH and 1 M H2SO4 aqueous electrolytes, respectively. This sample also shows a good charge-discharge cycling stability within 10 000 cycles.