Cisplatin과 Tobramycin이 白鼠 Corti器에 미치는 영향에 關한 電子 顯微鏡的 硏究 : Ultrastructural Study

김동환,신태균,김무강,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

In order to study the morphological changes of Corti’s organ in the rat treated with cisplatin and tobramycin, thirty-three rats, weighing between 200∼250g, were used, and divided into 4 groups, i.e., 3 rats were used for control, and 30 rats were used for 3 experimental groups. Each experimental group was divided into cisplatin-treated, tobramycin-treated, and both cisplatin and tobramycin treated group with 10 rats. Rats were injected with a single dose of 7.7㎎/㎏ of cisplatin intraperitoneally and 50㎎/㎏/day of tobramycin for 2 days intramuscularly, respectively or both. Treated rats were sacrificed on the 3rd and 5th day after the first injection. Results were as follows; 1. Cisplatin-treated group: Outer hair cells of Corti’s organ were degenerated with the formation of vacuoles and appearance of lysosomes, however, inner hair cells showed mild vacuolization. 2. Tobramycin-treated group: NO pathological changes were found in Corti’s organ. 3. Combined cisplatin and tobramycin treated group: Outer and inner hair cells showed vacuolization, appearance of lysosomes, myelin figures and multivesicular body. There were also multivesicular body in the inner phalangeal cell and myelin figure in the Reissner’s membrance. 4. Degeneration of Corti’s organ in the combined treated group was more sever than those of the single cisplatin-treated or tobramycin-treated group. 5. NO pathological differences of Corti’s organ in duration of postinjection were manifested in any groups. It can be concluded that cisplatin induces toxicity to inner ear and cisplatin enhance toxicity more in continued treated with tobramycin.

병재배 폐톱밥을 이용한 양송이 복토재료 개발에 관한 연구

김홍규,이희덕,김용균,한규홍,문창식,김홍기 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

애느타리 병재배후 탈병한 톱밥을 3월 중순 퇴적하여 부숙시킨 결과 퇴적기간에 따른 폐톱밥의 부숙정도는 퇴적 30일이후에 가장 컸으며 부숙 소요일수는 최소 48일이었다. 퇴적 기간에 따른 폐톱밥의 pH 및 총 질소 함량은 부숙이 진전됨에 따라 증가하는 경향이었고 총 탄소 및 C/N율은 시간이 경과함에 따라 감소하는 경향이었다. 식양토에 부숙이 완료된 톱밥을 부피 비율로 10, 30, 50%씩 첨가하여 복토 재료로 사용한 결과 30% 첨가시 식양토 단용 처리보다 A. bisporus 균사 생장이 촉진되고 초발이 소요일수가 5일 빠르며, 수량에 있어서도 28% 증수되었다. After bottle culture of Pleurotus ostreatus, sawdust was taken out from the bottle and accumulated in the middle of March, and then composted. As the result, Y value was decreased rapidly 30 days after composting, and it was decreased slowly after 30 days. It is considered that 118 days is required for composting, however, it is possible to use for casing material after at least 48 days composting. The pH and total nitrogen content of sawdust based on composting period had tendency to increase as composting was processed. Total carbon and C/N rate had tendency to decrease as time went on. Based on the rate of 10, 30 and 50%, each sawdust was added to clay loam used as casing material for culturing A. bisporrus. Among various treatments, the mycelial growth of A. bisporus was more favorable in the treatment of 30% sawdust than in the single treatment of clay loam. Based on the date necessary for primodium formation of A. bisporus, the primodium formation in the treatment of 30% sawdust was reduced to about 5 days as compared with that of any other treatments When 30% sawdust was added to clay loam used as casing material for culturing A. bisporus, the yield of its fruiting body was increased to 28%.

가토에서 일측 요관 부분 및 완전폐색시 신장과 요관의 조직학적 변화

김상현,김용웅,육승모,한동석,노안식,임재성,김홍식,나용길,설종구 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

This study was designed to clarify histologic changes in the experimental rabbit kidney and ureter after unilateral partial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, the partial obstruction followed by 4 weeks, the complete obstruction followed by 2 weeks, and the complete obstruction followed by 4 weeks. The normal control group was consisted of 5 adult white rabbits. The kidneys and ureters for light microscopy were fixed in 10% neutral formalin, embedded in paraffin and stained with hematoxylin and eosin. Abnormal histologic fingings showed in ipsilateral kidney and ureter of all subgroups(such as dilatation of uriniferous tubule, focal necrosis and desquamation of epithelium, interstitial fibrosis, glomerular congestion, widening of Bowman's space, dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis and interstitial inflammation). These findings were most severe in the group of the complete-obstructed ureter for 4 weeks. No significant changes showed in contralateral ureter of all subgroups. The degree of obstruction was severe and period of obstruction was long, the histological changes in ipsilateral side were severe. Despite of changes in ipsilateral side no abnormal findings showed in contralateral side. We suggest that long term experimental investigation of histologic changes in ipsilateral side and compensatory changes in contralateral side have to be studied.

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

가토에 있어서 편측 요관 폐색시 환측, 반대측 요관에 대한 변화 : 조직학적 소견을 중심으로

설종구,김용웅,육승모,노안식,김홍식,김상현,이충식 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

This study was designed to clarify histological changes in the experimental rabbit ureter after unilateral prartial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, partial obstruction followed by 4 weeks, complete obstruction followed by 2 weeks, complete obstruction followed by 4 weeks. The normal control group consisted of 5 adult white rabbits. The H & E stain. light microscopy were used. Abnormal histological fingings were showed in ipsilateral ureter of all subgroups; dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis, interstitial inflammation. These findings were most severe in the complete-obstructed ureter for 4 weeks. There were no significant changes in contralateral ureter of all subgroups. Despite of progressive histological changes in ipsilateral ureter no abnormal findings were showed in contralateral ureter. We suggest that long term experimental investigation for histological changes in ipsilateral ureter and compensatory changes in contralateral ureter should be done.

濟州島方言의 "낭" 語考

金洪植 제주대학교 1982 논문집 Vol.14 No.1

1. Among the words meaning "tree" in the dialects of Jeju-Do are "namu"/namu/, "nang"/na ŋ/, and nam/nam, etc.. But "nang" and "nam" alone have the originality and the diacronic explanantion of the dialect of Jeju-Do, and are used to the present respectively. Moreover, according to the frequency with which, and the lexical environment in which these words are used, the first "nang" is superior to the latter "nam". 2. The word, "nang" is to be analized into /na/plus /- ŋ/, of which /na/ is a basic root and /-ŋ /, a suffix. 3. The word, "nam" is composed of /na/ plus consonant /-m/. /Na/ is a root and /-m/ is a noun-making suffix. This ending very often occurs in the Altaic language, too. 4. "Nang" and "nam" have the diacronic significance linguistically. Both the words can go back to the written language of the 15th century or prior to that century. The word "nang" of the two is assumed to be more archaic. 5. The word, "nang" in its stem analysis is divided into /na/ + /ŋ/, where /ŋ/ is a suffix. This word is made in the way of combining /R+ŋ/. The word-making method of combining /R+ŋ/ is extremely common the Korean language. Especially, the /-ŋ/ ending very often occurs in the dialects of Jeju-Do and provides us with much materials and various variants.

리놀레산 함유 고형 모델시스템의 산화에 미치는 토코페롤 및 베타 카로틴의 영향

김명,이숙희,최홍식 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

리놀레산 혼합물 (리놀레산 64.6%, 올레산 27.4%, 기타 8.0%)에 토코페롤 isomer 및 β-카로틴을 조합한 모델시스템에서는 정제 잣 지방질에 비해 비교적 낮은 온도인 35℃에서도 급속한 산화가 일어났으며 α-토코페롤은 항산화제로 작용하나 첨가농도가 0.05% 보다 높을수록 오히려 산화촉진 효과를 나타내어 본 실험에서도 0.05% 첨가가 산화 안정성을 유지하는 적정농도라고 추정되었다. γ-토코페롤은 항산화작용을 나타내었고 이 때는 α-토코페롤과는 달리 농도에 다른 항산화력의 차이는 나타나지 않았다. 또한 동일 농도에서 α-토코페롤에 비해 γ-토코페롤의 항산화효과가 크며, 동량 혼합시에는 다소 유도기가 연장되었으며 토코페롤의 감소 경향은 α-, γ-순이었다. β-카로틴은 자동산화 초기에 항산화제로 작용하였으나 γ-토코페롤이 함께 존재할 경우에는 산화를 촉진하는 것으로 나타났으며 β-카로틴의 분해는 지방질의 산화가 진행됨에 따라 급격히 일어났다. 따라서 지방질(유리지방산 상태)의 산화안정성에는 β-카로틴 보다 토코페롤의 영향이 크다고 볼 수 있었다. Effects of tocopherols and β-carotene on the oxidation of the solid model system of a free fatty acid mixture (64.5% of linoleic acid ; 26.4% of oleic acid ; 5.0% of palmitic acid) with tocopherols and β-carotene were studied. α-tocopherol revealed an antioxidant activity at the concentration below 0.05%, however, it showed a prooxidant activity when the concentration was higher than 0.05%. The antioxidant activity of γ-tocopherol was not affected by the concentrations in the range of 0.01∼0.10% in the model and γ-tocopherol showed higher antioxidant activity than that of α-tocopherol. It seemed that α-tocopherol was unstable compared to γ-tocopherol during oxidation. β-carotene showed a weak antioxidative activity at the initial stage of this system while β-carotene showed a prooxidant activity in the presence of tocopherol. β-carotene was highly susceptible to autoxidative degradation during oxidation.

民宅三要의 간잡이 방법론에 대한 연구

金鴻植 명지대학교 공학기술연구소 1991 공학기술연구소 논문집 Vol.6 No.-

The contemts "Min Tag Sam Yo"(民宅三要) different from the out of "Tag bo yo jun" (宅普要典), the Chinese concopt of the direction, and the common saying which put the facade of the house south and the main gate east. Eventhough it's methodology had been Spreaded for building House, during the late choseon dynasty Chinese methodlgy was used mainly for con struction Houses by affect of Chinese publicing Culatue.

생활체육교실 참여 동기와 만족도에 관한 연구

김홍식,류창옥 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

The goal of this study is to find necessity of life sports class managements and effective usage method with the survey of motivation and satisfaction for life time sports participants. The data quoted in this paper are collected from questionaries survey was made on male 120 and female 604 participants of life time sports in J city. The results of this study are as follows. 1. The motivation of participation was the first self health management (96.8%), the second, usage of leisure time(94.6%) and the last low attendance fee(75.1%). 2. These research reveal that the main reason why they engage in a life sports class was to self-satisfaction and solve stress. Therefore, the motivation of life sports class participation focus on promote self-health condition than to solve the stress from ordinary life. 3. The satisfactory degree for life sports class reveal that the first, program affinity(77.3%), the second, lecture level(68.4%) and the last, facility(68.4%). In conclusion, life sports classes have a great effect on all aspects of human life and play an important role in solving stress.