Department of Bioengineering

Jian?He Xu 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

Life history responses of the cabbage beetle Colaphellus bowringi to temperature change

Jian‐Jun Tang,Hai-Min HE,Ting GENG,Shu FU,Fang-Sen XUE 한국곤충학회 2016 Entomological Research Vol.46 No.6

Temperature is considered one of the most important mediators of phenotypic plasticity in ectotherms. Here, we investigated life history traits of the cabbage beetle, Colaphellus bowringi Baly (Coleoptera: Chrysomelidae), at a wide range of temperatures (16, 19, 22, 24, 26 and 28°C). The larval and pupal times were significantly decreased with increasing rearing temperature and growth rate was positively correlated with temperature. However, the relationship between body size and rearing temperature in C. bowringi did not follow the temperature–size rule; both males and females reached the highest body weight at 19°C. Females were significantly larger than males at all temperatures. Male pupae lost significantly more weight at metamorphosis compared to females. However, diapausing males gained significantly higher weight after feeding compared to diapausing females at higher temperatures of 22, 24, 26 and 28°C. Body weight tended to decrease with increasing rearing temperature, whereas sexual size dimorphism (SSD) tended to increase with increasing rearing temperature; thus, Rensch's rule is upheld. The degree to which SSD changed with temperature varied with different development stages. SSD was lowest in pupae, highest in newly emerged adults and intermediate in diapausing adults.

DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9

Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.

Roles of Immunohistochemical Staining in Diagnosing Pulmonary Squamous Cell Carcinoma

Yan, Yue,Zhang, Ya-Xiong,Fang, Wen-Feng,Kang, Shi-Yang,Zhan, Jian-Hua,Chen, Nan,Hong, Shao-Dong,Liang, Wen-Hua,Tang, Yan-Na,He, Da-Cheng,Wu, Xuan,Zhang, Li Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Differentiating morphologic features based on hematoxylin-eosin (HE) staining is the most common method to classify pathological subtypes of non-small-cell lung cancer (NSCLC). However, its accuracy and inter-observer reproducibility in pathological diagnosis of poorly differentiated NSCLC remained to be improved. Materials and Methods: We attempted to explore the role of immunohistochemistry (IHC) staining in diagnosing pulmonary squamous cell carcinoma (SQCC) with poorly differentiated features by HE staining or with elevated serum adenocarcinoma-specific tumor markers (AD-TMs). We also compared the difference of epidermal growth factor receptor (EGFR) mutation rate between patients with confirmed SQCC and those with revised pathological subtype. Logistic regression analyses were used to test the association between different factors and diagnostic accuracy. Results: A total of 132 patients who met the eligible criteria and had adequate specimens for IHC confirmation were included. Pathological revised cases in poor differentiated subgroup, biopsy samples and high-level AD-TMs cases were more than those with high/moderate differentiation, surgical specimens and normal-level AD-TMs. Moreover, biopsy sample was a significant factor decreasing diagnostic accuracy of pathological subtype (OR, 4.037; 95% CI 1.446-11.267, p=0.008). Additionally, EGFR mutation rate was higher in patients with pathological diagnostic changes than those with confirmed SQCC (16.7% vs 4.4%, p=0.157). Conclusions: Diagnosis based on HE staining only might cause pathological misinterpretation in NSCLC patients with poor differentiation or high-level AD-TMs, especially those with biopsy samples. HE staining and IHC should be combined as pathological diagnostic standard. The occurrence of EGFR mutations in pulmonary SQCC might be overestimated.

A Directive Programmable Audio Switcher Intelligent Control System

Jian He 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.1

The Audio switcher is high performance intelligent matrix switch equipment which is specially designed for the broadcasting of the audio signal. It is widely used in speech engineering, audio-visual teaching, and command and control center, multi-media conference room and so on and used for switching audio signal. Most of the existing audio switcher support serial control protocol and it can be controlled by the third party software. In this paper, a directive programmable audio switcher intelligent control system was realized. Any kind of audio switcher which supports serial communication protocol can be controlled by this system without any software changing. The only thing to be needed is to change the programmable command list.

Enhanced bone regeneration using poly(trimethylene carbonate)/ vancomycin hydrochloride porous microsphere scaffolds in presence of the silane coupling agent modified hydroxyapatite nanoparticles

Jian He,Xulin Hu,Luyao Xing,Dongliang Chen,Lijun Peng,Gaofeng Liang,Chengdong Xiong,Xiangchun Zhang,Lifang Zhang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

Tissue-engineered scaffolds acted as active natural extracellular matrices that allowed for cellattachment, migration, proliferation, and differentiation. To enhance the drug loading, degradationcontrol, and mechanical features of tissue-engineered scaffolds used in bone regeneration applications,we synthesized novel poly(trimethylene carbonate) (PTMC)/modified HA nanoparticles (KHA NPs,modified by silane coupling agent)/vancomycin hydrochloride (VH) porous microspheres scaffold. Thefabricated PTMC/KHA/VH scaffold has unique surface corrosion degradation, excellent surface properties,and high cytocompatibility. The novel PTMC/KHA/VH scaffold was characterized by 42% porosity,100 mmpore size, and 208.2 MPa compressive modulus. After the treatment of a rat femur defect for 4, 8, and 12weeks, the surface corrosion biodegraded of the PTMC/KHA/VH scaffold remarkably decreasedinflammation response and promoted bone regeneration, suggesting its use as a bioactive structurein bone tissue engineering.

Photocatalytic removal of methyl orange in an aqueous solution by a WO3/TiO2 composite film

Jian He,Qi Zhou Cai,Qiang Luo,Dan Qing Zhang,Teng Teng Tang,You Fang Jiang 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.2

WO3/TiO2 composite film was prepared by microarc oxidation technique and characterized by SEM, XRD,UV-vis spectra and Zeta-potential. The photocatalytic activity of WO3/TiO2 composite film was evaluated by examining the degradation of methyl orange. The influence of solution pH and inorganic anions on removal ratio of methyl orange was investigated. Removal ratio of methyl orange decreased with an increase of pH value in acidic solution, while it increased with the pH value in alkaline solution. The influence of added anions on the removal ratio is divided into two aspects. Addition of Cl− and SO42− resulted in a decrease in photocatalytic removal ratio of methyl orange, while it was facilitated by PO43−, HCO3− and NO3−.

PROXIMAL POINTS METHODS FOR GENERALIZED IMPLICIT VARIATIONAL-LIKE INCLUSIONS IN BANACH SPACES

He, Xin-Feng,Lou, Jian,He, Zhen The Youngnam Mathematical Society 2012 East Asian mathematical journal Vol.28 No.1

In this paper, we study generalized implicit variational-like inclusions and $J^{\eta}$-proximal operator equations in Banach spaces. It is established that generalized implicit variational-like inclusions in real Banach spaces are equivalent to fixed point problems. We also establish relationship between generalized implicit variational-like inclusions and $J^{\eta}$-proximal operator equations. This equivalence is used to suggest a iterative algorithm for solving $J^{\eta}$-proximal operator equations.

A Continuous Abnormal Speech Detection Method Based on Time Domain features Weighted

He Jun,Ji-chen Yang,Qing-hua Zhang,Guo-xi Sun,Jian-bing Xiong 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12

In this brief, a novel pathological continuous speech detection method based on time domain features weighted. First, different optimal threshold for time domain features, including zero crossing ratio, short-time energy and autocorrelation, are obtained from training speech data. Second, a difference evaluation technique is proposed, and with it, the difference of the same time domain feature selected from testing speech data and training speech data were obtained. Finally, to distinguish a given speech well, a novel weighting method based on difference evaluation for each kinds of time domain is employed, respectively. Experiments were conducted on the pathological speech database to prove the power and effectiveness of the proposed method. Results obtained shown that this method outperforms other early proposed time domain feature method, creating a more reliable technique for pathological continuous speech detection.

High alcohol-soluble MoOx gel for interfacial layer in organic solar cells

Jian Xiong,Zhen He,Shiping Zhan,Bingchu Yang,Xiaowen Zhang,Ping Cai,Cong Xu,Xiaogang Xue,Jian Zhang 한국물리학회 2017 Current Applied Physics Vol.17 No.8

Water-free solvent soluble, low-temperature processed metal oxides are important for preparing efficient and stable electronic devices, as well as the convenience in simplifying the device production process. Here we reported a facile approach with the features of low-temperature and solution-based process for the formation of a MoOx (s-MoOx) film as interface layer in polymer solar cells (PSCs). The absorbability, elementary composition, electronic property and surface microstructure of the s-MoOx are investigated in detail by ultravioletevisible spectrophotometer (UVevis), X-ray photoelectron spectrometry (XPS), ultraviolet photo-electron spectrometer (UPS) and atomic force microscopy (AFM). These investigations confirmed that such MoOx xerogel has high solubility in the organic alcohol solvents, such as ethanol and methanol. Meanwhile, this s-MoOx can be applied as the interfacial layer in organic solar cells via a low-temperature treatment (about 100 C) due to its proper physical properties, and a power conversion efficiency (PCE) over 3% was achieved. In addition, the devices with s-MoOx shows excellent air-stability, and the PCE efficiency can maintain about 84% of its initial value after 100 h exposure in air, which is dramatically enhanced comparing with the common devices with PEDOT:PSS layer.