Composition and Antioxidant Activity of Water-Soluble Polysaccharides from Tuber indicum

Qiang Luo,Jie Zhang,Liang Yan,Yuanlin Tang,Xiang Ding,Zhirong Yang,Qun Sun 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.12

Crude water-soluble Chinese truffle Tuber indicum polysaccharide (TIP) was extracted from the fruiting bodies with water and then successively purified by DEAE–cellulose 52 and Sephadex G-100 column chromatography,yielding two major polysaccharide fractions: TIP1-1 and TIP2-1. High-performance gel permeation chromatography analysis showed that the average molecular sizes of TIP1-1 and TIP2-1 were approximately 1.75×10⁴Da and 5.73×10³ Da, respectively. Monosaccharide component analysis by gas chromatography indicated that TIP1-1 was composed of mannose,glucose, galactose, and rhamannose in the respective molar ratio of 3.93:1.24:0.75:1.26 and that TIP2-1 contained mannose,glucose, and arabinose in the respective molar ratio of 5.27:1.44:0.43. The antioxidant activity analyses revealed that TIP1-1and TIP2-1 possessed considerable antioxidant activity. Compared with TIP1-1, which has a higher molecular weight and contains no uronic acid, TIP2-1 exhibited a protective effect on PC12 cells injured by H₂O₂and a higher scavenging activity against free radicals. The relative effects of the lower molecular size, the presence of uronic acid, and the antioxidant activity of TIP2-1 appear to be significant. Accordingly, the Chinese truffle T. indicum might serve as an effective antioxidative healthcare food and source of natural antioxidants.

Induction of Apoptosis in Human Leukemic Cell Lines by Diallyl Disulfide via Modulation of EGFR/ERK/PKM2 Signaling Pathways

Luo, Nian,Zhao, Lv-Cui,Shi, Qing-Qiang,Feng, Zi-Qiang,Chen, Di-Long,Li, Jing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Diallyl disulfide (DADS) may exert potent anticancer action both in vitro and in vivo. Although its effects on cancer are significant, the underlying mechanisms remain unknown. In this study, we sought to elucidate possible links between DADS and pyruvate kinase (PKM2). Materials and Methods: $KG1{\alpha}$, a leukemia cell line highly expressing PKM2 was used with a cell counting kit (CCK)-8 and flow cytometry (FCM) to investigate the effects of DADS. Relationships between PKM2 and DADS associated with phosphorylation of EGFR, ERK1/2 and MEK, were assessed by western blot analysis. Results: In $KG1{\alpha}$ cells highly expressing PKM2, we found that DADS could affect proliferation, apoptosis and EGFR/ERK/PKM2 signaling pathways, abrogating EGF-induced nuclear accumulation of PKM2. Conclusions: These results suggested that DADS suppressed the proliferation of $KG1{\alpha}$ cells, providing evidence that its proapoptotic effects are mediated through the inhibition of EGFR/ERK/PKM2 signaling pathways.

Effect of Argon Gas Pressure on Residual Stress, Microstructure Evolution and Electrical Resistivity of Beryllium Films

Bing-Chi Luo,Kai Li,Ji-Qiang Zhang,Jiang-Shan Luo,Wei-Dong Wu,Yong-Jian Tang 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.4

The residual stress in beryllium films fabricated on K9 substrates by using magnetron sputtering deposition is measured by using a curvature method and is theoretically estimated by using the Nix and Clemens (NC) model. The experimental results indicate that the 1.3-μm-thick film is always in a tensile state for pressure variations in the range from 0.4 to 1.2 Pa. When the sputtering gas pressure is increased, the average stress increases at first, after which it decreases by a remarkable amount. The observed descending trend of the tensile stress when the sputtering gas pressure is beyond 0.6 Pa is mainly attributed to the grain size in the film being larger than that in the film when the pressure is below 0.6 Pa. The maximal residual stress of 552 MPa at a sputtering gas pressure of 0.6 Pa is close to the tensile strength (550 MPa) of the corresponding beryllium bulk material and is about 8 times smaller than that calculated by using the N-C model. In addition, the surface morphologies of the as-fabricated films reveal fibrous grains while the cross-sectional morphologies are characterized by a coarsening of columnar grains. The measured electric resistivity of each film strongly depends on its porosity and the sizes of its grains.

Effect of Trichostatin A on Anti HepG2 Liver Carcinoma Cells: Inhibition of HDAC Activity and Activation of Wnt/β-Catenin Signaling

Shi, Qing-Qiang,Zuo, Guo-Wei,Feng, Zi-Qiang,Zhao, Lv-Cui,Luo, Lian,You, Zhi-Mei,Li, Dang-Yang,Xia, Jing,Li, Jing,Chen, Di-Long Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Purpose: To investigate the effect of deacetylase inhibitory trichostatin A (TSA) on anti HepG2 liver carcinoma cells and explore the underlying mechanisms. Materials and Methods: HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72h were examined for cell growth inhibition using CCK8, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining, and cell morphology changes under an inverted microscope. Expression of ${\beta}$-catenin, HDAC1, HDAC3, H3K9, CyclinD1 and Bax proteins was tested by Western blotting. Gene expression for ${\beta}$-catenin, HDAC1and HDAC3 was tested by q-PCR. ${\beta}$-catenin and H3K9 proteins were also tested by immunofluorescence. Activity of Renilla luciferase (pTCF/LEF-luc) was assessed using the Luciferase Reporter Assay system reagent. The activity of total HDACs was detected with a HDACs colorimetric kit. Results: Exposure to TSA caused significant dose-and time-dependent inhibition of HepG2 cell proliferation (p<0.05) and resulted in increased cell percentages in G0/G1 and G2/M phases and decrease in the S phase. The apoptotic index in the control group was $6.22{\pm}0.25%$, which increased to $7.17{\pm}0.20%$ and $18.1{\pm}0.42%$ in the treatment group. Exposure to 250 and 500nmol/L TSA also caused cell morphology changes with numerous floating cells. Expression of ${\beta}$-catenin, H3K9and Bax proteins was significantly increased, expression levels of CyclinD1, HDAC1, HDAC3 were decreased. Expression of ${\beta}$-catenin at the genetic level was significantly increased, with no significant difference in HDAC1and HDAC3 genes. In the cytoplasm, expression of ${\beta}$-catenin fluorescence protein was not obvious changed and in the nucleus, small amounts of green fluorescence were observed. H3K9 fluorescence protein were increased. Expression levels of the transcription factor TCF werealso increased in HepG2 cells following induction by TSA, whikle the activity of total HDACs was decreased. Conclusions: TSA inhibits HDAC activity, promotes histone acetylation, and activates Wnt/${\beta}$-catenin signaling to inhibit proliferation of HepG2 cell, arrest cell cycling and induce apoptosis.

CORROSION BEHAVIOR OF NI-BASE ALLOYS IN SUPERCRITICAL WATER

QIANG ZHANG,RUI TANG,CONG LI,XIN LUO,CHONGSHENG LONG,KAIJU YIN 한국원자력학회 2009 Nuclear Engineering and Technology Vol.41 No.1

Corrosion of nickel-base alloys (Hastelloy C-276, Inconel 625, and Inconel X-750) in 500 °C, 25 MPa supercritical water (with 10 wppb oxygen) was investigated to evaluate the suitability of these alloys for use in supercritical water reactors. Oxide scales formed on the samples were characterized by gravimetry, scanning electron microscopy/energy dispersive spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The results indicate that, during the 1000 h exposure, a dense spinel oxide layer, mainly consisting of a fine Cr-rich inner layer (NiCr2O4) underneath a coarse Fe-rich outer layer (NiFe2O4), developed on each alloy. Besides general corrosion, nodular corrosion occurred on alloy 625 possibly resulting from local attack of ” clusters in the matrix. The mass gains for all alloys were small, while alloy X-750 exhibited the highest oxidation rate, probably due to the absence of Mo.

FEW WALLED CARBON NANOTUBE PRODUCTION IN LARGE-SCALE BY NANO-AGGLOMERATE FLUIDIZED-BED PROCESS

QIANG ZHANG,HAO YU,YI LIU,WEIZHONG QIAN,YAO WANG,GUOHUA LUO,FEI WEI 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2008 NANO Vol.3 No.1

Few walled carbon nanotubes (FWCNTs) have been successfully synthesized using a nano-agglomerate fluidized-bed process. FWCNTs can be obtained by fluidization of Fe(Co/Ni)/Mo/MgO catalysts at a high temperature with methane cracking in a nano-agglomerate fluidized-bed reactor. The products were mainly 2 to 5 walled CNTs with an outer diameter of 1–7 nm in high purity, as revealed by Raman spectrometry, SEM, and HRTEM analysis. Two keys were crucial for this process. The first key was to get the small size of activity catalyst particles which was realized by Mo addition in catalyst. The graphitization of FWCNTs strongly depended on the composition of catalyst. Fe/Mo/MgO catalyst showed the highest activity and the FWCNT product with the best graphitization. Another key for this process was that the particles must be kept in fluidized state during FWCNT formation. Detailed process information was reported in this article, which showed a potential way for the large scale production of FWCNTs, thereby the urgent need for FWCNTs in high performance will be overcome.

Research on Remote Sensing Image Fusion Algorithm Based on Compressed Sensing

Qiang Yang,Hua Jun Wang,Xuegang Luo 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.5

The traditional image fusion algorithm completed the fusion based on all pixel information. The time and space requirements are higher. The improved fusion algorithm used the theory of compressed sensing (CS) for the processing of remote sensing image fusion. Firstly, the source images using wavelet transform for sparse representation, then, the improved fusion algorithm used the observation matrix for image dimension measurement, and completed the image fusion in CS domain. Finally, the algorithm used the improved OMP algorithm to reconstruct the fused image. The improved fusion algorithm is only applied with a few measurement data of the compressed sensing, and overcomed the shortcomings of traditional pixel level fusion, the fusion algorithm achieved good experimental data.

Facile Preparation of Mechanical Reinforced and Biocompatible Silk Gels

Qiang Zhang,Guocong Han,Chen Lu,Qiusheng Wang,Xiufang Li,Zuwei Luo,Renchuan You,Shuqin Yan 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4

Nontoxic and controllable way to fabricate silk fibroin (SF) gel with high mechanical properties is of critical important to biomaterial in tissue engineering. Electrochemically triggered electrophoretic migration and electric charge of the silk molecules both contributed to SF microspheres and electronic gel (e-gel) formation by sol-gel transition. In this study, a novel silk pH e-gel with higher mechanical property was prepared by combining low-voltage electric fields with isoelectric point (pI) adjustment. This green process was mild and friendly without chemical crosslinker. Compressive modulus of the silk pH e-gel was up to 70 MPa that was significantly higher than that of SF gelation spontaneously. Furthermore, analysis of molecule conformation of the silk pH-e-gel demonstrated that most of random coil structures transformed into α-helix and a little β-sheet structures during this process. The silk pH e-gel was loaded with rhodamine B and showed an obvious sustainable release profile. Accumulation releasing amounts was approximately 60 % at day 9. Cytocompatibility of the silk pH-e-gel was evaluated by epithelial cell. The results showed that the gels could support the cell growth and proliferation in vitro. Finally, gel biodegradation was assessed by protease XIV. After biodegradation for 28 days, remaining weight of the gel was about 20.23±2.59 wt%, indicating its good biodegradability. This novel process was established successfully by combining low voltage field with pH-control, which provided an alternative material for regenerative medicine.

The Prevalence and Genotype of Human Papillomavirus from Patients with Genital Warts in Eastern Guangdong Province

Luo, Zhao-Yun,Chen, Qiang,Yang, Hui,Lin, Min,Chen, Chan-Yu,Yang, Chun,Yang, Li-Ye Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: Low-risk human papillomavirus (LR-HPV) infection is the main cause of genital warts. LRHPV genotypes 6 and 11 are associated with genital warts, but there have only been a few published studies about the genotype-specific prevalence of HPV in genital warts in China. The objective of our study was to assess the prevalence of HPV genotypes for clinical cases involving both men and women and to evaluate the potential benefit of a quadrivalent (genotypes 6, 11, 16, and 18) HPV vaccine in eastern Guangdong province of China. Materials and Methods: A total of 696 eligible patients with genital warts were enrolled during the period Aug 2009 through Oct 2014. Specimens were collected from genital warts, the HPV GenoArray test was used for HPV detection and genotyping, which could detect 21 HPV genotypes, including genotypes 6, 11, 16, and 18. Results: Among the 696 cases, 675 samples were successfully genotyped. The median age of patients was 32.1 years (range, 16-67 years). The most prevalent genotypes were HPV-6 (285/675, 42.2%), HPV-11 (265/675, 39.3%), HPV-52 (52/675, 7.7%), HPV-16 (51/675, 7.56%), HPV-81 (50/675, 7.40%) and HPV-58 (37/675, 5.48%). Low-risk genotypes predominated, with a prevalence of 96.59%. The cumulative prevalence of genotypes 6 and 11 was 78.7% (531/675), the cumulative prevalence of genotypes 16 and 18 was 11.6% (78/675), and the cumulative prevalence of genotypes 6, 11, 16, and 18 was 82.5% (557/675). Conclusions: Our results provide strong evidence that, in eastern Guangdong, different from Western countries, the most prevalent low risk HPV genotypes in patients with genital warts are 6, 11 and 81. The quadrivalent HPV vaccine could prevent 82.5% of genital warts in eastern Guangdong.

Anti-metastasis Activity of Black Rice Anthocyanins Against Breast Cancer: Analyses Using an ErbB2 Positive Breast Cancer Cell Line and Tumoral Xenograft Model

Luo, Li-Ping,Han, Bin,Yu, Xiao-Ping,Chen, Xiang-Yan,Zhou, Jie,Chen, Wei,Zhu, Yan-Feng,Peng, Xiao-Li,Zou, Qiang,Li, Sui-Yan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Increasing evidence from animal, epidemiological and clinical investigations suggest that dietary anthocyanins have potential to prevent chronic diseases, including cancers. It is also noteworthy that human epidermal growth factor receptor 2 (ErbB2) protein overexpression or ErbB2 gene amplification has been included as an indicator for metastasis and higher risk of recurrence for breast cancer. Materials and Methods: The present experiments investigated the anti-metastasis effects of black rice anthocyanins (BRACs) on ErbB2 positive breast cancer cells in vivo and in vitro. Results: Oral administration of BRACs (150 mg/kg/day) reduced transplanted tumor growth, inhibited pulmonary metastasis, and decreased lung tumor nodules in BALB/c nude mice bearing ErbB2 positive breast cancer cell MDA-MB-453 xenografts. The capacity for migration, adhesion, motility and invasion was also inhibited by BRACs in MDA-MB-453 cells in a concentration dependent manner, accompanied by decreased activity of a transfer promoting factor, urokinase-type plasminogen activator (u-PA). Conclusions: Together, our results indicated that BRACs possess anti-metastasis potential against ErbB2 positive human breast cancer cells in vivo and in vitro through inhibition of metastasis promoting molecules.