http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
일차배양된 설치류 호흡기 상피세포로부터의 점액소 분비에 대한 수종 약물의 영향
이충재,석정호,이재흔,허강민,박지선,배소현,노삼길,박상철 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2
1. PKC activator인 PMA는 일차배양 HTSE세포로부터의 뮤신분비를 0.1μM 농도에서 30%, 1μM 농도에서 80% 가량 증가시켰다. 2. 식물 유래 성분으로, flavonoid의 일종인 TFR은 일치배양 HTSE 세포로부터의 뮤신분비를 10μM 농도에서 50%, 100μM 농도에서 80% 가량 증가시켰다. 3. 양이온성 폴리펩티드인 PLL 및 PLA는 일차 배양 HTSE 세포로부터의 뮤신분비를 0.01 - 10μM 농도에서 용량의존적으로 감소시켰다. 4. 결론적으로, 본 연구에서 얻어진 결과들은 새로운 거담제 및 점액용해제나 단백분해 효소제가 아닌 호흡기 류신의 생성/분비를 조절해 줄 수 있는 신개념의 약물을 개발함에 있어 극히 일부분이나마 단서를 제공하고 있다고 하루 수 있을 것이다. In the present study, we tried to investigate whether phorbol myristate acetate(PMA), trihydroxymethoxy-flavanone rutinoside(TFR) and cationic polypeptides significantly affect mucin release(secretion) from cultured hamster tracheal surface epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hr and chased for 30 min in the presence of each agent to assess the effect on 3H-mucin release. The results were as follows : (1) Both PMA and TFR significantly increased mucin release from cultured HTSE cells ; (2) Cationic polypeptides including po1y-L-lysine(PLL, mw 7,500) and poly-L-arginine(PLA, mw 10,800) significantly inhibited mucin release from cultured HTSE cells, in a dose-dependent manner. This finding suggests us that PMA and TFR be further studied for the possible use as mild expectorants and cationic polypeptides might function as a regulator for hyper-secretion of mucus, both by direct acting on airway mucin-secreting cells, during the treatment of chronic airway diseases.
서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)
김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1
In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.
골격근 근형질세망의 ATPase활성도에 대한 Vanillylnonanamide의 영향
박경섭,홍장희,류영수,성지연,허강민,임종호,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2
To investigate the effect of vanillylnonanamide(VN) on the ATPase activity of the sarcoplasmic reticulum(SR) of the skeletal muscle, we prepared the SR vesicles from the back muscle of the rabbit, and measured ATPase activity. The results as follows: Ca-ATPase activity was about 50% in the total ATPase activity of skeletal muscular SR. In the reaction mixture with calcium, 100μM VN increased ATPase activity to 20%, and 100 nM and 10 μM thapsigargin(THP) inhibited the ATPase activity to 50% and 60%, respectively. And 100 μM VN plus 100 nM or 10 μM THP more inhibited ATPase activity than THP alone did it. However, without calcium, 100μM VN did not affect ATPase activity, and 10 μM THP inhibited it to 41%, but VN plus THP inhibited it to 31%. The above results show that VN in the reaction mixture with or without calcium has the different action to ATPase activity when it is used alone or together with Ca-ATPase inhibitor THP. This suggests that VN might increase or decrease the skeletal SR Ca-ATPase activity through affecting the lipid membrane around the Ca-ATPase.
C6 glia 세포에서 유도성 Nitric Oxide Synthase 유전자 발현조절에 관한 연구
배진영,허강민,배소현,박지선,이충재,이재흔,석정호 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1
To investigate transcriptional regulation of iNOS gene by LPS and cytokines, the production of NO, expression of iNOS mRNA and protein, binding activity of nuclear factor-kappa B(NF-kB), and promoter activity of iNOS gene were examined in rat C6 glial cells. LPS, interferon-gamma(IFN-γ), and tumor necrosis factor-alpha (TNF-α) stimulated the production of NO, which was increased synergistically by co-treatment. By the treatment of LPS, iNOS mRNA expression was initiated at 1 h, markedly increased by 3 h, and decreased gradually afterward. iNOS mRNA expression was markedly enhanced by mixture of LPS, IFN-γ and TNF-α. iNOS protein synthesis was increased by the treatment of mixture LPS and cytokine mixture. Treatment of LPS stimulated NF-kB activation, and the activation reached to the maximum level at 30 min, and the treatment of mixture of LPS and cytokines increased the activation. To determine the effect of NF-kB binding activity on iNOS promoter activation, CAT assay was performed. iNOS promoter activity was increased by the treatment with LPS for 5.5 h, and further increased by the combined treatment with LPS and cytokines. These results suggest that NF-kB activation by LPS and cytokines may play a significant role in the induction of the iNOS gene.
Ji Heun Jeong,Jong Hoon An,Hui Yang,Do-Kyung Kim,Nam-Seob Lee,Young-Gil Jeong,Chun Soo Na,Dae Seung Na,Mi-Sook Dong,Seung Yun Han 대한해부학회 2017 Anatomy & Cell Biology Vol.50 No.3
Post-menopausal osteoporosis (PMO) is a major global human health concern. Owing to the need for therapeutic drugs without side effects, natural extracts containing various polyphenolic compounds that may exert estrogenic effects have been studied in depth. Rhus verniciflua Stokes (RVS), which has been used as a traditional herbal medicine for centuries in Korea, was recently revealed to exert estrogenic effects attributable to its bioactive ingredients sulfuretin and butein, which have strong estrogen receptor–binding affinities. In this study, the protective potential of RVS in PMO was evaluated by using an experimental animal model of PMO, which was established by ovariectomy (OVX) of female Sprague Dawley rats. The oral administration of RVS at 20 mg/kg or 100 mg/kg for 8 weeks markedly protected against OVX-induced atrophy of the uterine tube and reversed the elevation in the ratio of serum receptor activator of nuclear factor-kB ligand to osteoprotegerin, which is a marker of disease severity. In addition, RVS inhibited OVX-induced tibia bone loss, activated osteogenic activity, and suppressed osteoclastic activity in the tibial epiphyseal plate, a region of bone remodeling. Collectively, these factors indicated that the oral intake of RVS might be beneficial for the prevention of PMO.
Water Extract of Mixed Mushroom Mycelia is Protective against Experimental Focal Cerebral Ischemia
Ji Heun Jeong,Shin Hye Kim,Ah La Choi,Ji Hyun Moon,Ki Duck Kim,Nam Seob Lee,Young Gil Jeong,Do Kyung Kim,Seung Yun Han 대한체질인류학회 2021 대한체질인류학회 학술대회 연제 초록 Vol.64 No.-
Although the individual consumption of medicinal mushrooms, including Phellinus linteus (PL), Ganoderma lucidum (GL), and Inonotus obliquus (IO) is known to be neuroprotective, the associated mechanisms underlying their therapeutic synergism on focal cerebral ischemia (fCI) have yet to be elucidated. This study aimed to demonstrate the neuroprotective effects of mixed mushroom mycelia (MMM) against experimental fCI. The water-, ethanolic-, and ethyl acetate-fractions of the MMM (PL, GL, and IO) grown in a barley medium using solid-state fermentation techniques were prepared, and their protective effects against glutamate-induced excitotoxicity were compared in PC-12 cells. After identification of the water fraction of MMM (wMMM) as the most suitable form, having the lowest toxicity and highest efficacy, further analyses for evaluating the anti-apoptotic effects of wMMM, including Hoechst 33258-based nuclear staining, fluorescence-activated cell sorting, and reactive oxygen species (ROS) detection assays, were performed. Rats were subjected to a 90-min middle cerebral artery occlusion and reperfusion, after which a wMMM treatment resulted in significant, dose-dependent improvements across a number of parameters. Furthermore, measurements of intracellular ROS and levels of antioxidant enzymes revealed a wMMM-mediated ROS attenuation and antioxidant enzyme upregulation. We suggest that wMMM is neuroprotective against fCI through its anti-apoptotic and anti-oxidative effects.
Ji Heun Jeong,Hyun Young Park,Shin Hye Kim,Nam Seob Lee,Young Gi Jeong,Do Kyung Kim,Seung Yun Han 대한체질인류학회 2021 대한체질인류학회 학술대회 연제 초록 Vol.64 No.-
Alzheimer's disease (AD) is the main cause of dementia characterized by a progressive impairment in cognitive function and brain pathologies including amyloid-β plaques and the adjacent neuroinflammation. Considering the role of reactive oxygen species (ROS)-triggered oxidative damages as a key upstream of AD pathology, dietary polyphenols, which are natural ROS-scavenger, and the polyphenol-rich medicinal mushrooms are receiving scientific attentions. Here, the water fraction of mixed mushroom mycelium, i.e., Phellinus linteus, Ganoderma lucidum, and Inonotus obliquus, grown on solid barley medium was tested for the potential therapeutic effect against AD in vitro and in vivo. The thioflavin S fluorescence assay demonstrated that Zinc-induced increment of intracellular amyloid-beta (Aβ) aggregates were significantly inhibited in the PC-12 cells, a rat neuron-like cell line, treated with mixed mushroom mycelium (MMM). The results from 2ʹ,7ʹ-Dichlorofluorescin Diacetate, terminal deoxynucleotidyl transferase dUTP nick end labeling, and fluorescence-activated cell sorting assay indicated that Zinc-induced ROS accumulation, cell death, and apoptosis were all attenuated in the cells pre-incubated with MMM. Next, using transgenic mice that co-express five familial AD mutations (5XFAD mice), we tested its anti-AD effect in vivo. Behavioral and histologic studies demonstrated that cognitive decline, Aβ plaques accumulation, and the adjacent neuroinflammation were all attenuated in 5XFAD mice subjected with 8-weeks-of oral administration of MMM. Taken together, these results suggest that regular consumption of MMM can attenuate the cognitive decline and the key AD pathologies such as Aβ plaques accumuation and neuroinflammation, via inhibition of intraneuronal Aβ aggregation and the related neurotoxicity.