Characterization of the European type of maternal lineage evident in extant Jeju native pigs

Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.

지구성 운동이 자연발생 고혈압 흰쥐의 혈압에 미치는 영향

이규승,전병화,김일곤,전종귀 忠南大學校體育科學硏究所 1996 體育科學硏究誌 Vol.14 No.1

The purpose of this study is to investigate the effects of indurance exercise on BP of SHR Experimental animals were SHR(180) and SD(90) and they were divided into three groups ; a experimental group1( practice group), a experiment group2(non practice group), and a comparative group (normal group). The experimental group 1 was practicing on a treadmill at 15m/min exercise intensity for 30 minutes a day four times per week for eight weeks. While raising experimental animals, We measured BP for each group once a week, and took the weight of the heart after killing a rat. and then we examined the concentration of ?? and ??, compared, and analyzed them. we have reached the following results. 1. As time passed, BP in the experimental group 1 was almost equal to BP eight weeks ago. BP in the experimental group 2 continued to rise and then showed typical hypertension. 2. The three groups were inclined to increase the weight of their hearts as time passed. However, the experimental group 2 presented the weight of hearts increasing compared with the experimental group1 after eight weeks. 3. In the experimental group 1, and experimental group 2 groups, ?? concentration was but ?? concentration increased as time passed. However, the experimental group1 showed fewer changes than the experimental group 2. 4. The weight of all three groups were inclined to increase as time passed, but it didn't present any noteworthy differences. According to the analysed result of the above experiment, endurannsce exercise becomes an important factor in preventing the raising of BP of SHR, and the hypertrophy of hearts. Key Words : Spontaneously Hypertension Rats, Blood Pressure, Endurance Ecercise

Characteristics of Isotopic and Elemental Compositions of Potential Water Pollution Sources in Rural Area

Byeong-Jun Jeon,Se-In Lee,Sang-Sun Lim,Kwang-Seung Lee,Hyun-Jin Park,Jong-Hyun Ham,Kwang-Sik Yoon,Sang-Mo Lee,Woo-Jung Choi 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

The Change of Glutathione Peroxidase Concentration with the Exercise Intensities during the Progressive Maximal Exercise Test

Jeon, Byeong-Hwan,Jun, Tae-Won,Woo, Jae-Hong,Park, Ik-Ryeul,Kim, Kwang-Jun,Suk, Min-Hwa 한국체육무용국제교류학회 2003 한국체육무용국제교류학회지 Vol.9 No.-

This study examines the change of plasma glutathione peroxidase (GRx) concentration during the progressive maximal exercise test performed on 9 untrained males(28.67±2.45yr). The VO_(2max) was determined through the pretest(treadmill running by Bruce protocol). On the main test, the blood of 9 untrained males was sampled on the rest state, the intensities leveled at 50%, 60%, 70%, 80%, 90% and 100% of VO_(2max), as well as 3 minutes and 6 minutes after the recovery. With the collected blood samples, the concentration of plasma GPx was analyzed by ELISA(Enzyme-linked immunosorbent assay). In addition, the changes of lactate concentration and energy expenditure were analyzed to compare with that of plasma GPx concentration. The statistical differences were determined by applying one-way ANOVA (p<0.05). The results suggest that there is no significant change in the plasma GPx concentration with the incremental exercise intensities. Furthermore, there are no noticeable relationships between the GPx concentration and the lactate cancentration. or the energy expenditure in their changes.

Purification and Some Properties of the Polyphenol Oxidase from Ascidian, Halocynthia roretzi

Byeong-Jun Jeon,Kang-Ho Lee,Hong-Soo Ryu,Byeong-Jin You 한국식품영양과학회 1996 Preventive Nutrition and Food Science Vol.1 No.1

Polyphenol oxidase(PPO) isolated from the crude extract of ascidian, Halocynthia roretzi, showed higher affinity for catechol than tyrosine or DL-DOPA. Successful enzyme assay could be performed at 25℃, 10 min. by mixing 0.2ml of crude enzyme extract with 2.8ml of 0.13M catechol in 0.1M sodium phosphate buffer (pH 6.4). The specific activity of PPO which had been purified with a combination of ammonium sulfate treatment, ion exchange chromatography on DEAE-cellulose, and gel filtration on Sepharose 6B was 13-fold higher than that of crude extract. The purified enzyme was homogeneous as confirmed by polyacrylamide disc gel electrophoresis. The activity of PPO was stable from pH 5.0 to 8.0 and showed the peak activity at pH 6.4. The optimum reaction temperature for PPO oxidation on catechol was 35℃ and those enzyme were heat stable up to 40℃. Molecular weight of the enzyme was estimated about 170 kDa. One molecule was found to be composed of four subunits. Two of them had molecular weight of 55 kDa and the others 30 kDa. The K_m values, V_(max) and catalytic efficiency(V_(max)/K_m) for catechol were 0.12mM, 2.56mM/liter/min. and 0.18min^(-1) respectively. The substrate affinity and electrophorectic pattern suggested that the enzyme of ascidian was considered to be not tyrosine but catechol oxidase.

High-performance gas sensors based on single-wall carbon nanotube random networks for the detection of nitric oxide down to the ppb-level

Jeon, Jun-Young,Kang, Byeong-Cheol,Byun, Young Tae,Ha, Tae-Jun The Royal Society of Chemistry 2019 Nanoscale Vol.11 No.4

<P>We demonstrate highly sensitive and selective gas sensors based on solution-processed single-wall carbon nanotube (SWCNT) random networks for the detection of nitric oxide (NO) down to the ppb-level operating at room temperature. The proposed gas sensors exhibited a response of 50% under both inert and air atmospheres with a theoretical detection limit of 0.2 ppb and a selectivity toward different target gases of volatile organic compounds, including benzene, toluene, and ammonia. The outstanding sensing performance was realized by functionalizing SWCNT random networks with polyethylenimine (PEI), which possesses a repeating structure of amine groups. We investigate the functionalization properties of SWCNT random networks by using atomic force microscopy, X-ray photoelectron spectroscopy and Raman spectroscopy and the sensing mechanism in the proposed NO gas sensors. We note that solution-process technologies, from the deposition of SWCNT random networks to the polymeric functionalization of amine groups, were employed at room temperature under an ambient atmosphere to fabricate highly sensitive and selective NO gas sensors, which are based on low-cost, effective, and scalable merits in the industry of sensors. We also investigate the effect of ultraviolet (UV) irradiation on the recovery time underlying the sensing mechanism. Photodesorption energy obtained by UV irradiation reduced the recovery time of the proposed NO gas sensors to within a few tens of seconds. We believe that this work is a promising and practical approach for realizing health-care monitoring systems by real-time analyzing NO gas at the ppb level in the field of biosensors.</P>

Pentaene macrolides AB023a and takanawaene C produced by Streptomyces xanthocidicus strain S3 for controlling pepper anthracnose

Jeon Byeong Jun,Kang Ji Eun,Do Kim Jeong,Kim Beom Seok 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-

A natural product library consisting of the culture extracts of 814 actinomycete strains was screened for antifungal compounds that disrupt the cell integrity of plant pathogenic fungi using an adenylate kinase (AK) assay system. The culture extract of Streptomyces xanthocidicus strain S3 exhibited high AK activity against various plant pathogens. The active ingredients, AT-1 and AT-2, were isolated from the culture extract using a series of chromatographic procedures. Based on MS, UV, and NMR spectrometric analyses, the structures of AT-1 and AT-2 were determined as the pentaene macrolides, AB023a and takanawaene C. AB023a and takanawaene C displayed broad-spectrum antifungal activity against Aspergillus oryzae, Botrytis cinerea, Colletotrichum coccodes, C. gloeosporioides, C. orbiculare, Cylindrocarpon destructans, and Fusarium oxysporum f. sp. lycopersici, showing minimum inhibitory concentrations of 1–32 μg/mL. Treatment of AB023a and takanawaene C successfully inhibited anthracnose development on pepper plants in a concentration-dependent manner without phytotoxicity. The disease control efficacy of both compounds was comparable to that of the commercial fungicide chlorothalonil. Collectively, these results suggest that the polyene macrolides produced by S. xanthocidicus strain S3 can be used as natural fungicides for plant disease control.

Purification and Some Properties of the polyphenol Oxidase form Ascidian, Halocynthia roretzi

Jeon, Byeong-Jun,Lee, Kang-Ho,Ryu, Hong-Soo,You, Byeong-Jin The Korean Society of Food Science and Nutrition 1996 Preventive Nutrition and Food Science Vol.1 No.1

Polyphenol oxidase(PPO) isolated from the crude extract of ascidian, Halocynthia roretzi, showed higher affinity for catechol than tyrosine or DL-DOPA. Successful enzyme assay could be performed at $25^{\circ}C$, 10min. by mixing 0.2ml of crude enzyme extract with 2.8ml of 0.13M catechol in 0.1M sodium phosphate buffer(pH 6.4). The specific activity of PPO which had been purified with a combination of ammonium sulfate treatment, ion exchange chromatography on DEAE-cellulose, and gel filtration on Sepharose 6B was 13-fold disc gel electrophoresis. The activity of PPO was stable from pH 5.0 to 8.0 and showed the peak activity at pH 6.4 .The optimum reaction temperature for PPO oxidation on catechol was 35$^{\circ}C$ and those enzyme were heat stable up to 4$0^{\circ}C$. Molecular weigth of the enzyme was estimated about 170kDa. One molecule was found to be composed of gour subunits. Two of them had molecular weigh of 55kDa and the others 30kDa. The {TEX}$K_{m}${/TEX} values, {TEX}$V_{max}${/TEX} and catalytic efficiency({TEX}$V_{max}${/TEX}/{TEX}$K_{m}${/TEX}) for catechol were 0.12mM, 2.5mM/liter/min. and {TEX}$0.18min^{-1}${/TEX} respectively. The substrate affinity and electrophorectic pattern suggested that the enzyme of ascidian was considered to be not tyosine but catechol oxidase.

동위원소비와 원소함량비 특성을 이용한 농촌 비점오염원 구명 타당성 평가

전병준 ( Byeong-jun Jeon ),이세인 ( Se-in Lee ),임상선 ( Sang-sun Lim ),이광승 ( Kwang-seung Lee ),박현진 ( Hyun-jin Park ),함종현 ( Jong-hyun Ham ),윤광식 ( Kwang-sik Yoon ),최우정 ( Woo-jung Choi ) 한국농공학회 2014 한국농공학회 학술대회초록집 Vol.2014 No.-

Water quality in rural areas is sensitive to a variety of non-point pollution sources from agricultural, livestock-farming, forest, and residential areas due to the multi-purposes land use characteristics. This study was conducted to characterize stable isotope and elemental compositions of potential pollution sources including livestock manure, nutrient inputs (synthetic fertilizer and compost), agricultural soils (paddy, upland, and orchard), and water samples (paddy drainage water, forest runoff water, and sewage waste water) to explore the potential use of isotopic and elemental compositions in estimating water pollution sources. Samples were collected from the Pungyoungjeong river watershed located in Jangseong of Chonnam Province, Korea. The samples were analyzed for concentrations of C and N and isotope ratio of C (δ¹³C) and N (δ¹⁵N) and metal (Ca, Mg, K, Na, Zn, Cu, and Al) concentrations. Each pollution sources have distinct isotopic (δ¹³C and δ¹⁵N) and elemental composition (Cu and Zn). For example, manure and its compost were characterized by higher isotopic values (δ¹³C and δ¹⁵N) and metal (Cu and Zn) concentration; whereas synthetic fertilizer had lower δ¹⁵N. The higher δ¹³C, δ¹⁵N, and metal concentration were attributed to feeding of less ¹³C-depleted corn, ¹⁴N loss from manure, and addition of Cu and Zn to formula feed, respectively. Upland and orchard soils had a higher δ¹³C and δ¹⁵N due to continuous application of ¹³C- and ¹⁵N-enriched livestock manure. Sewage waste water was characterized by a higher Na/Ca ratio reflecting excessive salt use in diet. The N/P ratio of forest runoff water was higher than other water samples probably due to forest soils where P fertilizer is not supplied. Therefore, our study suggests that the potential water pollution sources have contrasting isotopic and elemental compositions and thus it may be possible to use such characteristics in identifying water pollution sources.

IoT 를 활용한 골든 타임 보장 시스템

전병준 ( Byeong-jun Jeon ),연제성 ( Jae-sung Yeon ),김유나 ( Yuna Kim ),홍장의 ( Jang-eui Hong ),류관희 ( Kwan Hee Yoo ),나스리디노프아지즈 ( Nasridinov Aziz ) 한국정보처리학회 2016 한국정보처리학회 학술대회논문집 Vol.23 No.2

환자의 긍정적인 예후에 있어 골든 타임을 최대한 확보하는 것은 중요한 요소로 손꼽힌다. 현재 응급의료 체계에서는 사고가 일어난 직후부터 응급 차량이 도착하기 전까지 응급구조요원이 환자의 상태를 파악 할 수 있는 방법은 제보자가 전달한 정보로 한정된다. 따라서 환자 상태의 수치적 측정의 부재 및 부족한 환자 정보는 골든 타임 확보에 있어 중요한 응급 처치를 결정하는데 큰 어려움을 야기한다. 이 같은 문제를 해결하기 위해 본 논문은 사고 직후부터 응급 차량이 도착하기 전까지 응급 구조 요원이 도착하기 전까지 환자의 생체 징후를 파악할 수 있도록 하여 적절한 응급 처치를 예측할 수 있도록 돕는다. 또한 환자 주변에 전문지식을 보유한 사람이 없는 경우에도 환자의 상태에 따른 최소한의 조치를 취할 수 있도록 도움을 줌으로써 응급 차량이 도착하기 전까지 환자가 최상의 상태를 유지할 수 있도록 한다.