Characterization of the European type of maternal lineage evident in extant Jeju native pigs

Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.

지구성 운동이 자연발생 고혈압 흰쥐의 혈압에 미치는 영향

이규승,전병화,김일곤,전종귀 忠南大學校體育科學硏究所 1996 體育科學硏究誌 Vol.14 No.1

The purpose of this study is to investigate the effects of indurance exercise on BP of SHR Experimental animals were SHR(180) and SD(90) and they were divided into three groups ; a experimental group1( practice group), a experiment group2(non practice group), and a comparative group (normal group). The experimental group 1 was practicing on a treadmill at 15m/min exercise intensity for 30 minutes a day four times per week for eight weeks. While raising experimental animals, We measured BP for each group once a week, and took the weight of the heart after killing a rat. and then we examined the concentration of ?? and ??, compared, and analyzed them. we have reached the following results. 1. As time passed, BP in the experimental group 1 was almost equal to BP eight weeks ago. BP in the experimental group 2 continued to rise and then showed typical hypertension. 2. The three groups were inclined to increase the weight of their hearts as time passed. However, the experimental group 2 presented the weight of hearts increasing compared with the experimental group1 after eight weeks. 3. In the experimental group 1, and experimental group 2 groups, ?? concentration was but ?? concentration increased as time passed. However, the experimental group1 showed fewer changes than the experimental group 2. 4. The weight of all three groups were inclined to increase as time passed, but it didn't present any noteworthy differences. According to the analysed result of the above experiment, endurannsce exercise becomes an important factor in preventing the raising of BP of SHR, and the hypertrophy of hearts. Key Words : Spontaneously Hypertension Rats, Blood Pressure, Endurance Ecercise

The Change of Glutathione Peroxidase Concentration with the Exercise Intensities during the Progressive Maximal Exercise Test

Jeon, Byeong-Hwan,Jun, Tae-Won,Woo, Jae-Hong,Park, Ik-Ryeul,Kim, Kwang-Jun,Suk, Min-Hwa 한국체육무용국제교류학회 2003 한국체육무용국제교류학회지 Vol.9 No.-

This study examines the change of plasma glutathione peroxidase (GRx) concentration during the progressive maximal exercise test performed on 9 untrained males(28.67±2.45yr). The VO_(2max) was determined through the pretest(treadmill running by Bruce protocol). On the main test, the blood of 9 untrained males was sampled on the rest state, the intensities leveled at 50%, 60%, 70%, 80%, 90% and 100% of VO_(2max), as well as 3 minutes and 6 minutes after the recovery. With the collected blood samples, the concentration of plasma GPx was analyzed by ELISA(Enzyme-linked immunosorbent assay). In addition, the changes of lactate concentration and energy expenditure were analyzed to compare with that of plasma GPx concentration. The statistical differences were determined by applying one-way ANOVA (p<0.05). The results suggest that there is no significant change in the plasma GPx concentration with the incremental exercise intensities. Furthermore, there are no noticeable relationships between the GPx concentration and the lactate cancentration. or the energy expenditure in their changes.

Pentaene macrolides AB023a and takanawaene C produced by Streptomyces xanthocidicus strain S3 for controlling pepper anthracnose

Jeon Byeong Jun,Kang Ji Eun,Do Kim Jeong,Kim Beom Seok 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-

A natural product library consisting of the culture extracts of 814 actinomycete strains was screened for antifungal compounds that disrupt the cell integrity of plant pathogenic fungi using an adenylate kinase (AK) assay system. The culture extract of Streptomyces xanthocidicus strain S3 exhibited high AK activity against various plant pathogens. The active ingredients, AT-1 and AT-2, were isolated from the culture extract using a series of chromatographic procedures. Based on MS, UV, and NMR spectrometric analyses, the structures of AT-1 and AT-2 were determined as the pentaene macrolides, AB023a and takanawaene C. AB023a and takanawaene C displayed broad-spectrum antifungal activity against Aspergillus oryzae, Botrytis cinerea, Colletotrichum coccodes, C. gloeosporioides, C. orbiculare, Cylindrocarpon destructans, and Fusarium oxysporum f. sp. lycopersici, showing minimum inhibitory concentrations of 1–32 μg/mL. Treatment of AB023a and takanawaene C successfully inhibited anthracnose development on pepper plants in a concentration-dependent manner without phytotoxicity. The disease control efficacy of both compounds was comparable to that of the commercial fungicide chlorothalonil. Collectively, these results suggest that the polyene macrolides produced by S. xanthocidicus strain S3 can be used as natural fungicides for plant disease control.

High-performance gas sensors based on single-wall carbon nanotube random networks for the detection of nitric oxide down to the ppb-level

Jeon, Jun-Young,Kang, Byeong-Cheol,Byun, Young Tae,Ha, Tae-Jun The Royal Society of Chemistry 2019 Nanoscale Vol.11 No.4

<P>We demonstrate highly sensitive and selective gas sensors based on solution-processed single-wall carbon nanotube (SWCNT) random networks for the detection of nitric oxide (NO) down to the ppb-level operating at room temperature. The proposed gas sensors exhibited a response of 50% under both inert and air atmospheres with a theoretical detection limit of 0.2 ppb and a selectivity toward different target gases of volatile organic compounds, including benzene, toluene, and ammonia. The outstanding sensing performance was realized by functionalizing SWCNT random networks with polyethylenimine (PEI), which possesses a repeating structure of amine groups. We investigate the functionalization properties of SWCNT random networks by using atomic force microscopy, X-ray photoelectron spectroscopy and Raman spectroscopy and the sensing mechanism in the proposed NO gas sensors. We note that solution-process technologies, from the deposition of SWCNT random networks to the polymeric functionalization of amine groups, were employed at room temperature under an ambient atmosphere to fabricate highly sensitive and selective NO gas sensors, which are based on low-cost, effective, and scalable merits in the industry of sensors. We also investigate the effect of ultraviolet (UV) irradiation on the recovery time underlying the sensing mechanism. Photodesorption energy obtained by UV irradiation reduced the recovery time of the proposed NO gas sensors to within a few tens of seconds. We believe that this work is a promising and practical approach for realizing health-care monitoring systems by real-time analyzing NO gas at the ppb level in the field of biosensors.</P>

Purification and Some Properties of the polyphenol Oxidase form Ascidian, Halocynthia roretzi

Jeon, Byeong-Jun,Lee, Kang-Ho,Ryu, Hong-Soo,You, Byeong-Jin The Korean Society of Food Science and Nutrition 1996 Preventive Nutrition and Food Science Vol.1 No.1

Polyphenol oxidase(PPO) isolated from the crude extract of ascidian, Halocynthia roretzi, showed higher affinity for catechol than tyrosine or DL-DOPA. Successful enzyme assay could be performed at $25^{\circ}C$, 10min. by mixing 0.2ml of crude enzyme extract with 2.8ml of 0.13M catechol in 0.1M sodium phosphate buffer(pH 6.4). The specific activity of PPO which had been purified with a combination of ammonium sulfate treatment, ion exchange chromatography on DEAE-cellulose, and gel filtration on Sepharose 6B was 13-fold disc gel electrophoresis. The activity of PPO was stable from pH 5.0 to 8.0 and showed the peak activity at pH 6.4 .The optimum reaction temperature for PPO oxidation on catechol was 35$^{\circ}C$ and those enzyme were heat stable up to 4$0^{\circ}C$. Molecular weigth of the enzyme was estimated about 170kDa. One molecule was found to be composed of gour subunits. Two of them had molecular weigh of 55kDa and the others 30kDa. The {TEX}$K_{m}${/TEX} values, {TEX}$V_{max}${/TEX} and catalytic efficiency({TEX}$V_{max}${/TEX}/{TEX}$K_{m}${/TEX}) for catechol were 0.12mM, 2.5mM/liter/min. and {TEX}$0.18min^{-1}${/TEX} respectively. The substrate affinity and electrophorectic pattern suggested that the enzyme of ascidian was considered to be not tyosine but catechol oxidase.

Purification and Some Properties of the Polyphenol Oxidase from Ascidian, Halocynthia roretzi

Byeong-Jun Jeon,Kang-Ho Lee,Hong-Soo Ryu,Byeong-Jin You 한국식품영양과학회 1996 Preventive Nutrition and Food Science Vol.1 No.1

Polyphenol oxidase(PPO) isolated from the crude extract of ascidian, Halocynthia roretzi, showed higher affinity for catechol than tyrosine or DL-DOPA. Successful enzyme assay could be performed at 25℃, 10 min. by mixing 0.2ml of crude enzyme extract with 2.8ml of 0.13M catechol in 0.1M sodium phosphate buffer (pH 6.4). The specific activity of PPO which had been purified with a combination of ammonium sulfate treatment, ion exchange chromatography on DEAE-cellulose, and gel filtration on Sepharose 6B was 13-fold higher than that of crude extract. The purified enzyme was homogeneous as confirmed by polyacrylamide disc gel electrophoresis. The activity of PPO was stable from pH 5.0 to 8.0 and showed the peak activity at pH 6.4. The optimum reaction temperature for PPO oxidation on catechol was 35℃ and those enzyme were heat stable up to 40℃. Molecular weight of the enzyme was estimated about 170 kDa. One molecule was found to be composed of four subunits. Two of them had molecular weight of 55 kDa and the others 30 kDa. The K_m values, V_(max) and catalytic efficiency(V_(max)/K_m) for catechol were 0.12mM, 2.56mM/liter/min. and 0.18min^(-1) respectively. The substrate affinity and electrophorectic pattern suggested that the enzyme of ascidian was considered to be not tyrosine but catechol oxidase.

Characteristics of Isotopic and Elemental Compositions of Potential Water Pollution Sources in Rural Area

Byeong-Jun Jeon,Se-In Lee,Sang-Sun Lim,Kwang-Seung Lee,Hyun-Jin Park,Jong-Hyun Ham,Kwang-Sik Yoon,Sang-Mo Lee,Woo-Jung Choi 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

특집논문 : 박인환과 김수영의 시에 나타난 신의 의미 연구

전병준 ( Byeong Jun Jeon ) 국제비교한국학회 2013 비교한국학 Comparative Korean Studies Vol.21 No.3

본 논문은 박인환과 김수영의 시를, 신적인 것을 다룬 시를 중심으로 살핀 논문이다. 이들은 모두 해방 이후의 혼란과 자유를 겪었고, 전쟁이라는 극단을 체험하였다. 비록 궁극적인 시적 관심에서는 상이했다고 하더라도 서로 경쟁하는 관계에 있었던 까닭에 1950년대 시사의 전개 과정에서 이들을 비교하여 살피는 것은 필수적인 작업이다. 단순히 비난과 폄하의 관계로 치부해서는 이들의 시학을 제대로 규명할 수 없을 뿐 아니라 1950년대 시사 자체도 기술될 수 없다. 이런 문제의식 아래 이들을 동시에 살필 수 있는 관점의 하나로 신적인 것에 대한 관심을 드러낸 시를 살핀 것이다. 박인환에게 신, 혹은 신적인 것이 시에 도입되는 과정은 전적으로 전쟁과 관련된다. 전쟁의 참화를 겪으며 그는 인간적인 것뿐 아니라 신적인 것에 대해서도 절망했다. 그러나 그런 절망적인 상황에서도 삶을 지탱할 수 있는 최소한의 희망이 필요했을 터이니 비록 비관적인 전망이라 하더라도 신적인 것을 매개로 그러한 전망을 가지게 된 것은 필연적인 과정이었다고 하겠다. 김수영에게 신적인 것이란 인간적인 것을 반성적으로 사유하게 해주는 계기로 작용하였다. 신적인 것이라는 절대적 타자를 통해 그는 좀 더 치열한 자기 성찰에 이르게 되니, 이를 통해 현실에 대한 냉정한 판단을 할 수 있었으리라 생각된다. 자기가 발 딛고 있는 세계에 대해 절망하였으나 오히려 자신과 세계에 대한 절대적인 타자를 상정함으로써 그 절망을 딛고 일어설 수 있는 가능성을 발견하였으리라 추측하는 것이 지나치지는 않을 것이다. 김수영에게 신, 혹은 신적인 것이란 타자를 통해 자신을 깨치게 하는 중요한 자극과 계기의 하나가 되는 것이다. 본 논문은 박인환과 김수영을 신이라는 주제를 중심으로 살폈다. 이러한 관점이 1950년대를 중심으로 하는 한국현대시사의 전개 과정에서 박인환과 김수영을 동시에 살필 수 있는 유효한 관점의 하나로 판단했기 때문이다. 이러한 관점이나 시야는 박인환과 김수영을 함께 연구하는 데 유일한 것이 될 수 없음은 주지의 사실이지만 이러한 연구가 축적됨으로써 1950년대의 한국시사가 새로이 쓰일 수 있는 것 또한 부정할 수 없는 사실이다. 본 논문에서 시론적으로 제기한 관점을 다양한 측면에서 검토하고, 이와 더불어 박인환과 김수영의 시를 살필 수 있는 적절한 관점과 방법론을 제기하는 것이 추후의 과제라 하겠다. This thesis tried to clarify the meaning of God in poems of In-whan Park and Su-young Kim which is thoght to be the key concept to understand their poetic core under the 1950`s. The process of God into the poems of Park is wholly related to Korean War. Although he despaired about the human as well as the divine, he managed to endure taking hope. The role of God in Kim`s poems is that it functioned as the other which made him to come to recognize himself. It made him the dialectics of the finite and the infinite, and finally helped the realizational thought.

비점원 오염 물질의 동위원소 및 원소함량의 특성을 이용한 오염원 구명 가능성 평가

전병준 ( Byeong-jun Jeon ),이세인 ( Se-in Lee ),이광승 ( Kwang-seung Lee ),임상선 ( Sang-sun Lim ),박현진 ( Hyun-jin Park ),함종현 ( Jong-hyun Ham ),윤광식 ( Kwang-sik Yoon ),최우정 ( Woo-jung Choi ) 한국농공학회 2013 한국농공학회 학술대회초록집 Vol.2013 No.-

복합토지이용 유역은 농경지, 축산, 산림, 생활계 등 다양한 비점오염원이 산재해 있어 하천에 유입되는 비점오염물질의 배출 경로를 파악하기가 어렵다. 본 연구는 영농ㆍ축산ㆍ주거가 복합적으로 구성된 전라남도 장성군 소재의 풍영정천 유역에서 강우시 하천 유입이 가능한 오염 원인 물질인 경작지 토양(논, 밭, 과수원), 사료(임신우사료, 우사료, 계사료), 볏짚, 낙엽, 가축분(우분, 계분), 퇴비 시료를 채취하여 동위원소 및 원소함량을 분석하고 오염 지표로서의 활용 가능성을 평가하였다. 토양의 경우 밭(탄소 : -25.4±0.3‰, 질소 : 9.6±0.5‰)과 과수원(탄소 : -24.2±0.8‰, 질소: 10.6±0.4‰)의 탄소, 질소동위원소비 모두 논(탄소 : -29.6±0.2‰, 질소 : 4.9±0.2‰)보다 높았는데, 이는 밭과 과수원에 탄소동위원소비가 높은 퇴비(탄소 : -24.2±1.4‰, 질소 : 16.3±0.3‰) 시용량이 많기 때문으로 판단된다. 또한, 논토양의 질소동위원소비가 낮은 이유는 혐기적 조건으로 질산화속도가 느리게 진행되어 질산염 용탈이나 탈질에 의한 질소손실이 낮고, 반면에 미생물에 의한 부동화와 재무기화가 순환적으로 일어나기 때문으로 판단된다. 토양의 총탄소, 총질소, 무기태 질소, 총인, 수용성인, 기타 금속 함량은 논, 밭, 과수원 토양에서 유의미한 차이가 없었고, 토양의 Al농도가 타 오염원보다 높았다. 농업/축산 자재 및 농림축 부산물의 탄소동위원소비 중 사료의 탄소동위원소비(-19.7±0.8‰)가 볏짚(-28.2±0.1‰)과 낙엽(-28.3±0.3‰)보다 높았는데, 이는 배합사료의 주원료가 C4 식물인 옥수수이기 때문이며, 이는 가축분뇨의 탄소동위원소비(-23.1±1.8‰)와도 상응한다. 질소동위원소비의 경우 퇴비(16.3±0.3‰)가 화학비료(-1.5±0.5‰)보다 매우 높았고, 산림 부산물인 낙엽(-0.7±0.3‰)이 농림축 부산물인 볏짚(8.1±0.9‰)과 가축분뇨(10.2±3.5‰)보다 낮았다. 그리고 농업/축산 자재 및 농림축 부산물의 원소함량 중 분뇨의 탄질비(20.3±2.8)가 퇴비(14.6±1.9)보다 높았는데, 이는 퇴비화 과정 중에서 CO₂ 손실에 의해 퇴비의 탄질비가 낮아졌기 때문으로 판단된다. 또한, 퇴비, 볏짚, 낙엽의 Al/(Ca+K+Mg+Na)는 각각 0.027, 0.031, 0.939로 토양(논 5.8, 밭 5.8, 과수원 6.4)보다 낮았고, (Cu+Zn)/Al는 퇴비(0.6)가 볏짚(0.09), 낙엽(0.14) 및 토양(논 0.002, 밭 0.002, 과수원 0.001)보다 상대적으로 높았다. 이와 같은 경작지 유형별 토양, 농업/축산 자재 및 농림축 부산물의 탄소, 질소동위원소비와 금속함량 차이를 이용하여 수질 비점오염의 원인과 경로를 파악할 수 있을 것으로 기대된다.