Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

가스화 - 수소 회수 공정 개발을 위한 예비실험

차재준(Jae-Jun Cha),김영석(Young-Suk Kim),이진호(Jin-Ho Lee),황순모(Soon-Mo Hwang),정성재(Seong-Jae Jeong) 한국열환경공학회 2010 한국열환경공학회 학술대회지 Vol.2010 No.춘계

Polylysine을 입힌 배양면이 GH₃ 뇌하수체 종양 세포에 미치는 영향에 관한 연구

김정진,백상호,황덕호,황영일,이왕재,이병란 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

골격근 근형질세망의 ATP유도 ^45Ca-uptake에 대한 Thapsigargin및 Vanillylnonanamide의 영향

황의강,배소현,홍장희,허강민,김진회,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To investigate the effect of thapsigargin(THP) and vanillylnonanamide(VN), derivative of capsaicin, on the ATP-induced ^45Ca-uptake in the sarcoplasmic reticulum(SR) of the skeletal muscle, the SR vesicles were prepared from the back muscle of the rabbit, and ^45Ca-uptake was carried out. The results as follows: ATP-induced ^45Ca-uptake of skeletal muscular SR was significantly increased by 1 mM ATP. It was significantly blocked by 5 μM THP, but slightly decreased by 20 μM VN. The combined effect of THP and VN on the 45Ca-uptake of the SR vesicles was much potentiated than the sum of each effect of them. The above results suggest that the action of VN, being slightly influential to ATP-induced Ca-uptake but potentiating the effect of THP on the Ca-uptake, might be related with direct perturbation of the SR membrane or exposure of the THP-binding site.

프랑스 1958年(現行)憲法의 改正

鄭在晃 홍익대학교 법학연구소 2000 법학연구 Vol.2 No.-

〈La révision de la Constitution de la République française〉 Il y avait plusieurs révisons importantes de la Constitution 1958 en France : la révison pour la ratification du traité sur l´Union européenne(Maastricht) ; la révison qui a modifié la composition et les pouvoirs du Conseil supérieur de la magistrature pour renforcer l'indépendance des magistrates ; la révison pour l'extension du champ du référendum et l'introduction du principe d'une session parlementaire unique ordinaire ; ka révison pour favoriser l'égal accès des femmes et des homes aux mandates électoraux et functions électives ; la révison pour la réduction du mandat présidentiel(quinquennat) etc. Ces révisons de la Constitution française n'ont pas beaucoup été étuciées en Corée. Ce qui nous a conduit a` traiter sur les procédures de révison, les motifs des révisons importantes et ses contenus en droit constitutionnel français, Dans cette étude nous avons essyé de constater des expériences françaises auxquelles il ne serait pas moins utile de se référer pour le droit constitutionnel coréen.

條約에 대한 違憲審査의 問題

鄭在晃 弘益大學校 法學硏究所 1999 법학연구 Vol.1 No.-

Il y a des de´cisions de la Cour constitutionnelle core´enne relatives aux traite´(accord)s parmi lesquelles l'on trouve und de´cision qui constate directement, dans son dispositif, la constitutionnalite´d'un traite´. En vertu de l'article 6 premier aline´a de la Constitution core´enne, les traite´s ratifie´s par la constitution ont la me´me valeur que celle des re`gles juridiques domstiques. En Core´e il exist plusieurs sortes des re`gles juridiques domstiques : Constitution, lois, re`glements etc. Les auteurs core´ens admettent qu'il y a ou bien les traite´s a` valeur le´gislative, ou bien les trarte´(accord)s a´ valeur re´glementaire, ou bien les traite´s a` valeur constitutionnelle. Selon cette classification, les modalite´s ou les voies de la constitutionnalite´ des traitel´s peuvent e^tre diverses. Nous avons e´tudie´ ces modalite´s et leurs proce´dures. Premie`rement, aux termes de l'article 107 premier aline´a et de l'article 111 premier aline´a de la Constitution core´enne, c'est la Cour constitutionnelle core´enne qui a le pouvior de de´cider de la constitutionnalite´ des lois. Par conse´quent, les auteurs core´ens admettent que sont susceptibles d'e^tre examine´s par la Cour constitutionnelle core´enne les traite´s importants soumis au consetement du Parlement aux termes de l'article 60 de la Constitution core´enne dans la mesure ou`l'on pourrait penser que le consentement du Parlement e´quivaut a` la le´gislation. Deuxie`mement, les auteurs core´ens admettent que la conformite´a` la Constitution des traite´(accord)s qui ont une valeur infe´rieure a` la Consitution des traite´(accord)s qui ont une valeur infe´rieur a`la loi peut e^tre examine´e, en principe par la Cour supre^me et les tribunaux judiciaires core´ens. C'est parce qu'aux termes de l'article 107 aline´a 2 de la Constitution core´enne ce sont la Cour Supre^me et les tribunaux judiciaires core´ens qui ont le pouvoir de de´cider de la constitutionnalite´ des de´cre^ts, des arre^s et des actes administratifs. En Core´e, il existe d'autre possibilite´ de voie du contro^le de la constitutionnalite´ des traite´(accord)s. C'est celle du recours constitutionnel par lequel peuvent e^tre contro^le´s les traite´(accord)s qui porteraient atteinte directement(sans l'acte de i'exe´cution de ces traite´s par l'administration) aux droits fondamentaux d'un individu. Mais le contro^le de la constitutionnalite´ des traite´s pourrait avoir des inconve´nients parce que le contro^le de la constitutionnalite´ est exerce´ a posteriori en Core´e. A cet e´gard nous avons consulste´ le syste`me francais ou` le contro^le de la constitutionnalite´ des traite´s est exerce´ a priori.

시각적 감도를 고려한 영상의 비트량 제어

황재정,오성택 群山大學校 1996 論文集 Vol.23 No.-

Image data is necessary to be reduced to solve the problem of storage media capacity and to efficiently use channel bandwidth for storing and transmiting multimedia data. In this study, we present an image coder using variable quantizer for the JPEG extensions named as ISO/IEC 10918-3 (also as ITU-T Rec. T.84). It is necessary to alleviate the blocking artifact which is more sensitive to human eye in view of the spatial frequency sensitivity and to visually control bit allocation in an image. Since the blocking artifact arises in the lower activity area rather than in the higher area. The block activity is referred to quantize a block and to control buffer status. An image is composed of slices, while a slice is composed of 8 ×8 blocks. Hence we optimize the bit assignment in an image at a given bit rate. As a result of simulation for various images, the proposed coder increases subjective and objective quality.

Diphenylhydantoin이 백서뇌신경세포막의 Na^+, K^+ -ATPase에 미치는 영향

황인방,허강민,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

To study the effect of diphenylhydantoin on the synaptosomal Na^+ K^+ -ATPase in the rat with Goldblatt kidney, one kidney was removed and renal artery on the other side was partially ligated (external diameter=0.38mm). One kidney was removed in the control group. Synaptosome was prepared from the control and hypertensive rat and ATPase activity (total, EGTA, Azide and ouabain sensitive) was measured in the presence or absence of saponin. The effect of diphenylhydantoin on the synaptosomal ATPase was observed in the presence of diphenylhydantoin and/or ouabain. To investigate the maximal binding of ouabain to synaptosome, ouabain binding study was also carried out. Results as follows: 1. Blood pressure was significantly increased in 1-kidney, 1-clip hypertensive rat. 2. ATPase activity(Total, EGTA and ouabain sensitive) was increased to 2 times in the presence of saponin in the hypertensive and control group. 3. Na^+, K^+ -ATPase activity in hypertensive group was significantly decreased, compared with the control group. 4. Diphenylhydantoin slightly increased the ATPase activity in the control and hypertensive group, but it was not significant. 5. Total activity of ATPase was significantly decreased in the case with dephenylhydantoin and ouabain, but there was not difference between ouabain and ouabain with diphenylhydantoin treated case. 6. In the experiment of oubain binding, diphenylhydantoin did not alter Bmax and Kd of hypertensive and control group. From the above results, it is suggested that diphenylhydantoin will decrease the intracellular sodium without dependence on the stimulation of Na^+, K^+ -ATPase-in hypertensive rat.

주파수 하중 Hadamard 변환 부호화

황재정,이문호 군산대학교 1987 群山水産專門大學 硏究報告 Vol.21 No.2

Digitizer system which is able to interface analog image signal with IBM PCXT is deviced and we processed digital data through Hadamed Transform Coding. Incorporaion of model of the human visual system into an image compression or quality assessment technique intuitively should (and has often proven to) improve performance. Clearly, the, it should prove highly beneficial to combine the image transform with a visual model. In the past, combining these two has been hindered by a fundermental problem resulting from the scene alteration that is necessary for proper transform utilization. A new analytical solution to this problem, taking the form of a straightforward multiplicative weighting function, is developed in this paper. Excellent performance is demonstrated in terms of mean square error and subjective assessment. Results are presented at a total rate of 0.5 bit/pixel for a stationary monochrome image.