New polymorphic microsatellite markers in the Korean mi-iuy croaker, $Miichthys$ $miiuy$, and their application to the genetic characterization of wild and farmed populations

An, Hye-Suck,Kim, Eun-Mi,Lee, Jang-Wook,Kim, Dae-Jung,Kim, Yi-Cheong The Korean Society for Integrative Biology 2012 Animal cells and systems Vol.16 No.1

Eighteen new polymorphic microsatellite markers were developed for the Korean mi-iuy croaker ($Miichthys$ $miiuy$, Perciformes, Sciaenidae), and allelic variability was compared between a wild population in Mokpo, Korea, and a hatchery population in Tongyeong, Korea. All loci were amplified readily and demonstrated allelic variability, with the number of alleles ranging from 5 to 37 in the wild population, and from 4 to 12 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, to be 0.74 and 0.78 in the hatchery population samples, and 0.79 and 0.86 in the wild samples. These results indicate lower genetic variability in the hatchery population compared with the wild population, and significant genetic differentiation between the wild population and the hatchery samples ($F_{ST}$=0.058, P<0.001). These microsatellite loci may be valuable for future population genetic studies, monitoring changes in the genetic variation within stocks in a commercial breeding program, conservation genetics, and molecular assisted selective breeding of the mi-iuy croaker in the future.

VRE 감염증의 임상상, 미생물학적 특성 분석 및 유전적 관련성 비교 : 전국 6개 병원에서 분리된 VRE를 중심으로

정희진,박정원,엄중식,하경임,신종희,김정미,이혜수,박영희,김우주,박승철 대한감염학회 2002 감염 Vol.34 No.5

천연 생약재 열수 및 알코올 추출물의 어병 세균에 대한 항균력

최혜승,김이청,이주석,조미라,서창호,박수일 한국어병학회 2004 한국어병학회지 Vol.17 No.1

감초 외 48종의 천연 생약제로부터 열수 및 알콜 추출액을 제작하여 19 균주 어병 세균을 대상으로 항균력을 조사한 결과, 알콜 추출액은 22종, 열수 추출액은 16종이 어병 세균에 대하여 항균력을 나타내며 이 중 13종의 생약재는 알콜 및 열수 추출액 모두 8㎜ 이상의 저지대를 나타내어 항균력이 있는 것으로 나타났다. 열수 추출물에서 항균력을 나타내는 약재 중 그람 음성균에 감수성이 양호한 약재는 저지대 8 ㎜ 이상을 나타내는 애엽, 개옻나무, 연교, 지유, 파엽, 대황 및 황금이었으며, 그람 양성균에 항균력이 양호한 약재는 삼지구엽초, 육계 및 보골지 등 이었다. 그리고 오매, 황련, 계혈등, 상백피, 오배자 및 오미자는 저지대가 8㎜ 이상으로 그람 음성,양성균 모두에 항균력이 있었다. 알콜 추출물중에서 그람 음성균에 항균력을 나타내는 것은 백작약, 오매, 선모 및 황금 등으로 저지대가 8㎜ 이상으로 측정되었으며, 그람 양성균에 항균력을 나타내는 것은 감초, 계혈등, 단삼, 상백피, 육계 및 보골지 등으로 저지대가 8㎜ 이상이었다. 또한 그람 음성, 양성균에 모두 8㎜ 이상의 저지대를 나타내는 것은 애엽, 개옻나무, 황련, 지유, 오배자 및 오미자 등이었다. 그러나 이 중에서 오배자 열수 추출물의 어병 세균에 대한 발육 저지대가 32㎜로 그람 음성, 양성균 모두에 가장 뛰어난 항균력을 나타내어 다른 생약재와 다른 광범위 생약재인 것으로 나타났다. 애엽, 황금, 지유, 오매, 황련 및 오배자 열수 ·알콜 추출물은 tetracycline에 내성을 나타내는 균주에 감수성을 나타내었다. Hundreds of medicinal herbs have been using for the purpose of diseases treatment and immune enhancement for human being and other animals including fishes. Among them, 49 species of medicinal herbs were selected and tested for antibacterial activities against 19 strains of fish pathogenic bacteria in different 4 species. The 49 medicinal herbs were extracted by water and ethyl alcohol. The extracts were freeze dried and some paper discs from the extracts were prepared for the evaluation of antibacterial activity. The tested pathogenic bacteria were 5 strains of Edwardsiellu tarda, 5 strains of Vibrio sp., 4 strains of Lactococcus gawieae, 1 strain of Lactococcus raffinose. 1 strain of Streptococcus parauberius, and 3 strains of Streptococcus iniue. The Galla rhois (Obaeja), Gaeonnamu and Hwangleyon showed antibacterial activities on both gram negative and gram positive fish pathogenic bacteria. The Youkgae, Sangbaekpi, Bogolji and Gamcho showed very effective antibacterial activities on gram positive pathogens while Jiyu, Aeyoeb and Yeonkyo showed very effective on gram negative pathogens.

중등학생들의 기후 소양 함양을 위한 교수 자료 개발 및 현장 적용에 관한 연구

박종근,정철,손미희,육혜경 大邱大學校 師範大學 附設 敎育硏究所 2010 學校敎育硏究 Vol.5 No.2

본 연구는 국⋅내외적으로 기후 변화에 대한 관심의 증대와 기후 변화 교육에 대한 필요성이 요구되는 시점에서 중등학생의 기후 소양 함양을 위한 교수 자료의 개발과 중등학교 현장에서의 적용 가능성을 파악하는 데 그 목적이 있다. 이를 위하여 국내⋅외 기후 변화 교육의 현황을 조사하였으며, 기후 변화 교육 연구와 관련한 선행 연구들을 조사 분석하여 중등학생의 기후 소양 함양을 위한 교육 목표와 교육 내용 체계를 수립한 후 현장에 적용 가능한 방안을 제시하였다. 연구 결과는 다음과 같다. 첫째, 기후 소양 교육 목표를 “기후 변화에 따른 환경 변화를 인식하고, 탐구를 통하여 기후 변화에 관한 종합적인 이해와 의사소통 능력을 함양하여 기후 변화 대응 및 적응을 위한 방안을 제시하며, 이 를 지속적으로 실천한다”로 설정하였다. 둘째, 기후 소양 교육 내용의 영역을 ‘기후 변화 과학의 원리’, ‘기후 변화와 인간 생활’, ‘기후 변화의 대응 및 적응’의 3가지 대영역으로 설정하였다. 셋째, 기후 소양 교육 목표와 내용 체계를 중등학교 교육과정에 활용할 수 있도록 대영역별로 내용 기준을 구체화하였다. Facing the issue of global climate change, it is important for educational community to help our students get the climate literacy with which to guide their own thoughts and decisions in a changing world. Therefore, climate change education is to help learners understand of their influence on climate and climate's influence on them and society. The purpose of this study was to develop the teaching materials for climate literacy cultivation in secondary school students. The results of this study are as follows. Firstly, The goal of climate literacy education is ‘to perceive the changing environment based on the phenomena of climate change, to the proposed plans for response and adapt to climate change through the cultivation of communication competence and the overall understanding about climate change through inquiry, and to keep practicing the proposed plans as a member of a community for global environment.' Secondly, contents of some programs and guidelines were compared and analyzed, and then fields of educational contents were created and items by fields were drawn out. The content system of climate literacy is composed of ‘principle of climate change science’, ‘climate change and human activity, ‘response and adaptation of climate change’. Thirdly, concrete content standards suitable for secondary school curriculum were suggested so that the goal and content system for climate literacy education.

국내 의료종사자에서 HHV-8의 항체양성률에 대한 연구

주우철,최용준,박재은,이혜명,이진수,정문현,김수미,문지혜 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

HHV-8 virus is known to be associated with Kaposi's sarcoma (KS) in HIV seropositive patients and its seroprevalence has geographic difference, In South Korea, incidence of KS is low not only in general population but also in HIV patients compared to other countries, It is speculated that low seroprevalence of HHV-8 has contributed to the low incidence of KS in South Korea. We examined the seroprevalence of anti HHV-8 IgG of HIV seronegative health care workers in one University hospital. Enzyme-linked immunosorbent assay(ELISA) was used for diagnosis. One person (0.6% 1/164) was seropositive, six were equivocal (3.7%,6/164) and 157 people were seronegative (95.73%, 157/164). This study revealed that seroprevalence of HHV-8 in general population was. low in South Korea. Further studies are needed to be carried out to evaluate the low HHV-8 seroprevalence in Korea.

Association Analysis between Insulin-like Growth Factor Binding Protein 3 (IGFBP3) Polymorphisms and Carcass Traits in Cattle

Cheong, Hyun Sub,Yoon, Du-Hak,Kim, Lyoung Hyo,Park, Byung Lae,Lee, Hye Won,Namgoong, Sohg,Kim, Eun Mi,Chung, Eui Ryong,Cheong, Il-Cheong,Shin, Hyoung Doo Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.3

The insulin-like growth factor binding protein 3 (IGFBP3) has been investigated as a candidate gene for growth promoting effects in beef cattle and a modulator of IGF bioactivity. Previously, we have reported twenty two sequence variants discovered in Korean native cattle (Hanwoo). In this study, we examined the association between gene-specific polymorphisms of IGFBP3 and cold carcass weight (CW) and marbling score (MS) among Korean native cattle. Among twenty two polymorphisms, four common polymorphic sites (-854G>C, -100G>A, +421G>T and +3863C>A) were genotyped in our beef cattle (n = 437). Statistical analysis revealed that one common polymorphism in the promoter region (-854G>C) showed putative associations with MS (p = 0.03). IGFBP3 variation/haplotype information analyzed in this study will provide valuable information into strategies for the production of a commercial line of beef cattle.

Changes in the perinatal outcome of twin pregnancies delivered at a tertiary referral hospital during a 20-year period of time from 1995 to 2014

( Hye Ran Lee ),( Soo Hyun Kim ),( Yoo Min Kim ),( Ji Hye Kim ),( Mi Na Kim ),( Hye Jung Lee ),( Kylie Hae Jin Chang ),( Ji Ye Kim ),( Ji Hee Sung ),( Soo Young Oh ),( Cheong Rae Roh ),( Jong Hwa Kim 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-

서울 일부 고등학생의 카페인 함유식품에 대한 인식 및 섭취 실태

정지혜 ( Ji-Hye Cheong ),최경아 ( Kyoung-A Choi ),김유미 ( Yu-Mi Kim ),김명희 ( Myung-Hee Kim ),최미경 ( Mi-Kyeong Choi ) 대한영양사협회 2021 대한영양사협회 학술지 Vol.27 No.3

The high caffeine intake by adolescents has been a concern. The purpose of this study was to examine the awareness and consumption of caffeine-containing foods among 443 high school students using a questionnaire. An analysis of the spending patterns of the students’ weekly allowance showed that the amounts spent on purchasing caffeine-containing foods were higher for female students than male students (P<0.001). The scoring of the perception of caffeine was 3.1 out of 5, interest in the caffeine content of food was 2.6, consumption of caffeine-containing foods was 2.6, and usefulness of caffeine-containing foods was 2.7. The awareness of caffeine content in food was significantly higher in females (7.3 out of 11) than male students (6.7) (P<0.01). Approximately 59% of students perceived that the relationship between caffeine-containing foods and health, was harmful, and the experience of side effects after taking caffeine was significantly higher in female students than males. These side effects include heartburn (P<0.001), headache or dizziness (P<0.001), irregular heartbeat (P<0.05), and hands and feet shake (P<0.01). Caffeine-containing foods were purchased at convenience stores (62.1%). The factor considered when purchasing caffeine-containing foods was taste (72.2%), and the use of nutrition labeling for caffeine-containing foods scored 2.0 out of 5 points. When assessing the intake of caffeine-containing foods, the foods consumed more than once a week were in the order of coke, chocolate, chocolate milk, chocolate pie, and chocolate bars. These results suggest that it is necessary to prepare a caffeine-related nutrition guide improvement by sales management, and strengthen food labeling standards for the desirable recognition of caffeine and its safe intake by adolescents.

당뇨병 환자의 임상영양치료를 위한 임상영양사의 직무표준 개발

권수진 ( Su Jin Gwon ),우미혜 ( Mi Hye Woo ),주달래 ( Dal Lae Ju ),김은미 ( Eun Mi Kim ),박미선 ( Mi Sun Park ),손정민 ( Cheong Min Sohn ),위경애 ( Gyung Ah Wie ),이송미 ( Song Mi Lee ),차진아 ( Jin A Cha ),서정숙 ( Jung Sook Seo 대한영양사협회 2015 대한영양사협회 학술지 Vol.21 No.1

This study was conducted to develop job standards for clinical dietitian administering clinical nutrition therapy to diabetic patients in hospitals. Based on DACUM (Developing A Curriculum) analysis of 17 members including clinical dietitians, professors majoring in clinical nutrition and researchers, information on duties, tasks and task elements of clinical dietitians for diabetes care were derived and applied to diabetes mellitus- specific clinical nutrition care in hospitals for evaluation. The final developed job standards for clinical dietitians for diabetes care included four duties, 19 tasks and 56 task elements. The duties consisted of nutrition assessment, nutrition diagnosis, nutrition intervention, and nutrition monitoringㆍevaluation. For application of diabetes mellitus-specific job standards in clinical nutrition care, 108 work activities were developed and classified into 90 basic and 18 recommended types. Performance rates of standardized jobs were 80.2% at nutrition assessment, 99.6% at nutrition diagnosis, 78.5% at nutrition intervention, and 32.9% at nutrition monitoringㆍevaluation. These results can be applied as guidelines to implement jobs for diabetes mellitus-specific clinical nutrition services in clinical settings. In addition, they would be useful for education standards in educational institutions for education and training of clinical dietitian.

Development of Job Standards for Clinical Nutrition Therapy for Dyslipidemia Patients

( Min-jae Kang ),( Jung-sook Seo ),( Eun-mi Kim ),( Mi-sun Park ),( Mi-hye Woo ),( Dal-lae Ju ),( Gyung-ah Wie ),( Song-mi Lee ),( Jin-a Cha ),( Cheong-min Sohn ) 한국임상영양학회 2015 Clinical Nutrition Research Vol.4 No.2

Dyslipidemia has significantly contributed to the increase of death and morbidity rates related to cardiovascular diseases. Clinical nutrition service provided by dietitians has been reported to have a positive effect on relief of medical symptoms or reducing the further medical costs. However, there is a lack of researches to identify key competencies and job standard for clinical dietitians to care patients with dyslipidemia. Therefore, the purpose of this study was to analyze the job components of clinical dietitian and develop the standard for professional practice to provide effective nutrition management for dyslipidemia patients. The current status of clinical nutrition therapy for dyslipidemia patients in hospitals with 300 or more beds was studied. After duty tasks and task elements of nutrition care process for dyslipidemia clinical dietitians were developed by developing a curriculum (DACUM) analysis method. The developed job standards were pretested in order to evaluate job performance, difficulty, and job standards. As a result, the job standard included four jobs, 18 tasks, and 53 task elements, and specific job description includes 73 basic services and 26 recommended services. When clinical dietitians managing dyslipidemia patients performed their practice according to this job standard for 30 patients the job performance rate was 68.3%. Therefore, the job standards of clinical dietitians for clinical nutrition service for dyslipidemia patients proposed in this study can be effectively used by hospitals.