2-Pyridone을 이용한 Nucleic Acid Bases간의 Multiple Hydrogen Bonding에 관한 연구

황정숙 대전대학교 기초과학연구소 2000 自然科學 Vol.11 No.2

핵산 염기간의 multiple hydrogen bonding model로서 2-pyridone의 N-methyl-2-pyrrolidone 에 대한 hydrogen bonding association을 25℃ 사염화 탄소 용액에서 측정하여 94 M^(?)의 값을 얻었다. Complexation constant 값을 고정한 후, pK_(a) of N-H acids와 bases의 log K 간의 Bronsted relationship을 이용하여, 2-Pyridone의 dimerization constant 값 k_(d)를 계산한 결과 1.2×10^(5) M^(-1)로 나왔다. 2-pyridone의 oxygen bases에 대한 hydrogen bonding equilbrium constant는 다른 N-H acid의 같은 bases에 대한 예상처보다 큰 것으로 보였다. Hydrogen bonding association of 2-pyridone to N-methyl-2-pyrrolidone in carbon tetrachloride at 25° was measured( 94N^(-l)). After fixing the complaxation constant, the dunerization constant of 2-pyridone was also calculated from the relationship between the pK_(a), of N-H acids and log K for bases and got the value of kd as 12x10^(5) with the standard deviation of 0.002. it seems that the hydrogen bonding equilibrium constant for 2-pyridone to the oxygen bases is larger than the expected from the other N-H acid to the same base.

고장으로 인한 대구경 계량기의 불감률

박남식,배정용,황경석,김학용,최철식 대한상하수도학회 2003 상하수도학회지 Vol.17 No.2

Leaks and meter under registrations are suspected as primary components of unaccounted-for water in Korea. Roughly, one third of the unaccounted-for water is believed to be due to meter under registration. However, no justification has been given to prove the statistics. In this study the rate of meter under registration due to failures of large-diameter meters is investigated. Replacement records of over 2600 consumer sites are analyzed. The minimum, the maximum and the probabilistic distribution of meter under registration rate are computed.

감자의 조리방법에 따른 물성 변화

이정숙(Lee Jeong Sug),황영정(Hwang Yeong Jeong) 한국조리학회 2003 한국조리학회지 Vol.9 No.2

외래 간호인력 업무활동 수행도와 중요도 분석 : 종합병원·종합전문요양기관 중심으로

황혜영,박정혜,김지수,전인숙,김문실,배경옥,서미숙,양우정,정문영,채지선,홍지연 대한간호행정학회 2007 간호행정학회지 Vol.13 No.1

Purpose: This study focused on analysing the performance and the perception of importance about workload of ambulatory nurses and nurse-aides for quality of nursing. Method: The subjects of this study were 126 ambulatory nurses and 117 nurse-aides in 6 secondary and 4 tertiary hospitals. The method of data collection was used the questionnaire. Result: As a result, First, nurses' activities that the performance score is above 3.0 are reception, guidance, reservation, confirm, checking medical record, operating report, explanation of disease, explanation of examination discuss with medical part, discuss with supporting part, solving patient problem environment management, and paper work. And the other side, those of nurse-aides are reception, guidance, reservation, preparation for clinic, assistant for clinic, preparation for examination, material transfer & receipt, confirm, checking medical record, and arrangement. Second, nurses-aids perceive above 3.0 performance score activities to be important for themselves. Finally, nurses perceive three categories of patient education/counselling, patient advocacy and quality improvement to be more important and higher performance when compared with nurse-aides. Conclusions: Ambulatory nurse's important nursing activities are therapeutic care, Patient education/counselling, patient advocacy, communication, personal management, quality improvement.

Edwardsiellosis의 진단을 위한 정성적 ELISA법

정현도,허민도,김명석,황은아 한국어병학회 1999 한국어병학회지 Vol.12 No.1

어류의 edwardsiellosis를 진단하기 위한 solid phase ELISA법에 대한 최적화 분석을 실시하였다. 부분 정제한 E. tarda Edk-2에 대한 토끼 항혈청을 sodium bicarbonate 완충용액에 50㎍/㎖ 농도로 희석하여 overnight 반응시켜 주었을 때 polystyrene bead 표면의 항체 immobilization이 최적화되었다. 50㎍/㎖의 biotin 표지화 항체와 1:2000으로 희석된 extravidin-peroxidase를 차례로 처리하였을 때 최적의 반응을 나타내었으며 이렇게 최적화시킨 정성적 solid phase ELISA법은 EDTA 추출법으로 조제된 항원에 대해서는 1×10^5 cells/㎖, 열 추출법으로 조제된 항원에 대해서는 5×10^5 cells/㎖의 검출한계를 나타내었다. E. tarda Edk-2에 대한 토끼 항혈청을 이용한 본 연구의 solid phase ELISA법은 우리나라 양어장의 넙치 병어로부터 분리한 여러 한국형 E. tarda 균주와도 높은 교차반응을 나타내었다. 이러한 것은 본 기법을 다른 지역에서 분리한 여러 strain의 E. tarda에 대한 진단을 위하여 본 실험의 항혈청을 사용하여 가능하다는 것을 보여 주었다. Optimization and standardization of solid phase enzyme immunoassay were done for the diagnosis of edwardsiellosis in fish. The analyzed degree of immobilized antibody on surface of solid phase with peroxidase saturation method showed the optimized result by using partially purified 50 ㎍/㎖ of rabbit anti-E. tarda Edk-2 antibody in sodium bicarbonate buffer for overnight incubation to cover the surface of polystyrene beads. Optimized immunoreaction was observed in the treatment of 50 ㎍/㎖ of biotin conjugated antibody followed extravidin-peroxidase diluted 1 : 2,000 in PBS. The detectable concentrations of the this method were 1 × 10^5 cells/㎖ and 5 × 10^5 cells/㎖ expressed as the source of antigen amount for EDTA extraction and heat extraction, respectively. High cross-reaction of solid phase ELISA with the prepared rabbit and-E. tarda Edk-2 was observed against E. tarda strains isolated from flounder suffering from edwardsiellosis in aquatic farms of Korea. It suggested that the potential of this solid phase of ELISA technique is very powerful for the application to different strains of E. tarda isolated in farms of many different areas.

기관지 스텐트 삽입으로 치료한 전폐절제술후 증후군

정성현 ( Jeong Seong Hyeon ),조혜진 ( Jo Hye Jin ),이형노 ( Lee Hyeong No ),이형숙 ( Lee Hyeong Sug ),신승수 ( Sin Seung Su ),오윤정 ( O Yun Jeong ),박광주 ( Park Gwang Ju ),황성철 ( Hwang Seong Cheol ),원제환 ( Won Je Hwan ),박 대한결핵 및 호흡기학회 2002 Tuberculosis and Respiratory Diseases Vol.53 No.3

HBsAg 음성 및 양성 간질환에서 C형 간염바이러스 항체 양성율

황이숙 ( Hwang I Sug ),김영관 ( Kim Yeong Gwan ),김효종 ( Kim Hyo Jong ),김병호 ( Kim Byeong Ho ),장영운 ( Jang Yeong Un ),이정일 ( Lee Jeong Il ),장린 ( Jang Lin ) 대한내과학회 1992 대한내과학회지 Vol.42 No.3

연구배경 : 최근 유전공학적인 진단방법의 발전으로 과거 그 감염원이 확인되지 않았던 비A, 비B형 간염바이러스의 colne과 genome이 확인되었다. Recombinant viral antigen (C100-3)을 이용한 혈중 C형 간염바이러스에 대한 항체검사로 C형간염바이러스는 수혈수 간염 및 산발성 비A, 비B형 간염의 중요한 원인으로 알려져 있으며, 원발성 간암이나 다른 간질환과의 연관성도 보고 되고 있다. 방법 : HBsAg이 음성인 만성 간질환환자에서 anti-HCV양성율을 알아보고, HBsAg이 양성인 만성간질환에서 C형간염바이러스의 동반감염이 어떠한 영향을 미치는지를 알아보고자 C형 간염바이러스항체검사(antiHCV)를 Abbott사의 효소면역측정법으로 검사하였다. 결과 : 1) HBsAg이 음성인 환자 3명중 알콜성간질환환자는 10명이었으며 이중 1명(10%)에서 anti-HCV양성이었고, 만성 비A, 비B형 간질환환자는 27명중 11명 (40.7%)에서 양성으로 검출되었다. 2) 117명의 HBsAg양성인 무증상보균자 및 만성간질환환자중 5명 (4.3%)에서 anti-HCV 양성으로 검출되었고, 이중 무증상보균자는 40명중 1명에서 양성으로 2.5%, 만성활동성간염환자에서는 검출된 환자는 없었으며, 간경변증환자에서는 23명중 1명에서 양성으로 4.3%, 원발성간암환자에서는 33명중 11명에서 양성으로 9%의 양성율을 보였다. 그러나 병의 진행과 anti-HCV 양성율과는 의미있는 상관관계를 찾을 수 없었다. 결론 : 이상의 결과로 우리나라에서도 C형 간염바이러스는 비A, 비B형 간질환을 유발하는 주요원인으로 생각되었으며, 아울러 C형간염바이러스의 동반감염이 HBsAg 양성 만성간질환의 진행에 영향을 주지는 않을 것으로 생각되었으나, 추가연구가 필요할 것으로 사료되었다. Background : A recombinant immunoscreening approach was used to clone and identify the genome of the previously uncharacterized non-A, non-B (NANB) virus. A recombinant viral antigen (C100-3) was used to develop a capture assay for circulating antibody. Data obtained using this assay indicate that this agent, termed hepatitis C virus (HCV) is known to be associated with the development of hepatocellular carcinoma and possibly, other liver diseases. Methods : To evaluate the prevalence of anti-HCV in patient with HBsAg negative and HBsAg positive chronic liver diseases, we tested 189 serum samples using the Abbott enzyme immunoassay method. Besides, we analysed the role of HCV superinfection in the progression of HBsAg positive chronic liver diseases. Results : 1) In 37 patients with HBsAg neative chronic liver diseases, anti-HCV was detected in 1 of 10 alcoholic liver diseases (10%), 11 of 27 chronic NANB liver diseases (40.7%). 2) In 117 patients with HBsAg positive asymptomatic carriers and chronic liver diseases, only 5 cases (4.3%) were positive for anti-HCV. That is, anti-HCV was detected in 1 of 40 asymptomatic carriers (2.5%), none of 21 chronic active hepatitis, 1 of 23 liver cirrhosis (4.3%) and 3 of 33 hepatocellular carcinoma (9.0%). But the prevalence rate of anti-HCV was not significatly different between each chronic liver diseases. Conclusions: HCV may also be one of the most important causative agents of chronic NANB liver diseases in Korea. But the rate of the simultaneous infection of HCV in patients with HBsAg positive chronic liver disease may be very low in Korea and HCV superinfection may not be able to modify the natural course of HBsAg positive chronic liver diseases.

Circulatory endostatin level and risk of cardiovascular events in patients with end-stage renal disease on hemodialysis

( Jin Sug Kim ),( Miji Kim ),( Kyung Hwan Jeong ),( Ju-young Moon ),( Sang Ho Lee ),( Gang Jee Ko ),( Dong-young Lee ),( So Young Lee ),( Yang Gyun Kim ),( Hyeon Seok Hwang ) 대한신장학회 2024 Kidney Research and Clinical Practice Vol.43 No.2

Background: Endostatin is released during extracellular matrix remodeling and is involved in the development of vascular pathology and cardiovascular (CV) disease. However, the role of circulating endostatin as a biomarker of vascular calcification and CV events in patients undergoing hemodialysis (HD) remains unclear. Methods: A total of 372 patients undergoing HD were prospectively recruited. Plasma endostatin levels were measured at baseline, and their associations with circulating mineral bone disease (MBD) biomarkers and abdominal aortic vascular calcification scores were analyzed. The primary endpoint was defined as a composite of CV and cardiac events. Results: Plasma levels of patients in endostatin tertile 3 were significantly associated with low-density lipoprotein cholesterol levels and predialysis systolic blood pressure in multivariate analysis. However, endostatin levels did not correlate with circulating MBD biomarkers or vascular calcification scores. Patients in endostatin tertile 3 had a significantly higher cumulative event rate for the composite of CV events (p = 0.006). Endostatin tertile 3 was also associated with an increased cumulative rate of cardiac events (p = 0.04). In multivariate Cox regression analyses, endostatin tertile 3 was associated with a 4.37-fold risk for composite CV events and a 3.88-fold risk for cardiac events after adjusting for multiple variables. Conclusion: Higher circulating endostatin levels were independently associated with atherosclerotic risk factors but did not correlate with MBD markers or vascular calcification. Higher circulating endostatin levels were associated with a greater risk of composite CV events in patients undergoing HD, and endostatin is a biomarker that helps to determine the high risk of CV events.

PLHC-1세포주의 Comet assay를 이용한 하천 퇴적토의 생태독성평가

박정아 ( Jeong Ah Bak ),황인영 ( In Young Hwang ),백승홍 ( Seung Hong Baek ),김영숙 ( Young Sug Kim ) 한국환경생물학회 2011 환경생물 : 환경생물학회지 Vol.29 No.1

In this study, the Comet assay (evaluation of DNA damage) used the fish hepatocellular carinoma cell, PLHC-1, was tried to the sediment extract obtained from freshwater to understand its applicability as a tool for monitoring sediment toxicity. In parallel, induced EROD (7-ethoxyresorufin-O-deethylase) activity and DNA damage (TEM values) in PLHC-1 cells were measured for establishing the tandem endpoints of the PLHC-1cell test to test the ecotoxicity of sediment. Among several study sites in a small river passed through downtown and industrial park area, one of them, site B, showed a higher level of EROD activity and DNA damage than other sites. It indicates that a tandem endpoints of PLHC-1 cells could be useful tools for assessing the toxicity of sediment. The sensitivity of Comet assay with PLHC-1 cells was a little higher than that with a blood cell of frog tadpoles to the solvent extract of sediment. According to the results, a PLHC-1 cell-Comet assay could be used as a useful tool for evaluating ecotoxicity of the freshwater sediment. In addition, more detailed studies are needed to the contaminated site.

소아, 청소년 아토피 피부염에서의 DPCP 면역요법의 임상적 치료효과

방숙현 ( Bang Sug Hyeon ),최유원 ( Choe Yu Won ),함정희 ( Ham Jeong Hui ),황규광 ( Hwang Gyu Gwang ) 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.1

N/A Background: Atopic dermatitis is the most common chronic skin disease in childhood. Severe atopic dermatitis may require use of topical or systemic corticosteroids. These treatments are prone to several adverse side effects. This paper highlights the impressive effect of diphenylcycloprope-none(DPCP) sensitization in the some cases of childhood and adolescent atopic dermatitis. Objective: The purpose of this study was to examine the effect of DPCP in treating severe atopic dermatitis of children and adolescents. Methods: Twenty eight patients, who had moderate to severe atopic dermatitis, were recruited in the treatment of DPCP weekly for 10 weeks and followed up for another 4 weeks. Severity of atopic dermatitis was measured using SCORAD index. Results: Sixteen of 28 patients had finished the treatment courses. After 10 weeks of therapy with DPCP, the patient`s symptoms were improved and the mean SCORAD index was significantly reduced from 48.9±17.8 to 18.0±9.1 (p<0.01). During the follow-up period, patients could be managed by topical steroid and oral antihistamine and flare-up was no observed. Conclusion: According to our study, DPCP can be an effective, safe and well-tolerated therapy for moderate to severe atopic derm atitis in children and adolescents. We suggest that DPCP may be effective in the treatment of moderate to severe atopic dermatitis of children and adolescents without serious complications. (Korean J Dermatol 2004;42(1):29-36)