Modulation of store-operated calcium entry and nascent adhesion by p21-activated kinase 1

전인숙,김혜련,신은영,김응국,한헌석,홍진태,이학교,송기덕,최중국 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Calcium mobilization is necessary for cell movement during embryonic development, lymphocyte synapse formation, wound healing, and cancer cell metastasis. Depletion of calcium in the lumen of the endoplasmic reticulum using inositol triphosphate (IP3) or thapsigargin (TG) is known to induce oligomerization and cytoskeleton-mediated translocation of stromal interaction molecule 1 (STIM1) to the plasma membrane, where it interacts with the calcium release-activated calcium channel Orai1 to mediate calcium influx; this process is referred to as store-operated calcium entry (SOCE). Furthermore, aberrant STIM1 or SOCE regulation is associated with cancer cell motility and metastasis. The p21-activated kinases (PAKs), which are downstream effectors of GTPases, reportedly regulate cytoskeletal organization, protrusive activity, and cell migration. Although cytoskeletal remodeling apparently contributes to calcium mobilization via SOCE, and vice versa, the mechanisms by which they regulate each other remain unclear. In this study, we aimed to characterize whether PAK1 modulates calcium mobilization and STIM1 localization. Our data demonstrate that PAK1 interacts with STIM1 in vitro and that this interaction was enhanced by treatment with a nascent adhesion inducer, such as phorbol 12,13-dibutyrate (PDBu). Under basal conditions, both proteins appeared to primarily colocalize in the cytosol, whereas treatment with PDBu induced their colocalization to vinculin-positive peripheral adhesions. Downregulation of PAK1 activity via chemical inhibitors or by PAK1 shDNA expression impaired STIM1-mediated calcium mobilization via SOCE. Based on these findings, we propose that PAK1 interacts with STIM1 to regulate calcium mobilization and the formation of cellular adhesions.

겨울 공원 외 1편

전인숙 숙명문학인회 2010 숙명문학 Vol.- No.1

탐구력 신장을 위한 기능평가에 관한 연구

전인숙 晋州敎育大學校 科學敎育硏究所 1983 科學敎育硏究 Vol.9 No.-

Characterization of two antimicrobial peptides identified from a random peptide library and expressed in the methylotrophic yeast pichia pastoris

전인숙,송기덕,오재돈,최중국 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.4

Antimicrobial peptides (AMPs) are an important component of innate defense mechanisms with broad-spectrum activities against various pathogenic microorganisms, including Gram-positive and Gram-negative bacteria, fungi, and viruses. Antibiotic resistance has become a pervasive and global health burden, resulting in the immediate need to develop a new class of antibiotic substances. We screened a 16-mer random peptide library using the yeast two-hybrid system with Beclin 1 as bait and found that two 16-mer peptides (named P4 and P30) appeared to interact with Beclin1 in the β-gal assay. The two candidate cDNAs were introduced into the yeast secretory system of Pichia pastoris and their expression induced in the presence of methanol. Spectrophotometric analysis and Disc clear zone assay using the supernatant of the yeast growth media showed that both of the two peptides had strong activities against Staphylococcus aureus, MRSA (methicillin resistance Staphylococcus aureus), MRSA2242, and MRSA-2250, but no effect on commensal Lactobacillus strains. PCR analysis of the genomic DNA of transformed Pichia pastoris using AOX1 primers revealed that the two cDNAs were integrated into the genome at the AOX1 locus. Our result suggests that these peptides could be developed as a useful alternative to classic chemical antibiotics.

편도 절제술을 받은 환아의 전신마취 회복 중에 발생한 중증 심실 빈맥 및 심근염 의증

전인숙,이종현,김상백,이동기,배상철 순천향대학교 순천향의학연구소 2011 Journal of Soonchunhyang Medical Science Vol.17 No.2

A 10-year-old female child was underwent the tonsillectomy and adenoidectomy. She was relieved from the upper respiratory tract infection about 1 week before the operation. Her heart rate was recorded 100 to 110 per minute at the preanesthetic period, and then increased to about 140 per minute during the operation. We speculated that tacchycardia resulted from the inhalation anesthesia of sevoflurane as other common cases, so we didn’t consider it as a serious problem. But the taccycardia was not relieved after the termination of anesthesia, and after the extubation, it was severely and rapidly aggravated to the ventricular tacchycardia with the circulatory collapse during the emergence period. After the rapid defibrillation and the chest compression, her resuscitation was successfully finished. We suspected her event was derived from the childhood cardiomyopathy, especially the viral myocarditis. So we reviewed viral myocarditis and focused a new aspect of childhood cardiac disease and screening.

Role of RS-1 derivatives in homology-directed repair at the human genome ATG5 locus

전인숙,Jae-Cheon Shin,Seung Ryul Kim,Kwan Sik Park,Hyun Jung Yoo,이광열,이학교,Joong-Kook Choi 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.6

Genome editing is a useful tool in basic andclinical research. Among the several approaches used ingenome editing, the CRISPR–Cas9 system using clusteredregularly interspaced short palindromic repeats (CRISPR)and CRISPR-associated protein 9 (Cas9) along with a guideRNA has been developed recently. The CRISPR/Cas9 systeminduces site-specific double-stranded DNA breaks,which result in DNA repair via non-homologous end joining(NHEJ) or homology-directed repair (HDR). However,HDR effi ciency is lower than that of NHEJ and accordinglyposes a challenge in genome modifi cation studies. Severalchemical compounds including RS-1 have been shown toenhance the HDR knock-in process by two- to six-fold in HEK 293 cells and rabbit embryos. Based on this fi nding,we developed an antibiotic resistance system to screen RS-1chemical derivatives, which may promote effi cient HDR. Inthis study, we report several chemical compounds with highknock-in effi ciency at the ATG5 gene locus, using HeLacell-based assays.

단기 해외자원봉사 활동이 대학생의 글로벌 리더십에 미치는 효과

전인숙,김경미,황혜영 사단법인 인문사회과학기술융합학회 2017 예술인문사회융합멀티미디어논문지 Vol.7 No.6

본 연구는 세계화와 글로벌 시대에 따라 많은 대학에서 시행되고 있는 단기 해외자원봉사활동이 대학생의 글로벌 리더십에 미치는 효과를 파악하기 위해 시도된 단일군 사전·사후 비교조사 연구이다. 11박12일 간의 필리핀 단기 해외자원봉사에 참여한 대학생 46명을 대상하였다. 연구결과 국제교류 참여경험이 있는 대상자들이 인성영역에서 '명목적 참여’(평균 4.40±.40점)가 가장 높았고 통계적으로 유의한 차이가 있었다(p=.042). 단기 해외자원봉사 활동 전․후를 글로벌 리더십의 차이는 단기 해외자원 봉사활동 후 ‘호기심’,‘이원성’,‘인성’,‘통찰력’영역에서 증가를 보였고, 글로벌 리더십 전체 점수도 평균 3.32±.50점에서 평균 3.74±.38점으로 유의한 증가(p<.01)를 보임으로써 단기 해외자원봉사활동이 글로벌 리더십 향상에 효과가 있음을 확인 할 수 있었다. 본 연구를 통해 해외자원 봉사활동이 학생들의 인성과 통찰력의 능력을 증진시킴으로 향후 다양한 기관들과의 연계를 통한 해외자원봉사 프로그램이 개발되어야 할 것이다. This research is to understand the effect of global leadership of university students due to short term foreign volunteer activity. It is 46 participant with experience in foreign volunteer activity in the 12 day short term foreign volunteer activity in the Philippines. The research is a One-Group Pretest-Posttest Disign. The results out of the character field of experienced participants, nominal participation showed highest (4.40±.40) and showed statistical significance(p=.042). The experienced showed a increase in fields of character, duality and insight compared the inexperienced(p<.01). Also the total average of global leadership had a increase from Mean 3.32±.50 to Mean 3.74±.38 showing improvement of global(p<.01) leadership through short term foreign volunteer activity. Through this research, Foreign volunteer activity of University Students is showing improvement of global leadership of university students and programs that allow dominant participation of students should be developed.

쓰기 과제의 수사적 요소에 대한 필자의 인식 연구

전인숙 한국초등국어교육학회 2011 한국초등국어교육 Vol.45 No.-

By investigation of perception of writer's writing assignment upon rhetorical component, this study assist teacher's writing assignment design for communicative writing of elementary school student. The rhetorical components of writing assignment are topic, audience,purpose. Based on rhetorical components, three assignments (① topic, ②topic and audience, ③ topic, audience and purpose) are designed. The writers wrote one of three assignments per a week. After writing, writer's perception of writing assignment upon rhetorical component was investigated. According to the results, before writing, the writers perceived difficult assignment when rhetorical components were specificity, after writing, they perceived easer than before writing. Upon Assignment①, Audience was teacher. Upon Assignment② and Assignment ③, Audience was graduator,student in Seoul and teacher. writers perceived teacher as audience. The Processes of writing differed to assignments. The more rhetorical components are specificity, the writers required the more planning time. Because the audience and purpose are necessary for communicative writing, the teachers have to design writing assignment by rhetorical components' specification. 이 연구는 수사적 요소를 달리 하여 쓰기 과제를 제시했을 때 학생 필자들이 쓰기과제를 어떻게 인식하는가를 탐색함으로써 교사가 초등학생 필자로 하여금 의사소통적 쓰기를 할 수 있도록 쓰기 과제를 설계하는 데 도움을 제공하는 것을 목적으로한다. 이 연구에서는 쓰기 과제의 수사적 요소를 주제, 독자, 목적으로 규정하였다. 이에따라 ①주제, ②주제와 독자, ③주제와 독자와 목적이 제시된 쓰기 과제를 설계하였다. 각 과제에 대해 필자들은 일주일마다 한 차례씩 모두 세 차례에 걸쳐 글을 썼다. 글을 쓰고 난 직후 각 과제에 대한 필자의 인식을 설문 조사하였다. 그 결과 필자들은 글을 쓰기 전에는 수사적 요소가 구체적인 과제일수록 어려운과제로 인식하였고, 독자 요소가 명시적으로 제시된 과제에서는 과제에 제시된 독자를 인식하고 글을 쓰는 경향을 보였지만 교사를 독자로 인식하는 경우도 상당히 높은비중을 차지하였다. 과제를 해결하는 과정에서 거친 사고 과정에 대한 질문에서는수사적 요소가 구체적일수록 글을 쓰기 전에 계획하기에 많은 시간을 할애하였으며쓰는 도중에서도생각하기 위해 멈추는 횟수가 많아졌다. 필자 수준에 따라서는 상수준 필자는 수사적 요소가 구체적인 과제를 더 선호하는 반면 하 수준 필자는 수사적 요소가 구체적인 과제를 선호하지 않았다. 과제①을 선호한 필자의 경우 그 이유를 쓸 내용이 많거나 쉬워서라고 응답하였고 과제②를 선호하는 필자 대부분은 읽을독자가 있어서라고 응답하였다. 과제③을 선호한 필자는 독자와 글을 쓰는 목적이쓰기 흥미를 자극하였다고 반응하였다. 이 연구는 초등학생 필자의 경우 쓰기 과제에서 수사적 요소(주제, 독자, 목적)를구체적으로 제시하면 이를 인식하고 글을 쓰려고 시도하지만 주제만 제시한 과제에서 필자 스스로 수사적 요소를 구체화하여 글을 쓰는 데 아직 미숙하다는 것을 보여준다. 수사적 요소를 스스로 구체화하지 못한다는 것은 독자나 목적을 구체적으로상정하지 못하여 결과적으로 의사소통적 쓰기가 이루어지기 어렵다. 따라서 교사는초등학생들이 의사소통적 쓰기를 할 수 있도록 쓰기 과제를 설계할 때 쓰기 주제외에 독자와 목적 요소를 구체화하여 제시해 줄 필요가 있음을 시사한다.

고등학생의 비만도(BMI)에 따른 식습관, 영양지식과 식이섭취 태도와의 관계 연구

전인숙,황혜영 사단법인 인문사회과학기술융합학회 2019 예술인문사회융합멀티미디어논문지 Vol.9 No.9

This research is based on analysis of the relationship between the BMI, eating habits, nutritional knowledge and dietary attitude. This study was implemented under explanation of purpose and agreement of a total of 207 students attending in S high school of D city. The research results indicate that 25(12.1%) students are underweight, 117(56.5%) students are normal weight and 35(16.9%) students are overweight. General characteristics implied that high percentage of obese students were 3rd year and male seniors. Dietary habits implied that high percentage of obese students were dietary habits having snacks once in 3-4 days. Nutritional knowledge scores of obese students were lower compared to others that were underweight normal or overweight and dietary attitude resulted higher in normal and slightly overweight students than underweight of obese students. Gender of the student and frequency of having snacks showed influence in BMI. Based on these results this research will be used as reference in order to maintain a healthy condition, establish values of organized dietary habits and to learn weight control methods and help improve the diet. 본 연구는 고등학교 학생을 대상으로 BMI와 식습관, 영양지식과 식이섭취 태도와의 관계를 알아보고자 시도하였다. 본 연구는 D도시에 위치한 S고등학교 재학중인 학생을 대상으로 연구 목적을 설명하고 동의하에 실시하였으며, 총 207부를 최종분석 하였다. 연구결과 비만도는 저체중군이 25명(12,1%), 정상체중군이 117명(56.5%), 비만군이 35명(16.9%)로 나타났다. 일반적 특성에서 학년은 3학년 학생이 성별은 남학생이 비만군으로 유의하게 높게 나타났으며, 식습관에서는 간식섭취 횟수가 3~4일에 1회 섭취하는 경우가 비만군으로 유의하게 높게 나타났다. 영양지식 점수는 비만군이 저체중, 정상체중, 과체중군보다 점수가 낮게 나타났으며, 식이섭취 태도는 정상체중군과 과체중군이 저체중군과 비만군보다 긍정적인 식이섭취 태도를 하고 있는 것으로 나타났다. BMI에 영향을 미치는 요인으로는 성별과 간식섭취 횟수가 유의한 영향을 미쳤다. 연구결과를 토대로 청소년들의 건강한 신체를 유지하고, 식습관 및 체중조절 방법에 대한 가치관을 정립하고 식생활 향상에 도움이 되는 기초 자료가 될 것으로 사료된다.

그리운 날

전인숙 숙명문학인회 2013 숙명문학 Vol.- No.2