丹參飮 煎湯液이 心筋細胞 搏動數에 미치는 影響

손창식,권강범,김상범,이호승,서은아,이호섭,류도곤 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.2

In order to elucidate toxic mechanism of myocardial damage and protective effect of herbal extract, Dansamyeum (丹參飮) against cytotoxic effect of xanthine oxidase/hypoxanthine(XO/HX) and cardioprotective effect of Dansamyeum (丹參飮) were examined by MTT assay and heart beating rate after cultured myocardial cells derived from neonatal mouse were treated with various concentrations of XO/HX, a free radical. The results of these experiments were fellows : XO/HX, a oxygen radical, decreased the survial rate on MTT assay in cultured myocardial cells. Dansmyeum(丹參欲) water extract have efficacy of increasing heart beating rate decreased by XO/HX in cultured myocardial cells. Radix Salviae Miltiorrhizae and Lignum Santali Album water extract have efficacy of increasing heart beating rate decreased by XO/HX in cultured myocardial cells. From above the results, it is suggested that XO/HX showed toxic effect in cultured myocardial cell derived from neonatal mouse and herbal extract such as Dansamyeum(丹參飮) is very effective in the prevetion of XO/HX cardiotoxicity. Key ward : Dansamyeum(丹參飮), Xanthine Oxidase/Hypoxanthine(XO/HX), myocardial Cell, Radix Salviae Miltiorrhizae, Lignum Santali Album, MTT, beating rate

Purification and Characterization of Two Extracellular Proteases from Oligotropha carboxydovorans DSM 1227

Kang, Beom-Sik,Jeon, Sang-Jun,Kim, Young-Min The Microbiological Society of Korea 1999 The journal of microbiology Vol.37 No.1

Two extracellular proteases, EP I and EP II, from cells of Oligotropha carboxydovorans (formerly Pseudomonas carboxydovorans) DSM 1227 grown in nutrient broth were purified to greater than 95% homogeneity in five steps using azocasein as a substrate. The final specific activities of EPs I and II were 214.9 and 667.4 units per mg of protein. The molecular weights of native EPs I and II were determined to be 23,000. Sodium dodecyl sulfate-gel electrophoresis revealed the two enzymes to be monomers. The enzymes were found to be serine-type proteases. The activity of EP I was stimulated by Ca2+, Mg2+, and Ba2+, but that of EP II was not. The enzymes were completely inhibited by Fe2+, Hg2+, Co2+, Zn2+, and Cd2+. EDTA and EGTA exhibited a strong inhibitory effect on EP I. The optimal pH for the two enzymes was pH 9.0. The optimal temperatures for EP I and II were 60 and 50$^{\circ}C$, respectively. The enzymes were stable under alkaline conditions. The thermal stability of EP I was higher than that of EP II. Cell-free extracts did not inhibit the purified enzymes. The enzymes were active on casein, azocasein, azocoll, and carbon monoxide dehydrogenase, but weakly active with bovine serum albumin.

단백질 복합체를 위한 접촉 영역의 3차원 가시화

강범식 ( Beom Sik Kang ),김구진 ( Ku Jin Kim ),김유경 ( Yu Kyeong Kim ) 한국정보처리학회 2013 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.2 No.12

본 논문에서는 두 개의 단백질 분자가 결합하여 복합체를 구성할 때, 두 분자 간의 접촉 영역을 발견하여 3차원 공간에서 가시화하는 방법을 제시한다. 두 분자 간의 접촉 영역은 기하학적인 측면에서 서로 상보성을 보이며, 상보적인 결합의 크기를 나타내기 위한 방법으로 접촉 영 역의 면적을 계산하는 방법이 주로 사용되어 왔다. 접촉 영역의 면적을 수치화한 결과와 접촉 영역의 단순 표시는 서로 다른 접촉 영역에 대해 상대적인 결합 강도를 비교하기에는 적합하지만, 접촉 영역이 가진 기하학적인 특성을 분석하기에는 부적합하다. 본 논문에서는 접촉 영역에서 상보성을 표시하기 위해 상대 분자와의 거리 정보를 가시화하는 방법을 제시한다. In this paper, we present a method to visualize the contact region between two molecules in a protein complex in a threedimensional space. The contact region of two molecules shows compatibility in geometric aspects. Usually, the computation of the area of contact region has been used to show the strength of compatibility. The numerical value and simple drawing of contact region would be useful for comparing the relative strength of different contacts, but it is not appropriate for analysing the geometric characteristics of the contact region. In this paper, we present a method to show the compatibility between two molecules by visualizing the distance information between them.

Genetic and Environmental Variation of First Pod Height in Soybean [Glycine max (L.) Merr.]

( Beom-kyu Kang ),( Hyun-tae Kim ),( Man-soo Choi ),( Seong-chul Koo ),( Jeong-hyun Seo ),( Hong-sik Kim ),( Sang-ouk Shin ),( Hong-tae Yun ),( In-seok Oh ),( Krishnanand P. Kulkarni ),( Jeong-dong Le 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.1

First pod height (FPH) is an agronomic trait for the mechanical harvesting of soybeans with combines. The seed loss could be minimized, if the FPH is higher than the height of the cutter bar in combines. Hence, developing soybeans with high FPH has become one of important breeding goals in current crop improvement programs. The objective of this study was to evaluate genetic and environmental variation of FPH in soybean and to analyze the effect of ratio of FPH to plant height (PH) on seed yield. Four genotypes were evaluated across six different environments to analyze environmental variation of agronomic traits including FPH. Three F₂ populations were evaluated to analyze genetic variation and relationship between the ratio of FPH to PH and seed yield. The main effects of planting distance, genotype and seeding date were significant for FPH, but FPH is affected more by genetic factors than by environmental factors. The mean heritability value of FPH was 66% across three F₂ populations. Seed yield was found to reduce with increase in the FPH/PH ratio. In conclusion, genetic factors have effect more than environments to the variation of FPH. While FPH is higher than cutting height, the smaller ratio can minimize seed yield decrease.

Catalytic Rapid Pyrolysis of<i>Quercus variabilis</i>over Nanoporous Catalysts

Kang, Hyeon Koo,Lee, In-Gu,Lee, Kyong-Hwan,Kim, Beom-Sik,Jo, Tae Su,Chea, Kwang-Seok,Park, Sung Hoon,Jung, Sang-Chul,Park, Young-Kwon Hindawi Limited 2015 Journal of nanomaterials Vol.2015 No.-

<P>Catalytic rapid pyrolysis of<I>Quercus variabilis</I>, a Korean native tree species, was carried out using Py-GC/MS. Mesoporous MFI, which has both nanopores and micropores, and three nanoporous materials, Al-MCM-41, Al-SBA-15, and<I>γ</I>-Al2O3, were used as the catalyst. The acid sites of mesoporous MFI were strong Brønsted acid sites, whereas those of nanoporous materials were mostly weak acid sites. The composition of the product bio-oil varied greatly depending on the acid characteristics of the catalyst used. Phenolics were the most abundant species in the bio-oil, followed by acids and furanics, obtained over Al-MCM-41 or Al-SBA-15 with weak acid sites, whereas aromatics were the most abundant species produced over mesoporous MFI with strong acid sites, followed by phenolics. Aromatics, phenolics, and furanics are all important chemicals contributing to the improvement of bio-oil quality.</P>

Effect of Dodecane on the Surface Structure and the Electronic Properties of Pentacene on Modified Si (001)

Beom-sik Kim,Hee Jae Kang,Soonjoo Seo,Nam Seok Park 한국진공학회(ASCT) 2016 Applied Science and Convergence Technology Vol.25 No.2

The structural and the electronic properties of pentacene on modified Si (001) were investigated using scanning tunneling microscopy (STM), atomic force microscopy (AFM) and ultraviolet photoelectron spectroscopy (UPS). Dodecane was used to modify Si (001) substrates and then pentacene was deposited on dodecane/Si (001). Our STM results show a uniform distribution of aggregated dodecane molecules all over the clean Si (001). The surface structure of pentacene on dodecaene/Si (001) examined by AFM is analogous to that of pentacene on SiO2. The UPS data showed that the work function of pentacene on clean Si (001) and pentacene on modified Si (001) with dodecane was 6.41 and 5.57 eV, respectively. Our results prove that dodecane results in the work function difference between pentacene on clean Si (001) and pentacene on dodecane/Si (001).

Early maturing, pod-shattering resistant, and large seed soybean cultivar Pungoll'

Beom-Kyu Kang,Jeong-Hyun Seo,Man-Soo Choi,Jun-Hoi Kim,Su-Vin Heo,Jee-Yeon Ko,Nam-Geol Kim,Hong-Sik Kim,Yeong-Min Jo,Chang-Heui Son,Choon-Song Kim 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

Variation of Protein, Lipid, Fatty Acid and Sugar Contents of Soybean Seeds in Drought Condition

Beom Kyu Kang,Sung Cheol Koo,Man Soo Choi,Jeong Hyeon Seo,Hyun Tae Kim,Hong Sik Kim,Sang Ouk Shin,Chang Hwan Park,Zhang Xiong Liu,Do Yeon Kwak 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

위의 유암종과 선종의 병발 1례

장재식,강혁주,이중현,서영범,윤병구,김용섭,이구,서정일,양창헌,이창우,김정란,윤환중 東國大學校醫學硏究所 2000 東國醫學 Vol.7 No.-

유암종은 전신에 퍼져 잇는 장크롬친화성 세포에서 기원하는데 위의 유암종은 비교적 드문 질환으로 모든 유암종의 1.9~2.2%에 해당하며 위에 발생하는 종양의 1% 미만에서 보고되고 있다. 유암종은 일반적으로 증상이 비특이적이고 면역조직화학검사에 의해서 진단되기 때문에 과거에는 드물게 발견되었으나 최근 내시경 기기 및 술기의 발전과 검사 빈도의 증가 및 면역조직화학검사의 발달로 진단율이 증가 추세에 있으며, 드물지만 유암종에서 선암과 공존하는 예들이 보고되고 있다. 저자들은 심와부 동통을 주소로 내원한 70세 남자 환자의 상부위장관내시경 검사에서 위체상부의 대만곡에서 0.4×0.4 cm 크기의 중간 함몰을 가진 Yamada 1형의 용종과 함께 전정부의 전벽에서 0.8×0.5 cm 크기의 융기된 점막 소견을 보여 조직학적으로 각각 위유암종 및 위선종임을 확진한 후 내시경적 용종 절제술로 치료한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Carcinoid tumors are arisen from enterochromaffin cells, which are scattered throughout the body. These tumors are composed of variable numbers of cells that contain endocrine granules in their cytoplasm, which can be identified by immunohistochemistry and electron microscopy. Gastric carcinoid tumors are relatively rare tumor, the reported incidence being 1.9~2.2% of all carcinoid tumors and less than 1% of all gastric tumors. Sometimes, composite carcinoid tumors and adenocarcinoma is reported. We experienced a case of the coincidence of carcinoid tumor and adenoma at the other site of the stomach in a 70 year old male patient. Gastrofiberscope showed 0.4×0.4 cm sized Yamada type I polyp with central depression on the greater curvature of the proximal body and 0.8×0.5 cm sized focal slightly elevated mucosa on the anterior wall of the antrum. The endoscopic biopsy specimens revealed carcinoid tumor and tubular adenoma with moderate atypism, respectively. The carcinoid tumor and adenoma were removed by endoscopic polypectomy. According to the follow-up gastrofiberoscopy after 7 month later, both lesions were nearly normal mucosa except whitish atrophic change after polypectomy.