서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Comparison of Histologic Core Portions Acquired from a Core Biopsy Needle and a Conventional Needle in Solid Mass Lesions: A Prospective Randomized Trial

( Ban Seok Lee ),( Chang-min Cho ),( Min Kyu Jung ),( Jung Sik Jang ),( Han Ik Bae ) 대한간학회 2017 Gut and Liver Vol.11 No.4

Background/Aims: The superiority of endoscopic ultrasound- guided fine needle biopsy (EUS-FNB) over EUS-guided fine needle aspiration (EUS-FNA) remains controversial. Given the lack of studies analyzing histologic specimens acquired from EUS-FNB or EUS-FNA, we compared the proportion of the histologic core obtained from both techniques. Methods: A total of 58 consecutive patients with solid mass lesions were enrolled and randomly assigned to the EUSFNA or EUS-FNB groups. The opposite needle was used after the failure of core tissue acquisition using the initial needle with up to three passes. Using computerized analyses of the scanned histologic slide, the overall area and the area of the histologic core portion in specimens obtained by the two techniques were compared. Results: No significant differences were identified between the two groups with respect to demographic and clinical characteristics. Fewer needle passes were required to obtain core specimens in the FNB group (p<0.001). There were no differences in the proportion of histologic core (11.8%±19.5% vs 8.0%±11.1%, p=0.376) or in the diagnostic accuracy (80.6% vs 81.5%, p=0.935) between two groups. Conclusions: The proportion of histologic core and the diagnostic accuracy were comparable between the FNB and FNA groups. However, fewer needle passes were required to establish an accurate diagnosis in EUS-FNB. (Gut Liver 2017;11:559-566)

The Korean Society of Gastroenterology& SLDDS 2041 : Slide Session ; K-BP-31 : Pancreatobiliary ; Neutrophil-Lymphocyte Ratio Predicts Survival in Patients with Advanced Cholangiocarcinoma on Chemotherapy

( Ban Seok Lee ),( Sang Hyub Lee ),( Dong Kee Jang ),( Kwang Hyun Chung ),( Ji Kon Ryu ),( Yong Tae Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Blood neutrophil-to-lymphocyte ratio (NLR) has been reported to bea prognostic marker in several kinds of cancers. However, no study investigated the prognostic role of NLR in patients with advanced cholangiocarcinoma on chemotherapy. Methods: A total of 221 patients with pathologically-confirmed locally advanced or metastatic cholangiocarcinoma receiving fi rst-line palliative chemotherapy were enrolled. Associations between baseline clinical and laboratory variables, including CA 19-9, CEA, NLR, and platelet-lymphocyte ratio (PLR) and survival were investigated. Optimal cut-points of CA19-9, CEA, NLR, and PLR were determined by martingale residual analyses. Cox-regression analysis was used for multivariable survival analyses. Results: Best cut-point of NLR for the prediction of survival was 5 in the martingale residual plot. Median overall survival (OS) and progression-free survival (PFS) in patients with NLR = 5 were 11 and 7 months whereas 7 and 2 months in patient with NLR >5 (P= 0.001). In the multivariable analysis, signifi cant adverse prognostic factors for OS were tumor location (intra;perihilar;distal, HR=1;1.4;1.9, respectively) and NLR> 5 (HR=1.84). CEA > 20 ng/ml and NLR > 5 were associated with worse PFS (HR 1.49 and 1.82) whereas gemcitabine-based chemotherapy predicted better PFS (HR 0.56). Among 50 patients with initial NLR > 5, 33 patients had NLR = 5 after 2 cycles of chemotherapy, and they had signifi cantly better survival than the others (HR 0.48, P= 0.016). Conclusions: NLR independently predict survival in patients with advanced cholangiocarcinoma undergoing chemotherapy. Considering cost-effectiveness and easy availability, it may be the useful biomarker for the prognosis prediction.

T Cell-Specific siRNA Delivery Using Antibody-Conjugated Chitosan Nanoparticles

Lee, Jangwook,Yun, Kyoung-Soo,Choi, Chang Seon,Shin, Seung-Hwa,Ban, Hong-Seok,Rhim, Taiyoun,Lee, Sang Kyung,Lee, Kuen Yong American Chemical Society 2012 Bioconjugate chemistry Vol.23 No.6

<P>The intracellular delivery of small interfering RNA (siRNA) plays a key role in RNA interference (RNAi) and provides an emerging technique to treat various diseases, including infectious diseases. Chitosan has frequently been used in gene delivery applications, including siRNA delivery. However, studies regarding the modification of chitosan with antibodies specifically targeting T cells are lacking. We hypothesized that chitosan nanoparticles modified with T cell-specific antibodies would be useful for delivering siRNA to T cells. CD7-specific single-chain antibody (scFvCD7) was chemically conjugated to chitosan by carbodiimide chemistry, and nanoparticles were prepared by a complex coacervation method in the presence of siRNA. The mean diameter and zeta potential of the scFvCD7-chitosan/siRNA nanoparticles were approximately 320 nm and +17 mV, respectively, and were not significantly influenced by the coupling of antibody to chitosan. The cellular association of antibody-conjugated nanoparticles to CD4+ T cell lines as well as gene silencing efficiency in the cells was significantly improved compared to nonmodified chitosan nanoparticles. This approach to introducing T cell-specific antibody to chitosan nanoparticles may find useful applications for the treatment of various infectious diseases.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bcches/2012/bcches.2012.23.issue-6/bc2006219/production/images/medium/bc-2011-006219_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bc2006219'>ACS Electronic Supporting Info</A></P>

The Korean Society of Gastroenterology& SLDDS 2038 : Slide Session ; K-BP-28 : Pancreatobiliary ; Prognostic Value of CA 19-9 Kinetics during Gemcitabine-Based Chemotherapy in Patients with Advanced Cholangiocarcinoma

( Ban Seok Lee ),( Sang Hyub Lee ),( Dong Kee Jang ),( Kwang Hyun Chung ),( Ji Kon Ryu ),( Yong Tae Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: CA 19-9 and CEA were reported to be prognostic factors in various malignant diseases, but no studies have investigated the prognostic role of their kinetics during chemotherapy in patients with advanced cholangiocarcinoma. Methods: A total of 223 patients with inoperable cholangiocarcinoma received gemcitabine- based chemotherapy as a fi rst line regimen. Of the patients, 179 had pre-and post-treatment CEA and CA19-9 values. Baseline, pre-, and post-treatment (after 2 cycles of chemotherapy) values of those markers were checked, and survival was compared according to various cutting points of those measurements. Results: Patients with a decrease of = 50% in CA 19-9 level had better survival than the others (16.0 vs. 9.0 mon). However, CEA decline did not predict survival gain. Signifi cant prognostic factors in multivariable analysis included CA 19-9 > 1000U/ ml (HR 1.7), = 50% decline in CA 19-9 level during chemotherapy (HR 0.49), and tumor location (intra;perihilar;distal, HR=1;1.3;1.9, respectively). Subgroup analysis was conducted in 102 patients with baseline CA 19-9 > 37U/ml and bilirubin = 2mg/dL. CA 19-9 decline = 50% was also the only predictor for survival. For the validation of the cut-off point (50%) of CA19-9 decline, receiver operating characteristic analysiswas conducted to predict survival = 11 month (median value in this cohort). A value of 51.6% was the optimal cut-off decline point in CA 19-9 level, and decline of 50% had positive and negative predictive value of 80.7% and 62.0%, respectively. Conclusions: CA19-9 but not CEA kinetics serves as a predictor of better survival in patients with advaced cholangiocarcinoma on gemcitabine-based chemotherapy, and a = 50% decline in CA 19-9 level after 2 cycles of chemitherapy may have clinical utility as a early indicator of better response to gemcitabine-based chemotherapy.

Risk factors of organ failure in cholangitis with bacteriobilia.

Lee, Jae Min,Lee, Sang Hyub,Chung, Kwang Hyun,Park, Jin Myung,Lee, Ban Seok,Paik, Woo Hyun,Park, Joo Kyung,Ryu, Ji Kon,Kim, Yong-Tae WJG Press 2015 WORLD JOURNAL OF GASTROENTEROLOGY Vol.21 No.24

<P>To identify the risk factors for organ failure (OF) in cholangitis with bacteriobilia.</P>

Evaluation of<i>phoP</i>and<i>rpoS</i>mutants of<i>Salmonella enterica</i>serovar Typhi as attenuated typhoid vaccine candidates: virulence and protective immune responses in intranasally immunized mice

Lee, Hui-Young,Cho, Sun-A,Lee, In-Soo,Park, Jong-Hwan,Seok, Seung-Hyeok,Baek, Min-Won,Kim, Dong-Jae,Lee, Seok-Ho,Hur, Sook-Jin,Ban, Sang-Ja,Lee, Yoo-Kyoung,Han, Yang-Keum,Cho, Young-Keun,Park, Jae-Hak Oxford University Press 2007 FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY Vol.51 No.2

<P>The attenuation and immunoenhancing effects of rpoS and phoPSalmonella enterica serovar strain Typhi (Salmonella typhi) mutants have not been compared. Here, three S. typhi deletion mutants (phoP, rpoS, and rpoS-phoP double mutant) are constructed and these mutants are characterized with respect to invasiveness, virulence, and protective immune response compared with wild-type Ty2. It was found that phoP and phoP-rpoS deletion mutants are less invasive to HT-29 cells than the wild-type Ty2 and the rpoS single-deleted strain. The LD(50) of immunized mice was higher for phoP than for rpoS mutants, and the highest for the phoP-rpoS double mutant. In addition, all S. typhi mutants showed an increase in the specific serum IgG levels and T-cell-mediated immunity, and showed equal protection abilities against a wild-type Ty2 challenge after two rounds of immunization in BALB/c mice. It is concluded that phoP genes appear to play a more important role than rpoS genes in both cellular invasion and virulence of S. typhi, but not in immunogenicity in mice. Furthermore, the data indicate that the phoP-rpoS double mutant may show promise as a candidate for an attenuated typhoid vaccine.</P>

대학원 교육의 질 제고를 위한 체제적 분석 연구

반상진(Ban Sang-Jin),신현석(Shin Hyun-Seok),이석열(Lee Seok-Yeul),이은구(Lee Eun-Ku),김경춘(Kim Gyum-Hoon),민병제(Min Byung Jae) 한국교육행정학회 2004 敎育行政學硏究 Vol.22 No.2

이 연구의 목적은 대학원 교육의 질 개선 방안을 모색하기 위하여 수행되었다. 연구의 목적을 달성하기 위하여 대학원 교육의 질적 수준에 대한 구체적이고 실증적인 방안에 대해서 교수들을 대상으로 '대학원 교육의 질 개선에 대한 의견'을 조사하였다. 이를 위해 5개 권역의 대학원 규모를 고려한 15개 대학의 교수750명을 대상으로 설문지를 배부했고, 이중에서 200부를 결과분석에 사용하였다. 주요 내용은 ①대학원 입학제도 방향, ②학사운영, ③교육과정, ④졸업시험 및 논문지도에 대한 것이었고, 통계처리는 계열별 차이를 검증하기 위해 분산분석(ANOVA)을 활용하였다. 이 연구의 결과를 바탕으로 국내 대학원이 국제경쟁력을 갖추고, 사회적·국제적 공신력을 확보하기 위한 자율 혁신 노력에 정책적 시사점을 제공해줄 것으로 기대한다. This study reviewed the variations affecting the quaJity in graduate school and provided detailed policy suggestions for graduate schools in Korea to successfully enhancing quality in education and research based on professors' opinion. Surveys were mailed to 750 subjects in considering the region and scale of graduate schools and the response rate was 27.5%.<br/> The positive policies for enhancing quality in graduate school management system are as followings. First, admission policy ought to be changed to enter qualified and potential students by expanding open enrollment and improving selection process applied various criteria for admission.<br/> Second, the system of an academic degree can be flexibly reformed by policies such as integration of bachelor and master course, and master and doctoral course, expanding cross registration system, and provision of student consumer oriented academic degree course, etc..<br/> Third, curriculum for graduate students can be reorganized to meet the social demands, by introducing accrediting system be approved the collaboration university and enterprise. It can be considered that students opinion are reflected in curriculum organization process.<br/> Fourth, the quality management in graduation process ought to be enforced, by strictly controlling graduation exam and the examination of a thesis.<br/> Finally, we expect that the major policy suggestions would help change culture of graduate schools and enhance quality in the level of education and research.

플래시 SSD의 내부 병렬성을 이용한 배열 기반 DBMS의 스캔 성능 향상

여반석(Ban-Seok Yeo),이창주(ChangJu Lee),이영구(Young-Koo Lee) 한국정보과학회 2016 한국정보과학회 학술발표논문집 Vol.2016 No.12

임신성 유방암과 40세 미만의 유방암과 비교

양반석 ( Ban Seok Yang ),박세호 ( Se Ho Park ),이소희 ( So Hee Lee ),박형석 ( Hyung Seok Park ),황혜원 ( Hye Won Hwang ),이준상 ( Jun Sang Lee ),고시몬 ( Si Mon Ko ),김승일 ( Seung Il Kim ),박병우 ( Byeong Woo Park ) 대한임상종양학회 2011 Korean Journal of Clinical Oncology Vol.7 No.2

목적: 본 연구는 임신성 유방암의 임상병리학적 특징과 예후를 조사하여 임신이 임신성 유방암에 미치는 영향을 평가하고자 하였다. 방법: 1987년부터 2007년 사이에 치료받은 14명의 임신성 유방암환자들의 임상병리적 특성, 치료방법, 생존율을 855명의 40세 미만침윤성 유방암 환자들과 chi-square 검정, Kaplan-Meier 방법, 그리고 Cox`s hazards 모델을 이용하여 비교하였다. 임신성 유방암은 임신기간중또는출산후1년이내에진단된유방암으로정의하였다. 결과: 14명의 임신성 유방암 환자 중, 7명은 임신 중에 진단되었으며, 7명은 산후 1년 내에 진단되었다. 평균 증상기간은 7.6개월이었고, 임신성 유방암과 40세 미만 유방암 환자들의 평균 나이는 각각 32.6세, 34.6세였다 (p=0.044). 임신성 유방암은 모두 유관암이었으며, 병기, 호르몬 수용체 발현, 치료방법은 임신성 유방암과 40세 미만 유방암 사이에 통계적 차이를 보이지 않았다. 임신성 유방암 환자의 5년 무병 생존율과 전체 생존률은 57.1%, 70.0% 였으며, 생존율은 두 그룹 사이에 통계적 차이는 없었다. 다변량 분석에서도 임신여부는 생존율에 영향을 주지 않았다. 임신성 유방암 환자 중 출산전과 후에 따른 생존율은 통계적으로 유의한 차이를 보이지 않았다. 결론: 젊은 유방암 환자와 비교하여 임신성 유방암은 임상병리학적 특성과 예후는 차이가 없었으며, 임신성 유방암 환자와 태아를 위해 적극적인 진단과 다학제적인 치료가 필요하다. Purpose: The aims of this study were to investigate clinicopathological characteristics and outcomes of pregnancy-associated breast cancer (PABC) and to determine the implications of pregnancy itself on the prognosis of PABC. Methods: Clinicopathological features, treatment patterns, and survival of 14 PABC patients were compared to those of 855 invasive ductal carcinoma (IDC) patients under 40 years of age, who were treated between 1987 and 2007, using a chi-square test, the Kaplan-Meier method, and Cox`s hazards models. PABC was defined as breast cancer diagnosed during pregnancy or within the first year after delivery. Results: Among 14 PABCs, 7 were diagnosed during pregnancy and 7, during the first postpartum year. The mean duration of the symptoms was 7.6 months. The mean age at diagnosis of PABC and IDC under 40 years was 32.6 and 34.6 years, respectively (p=0.044). All PABCs were ductal type. Hormone receptors, treatment modalities, and tumor and node stage were not statistically different between PABC and IDC under 40 years. Five-year disease-free, locoregional relapse-free, distant relapse-free, and overall survival of PABC was 57.1%, 71.3%, 56.4%, and 70.0%, respectively. Survival was not significantly different between two groups. In Cox°Øs models, PABC was not associated with survival outcomes. Among PABCs, there was no statistical difference in survival between patients diagnosed before and after delivery. Conclusion: Pregnancy itself does not increase the risk of poorer outcomes among young breast cancer patients. Vigilant diagnosis and multidisciplinary treatment should be recommended to best manage woman with PABC and her baby.