국내 11-12세 소아에서 Td 백신 추가접종의 면역원성과 안전성 평가

이수영,곽가영,목혜린,김종현,허재균,이경일,박준수,마상혁,김황민,강진한,Lee, Soo Young,Kwak, Ga Young,Mok, Hye Rin,Kim, Jong Hyun,Hur, Jae Kyun,Lee, Kyung Il,Park, Joon Su,Ma, Sang Hyuk,Kim, Hwang Min,Kang, Jin Han 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.11

Purpose : This study was undertaken to evaluate the immunogenicity and reactogenicity of Td booster immunization in early preadolescents of Korea. Methods : Healthy preadolescents, who had been vaccinated with 4 or 5 doses of DTaP vaccines until 6 years old age, were enrolled in this study from August 2006 to April 2007. Diphtheria and tetanus anti-toxoid antibodies in sera were measured by ELISA just before vaccination and 4 weeks after vaccination to evaluate immunogenicity. Local and systemic adverse reactions observed for 4 weeks after vaccination to access reactogenicity. Results : 183 preadolescents were enrolled and mean age was $11.40{\pm}0.51$ years old. All subjects achieved seroprotective diphtheria and tetanus anti-toxoid antibodies (titers ${\geq}0.1IU/mL$) after Td booster vaccination. Among 183 vaccinees, 73.8% showed local adverse reactions and 37.2% systemic adverse reactions. Pain at injection site (66.1%) was the most common local reaction, and the most commonly shown systemic reaction was myalgia (17.5%). The adverse reactions were spontaneously relieved within three days after vaccination. Conclusion : Td vaccine in this study was high immunogenic and showed an acceptable tolerance in Korean preadolescents. Td booster vaccination at 11-12 years old is the most effective method to increase compliance of the vaccination and to decrease the incidence of diphtheria and tetanus. 목 적: 11-12세 연령에 Td 백신 1차 추가접종을 하는 방법에 대한 면역원성과 안전성을 평가하기 위해 연구를 계획하였다. 방 법: 2006년 8월부터 2007년 4월까지 연구병원 소아청소년과 외래에 Td 백신 접종을 받기 위해 내원한 11-12세의 소아를 대상으로 하였다. 면역원성을 평가하기 위하여 접종 전 및 접종 4주 후에 혈액을 채취하여 디프테리아 및 파상풍에 대한 항독소 항체가를 측정하였고 이상반응을 평가하기 위해 관찰 일지에 국소 및 전신 이상반응을 기록하였다. 결 과: 총 183명이 연구에 참여하였고 이들의 평균 연령은 $11.40{\pm}0.51$세이었다. Td 백신 접종 전후의 GMC는 디프테리아에 대해서는 10배, 파상풍에 대해서는 26배 이상 증가하였고, 접종 후 디프테리아와 파상풍에 대한 항체 양전율(항체가 ${\geq}0.1IU/mL$ 기준)은 100%이었다. 디프테리아의 접종 전 항체가가 0.1 IU/mL 이상인 피험자는 142명(77.6%)이었고 접종 후 항체가가 1.0 IU/mL 이상인 피험자는 174명(95.1%)이었다. 파상풍의 접종 전 항체가가 0.1 IU/mL 이상인 피험자는 146명(79.8%)이었고 접종 후 항체가가 1.0 IU/mL 이상인 피험자는 181명(98.9%)이었다. 접종 후 국소 이상반응이 73.8%, 전신 이상반응은 37.2%에서 발생하였으나 대부분 3일 이내 소실되었다. 결 론: 매우 높은 면역원성과 심하지 않은 이상반응을 고려할 때, Td 백신의 접종을 11-12세 시행하는 것은 디프테리아와 파상풍에 대한 가장 경제적인 방어 수단이며, 접종 순응도를 효율적으로 높일 수 있는 방법이다.

국가전략소재로서의 희유금속

이수영,지율권,최한신,김용환,이진규,김택수,Lee, Soo-Young,Ji, Yul-Kwon,Choi, Han-Shin,Kim, Hwan-Yong,Lee, Jin-Kyu,Kim, Taek-Soo 한국분말야금학회 2009 한국분말재료학회지 (KPMI) Vol.16 No.3

간(肝) 지방(脂肪) 침윤(浸潤)에 대한 초음파(超音波) 소견(所見)과 혈액(血液) 생화학적(生化學的) 검사(檢査)의 상관관계(相關關係)에 관한 연구(硏究)

이수영,문수형,이화숙,김경희,김강석,Lee, Soo-Young,Moon, Soo-Hyung,Lee, Hoa-Suk,Kim, Kyeong-Hee,Kim, Kang-Seuck 한국생명보험의학회 1985 保險醫學會誌 Vol.2 No.1

In medical selection of Daehan Education Insurance, We doubted that 140 cases(male 117 cases, female 23 cases) might have the fatty infiltration of the liver by ultrasonography. We examined the weight and the hypertension of 140 patients by biochemically analyzing the blood extracted under fasting according to the age-distribution. The results are as follows: 1. 90% out of them had body weight than normal subject. 34.29% out of them were hypertension patients and most of them were $30{\sim}39's$ and $40{\sim}49's$. 2. As compared with the normal subjects in serum lipid value, HDL cholesterol value was low, but total cholesterol, Triglyceride, phospholipid and total lipid value were significantly high. Especially the statistical value of Triglyceride was very significantly high. 3. Total abnormal rate of sGOT value in Liver function test is 34.43% and the mean value of it is 66 unit and the abnormal rate of sGPT value is all 3.14% and the value of it is 70 unit. 4. The abnormal rate of Alkaline phosphatase value is 9.29% and the abnormal rate of total Bilirubine value is 14.29%. As HBsAg positive rate is 2.14% and HBs Ab positive rate is 31.43%, the positive rate of HBsAg represents Lower positive rate than Korean mean value. 5. The abnormal rate of the fasting blood glucose is 15.00% and the abnormal rate of Blood sugar pc 2hrs is 30.71%. 6. The abnormal case of protein, Albumin, Globulin, BUN, craetinine and Hemoglobin value was not found to any of them and in the above liver function test except serum lipid value, the rate not showing the abnormal value represents 45.3%(male) and 52.17%(female).

혈장 세로토닌과 외상후 스트레스 장애 : 월남전 참전 재향군인을 대상으로

이수영,강석훈,정문용,이명희,김태용,소형석,정혜경,최진희,Lee, Soo-Young,Kang, Suk-Hoon,Chung, Moon-Yong,Lee, Myung-Hee,Kim, Tae-Young,So, Hyung-Seok,Chung, Hae-Kyung,Choi, Jin-Hee 대한불안의학회 2009 대한불안의학회지 Vol.5 No.1

Objective : The objective of this study was to examine the relationship between plasma serotonin concentration and posttraumatic stress disorder (PTSD) symptoms in chronic PTSD patients who have been taking medication. Methods : Plasma serotonin level of 14 PTSD patients and a control group of 28 Vietnam War veterans was measured by HPLC (high performance liquid chromatography). The Combat Exposure Scale (CES), Mississippi Scale for Combat-Related Posttraumatic Stress Disorder (M-PTSD), Clinician Administered PTSD Scale (CAPS), Hamilton Rating Scale for Depression (HRSD), and Hamiltion Anxiety Scale (HAS) were used to evaluate PTSD symptom severity. Results : Serotonin level was significantly higher in the PTSD group than in the control group (p=0.036, p=0.006, respectively). M-PTSD (p<0.001), CAPS (p<0.001), HRSD (p<0.001), and HAS (p<0.001) scale scores were significantly higher in the PTSD group than in the control group; however, the CES score failed to show a significant improvement (p=0.964). There were no significant differences between plasma serotonin and PTSD symptoms. Conclusion : In chronic PTSD patients who have been taking medications, we can not predict treatment effect and symptom severity by measuring only plasma serotonin levels. PTSD is a complicated disorder which may likely be related to a variety of neurotransmitter systems. Therefore, further research which investigate relationships with norepinephrine, dopamine, and other neurotransmitters as well as serotonin is needed to improve the treatment of PTSD.

Haemophilus influenzae Type b 에 대한 급성 후두염의 치료 경험 2 례

이수영(Soo Young Lee),유상호(Sang Ho Yoo),이기선(Ki Sun Lee),홍미애(Mi Ae Hong),김순남(Soon Nam Kim) 대한소아알레르기호흡기학회 1998 소아알레르기 및 호흡기학회지 Vol.8 No.1

Epiglottitis is an uncommon but potentially life threatening infectious disease in young children. And it is rapidly progressing cellulitis of the epiglottis and adjacent structures that has the potential for causing abrupt, complete airway obstruction. The most common cause of acute epiglottitis is Haemophilius influenzae type b (Hib), therefore in USA, the acute epiglottitis is seen less commonly since the wide spread use of immunization against Hib. In Korea, there has been no report of acute epiglottitis caused by known bacterial organism, and a little investigation of the effects of the immunization against Hib, or Hib related respiratory diseases. In this report, we describe two cases of acute epiglottitis caused by Hib, occurred in non-immunized young-male children admitted to Ajou University Hospital. They had visited to the emergency center of Ajou University hospital with the complaints of acute fever, drooling, dysphagia and severe respiratory difficulties. In both cases, we observed the cherry-red colored, severely swollen epiglottis by the direct laryngoscopic examination. Hib was cultured in blood samples from both two cases, and the patients treated successfully by the 3 days of endotracheal intubation and proper antibiotics therapy without any complications such as pneumonia, meningitis, osteomyelitis, or pericarditis.

Expression of an artificial gene encoding a repeated tripeptide lysyl-g1utamyl-tryptophan in Tobacco Plant

이수영,나경수,백형석,박희성,조훈식,이용세,최장원,Lee, Soo-Young,Ra, Kyung-Soo,Baik, Hyung-Suk,Park, Hee-Sung,Cho, Hoon-Sik,Lee, Young-Se,Choi, Jang-Won Korean Society of Life Science 2002 생명과학회지 Vol.12 No.1

식물 단백질의 영양가 향상을 위한 일환으로 필수아미노산의 조성이 풍부한 인공단백질을 암호화하는 인공유전자를 담배 식물체에서 발현을 시도하기 위하여, 식물에서 외래유전자의 발현에 널리 사용되는 Cauliflower mosaic virus (CaMV)의 35S promoter를 이중으로 중첩되도록 하고, (Lys-Glu-Trp)이 64번 반복되는 인공유전자 및 nopaline synthase (nos) terminator를 갖고있는 binary vector pART4-4를 구성하였다. 이 재조합 플라스미드는 Agrobacterium tumefaciens를 이용한 형질전환에 의해 Nicotiann tabacum (Var. Xanthi)으로 도입되었다. Kanamycin이 포함된 신초 유도 배지 및 뿌리 유도배지를 이용하여 정상적으로 재생된 담배 식물체로부터 도입된 인공유전자의 발현을 분석하였다. 추출한 genomic DNA를 EcoRI으로 자른 다음 Southern blot 분석에 의하면, 효소 절단 시 예상되는 1.1 kb에서 band를 형성하였으며 각각의 형질전환 식물체에 인공유전자가 1 또는 3 개씩 도입되어 있음을 확인하였다. Northern blot 분석에 의하면 약 1.2 kb 전사체가 비교적 안정하게 발현되었으며, 잎, 줄기, 뿌리로부터 RNA를 분리하여 promoter의 조직 특이성 발현을 분석한 결과, 잎에서 생성되는 RNA가 줄기나 뿌리 조직보다 안정하게 발현되었다. 형질전환 식물체에서 Western blot에 의한 단백질 분석 결과, 잎에서 추출한 단백질로부터 원하는 크기인 33 kDa의 인공단백질이 생성됨을 확인하였으며 발현 수준은 전체 세포 단백질의 0.1%로서 낮은 수준이었다. To investigate expression of the artificial gene encoding a repeated tripeptide lysyl-glutamyl-tryptophan in tobacco plant, the plant binary vector, pART404 has been constructed, which contains the duplicated CaMV 35S promoter, an artificial gene coding for repetitive polymer (Lys-Glu-Trp)$_{64}$, and nopaline synthase (nos) terminator. The recombinant expression vector was introduced in Nicotiana tabacum (var. Xanthi) via Agrobacterium tumefaciens-mediated trans-formation. The transgenic calli selected by kanamycin containing medium were then regenerated to whole plants. Southern blot analysis indicated that five transgenic plants (No. 1, 7, 9, 43, 45) showed the hybridizing signals at 1.1 kb of the expected size on EcoRI digestion and each of the transgenic plants contained 1 or 3 copies of the artificial gene inserted into its genome. By northern blot analysis, the size of the hybridized total RNA was estimated to be approximately 1.2 kb and the RNA appeared generally to have the integrity. Western blot indicated that the protein was detected at the position of 33 kDa and the expression level of the polypeptide in the transgenic plant (No. 45) was measured to approximately 0.1% of the total protein.

이질적 환경을 가지는 백신연구에서 백신효과 추정 방법의 비교연구

이수영,이재원,Lee, Soo-Young,Lee, Jae-Won 한국통계학회 2010 Communications for statistical applications and me Vol.17 No.2

백신연구에서 백신효과를 추정하기 위해 생존시간이 반복 관측되었지만 해석의 용이함과 모형의 편리성 때문에 일반적으로 첫 사건만을 고려한 비례위험모형을 사용해왔다. 그러나 이러한 방법은 실험체들의 감수성과 질병에 대한 노출정도가 이질적인 경우 정보의 손실을 초래할 뿐만 아니라 편향된 결과를 도출한다. 또한 반복 측정된 자료가 서로 독립적이기 보다 상호 연관되어 있을 가능성을 배제할 수 없다. 그러므로 본 연구에서는 시뮬레이션 연구를 통해 다양한 요소가 혼합된 복합적 상황에서 여러 통계적 모형을 이용하여 백신효과를 추정하는 방법들을 비교하였다. In vaccine research, proportional hazards model including only first event have been widely used for estimating vaccine efficacy because it is easy to interpret and convenient. However, this method causes not only loss of information but also biased result when heterogeneity of study subject in exposure and susceptibility exists. Furthermore, it is hard to ignore the possibility that each event is correlated with each other in the repeated events. Therefore, we compare various statistical models to estimate vaccine efficacy under various situations with heterogeneity and event dependency.

Hebb의 학습 법칙과 화소당 가중치 최소화 기법에 의한 적응학습 및 그의 전기광학적 구현

이수영,심창섭,고상호,장주석,신상영,Lee, Soo-Young,Shim, Chang-Sup,Koh, Sang-Ho,Jang, Ju-Seog,Shin, Sang-Yung 대한전자공학회 1989 전자공학회논문지 Vol. No.

Hopfield 모델에 화소당 가주치를 도입하고 이를 최적화하여, 서로간에 상관관계가 높은 "0"에서 "9"까지의 10가지 숫자를 성공적으로 기억, 재생시킬 수 있는 $6{}8$ nodes 연상기억 시스템을 소개한다. 다른 많은 신경회로와는 달리, 이 모델은 "6","8","3","9"와 같이 상관관계가 매우 큰 영상에 대해서도 높은 오차 교정 능력을 가짐을 볼 수 있다. 화소당 가중치의 최적화 무제는 최소자승평균 오차 알고리듬에 기초한 적응학습 과정으로 볼 수 있으며, 이는 또한 Widrow-Hoff 신경회로로 구현 할 수 있다. 가중치 최적화 회로의 전기 . 광학적 구현을 위한 설계도 소개한다. Introducing and optimizing bit-significance to the Hopfield model, ten highly correlated binary images, i.e., numbers "0" to "9", are successfully stored and retrieved in a $6{}8$ node system. Unlike many other neural network models, this model has stronger error correction capability for correlated images such as "6","8","3", and "9". The bit significance optimization is regarded as an adaptive learning process based on least-mean-square error algorithm, and may be implemented with Widrow-Hoff neural nets optimizer. A design for electro-optic implementation including the adaptive optimization networks is also introduced.

생명보험(生命保險) 계약자(契約者)에서 B형간염(型肝炎) 표면항원(表面抗原)과 표면항체(表面抗體) 양성율(陽性率) 조사(調査) 연구(硏究) : vaccine접종(接種) 이후(以後)의 변화(變化) 양상(樣相)

이수영,이화숙,김경희,김강석,Lee, Soo-Young,Lee, Hoa-Suk,Kim, Kyeong-Hee,Kim, Kang-Seuck 한국생명보험의학회 1986 保險醫學會誌 Vol.3 No.1

To investigate the Positive rate of anti HBs and HBs Ag positive rate of the each group of vaccinated hepatitis B type and not-vaccinated, male 4150 female 2475, from May 1, 1984 to November 30, 1985 in the Medical department of Dae han Kyoyuk ins. Co., We examined into the HBsAg and Anti HBs by using the RPHA method and PHA method and the results which were adjusted statistically were as follows. 1. HBs Ag positive rate was 9.0%(228/2531)in 1984 and 7.0%(287/4069) in 1985, the positive rate in 1985 represented 2% lower than in 1984. 2. Anti HBs positive rate was 43.3%(1096/2531) in 1984 and 42.8%(1744/4069) in 1985, there was no significant change for two years. 3. Anti HBs positive rate in the group of the not-vaccinated hepatitis B type was 39.2%(869/2215) in 1984 and 38.8%(1333/3432)in 1985. 4. Anti HBs positive rate in the group of the vaccinated hepatitis B type more than once was 64.7%(419/647)in male and 71.5%(219/306) in female that was 66.9%(638/953) in total. 5. Anti HBs positive rate in the group of the vaccinated hepatitis B type three times was 68.7%(270/393) in male and 80.0%(156/195) in female and 72.4% in total. In this conclusion; The formation of Anti HBs in female showed more or less higher than male. 6. The cases which were detected HBs Ag and anti HBs at the same time were 4 in male and 3 in female and in the group of the vaccinated hepatitis B type, the number of HBs Ag positive cases were 8 in male and 10 in female. (On condition that we didn't distinguish $5{\mu}g/ml$, hepatitis B type vaccine, from $20{\mu}g/ml$ and after vaccinating, the lapsed time was not settled.)

백일해에 대한 연령별 면역혈청학적 연구

이수영,최의윤,김주상,안중현,최정현,마상혁,박준수,김황민,강진한,Lee, Soo Young,Choi, Ui Yoon,Kim, Ju Sang,Ahn, Joong Hyun,Choi, Jung Hyun,Ma, Sang Hyuk,Park, Joon Soo,Kim, Hwang Min,Kang, Jin Han 대한소아감염학회 2012 Pediatric Infection and Vaccine Vol.19 No.2

목적: 우리나라의 백일해에 대한 연령별 방어면역 상태를 평가하고자 면역혈청학적 연구를 시행하였다. 방법: 국내 4개 대학병원에 방문한 건강한 소아, 청소년 및 성인을 대상으로 하였다. 대상을 연령에 따라 7개 연령군으로 구분하였고 ELISA 방법을 이용하여 백일해의 항체가를 측정하였다. 연령군별 기하 평균항체가와 각 연령군별 양성 항체가를 보이는 피험자의 비율을 조사하였다. 제조사의 지침대로, 항체가가 24.0 EU/mL 이상인 경우를 양성으로 판정하였다. 결과: 전체 1,605명의 피험자가 연구에 참여하였고, 기하 평균항체가는 $56.16{\pm}50.54EU/mL$였다. 연령군별로는 11세 미만 소아 연령군($64.78{\pm}53.24EU/mL$)이 가장 높은 기하 평균항체가를 보였다(P<0.001). 양성 항체가를 보인 피험자의 비율은 전체 대상의 68.2%였고, 11세 미만 소아 연령군(76.5%)이 가장 높은 비율을 보였다(P<0.001). 결론: 소아 연령군과 비교하여, 청소년과 성인 연령군에서 백일해에 대한 낮은 기하 평균항체가를 나타냈으며 양성 항체가를 갖는 피험자의 비율이 낮다는 것을 확인하였다. Purpose: We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations. Methods: Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers ${\geq}24.0EU/mL$ were considered to seroprotective as the manufacturer's protocol. Results: Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was $56.16{\pm}50.54EU/mL$. Among seven age groups, age group <11 year showed the highest GMC ($64.78{\pm}53.24EU/mL$) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001). Conclusions: We found that adolescences or adults (age group ${\geq}11$ year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).