인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

헤어리베치 8종의 품종별 국내 적응성 검증을 위해 월동률과 biomass, 그리고 토양 중 무기태질소를 조사하였다. 겨울철 월동 후 생존율은 Hungvillosa와 Ostsaat 품종에서 좋았고, Minnie, Oregon common과 TTF1에서 낮았다. 생존율은 biomass에도 영향을 주어서 Hungvillosa와 Ostsaat 품종에서 가장 높았다. 헤어리베치 품종 간 무기성분 농도는 별다른 차이가 관찰되지 않았다. 토양 중 무기태질소 농도는 질소환원능이 좋았던, Hungvillosa와 Ostsaat가 예초 후 10여일 후에 크게 증가시켰다. This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.2

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다. This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

학교시설 통합정보시스템 구축방안에 관한 연구 - 정보화 구축설계 방향을 중심으로 -

이상민,노근철,유승호,Lee, Sang Min,Ro, Keun Chul,Yoo, Seung Ho 한국교육녹색환경연구원 2017 교육·녹색환경연구 Vol.16 No.1

학교 시설이 가장 중요한 공공시설 중 하나라는 사실에도 불구하고 학교시설에 대한 정보가 체계적으로 관리되지 않고 있는 실정이다. 이에 본 연구에서는 학교시설 정책의 가장 기본적인 요소인 학교시설 현황에 대한 객관적인 데이터를 체계적으로 확보하기 위한 시스템 구축방향에 대한 연구를 진행하였다. 본 연구에서는 학교 시설 정보 관리 절차 및 학교시설의 정보 자료 관리 수준을 향상시킬 수있는 시스템 구축방향을 제안하였으며, 실무적인 활용이 가능한 서비스 제공을 위한 시스템 구축 설계방향을 제시하였다. Despite the fact that school facilities are one of the most important public facilities, information about school facilities is not systematically managed. The purpose of this study was to investigate the direction of system construction to provide objective data on school facilities, which is the basic element of school facility policy. In this study, we propose a system construction method to improve the information management procedure of school facility information and school facilities, and suggest a system design direction for providing service that can be practically used.

Pathogen Inducible Voltage-Dependent Anion Channel (AtVDAC) Isoforms Are Localized to Mitochondria Membrane in Arabidopsis

이상민,My Hanh Thi Hoang,한혜주,김호수,Kyunghee Lee,김경은,Doh Hoon Kim,이상열,정우식 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.3

Voltage-dependent anion channels (VDACs) are reported to be porin-type, β-barrel diffusion pores. They are prominently localized in the outer mitochondrial membrane and are involved in metabolite exchange between the organelle and the cytosol. In this study, we have investigated a family of VDAC isoforms in Arabidopsis thaliana= (AtVDAC). We have shown that the heterologous expression of At- VDAC proteins can functionally complement a yeast mutant lacking the endogenous mitochondrial VDAC gene. AtVDACs tagged with GFP were localized to mitochondria in both yeast and plant cells. We also looked at the response of AtVDACs to biotic and abiotic stresses and found that four AtVDACs transcripts were rapidly upregulated in response to a bacterial pathogen.

소화기질환(消化器疾患)에 사용(使用)된 약주요법(藥酒療法)에 대한 문헌적(文獻的) 고찰(考察)

이상민,강재춘,Lee Sang-Min,Kang Jae-Chun 대한예방한의학회 1999 대한예방한의학회지 Vol.3 No.1

To investigate the documental records of the medi-alcohol theraphy on the Gastrointestinal disease, the 33 kinds of medical books editioned from the old to the present were used. the results were followed Many herbs used on the Gastrointestinal disease were sequently Atractylodis Macrocephalae Rhizoma, Zingiberis Rhizoma, Ginseng Radix. The herbs of invigorating and warming body, regulating the flow of qi etc. were many occupied(used) in treating the Gastrointestinal disease. In analyzing treatment symptom as medi-alcohol theraphy were sequently many used abdominal pain, inappetence, gastrointestinal cancer, indigestion, fatigue, vomiting, diarrhea, abdominal dropsy. The kinds of using medi-alcohol on the Gastrointestinal disease were totally 120 ones.

역사기록물(Archives)의 항구적인 보존화 이용 : 보존전략과 디지털정보화

이상민,Lee, Sang-min 한국기록학회 2000 기록학연구 Vol.0 No.1

In this paper, I examined what have been researched and determined about preservation strategy and selection of preservation media in the western archival community. Archivists have primarily been concerned with 'preservation' and 'use' of archival materials worth of being preserved permanently. In the new information era, preservation and use of archival materials were faced with new challenge. Life expectancy of paper records was shortened due to acidification and brittleness of the modem papers. Also emergence of information technology affects the traditional way of preservation and use of archival materials. User expectations are becoming so high technology-oriented and so complicated as to make archivists act like information managers using computer technology rather than traditional archival handicraft. Preservation strategy plays an important role in archival management as well as information management. For a cost-effective management of archives and archival institutions, preservation strategy is a must. The preservation strategy encompasses all aspects of archival preservation process and practices, from selection of archives, appraisal, inventorying, arrangement, description, conservation, microfilming or digitization, archival buildings, and access service. Those archival functions should be considered in their relations to each other to ensure proper preservation of archival materials. In the integrated preservation strategy, 'preservation' and 'use' should be combined and fulfilled without sacrificing the other. Preservation strategy planning is essential to determine the policies of archives to preserve their holdings safe and provide people with a maximum access in most effective ways. Preservation microfilming is to ensure permanent preservation of information held in important archival materials. To do this, a detailed standardization has been developed to guarantee the permanence of microfilm as well as its product quality. Silver gelatin film can last up to 500 years in the optimum storage environment and the most viable option for permanent preservation media. ISO and ANIS developed such standards for the quality of microfilms and microfilming technology. Preservation microfilming guidelines was also developed to ensure effective archival management and picture quality of microfilms. It is essential to assess the need of preservation microfilming. Limit in resources always put a restraint on preservation management. Appraisal (and selection) of what to be preserved was the most important part of preservation microfilming. In addition, microfilms with standard quality can be scanned to produce quality digital images for instant use through internet. As information technology develops, archivists began to utilize information technology to make preservation easier and more economical, and to promote use of archival materials through computer communication network. Digitization was introduced to provide easy and universal access to unique archives, and its large capacity of preserving archival data seems very promising. However, digitization, i.e., transferring images of records to electronic codes, still, needs to be standardized. Digitized data are electronic records, and st present electronic records are very unstable and not to be preserved permanently. Digital media including optical disks materials have not been proved as reliable media for permanent preservation. Due to their chemical coating and physical character using light, they are not stable and can be preserved at best 100 years in the optimum storage environment. Most CD-R can last only 20 years. Furthermore, obsolescence of hardware and software makes hard to reproduce digital images made from earlier versions. Even if when reformatting is possible, the cost of refreshing or upgrading of digital images is very expensive and the very process has to be done at least every five to ten years. No standard for this obsolescence of hardware and softwa

저항 용접을 이용한 금속 샌드위치 판재 접합에 관한 연구 : Part 2 - 공정해석

이상민,김진범,나석주,Lee Sang-Min,Kim Jin-Beom,Na Suck-Joo 대한용접접합학회 2005 대한용접·접합학회지 Vol.23 No.6

In part 1. optimal process parameters such as thickness of stopper and welding time are achieved to produce high strength ISB(Inner Structured and Bonded) panels. Developed process is different from the usual resistance welding process in the number of points welded at a time. In part 2, Numerical modeling for this new process is proposed and the variation of contact area with respect to the gap of electrodes is studied through FE analyses, Besides, it is tried to figure out the welding nugget formation and proper distance between welding points. FE analytic results show that inner structures are melted more than skin plate, and current distribution between points to be welded can be controlled by distance welding points. Comparison of some FE analytic results with corresponding experimental results could confirm the validity of the proposed numerical modeling.

맥파전달시간과 신체특징 파라미터를 이용한 수축기 혈압측정

이상민,박은경,김인영,김선일,Lee Sangmin,Park Eun Kyung,Kim In Young,Kim Sun I. 대한전자공학회 2005 電子工學會論文誌-SC (System and control) Vol.42 No.3

본 논문에서 맥파전달시간과 신체 특징 파라미터들을 이용하여 비침습적이고 연속적으로 혈압을 측정할 수 있는 방법을 제시하고 임상실험을 통하여 검증하였다. 맥파전달시간은 심전도와 광전용적맥파로부터 구한다. 맥파전달시간만 이용하여 개개인의 혈압을 측정하는 방법이 연구되었지만 이 방법은 모든 사람에게 적용 가능한 범용 회귀식을 도출하기 부족한 면이 있다. 이 연구에서 혈압과 관련되는 신체 특징 파라미터들과 맥파전달시간을 이용하여 모든 사람에게 적용 가능한 범용 회귀식을 도출하여 여러 사람들의 혈압을 측정하였다. 맥파전달시간만 이용하여 만든 회귀방법과 맥파전달시간과 신체 특징 파라미터들을 같이 이용하여 만든 회귀방법을 서로 비교 하였다. 실험 결과, 맥파전달시간과 신체 특징 파라미터를 이용하는 회귀방법이 여러 사람들의 혈압을 더 정확히 측정할 수 있다는 것을 알 수 있었고, American National Standards Institute of the Association of the Advancement of Medical Instrument(ANSI/AAMI)에 더 근접함을 알 수 있었다. In this paper, we proposed noninvasive and continous measurement of blood pressure using pulse transit time(PTT) and physical characteristic parameters and verified its clinical effectiveness. PTT can be obtained by electrocardiogram and phtoplethysmogram. There are many researches for estimating blood pressure using PTT which can be used for individual. However, it is not enough for extracting general regression equation which can estimate blood pressure for unspecified people. In this study, we suggested the regression equatin using PTT and physical characteristic parameters related to blood pressure and did measure blood pressure of many people. we compared the performance between two methods. As the results, we knew that the regressin model using PTT combined with physical characteristic parameters can estimate blood pressure more acurately and is closer to American National Standards Institute of the Association of the Advancement of Medical Instrument(ANSI/AAMI).

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.