Comparison of metabolic profiling of Daphnia magna between HR-MAS NMR and solution NMR techniques

김성혜,이수진,이원호,이유진,최주영,이하니,Youzhen Li,하슬빈,김석만 한국자기공명학회 2021 Journal of the Korean Magnetic Resonance Society Vol.25 No.2

Daphnia magna is used as target organism for environmental metabolomics. The metabolome of D. magna was studied with NMR spectroscopy. Most studies used the extract of D. magna, but the reproducibility cannot be obtained using extracted sample. In this study, lyophilized D. magna samples were analyzed with two different 1H NMR techniques, HR-MAS on intact tissues and solution NMR on extracted tissues. Samples were measured three times using 600 MHz NMR spectrometer. Metabolite extraction required more than twice as many D. magna, but the metabolite intensity was lower in solution NMR. In the spectra of HR-MAS NMR, the lipid signal was observed, but they did not interfere with metabolite profiling. We also confirmed the effect of swelling time on signal intensities of metabolites in HR-MAS NMR, and the results suggest that appropriate swelling should be used in lyophilized D. magna to improve the accuracy of metabolite profiles.

朱의 製法에 關한 硏究

김성혜,이순호,이양호,정춘화 이화여자대학교약학회 1976 梨花藥學硏究 Vol.- No.16

Synthetically, mercuric sulfide has been obtainable by passing H_2S gas through a solution of mercuric chloride or under the presence of alkali polysulfide, by reacting alkali with black mercuric sulfide which is made by mixing mercury with excess sulfur, or it is prepared by passing H_2S gas through the solution of hot mercuric salts containing glacial acetic acid, and excess ammonium thiocyanate. The synthesis of HgS was markedly influenced by some factors, such as, specially, reaction time, reaction temperature, existence of alkali persulfide and alkali concentration.

학교 예술강사 지원제도 개선을 위한 교사 요구 분석

김성혜,강주희 한국미술교육학회 2018 美術敎育論叢 Vol.32 No.2

The purpose of this study is to grasp the current situation of the artist teacher support system and to search for improvement directions. For this purpose, we examined the present status of the artist teacher support system for school and the connection with national curriculum and investigated the needs of school teachers. Based on this, implications for the improvement of the artist teacher support system were derived. The results of this study are as follows: First, need for prior coordination and collaboration between artist teachers and teachers, second, the need for long - term support of artist teachers, third, the necessity of efforts to improve the professionalism of artist teachers, forth, development of support system for enhancing teachers' competence and expertise in arts education, fifth, the need for customized support considering the characteristics and circumstances of each school, sixth, alternatives or solutions to the problems of existing school arts education. 본 연구는 학교 예술강사 지원제도의 실태를 파악하고, 이의 개선 방향을 모색하기 위한 목적으로 실시되었다. 이를 위해 학교 예술강사 지원사업 체계 및 교육과정 연계 지원 현황을 살펴보고 학교 교사들의 요구를 조사·분석하여 학교 예술강사 지원 사업의 실태 및 문제점을 파악하였다. 이를 바탕으로 예술강사 지원사업 개선에 대한 시사점을 도출하였다. 그 결과, 첫째, 예술강사와 교사 간의 프로그램에 대한 사전 조율과 협업의 필요성, 둘째, 장기적인 안목에서의 예술강사 지원의 필요성, 셋째, 예술강사 전문성 신장 및 인식 개선을 위한 노력의 필요성, 넷째, 교사의 예술교육 역량과 전문성 강화를 위한 지원방안 및 지원체제 마련, 다섯째, 각 학교의 특색과 상황을 고려한 맞춤형 지원의 필요성, 여섯째, 기존 학교 예술교육의 문제점에 대한 대안이나 해법 제시 등이 예술강사 지원제도 개선을 위한 시사점으로 도출되었다.

조선 초기 당악용 악기의 종류 고찰

김성혜 민족음악학회 2011 음악과 민족 Vol.41 No.-

As it's discovered that Akhakgwebeom classified the instruments according to their origin, not to kinds of music where they were used, I got interested in what kinds of instruments were used in different kinds of music. What this research focused on is kind of instruments for Dangak. The research was conducted based on the examples of Dangak performances in Joseonwangjosilrok and Akhakgwebeom. The conclusion is as follows;The ceremonies where Dangak was performed were Onemyoin Munsojun, Honjunin Hwidukjun and Sogyeongjun and Eojun Yeayeon. The number of instruments for these ceremonies were no more than 15. They are bak, tungso, dangjuk, dangpiri, banghyang, gyobango, jango, dangbipa, ajang, daejang, guan(or yongguan), sang, hwa, gum, and sul. There was a little difference in number of kinds according to the size of ceremony. 10 of them such as bak, tungso, dangjuk, dangpiri, banghyang, gyobango, jango, dangbipa, ajang, daejang were classified as Dang instruments in Akhakgwebeom, while five of them such as guan(or yongguan), sang, hwa, gum, and sul were classified as A instruments. This proves that these five, formerly recognized as the instruments only for A music, were also used for Dang music. In comparison of the kinds of instruments for court ceremonies, there were differences in kind and number according to the size of ceremony. The number and kind decrease in order of Munsojun-Eojun and Yeayeon-Honjun. It is assumed that the variation must have had a lot to do with the court hierarchy.

경주의 유난곡과 동도국악원의 활동

김성혜 한국국악학회 2011 한국음악연구 Vol.49 No.-

이 글은 오늘날 경주의 국악공연이 존재하는데 공헌한 유난곡(兪蘭谷)이란 인물과 동도국악원(東都國樂院)이란 단체의 의미를 조명한 글이다. 유난곡(1860~1940)은 구한말과 일제강점기에 활동한 경주의 명기(名妓)였는데, 일평생 모은 전 재산을 육영사업과 국악진흥을 위해 1940년에 경주읍에 기증한 사람이다. 유난곡의 헌납 자금은 해방 이후1955년 동도국악원의 설립 및 활동의 원동력이 되었다. 동도국악원의 설립 의미는 일제강점기 때 활약한 풍류인들의 문화공간이 되었고, 전통음악의 명맥을 유지하는 기관이 되었다. 국악원 단원들의 활약은 1960년대 신라문화제 등 시민 축제 때 국악공연을 주도하였고, 무엇보다 중요한 것은 경주향제줄풍류의 명맥을 유지하고 전승한데 음악사적 의의가 크다. This is to illuminate a person named Yu Nangok and a music organization,Dongdogugagwon, for their contribution to the current traditional music performance in Gyeongju. Yu Nangok, who had been a famous gisaeng in the late Joseon and the Japanese colonial rule period, donated her entire fortune to Gyeongju-eup for education and promotion of traditional music in 1940. Her donation played a major role in the establishment of Dongdogugagwon in 1955 and its operation. During the Japanese colonial rule, Dongdogugagwon provided artists with space for cultural activities and helped them keep on doing traditional music. Its members led the traditional music performance in the city festivals such as Silla Culture Festival in 1960s. Above all, its contribution to Korean music history is found in the maintenance and continuation of Gyeongjuhyangjejulpungryu.

발해 정효공주묘 동·서 벽화의 재해석

김성혜 한국음악사학회 2008 한국음악사학보 Vol.41 No.-

Tomb of Princess Chŏnghyo 貞孝公主 is the only Parhae 渤海 tomb that has a mural in it. In addition, its significance gets greater owing to the musician images in the mural. The present writer saw the copy of the mural at the Parhae Relic Exhibition for the first time. As I examined it, I discovered a few problems in the exiting interpretation of the murals on the east and west walls. The purpose of this research is to attempt to reinterpret it, highlighting the musical instruments and chimul 持物 (lit. holding material), which the persons in the murals hold. The conclusion is as follows; Chimul wrapped in a covering cloth, and held by the second person in the mural of the west wall, has been accepted as pakp'an 拍板 (wooden clapper) so far. However, it would look rectangular rather than conical when it's wrapped in a covering cloth. The most likely instrument that is in the shape of cone is 'saeng' 笙 (mouth organ). It has been introduced as a Koguryŏ 高句麗 instrument in the historical documents. There's a case in which it was used in the Unified Silla era. In the year of 725, it was played along with konghu 箜篌 (harp) appeared in a Bramba bell at Sangwŏnsa Temple. Because it's also found along with konghu in the mural of Princess Chŏnghyo Tomb, it's strongly believed to be saeng. Judging from the perfect symmetry of the east and west walls, in terms of the arrangement of the people, the official hats they don and chimul they hold, it's considered that the east and west walls represent the same thing. From this point of view, chimul that the three persons on the east wall hold must be musical instruments because the things that the three persons on the west wall have are musical instruments. They are likely to be tongbal 銅鈸 (a percussion), so 簫 (panpipes), and yogo 腰鼓 (waist drum), respectively. Since the mural of Princess Chŏnghyo Tomb is the only archeological specimens available, it was indispensable to make a through study on the images in it for Korean music study. The further study on Parhae music is anticipated, based upon the result of this research, to find out about what Parhae music shares in common with that of the neighboring countries, such as T'ang 唐 China and the Unified Silla Kingdom, and how unique it is compared with the music of those countries. 정효공주묘는 발해 고분 가운데 유일하게 벽화가 남아 있는 것이다. 더욱이 악인상(樂人像)이 그려진 것으로는 유일한 고분이라 할 수 있다. 필자가 이 고분의 벽화 모사도를 발해유적전시관에서 처음 접하면서, 동·서벽을 판독한 기존 견해에 문제점을 발견하여 이를 재해석하고자 본고를 마련하였다. 특히 동서 벽면의 구성과 벽화 속에 그려진 인물의 악기와 지물에 대해 음악학적 관점에서 재해석하고자 했는데, 결론을 요약하면 다음과 같다. 정효공주묘 서벽 둘째 인물의 보에 싼 지물은 지금까지 박판으로 판독되었으나, 박판의 경우 보에 싸면 벽화 모습처럼 원추형이 아니라 장방형을 이루기 때문에 타당하지 않다고 보았다. 외형이 원추형을 이루는 악기로 가장 유력한 것은 ‘생’이며, 이 악기는 고구려의 악기로 문헌기록에 등장한 바 있다. 그리고 통일신라의 경우 725년 상원사 범종에서 공후와 함께 연주된 사례가 있는데, 발해의 정효공주묘 벽화 역시 공후가 생과 함께 있기도 하여 필자는 생으로 보았다. 다음 동벽의 구성이 인물의 배치와 관모 그리고 보에 싼 지물 등이 정확히 서벽과 대칭을 이루기 때문에 동·서의 성격을 같은 것으로 이해하였다. 즉 서벽의 세 인물이 든 지물이 모두 악기였듯이, 동벽의 세 인물이 든 지물 역시 악기로 보았으며, 그것은 각각 타악기인 동발과 관악기인 소로 판독하였고 나머지 1점은 요고일 가능성을 제시하였다. 발해음악사에서 고고학 자료로 유일한 것이 정효공주묘의 벽화인 만큼 이 벽화에 나타난 도상은 음악학적 관점에서 1차적으로 세밀한 관찰이 이루어져야 하겠기에 본고를 마련하였다. 이제 이 연구를 바탕으로 비록 고분 1기에 지나지 않지만, 발해음악은 주변국인 통일신라음악 및 당나라 음악과 어떠한 보편성과 특수성이 있는지 규명해야 하는 과제가 남아 있다.

한국 찬송가의 역사적 고찰과 그 문제점

김성혜 호서대학교 사회과학연구소 1981 社會科學硏究 Vol.1 No.1

고종 친정 직후 정치적 기반 형성과 그 특징(1874~1876)

김성혜 한국근현대사학회 2010 한국 근현대사 연구 Vol.52 No.-

After restoring sovereignty, Kojong(고종) began to reform Daewongun(대원군)’s power basis and policies, maintaining Daewongun’s power collective structure with the exception of few pro-Daewongun forces and placing relatives from his maternal side and Jonchinseonpa(종친선파) members in important governmental positions of power. In addition, Kojong retained those policies that contributed to the strengthening of the royal family’s and the central government’s authority, abolished taxes established under Daewongun’s rule, and prohibited the circulation of Chinese currency to shut out Daewongun’s financial resources. However, these policies and actions by Kojong triggered resistance from Daewongun and numerous appeals for Daewongun’s return to the capital city out of his retirement in Yangju(양주), destabilizing Kojong’s political foundation during the early stages of his regime. In response, Kojong implemented strict punishment against those who submitted such appeals and even outlawed the submission itself, in an effort to stabilize his country. He also permitted the resignation of Yeonguijeong(영의정) Lee Yu-won(이유원) under questions of responsibilities over political affairs, and inaugurated Jwauijeong(좌의정) Lee Choi-eung(이최응) and Uuijeong(우의정) Kim Byeong-guk(김병국) systems. This was a move to eliminate Daewongun’s justification for his return to the political arena through the appointment of Lee Choi-eung, a royal, and simultaneously win over Noron(노론), which centered on Ahndong Kims. And Kojong’s heightened awareness of Daewongun and pro-Daewongun forces as threats ultimately led to Kojong’s intensified ruling of the nation based on a power structure surrounding close associates.

급성 골수성 백혈병환자에서 발생한 중심정맥관 관련Mycobacterium mucogenicum 균혈증 1예

김성혜,정우진,최상호,김미나,김양수,우준희 대한내과학회 2006 대한내과학회지 Vol.71 No.5

Mycobacterium mucogenicum is the one of rapidly growing mycobacteria identified in 1995; so far, only few cases have been reported that were associated with dialysis equipment or central venous catheter. Here we present a central catheter-related M. mucogenicum bloodstream infection in a 48-year-old woman with acute myeloid leukemia. She presented with fever and was empirically treated with ceftazidime and piperacillin. Since the fever resolved within 24 hours, she underwent bone marrow transplantation as scheduled. Four days before stem cell infusion, M. mucogenicum grew from the blood cultures that were drawn through a central venous catheter and from peripheral blood. The central venous catheter was removed and the cultured organism was identified to be M. mucogenicum by sequence analysis of the 16S ribosomal RNA. She was further treated for 8 weeks with cefoxitin and amikacin as the bacteria were confirmed to be susceptible by broth microdilution test. There has been no episode of recurrence during the 6-month follow-up.(Korean J Med 71:582-586, 2006) Mycobacterium mucogenicum은 색소를 형성하지 않는 신속발육 항산균의 일종으로 드물게 상처 관련 감염이나 중심 정맥관 균혈증을 일으킬 수 있다. 중심 정맥관을 가지고 있었던 면역기능 저하 환자의 혈액배양 여러 쌍에서 그람 양성 간균이 자랄 때 가능성을 염두에 두어야 하며 정확한 진단과 치료를 위해서는 16S RNA 염기서열 분석 등의 분자생물학적 기법을 이용하여 신속히 동정하는 것이 바람직하다. 저자들은 급성 골수성 백혈병으로 히크만 중심 정맥관을 가지고 있던 환자에서 발열이 있어 시행한 혈액배양 검사상 자란 신속 발육 항산균을 16S RNA를 이용하여 M. mucogenicum으로 신속히 동정하였고, cefoxitin과 amikacin을 투여하여 치유하였기에 문헌고찰과 함께 보고하는 바이다.

송방송의 고대음악사 중 자료 분야 연구 업적

김성혜 민족음악학회 2022 음악과 현실 Vol.- No.64

This paper examines Song Bangsong’s achievements on Korean music history study centering around the records on Korean ancient music history. It is identified that he published 10 papers in which he either translated or annotated and indexed ancient music records. His accomplishments in the 10 papers are as follows. First, compared to those of the first generation music scholars, the 10 papers could be viewed as prolific. Secondly, he revised, annotated and indexed the original of “Samguksagi Jabji Akjo”. It is a significant accomplishment in that it laid the academic foundation for Korean ancient music history. Thirdly, he indexed the 4 kinds of ancient music records. His index enabled other researchers in the field to make use of the historical materials more conveniently. This contributes a lot to the study of ancient music history. Fourthly, he annotated the historical records of East Asian music that forms the framework of ancient music history. He studied and annotated the contents on Korean ancient music from the five kinds of domestic, Chinese, and Japanese historical materials. Finally, he translated the foreign studies on Korean ancient music. Music of the Ancient Silk Road by Kishibe Shigeo(岸邊成雄) and Study on Yeonakjo of Sui(隋) and Tang(唐) Dynasties by Hayashi Kenzo(林謙三) were translated by Guo Moruo(郭沫若), a Chinese scholar, in Chinese. Song Bangsong translated them again in Korean. Through these translations, he contributed to improving the understanding of the musical trend in the surrounding countries of Korea.