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쥐의 간 미토콘드리아 Aldehyde Dehydrogenase 의 분포와 특성에 관한 연구
최주영,주충노 ( Joo Young Choi,Chung No Joo ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
Separation of inner membrane, outer membrane and matrix were accomplished by treating rat liver mitochondria preparation with digitonin followed by differential centrifugation. Mitochondrial ALDH isozymes were founded to be located in outer membrane as well as matrix fraction. Soluble low K_m ALDH in matrix had a very low K_m ($lt;10^(-6)M) for acetaldehyde, but the K_m of soluble high K_m ALDH in the matrix was 9.8 × 10^(-4) M, outer membrane bound ALDH showed a relatively high K_m, 1.4 × 10^(-2)M. The outer membrane bound ALDH was purified and characterized. Optimal pH was 9.5 and pI was 6.1. Subunit molecular weight was determined by SDS-PAGE and found to be 55,000. These results suggest that the soluble low K_m ALDH might be primarily response for the oxidation of acetaldehyde in rat liver, but the outer membrane bound ALDH will serve as barrier when the concentration of acetaldehyde in cytosol is high.
쥐의 간 시토졸 aldehyde Dehydrogenase 의 면역학적 특성
최주영,주충노 ( Joo Yong Choi,Chung No Joo ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.6
Specificity for cytosolic ALDH was investigated through ouchterlony double immunodiffusion and western blotting. The precipitin band formed by antigen-antibody was positive for protein staining as well as enzyme activity staining, indicating that an interaction of the antibody with antigenic determinants of the enzyme did not destroy a catalytic activity of this enzyme. It was realized that the immunological activity of rat liver cytosolic ALDH is quite specific and differs from those of other ALDH isozymes such as bovine liver cytosol ALDH and outer-membrane bound and matrix ALDH isozymes of rat liver. Results of activity staining of rat cytosol ALDH showed that antigenic determinant site is not active site.
쥐의 간 시토졸 Aldehyde dehydrogenase 의 정제 및 특성에 관한 연구
최주영,주충노 ( Joo Young Choi,Chung No Joo ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.1
Cytosolic ALDH of rat liver was purified by ammonium sulfate precipitation, DEAE-Sephacel, CM-cellulose ion exchange chromatography, Sephacryl S-300 gel filtration, and 5`-AMP Sepharose 4B affinity chromatography. The molecular weight was determined by Sephacryl S-300 gel filtration and found to be 217,000 dalton. SDS-PAGE showed that it was homotetramer consisting of four identical subunits whose molecular weight was 53,000 dalton. Optimal pH of this enzyme was 9.5 and pI value was 6.75. K_m value of rat liver cytosolic ALDH for acetaldehyde was 1.06×10^(-3) M, but those of indole-3-acetaldeghyde($lt;10^(-6) M) and benzaldehyde (1.59×10^(-5) M) were extremely low. These results suggested that rat liver cytosolic ALDH might be primarily involved in the oxidation of aromatic aldehyde including biogenic aldehyde.
최주영,박성준,Choi, Joo-Young,Park, Sung-Jun 한국교육시설학회 2019 敎育施設 Vol.26 No.3
This study aims to suggest a Biophilic classroom design of high school to obtain attention restoration for students. The learning space for youth in the modern society is composed of dry artificial structures. This space is considered to be a space that can not relieve stress caused by learning. "The Attention Restoration Theory" is divided into "Directed Attention" of humans, which is the cause of fatigue and stress, and "Involuntary Attention" as a solution to it. "Involuntary Attention" takes place in a rest state and helps the brain recover when exposed to nature. And the core of "Biophilic-Design Theory" is that humans can recover physical and mental conditions when exposed to nature. The purpose of this study is to apply "The Biophilic-Design Theory" that emphasizes the importance of exposure to nature to the educational space and plan the space where the 'Attention Restoration' can be achieved. The research method is as follows. First, we review previous studies related to "The Biophilic-Design Theory" and "The Attention Restoration Theory". Second, we analyze the application examples of "The Biophilic-Design Theory" and "The Attention Restoration Theory" in domestic and foreign educational spaces. Third, the concept of educational space is set up based on the elements derived from previous studies. Finally, we propose the planning direction of classroom design based on Biophilic-Design. The following conclusions were drawn. First, The creation of the education space to restore the learner's attention requires a visual space plan that utilizes natural elements such as natural light, artificial light, plants, and natural materials that can directly experience nature. Second, the direction in which students in the classroom can be "The Attention Restoration Theory" should consider the use of indirect natural elements that bring the surrounding natural landscape into the interior. This study will be used as the baseline data for the spatial design and planning of education facilities based on Biophilic-Design.
최주영 한국도시부동산학회(구 도시정책학회) 2016 도시부동산연구 Vol.7 No.1
A criteria of golf course development is regarded as significant alternative associated with landscape of forest as well as reckless development. There are two purpose of the paper. First, this study introduces the major factors for a golf course site criteria. Secondly, it estimates the significance of the factors and relative imporance between factors. A comparision of their relative importance was made using the Analytic Hierarchy Process(AHP). As a resurts of this study, Charcterist of ecolgical environment was the most important factorIn in firstly hierarchy. Charcterist of slope was the most important factor in secondly hierarchy. Charcterist of water source protection areas was the most important factor in thirdly hierarchy.