The rationale and potential for using Lactobacillus in the management of periodontitis

Wang Jiaqi,Liu Yingman,Wang Weiru,Ma Jiaojiao,Zhang Manman,Lu Xiaoying,Liu Jie,Kou Yurong 한국미생물학회 2022 The journal of microbiology Vol.60 No.4

Periodontitis refers to a wide range of the inflammatory conditions of supporting dental structures. For some patients with periodontitis, antibacterial agents are needed as an adjuvant to mechanical debridement treatments and oral hygiene maintenance. However, the widespread use of broad-spectrum antibiotics for the prophylaxis and treatment of periodontal infections results in the emergence of resistant pathogens. Therefore, probiotics have become markedly interesting to researchers as a potentially safe alternative to periodontal treatment and maintenance. Probiotics have been used in medicine for decades and extensively applied to the treatment of inflammatory diseases through the modulation of microbial synergy and other mechanisms. A growing amount of evidence has shown that using Lactobacillus strains for oral cavity maintenance could improve periodontal health. In this study, we reviewed studies showing proof of the inhibitory effects of Lactobacillus species on periodontal inflammation. We also explored the rationale and potential for using Lactobacillus species in the management of periodontitis.

Host-guest doping induced excited state energy transfer for efficient room temperature phosphorescence emission

Yurong Guo,Yanan Wang,Yingdan Gao,Jingran Zhang,Chao Wang,Guangjiu Zhao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-

The 1,8-naphthalimide derivatives as the guest and the benzophenone (BP) analogues bis(4-chlorophenyl)methanone (BP2C) and bis(4-fluorophenyl)methanone (BP2F) benzophenone derivativesas the host, we constructed a series of novel doping systems with fluorescence and room temperaturephosphorescence (RTP) dual emission. The steady-state spectroscopy, ultrafast spectroscopy techniques,and theoretical calculations indicate that Förster resonance energy transfer (FRET) between singlet statesbetween host and guest components enables energy redistribution in doped systems. At the same time,the Dexter energy transfer (DET) between the triplet states realizes the obvious absorption of the excitedtriplet state of the guest, resulting in the long-lived room temperature phosphorescence of the doped system. Doping materials have unlimited potential for information storage and anti-counterfeiting byexploiting the properties of photo activation.

CDs/ZnO composite material with solid state fluorescence performance for quantitative determination of methyl red content and antibacterial properties

Mengqi Wang,Kaiyue Zhang,Feixiang Ji,Yurong Guo,Chao Wang,Shiping Wang,Ya Chu,Guangjiu Zhao 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-

A water-soluble probe composite material (CDs/ZnO) was synthesized by microwave pyrolysis methodsof citric acid, urea and zinc oxide (ZnO) with aggregation-induced emission activity. We creatively realizedthe composite of CDs and nano-ZnO in the form of foam, then used as bactericide for the first timeand showed excellent antibacterial properties. Also, the composite materials overcame the problem ofaggregation-induced quenching (ACQ) and realized solid-state emission. The materials had two differentemission centers, and the two emission peaks were varied with the change of excitation wavelength. Furthermore, Methyl red (MR) showed fluorescence quenching for CDs/ZnO with obvious linear correlation,which demonstrated potential in the measurement of MR in dyeing wastewater. The CDs/ZnO weobtained perfectly inherited the advantages of CDs and nano ZnO, it has not just no cytotoxicity to cells,but also has a good killing effect on bacteria. Hence, this work shows significant adaptable fluorescencebasedprotocol with enormous potential applications in biology.

Fumigant Activity of Volatiles from Streptomyces alboflavus TD-1 against Fusarium moniliforme Sheldon

Zhifang Wang,Changlu Wang,Fengjuan Li,Zhenjing Li,Mianhua Chen,Yurong Wang,Xi Qiao,Hong Zhang 한국미생물학회 2013 The journal of microbiology Vol.51 No.4

The fumigant activity of volatiles generated by Streptomyces alboflavus TD-1 against Fusarium moniliforme Sheldon was investigated. The results showed that the mycelial growth,sporulation, and spore germination of F. moniliforme were significantly suppressed, and that membrane permeability was disrupted in the presence of the volatiles. Gas chromatography-mass Spectrometry analysis revealed 31 kinds of volatile organic compound from the volatiles. Among them,two earthy-smelling substances, namely, 2-methylisoborneol (50.97%) and trans-1,10-dimethyl-trans-9-decalinol (3.10%)were found. The most abundant compound, 2-methylisoborneol,exhibited inhibitory activity against F. moniliforme by fumigation. All these results suggested that S. alboflavus TD-1 can be a promising starter for the inhibition of F. moniliforme through fumigant action.

Feeding behavior of the notorious invasive Mealybug, Phenacoccus solenopsis to exotic weeds using EPG

Wang Qianjin,Qi Guojun,He Yurong,Lyu Lihua 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is notorious for causing severe economic losses to a wide range of crop species. It is noteworthy that this mealybug performed different host preference and fitness within their host range. In order to determine the preference of P. solenopsis between economic plants and weeds, the feeding behavior of cotton mealybug on three widespread exotic weeds belong to Asteraceae family (Bidens pilosa L., Wedelia trilobata and Ambrosia artemisiifolia.) were determined and compared with cotton Gossypium hirsutum L. using electrical penetration graph (EPG). Compared with G. hirsutum, cotton mealybug took the shortest time for trying/detecting on leaf surface and spend the longest duration times for ingestion from phloem bundle tissues feeding on A. artemisiifolia. Results showed that A. artemisiifolia was the most adaptable host for cotton mealybug, B. pilosa and W. trilobata were less suitable ones, implying these plants vulnerable to damage by this mealybug. This consequence gave more evidence for the feeding harm of P. solenopsis and might provide a scientific basis for explain if exotic weeds play an important role in the regional dispersal, and exist as transfer host for pest divert to farmland during uncultivated season.

Walking patterns of Trichogramma chilonis and Trichogrammatoidea bactrae upon vegetable leaf surfaces

Desen WANG,Xiaofang HE,Chengyin ZHAO,Yurong He,Lihua LU,Yongli LUO,Hualong QIU 한국곤충학회 2020 Entomological Research Vol.50 No.1

Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) and Trichogrammatoidea bactrae Nagaraja (Hymenoptera: Trichogrammatidae) are egg parasitoids of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). We observed and recorded the walking patterns of T. chilonis and T. bactrae females on leaves of Raphanus sativus L., Brassica juncea (L.) Czern. et Coss., Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee, and Brassica oleracea L. Our analysis indicated that Trichogramma females spent more time and moved more slowly on R. sativus leaf surface, compared with the other vegetable leaf surfaces. In addition, Trichogramma females were more likely to walk in straight line on B. oleracea leaf surfaces than R. sativus, B. juncea, and B. campestris leaf surface. Compared to T. bactrae females, T. chilonis females spent significantly less time on the leaf surface, and the walking path of T. chilonis was less affected by leaf surface characters (e.g., trichomes, wrinkle, and waxes). During the period of residence on the leaf surface, parasitoid females spent more than 87.8% of their time moving. This study demonstrates that vegetable leaf surface can influence Trichogramma’s walking pattern while they are foraging for hosts.

Effects of Gal-13 on the Content of Immunoglobulin, Proliferation of Lymphocyte and Antibody Titers after Vaccination with Infectious Bursal Disease Virus Vaccine in Chickens

Yang, Yurong,Jiang, Yibao,She, Ruiping,Peng, Kaisong,Zhou, Xuemei,Yin, Qingqiang,Wang, Decheng,Liu, Tianlong,Bao, Huihui Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.3

Gal-13 is an antimicrobial peptide isolated from chicken intestine. Ninety chickens were randomly divided into two groups (45 chickens for each group) to determine the effect of oral administration of Gal-13 on the acquired immune response. The chickens in the first group were fed a diet without Gal-13 as the control, and the chickens in the second group were fed the same diet, except that Gal-13 ($1{\mu}g/ml$) was suspended in drinking water just after hatching. Samples of blood, thymus, bursa of fabricius and spleen were taken at day 1, 4, 7, 10 and 17. The chickens in both groups received infectious bursal disease virus vaccine at day 20, and then sera samples were collected for analysis at 14, 21, 28 and 35 days after vaccination. The results showed: (1) Gal-13 could enhance the content of immunoglobulin (Ig)G at the age of 4 to10 days (p<0.05) and IgM at the age of 4 and 10 days (p<0.05) in the serum; (2) In vitro experiments showed that Gal-13 (0.625-1.250${\mu}g/ml$) enhanced the proliferation of peripheral blood lymphocytes of the chickens stimulated by lipopolysaccharide (LPS) and concanavlin A (ConA). Compared to the control, Gal-13 (1 ${\mu}g/ml$) enhanced the proliferation of bursa lymphocytes at 17 days of age (p<0.01) and thymus lymphocytes at 7 days of age (p<0.01), but restrained lymphocyte proliferation in chicken spleen and differed significantly at day 10 (p<0.01); (3) Gal-13 enhanced infectious bursal disease virus antibody in sera of chickens 21 days after infectious bursal disease virus vaccine administration (p<0.05). These results suggested that Gal-13 could modulate adaptive immune responses of chickens.

Effect of Laser Power on Anisotropic Microstructure and Mechanical Behavior of Biomedical Ti-35Nb-15Zr (at%) Alloy Fabricated by Laser Powder Bed Fusion

Jun Zhou,Yurong Wang,Geng Zhi,Lugui He 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.1

In this study, the anisotropy of Ti-35Nb-15Zr (at%) fabricated in situ using laser powder bed fusion (LPBF) was investigated for the first time. The effects of varying laser powers on the anisotropic microstructure and mechanical properties were investigated. The equiaxed grains growing in the horizontal section were finer than the β-columnar grains growing in the vertical section, and the grain size increased from 8.8 to 10.47 μm as the laser power increased. The difference in microstructure generally resulted in higher nanohardness in the horizontal Sect. (7.12, 6.70, and 6.49 GPa at 250, 275, and 300 W laser power, respectively) than in the vertical Sect. (5.41, 5.56, and 5.38 GPa at 250, 275, and 300 W laser power, respectively). The yield strength (1223.48 ± 27.67 MPa) and ductility (6.77 ± 0.48%) of the samples were both higher in the horizontal direction. The Young’s modulus of the samples in the horizontal direction, which reached as low as 72.82 ± 0.77 GPa, were lower than those in the vertical section, with a minimum of 77.59 ± 1.89 GPa. With increasing laser power, the yield strength in the horizontal direction, Young’s modulus, and yield strength in the vertical direction increased, whereas the yield strength in the vertical direction decreased. In particular, the Ti-35Nb-15Zr alloy exhibited better cellular activity than cast Ti6Al4V. All samples formed dense, bone-like apatite layers on the surface in different directions, demonstrating the great potential of the LPBF Ti-35Nb-15Zr alloy as an implant.

Five Inputs Code Lock Circuit Design Based on DNA Strand Displacement Mechanism

Jixiang Li,Yurong Li,Junwei Sun,Yanfeng Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.11

In recent years, the development of biological computers is becoming faster and faster, in order to make the logical operation algorithms of biological computers more mature and stable, a new idea for the code lock logic circuit is proposed based on DNA strand displacement by using the dual-rail method. The code lock is designed by four input signals and one conversion input signal. Only when the four input codes are correct and the conversion signal code is turned on, the password lock will be in open state, otherwise the password lock will produce an alarm signal, stopping outside invasion timely. The information of key is processed to obtain the correct password; finally, the experimental simulation results are obtained by Visual DSD software. The results analysis show that the designed code lock circuit is effective, which may provide a good technical support and a good theoretical basis in biological computers development.

Effects of soil-treatment with fungal biopesticides on pupation behaviors, emergence success and fitness of tea geometrid, Ectropis grisescens (Lepidoptera: Geometridae)

TaoMa,Huifang Wang,Shiping Liang,Qiang Xiao,Panrong Cao,Xuan Chen,Ying Niu,Yurong He,Zhaohui Sun,XiujunWen,Cai Wang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1

Ectropisgrisescens Warren (Lepidoptera: Geometridae) is one of the most severe defoliating pests of tea plants. Synthetic pesticides have been widely applied to control this pest in tea plantations, but pesticide residues may decrease the quality and safety of tea products. In the present study, we hypothesized that soil treatment with the two commercial biopesticides (Shuiguxin ® ) based on Metarhizium anisopliae (Metchnikoff) Sorokin and Beauveria bassiana (Balsamo) could reduce the survivorship and fitness of E. grisescens. Wandering larvae of E. grisescens were allowed to pupate in soil treated with each biopesticide, and the concentrations of M. anisopliae (Shuiguxin ® ) and B. bassiana (Shuiguxin ® ) that produced the 50% mortality values (LC 50 ) were 2.9 × 10 6 and 1.6 × 10 7 conidia/g soil, respectively. Artificial burying the pupae using soil treated with M. anisopliae (Shuiguxin ® ) and B. bassiana (Shuiguxin ® ) (1 × 10 8 or 1 × 10 9 conidia/g soil for both biopesticides) also significantly reduced emergence success of E. grisescens. In addition, choice tests showed that soil treated with the high concentration of M. anisopliae (Shuiguxin ® ) or B. bassiana (Shuiguxin ® ) had repellent effects on pupating E. grisescens. However, sublethal concentrations (LC 25 and LC 50 ) of both biopesticides did not significantly affect fecundity, fertility and longevity of post-emerged adults. Our study showed that soil treatment with the two commercial biopesticides caused direct mortality of pupating E. grisescens, but may not effectively suppress E. grisescens populations at sublethal concentrations. The realistic application of the fungal dosage in fields should be determined in future studies