A Hierarchical Segmentation Approach towards Roads and Slopes for Collapse Recognition

Yuanyuan Jia,Zhongshi He,Huazheng Zhu 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.5

Fractionation and Characterization of ε-Poly-L-lysine from Streptomyces albulus CGMCC 1986

Shiru Jia,Yujie Dai,Baoqing Fan,Guoliang Wang,Yuanyuan Jia,Peng Peng 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2

ε-Poly-L-lysine (ε-PLL) produced by Streptomyces albulus CGMCC 1986 was fractionated using ultra-filtration technique with 2 and 5 kDa cut-offs of membrane. The number-average molecular weight of each fraction was determined by 1H nuclear magnetic resonance (NMR)method. The number-average molecular weights of the cutoffs of 5 and 2 kDa and the filtrate are 4,230.95, 3,687.80,and 1,900.82 Da, respectively. 1H NMR indicates the chemical shifts of α-H, β-H, γ-H, δ-H, and ε-H are very similar to all the fractions. Fourier transform-infrared (FTIR)spectra showed that the ε-PLL solid samples obtained by freeze-drying at pH 5 with molecular weights higher than 2 kDa take on a β-turn conformation, however, the fraction with molecular weight smaller than 2 kDa adopts random coil structure. The antibacterial test proved that the fraction between 2 and 5 kDa of membranes behaves the highest antibacterial activity than other fractions for the test strains of Staphylococcus aureus, Micrococcus luteus,Bacillus subtilis, Escherichia coli, and Shigella.

Effect of light quality on regeneration and naphthoquinones accumulation of Arnebia euchroma

Jia Hu,Yao Leng,Yuanyuan Jiang,Su Ni,Li Zhang 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.4

The influence of light quality on the regeneration (i.e., formation, proliferation, growth, development, and rooting) and naphthoquinones’ accumulation of Arnebia euchroma was investigated in vitro. The cotyledons of sterile seedings were placed on modified LS medium supplemented with 0.8 mg/L thidiazuron to obtain callus, which was incubated on the corresponding medium at different culture stages under varying light conditions, including dark, fluorescent lamp (Fl), white light-emitting diodes (LEDs), blue LEDs, red LEDs, and a combination of 25% red, 25% blue, and 50% white LEDs [RBW (1:1:2)]. During the establishment of the A. euchroma regeneration system, we obtained the maximum fresh weight, dry weight, germination rate, shoot number, and proliferation rate under RBW (1:1:2) LEDs in the formation and multiplication phases; the growth index, shoot height, leaf number, and photosynthetic pigment concentration were promoted under red LEDs in the growth and development phase; and the maximum rooting rate (89.29% ± 2.06%) was observed under red LEDs in the rooting phase. To study the effect of light quality on naphthoquinones’ accumulation of A. euchroma, we used the euphylla to achieve adventitious roots, and the highest shikonin (121.77 ± 6.68 μg/g DW) and β′β-dimethylacrylalkannin (619.18 ± 15.71 μg/g DW) contents were detected in the dark culture. The lowest naphthoquinone contents were recorded under Fl in the adventitious roots. Overall, RBW (1:1:2) LEDs enhanced A. enchroma formation and proliferation, while red LEDs promoted the plant’s growth, development, and rooting. The dark condition was beneficial to naphthoquinones’ accumulation, whereas the light condition inhibited the naphthoquinones production of A. enchroma adventitious roots. Determining the suitable light quality for the regeneration and accumulation of naphthoquinones that is conducive to the protection and promotes resource utilization is essential.

Synthesis and Gas Adsorption Properties of Carbide-Derived Carbons from Titanium Tin Carbide

Yuanyuan Zhu,Aiguo Zhou,Jin Jia,Junjun Wang,Jiang Liu,Baolin Xing,Chuanxiang Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.4

Here we reported the synthesis of nanoporous carbide-derived carbons (CDCs) from a new precursor, titanium tin carbides (Ti2SnC), via chlorination at 400–1100℃. At low chlorination temperature (400–500℃), as-synthesized CDCs mainly consisted of amorphous carbon and chlorides. As the chlorination temperature increased up to 600℃, chlorides disappeared, and the main composition of CDCs was amorphous carbon. At high chlorination temperature, there was a trend of graphitization. The microstructure of CDCs was observed and characterized by scanning electron microscopy and transmission electron microscopy. Some graphite-like sheet structures in CDCs were found. Specific surface area (SSA) and pore volume of CDCs increased with chlorination temperature, except an abnormal decrease of the CDC chlorinated at 900℃. CDC chlorinated at 1100℃ had the largest SSA, 1580 m2 /g. In order to apply these materials as novel hydrogen/methane storage media in the area of energy efficient transport, gas adsorption properties of CDCs were measured. For CDC chlorinated at 1100℃, pore volume uptakes are 206 cm3 /g at 60 bar (25℃) for methane, and ~442 cm3 /g at 35 bar (-196℃) for hydrogen, respectively. It was suggested that CDCs from Ti2SnC are promising materials for hydrogen/ methane adsorptive storage.

Optimization of basic magenta adsorption onto Fe/Cu nanocomposites synthesized by sweet potato leaf extract using response surface methodology

Yuanyuan Niu,Ruijuan Jia,Chenglin Liu,Xiuli Han,Chun Chang,Junying Chen 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.8

Green synthesis of metal nanoparticles using plant extracts as an effective bio-reducing reagent has attracted considerable attention. Fe/Cu nanocomposites synthesized by extracts of sweet potato leaves served to remove basic magenta (BM) from aqueous solution. The adsorption operation conditions of BM on Fe/Cu nanocomposites were optimum by Box-Behnken design (BBD) model of response surface methodology (RSM). The adsorption equilibrium data were well described by the Sips and Redlich-Peterson models. The thermodynamic studies showed that the adsorption process was endothermic and spontaneous. The maximum adsorption capacity from the Sips model was 235.92mg/g at 298 K, which indicated that Fe/Cu nanocomposites had potential application in wastewater treatment. As indicated by pseudo-second order kinetics model, the adsorption of BM onto Fe/Cu nanocomposites could be achieved through the complexation, H-bonding, - adsorbate-adsorbent interaction, and electrostatic interaction at different pH values

Mechanism and Analysis of Laccase-mediated Coloration of Silk Fabrics

Weini Jia,Qiang Wang,Xuerong Fan,Aixue Dong,Yuanyuan Yu,Ping Wang 한국섬유공학회 2018 Fibers and polymers Vol.19 No.4

Laccase provides a mild and eco-friendly alternative for the dyeing of fabrics. In this study, laccase-mediated catalytic oxidation was employed in coloration of silk fabrics, and the color was then assessed. The surfaces of silk fabrics were characterized by scanning electron microscope (SEM), atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and X-ray photoelectron spectroscopy (XPS). Their thermal and crystallization properties were also investigated by differential scanning calorimetry (DSC), thermogravimetry (TG), and Xray diffraction (XRD). The textile softness was evaluated by the bending rigidity (B). In addition, high performance liquid chromatography (HPLC) and ultraviolet-visible (UV-VIS) spectroscopy were employed to analyze hydrolysate of silk fabrics obtained from hydrolysis with hydrochloric acid solution. The results demonstrated that a stable reddish-yellow layer was formed on the surface of silk fabrics by the laccase-mediated coupling of phenol hydroxyl side chains in the silk polypeptide. Moreover, the SEM and AFM observations showed that the surface of colored silk fiber was slick. ATR-FTIR and XPS results demonstrated differences in the C, N, O contents and the functional groups of the uncolored and colored silk fabrics. The DSC, TG, and XRD indicated that the thermal properties of silk fabrics were not affected by laccase and the coupling reaction mainly occurred in the amorphous region. The DMF extraction test further illustrated that covalent bonds were formed between tyrosine residues constituent of silk peptides. Finally, HPLC and UV-VIS results showed that new substances were formed as a result of conjugation between benzene rings.

Whole RNA-sequencing and gene expression analysis of Trichoderma harzianum Tr-92 under chlamydospore-producing condition

Min Yuan,Yuanyuan Huang,Zhenhua Jia,Weina Ge,Lan Zhang,Qian Zhao,Shuishan Song,Yali Huang 한국유전학회 2019 Genes & Genomics Vol.41 No.6

Background Trichoderma is one of the most important biocontrol fungi, which could produce mycelia, conidiospores, and chlamydospores three types of propagules under different conditions. Chlamydospores are produced in harsh conditions in various fungi, and may be more resistant to adverse conditions. However, the knowledge associated with the mechanism of chlamydospore formation remained unclear in Trichoderma. Objectives This study is aimed to explore the essential genes and regulatory pathways associated with chlamydospore formation in Trichoderma. Methods The culture condition, survival rate, and biocontrol effects of chlamydospores and conidiospores from Trichoderma. harzianum Tr-92 were determined. Furthermore, the whole transcriptome profiles of T. harzianum Tr-92 under chlamydospore-producing and chlamydospore-nonproducing conditions were performed. Results T. harzianum Tr-92 produced chlamydospores under particular conditions, and chlamydospore-based formulation of T. harzianum Tr-92 exhibited higher biocontrol ability against Botrytis cinerea in cucumber than conidoiospore-based formulation. In the transcriptome analysis, a total of 2,029 differentially expressed genes (DEGs) were identified in T. harzianum Tr-92 under chlamydospore-producing condition, compared to that under chlamydospore-nonproducing condition. GO classification indicated that the DEGs were significantly enriched in 284 terms among biological process, cellular components and molecular function categories. A total of 19 pathways were observed with DEGs by KEGG analysis. Furthermore, fifteen DEGs were verified by quantitative real-time PCR, and the expression profiles were consistent with the transcriptome data. Conclusion The results would provide a basis on the molecular mechanisms underlying Trichoderma sporulation, which would assist the development and application of fungal biocontrol agents.

Analysis of the Bacterial Community in a Full-scale Printing and Dyeing Wastewater Treatment System Based on T-RFLP and 454 Pyrosequencing

Qingxiang Yang,Jia Wang,Xinkuan Han,Yuanyuan Xu,Dong Liu,Hongxin Hao,Xuemei L,Yuhui Guo,Tianqi Niu,Shiyue Qi 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

In this study, the bacterial dynamics andstructure compositions in the two-stage biological processof a full-scale printing and dyeing wastewater (PDW)treatment system were traced and analyzed by terminalrestriction fragment length polymorphism (T-RFLP) and454 pyrosequencing techniques. T-RFLP analysis showedthat the microbial communities experienced significantvariation in the process of seed sludge adaptation to thePDW environments and were in constant evolution duringthe whole running period of the system, despite theconstant COD and color removal effects. Pyrosequencingresults indicated that the two-stage biological systemharbored rather diverse bacteria, with Proteobacteria beingthe predominant phylum during the steady running period,although its microbial compositions differed. The firststageaerobic tank was dominated by α-Proteobacteria(89.05% of Proteobacteria), whereas in the second-stageaerobic tank, β- and γ-Proteobacteria, besides α-Proteobacteria,were the dominant bacterial populations.

Characterization of Bacteriostatic Sausage Casing: A Composite of Bacterial Cellulose Embedded with ε-Polylysine

Huixia Zhu,Shiru Jia,Hongjiang Yang,Weihua Tang,Zhilei Tan,Yuanyuan Jia 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6

The feasibility of a novel bacteriostatic sausage casing made of bacterial cellulose (BC) embedded with ε-polylysine (ε-PL) was evaluated. The ε-PL/BC composite was prepared by immersing BC tubes in ε-PL solution and its characteristics were analyzed with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD),and atomic force microscopy (AFM) techniques, respectively. The results suggested that ε-PL molecules were incorporated into the cellulose fiber networks and the ε-PL/BC composite might have a novel unique structure. No significant loss of antimicrobial activity was observed even after autoclaving at 121℃ for 30 min and the oxygen permeability was far below than that of polyethylene (PE) and polyvinyl alcohol (PVA) membrane. Its tensile strength was 51.8 MPa. The ε-PL/BC composite exhibits bacteriostatic and/or bacteriocidal activities against a broad spectrum of Gram-positive and Gram-negative bacteria; as a result, an extended shelf life than controls was observed for sausage packaged with the ε-PL/BC composite.

Caption Region Detection in Video Images by Improved Corner Detector

Xin Liu,Jin Dai,Yuanyuan Jia,Rubin Liu 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.4