림프부종 치료프로그램이 림프부종이 있는 말기 암 환자의 사지둘레와 신체증상에 미치는 영향

박명희,허수진,최은미,정유니 병원간호사회 2006 임상간호연구 Vol.11 No.2

Purpose: This study was performed to investigate the effects of lymphedema treatment program on extremities circumference and physical symptoms in terminal cancer patients with lymphedema. Method: The subject was 13 patients diagnosed with lymphedema and admitted to C Hospital, hospice unit from March 1 to August 31, 2004. The effects of treatment were evaluated by measuring extremities circumferernce and by assessing physical symptons. Data were analyzed by using repeated measures ANOVA, Scheffé multiple comparisons. Result: The results were as follows: Extremities circumference was reduced significantly 30.8cm after last treatment compared with 33.4cm pre-treatment. Physical sysptom, 'heaviness' was improved significantly 4.2 after last treatment compared with 7.8 pre-treatment and difficulty to mobility was improved significantly 4.5 after last treatment compared with 7.4 pre-treatment. Conclusion: These findings indicate that lymphedema treatment program could be an effective nursing intervention for reducing extremities circumference and improving physical symptoms in terminal cancer patient.

애니메이션에 활용할 영웅 김유신 캐릭터 복식의 배색계획

이수니(Su Ni Lee),박영실(Young Sil Park) 산업기술교육훈련학회 2017 산업기술연구논문지 (JITR) Vol.22 No.4

This research case lies in suggesting color arrangement planning to make it possible to express the image of a traditional Korean hero. Among others, this research is intending to show color arrangement planning in order for Korean color image to be highlighted more by inquiring into the traditional color expression of a character centering on the hero of the Three States-General “Kim Yu-shin”. The color arrangement case of ‘Kim Yu-shin’ character as a Korean traditional hero aims at suggesting the method of selection & application of traditional color through their concretization on the basis of the understanding the traditional clothing during the period of the Three States. Accordingly, this research is intending the color planning of a character on the basis of the contents of an ancient writing during the period of the Three State 〈The Heritage of the Three States〉 & the existing researches on Korean traditional color. In addition, this research is intending to make it possible to apply and use the traditional color for a character easily and readily by suggesting color arrangement planning through the expression of Korean traditional color applied to a character in digital color. The color planning for a Korean traditional hero character belongs to the case which is helpful to reproducing the traditional image of a character by coming close to the prototype image of Korea to the maximum through the use of Korean traditional color; further, it is also of a great help to the Korean traditional character development industry.

Chemopreventive Effects of Korean Angelica versus Its Major Pyranocoumarins on Two Lineages of Transgenic Adenocarcinoma of Mouse Prostate Carcinogenesis

Tang, Su-Ni,Zhang, Jinhui,Wu, Wei,Jiang, Peixin,Puppala, Manohar,Zhang, Yong,Xing, Chengguo,Kim, Sung-Hoon,Jiang, Cheng,Lü,, Junxuan American Association for Cancer Research 2015 CANCER PREVENTION RESEARCH Vol.8 No.9

<P>We showed previously that daily gavage of <I>Angelica gigas</I> Nakai (AGN) root ethanolic extract starting 8 weeks of age inhibited growth of prostate epithelium and neuroendocrine carcinomas (NE-Ca) in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Because decursin (D) and its isomer decursinol angelate (DA) are major pyranocoumarins in AGN extract, we tested the hypothesis that D/DA represented active/prodrug compounds against TRAMP carcinogenesis. Three groups of male C57BL/6 TRAMP mice were gavage treated daily with excipient vehicle, AGN (5 mg per mouse), or equimolar D/DA (3 mg per mouse) from 8 weeks to 16 or 28 weeks of age. Measurement of plasma and NE-Ca D, DA, and their common metabolite decursinol indicated similar retention from AGN versus D/DA dosing. The growth of TRAMP dorsolateral prostate (DLP) in AGN- and D/DA-treated mice was inhibited by 66% and 61% at 16 weeks and by 67% and 72% at 28 weeks, respectively. Survival of mice bearing NE-Ca to 28 weeks was improved by AGN, but not by D/DA. Nevertheless, AGN- and D/DA-treated mice had lower NE-Ca burden. Immunohistochemical and mRNA analyses of DLP showed that AGN and D/DA exerted similar inhibition of TRAMP epithelial lesion progression and key cell-cycle genes. Profiling of NE-Ca mRNA showed a greater scope of modulating angiogenesis, epithelial–mesenchymal transition, invasion–metastasis, and inflammation genes by AGN than D/DA. The data therefore support D/DA as probable active/prodrug compounds against TRAMP epithelial lesions, and they cooperate with non-pyranocoumarin compounds to fully express AGN efficacy against NE-Ca. <I>Cancer Prev Res; 8(9); 835–44. ©2015 AACR</I>.</P>

Performance of a hydrofoil operating close to a free surface over a range of angles of attack

Ni Zao,Dhanak Manhar,Su Tsung-chow 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

Performance of a NACA 634-021 hydrofoil in motion under and in close proximity of a free surface for a large range of angles of attack is studied. Lift and drag coefficients of the hydrofoil at different submergence depths are investigated both numerically and experimentally, for 0 AoA30 at a Reynolds number of 105. The results of the numerical study are in good agreement with the experimental results. The agreement confirms the new finding that for a submerged hydrofoil operating at high angles of attack close to a free surface, the interaction between the hydrofoil-motion induced waves on the free surface and the hydrofoil results in mitigation of the flow separation characteristics on the suction side of the foil and delay in stall, and improvement in hydrofoil performance. In comparing with a baseline case, results suggest a 55% increase in maximum lift coefficient and 90% average improvement in performance for, based on the lift-to-drag ratio, but it is also observed significant decrease of lift-to-drag ratio at lower angles of attack. Flow details obtained from combined finite volume and volume of fluid numerical methods provide insight into the underlying enhancement mechanism, involving interaction between the hydrofoil and the free surface.

The Korean Society of Gastroenterology & SIDDS 2043 : Slide Session ; S-GIO-03 : GI Oncology ; Role of Follow Up Endoscopic Examinations in Response Assessment of Patients with Gastric Diffuse Large B Cell Lymphoma

( Ha Ni Lee ),( In Seok Lee ),( Chul Hyun Lim ),( Jin Su Kim ),( Yu Kyung Cho ),( Jae Myung Park ),( Bo In Lee ),( Sang Woo Kim ),( Myung Gyu Choi ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: According to guidelines of lymphoma, gastric diffuse large B cell lymphoma (DLBCL) patients are supposed to perform CT and/or PET CT regularly for response assessment after treatment such as chemotherapy and radiation therapy. However, endoscopic examinations and biopsies are not indicated for follow up guidelines. The aim of this study was to investigate the necessity and effi cacy of endoscopic examinations when following up during and after treatment of tumor. Methods: This was a retrospective study of 44 consecutive patients who were diagnosed with gastric diffuse large B cell lymphoma, treated and underwent serial follow up endoscopies and biopsies from July 2009 to April 2014 in Seoul St. Mary`s hospital. Endoscopic examinations and biopsies were performed at the time of diagnosis and after treatment. Disappearance of mucosal lesion except atrophy, discoloration, and neovascularization was defi ned as endoscopic remission. We followed the terms of NCCN guidelines (complete remission, partial response, stable disease, progressive disease) to determine clinical response with CT and/or PET CT. Results: A total of 161 endoscopic examinations were performed (median 3.7; range 2-7). Within a median follow up period of 27 months (range 4-57), 39 (89%) patients achieved complete remission in clinical response, while endoscopic remission was found in 34 (77%) patients. Only 32 (73%) patients achieved both forms of remission. 2 of 5 patients who achieved complete remission in clinical response but had remnant lesion at endoscopic examinations have recurred after several months. Conclusions: In gastric DLBCL patients, endoscopic response not always correlates with clinical response, and it also predicts the recurrence of disease as well. Therefore, we suggest that follow up endoscopic examinations and biopsies should be performed in addition to radiologic examinations such as CT and PET CT.

Development and Evaluation of Simultaneous Quantification of Naringin, Prunin, Naringenin, and Limonin in Citrus Juice

Hui Ni,Su Fang Zhang,Qiu Feng Gao,Yang Hu,Ze Dong Jiang,Feng Chen 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4

Quantification of limonin, naringin, prunin, and naringenin is an important approach for monitoring of debittering processes of citrus products. Naringin and limonin have different polarity and solubility values, causing difficulty in simultaneous extraction and quantification. A procedure combining HPLC and solid-phase extraction was developed to simultaneously quantify these analytes in citrus juice. Analytes exhibited calibration curves of good linearity along with low limit of detection and limit of quantification values. Naringin, prunin, naringenin, and limonin exhibited respective recovery values of 92.2-100.6, 92.0-97.3, 98.1-102.2, and 102.4-103.9%, respectively. Relative standard deviations were lower than 5%. For analysis of naringin, prunin, naringenin, and limonin in citrus juices, the simultaneous method displayed analytical results identical to traditional respective quantification methods. The simultaneous method is highly effective for monitoring naringin, prunin, naringenin, and limonin levels in citrus juice.

Ginsenoside Rg3 ameliorates myocardial glucose metabolism and insulin resistance via activating the AMPK signaling pathway

Jingyu Ni,Zhihao Liu,Miaomiao Jiang,Lan Li,Jie Deng,Xiaodan Wang,Jing Su,Yan Zhu,Feng He,Jingyuan Mao,Xiumei Gao,Guanwei Fan 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.2

Background: Ginsenoside Rg3 is one of the main active ingredients in ginseng. Here, we aimed to confirm its protective effect on the heart function in transverse aortic coarctation (TAC)-induced heart failure mice and explore the potential molecular mechanisms involved. Methods: The effects of ginsenoside Rg3 on heart and mitochondrial function were investigated by treating TAC-induced heart failure in mice. The mechanism of ginsenoside Rg3 for improving heart and mitochondrial function in mice with heart failure was predicted through integrative analysis of the proteome and plasma metabolome. Glucose uptake and myocardial insulin sensitivity were evaluated using micro-positron emission tomography. The effect of ginsenoside Rg3 on myocardial insulin sensitivity was clarified by combining in vivo animal experiments and in vitro cell experiments. Results: Treatment of TAC-induced mouse models with ginsenoside Rg3 significantly improved heart function and protected mitochondrial structure and function. Fusion of metabolomics, proteomics, and targeted metabolomics data showed that Rg3 regulated the glycolysis process, and Rg3 not only regulated glucose uptake but also improve myocardial insulin resistance. The molecular mechanism of ginsenoside Rg3 regulation of glucose metabolism was determined by exploring the interaction pathways of AMPK, insulin resistance, and glucose metabolism. The effect of ginsenoside Rg3 on the promotion of glucose uptake in IR-H9c2 cells by AMPK activation was dependent on the insulin signaling pathway. Conclusions: Ginsenoside Rg3 modulates glucose metabolism and significantly ameliorates insulin resistance through activation of the AMPK pathway.

Identification of signal peptide domain SOST mutations in autosomal dominant craniodiaphyseal dysplasia.

Kim, Su Jin,Bieganski, Tadeusz,Sohn, Young Bae,Kozlowski, Kazimierz,Sem?nov, Mikhail,Okamoto, Nobuhiko,Kim, Chi Hwa,Ko, Ah-Ra,Ahn, Geung Hwan,Choi, Yoon-La,Park, Sung Won,Ki, Chang-Seok,Kim, Ok-Hwa,Ni Springer-Verlag 2011 HUMAN GENETICS Vol.129 No.5

<P>Sclerosteosis and Van Buchem disease are related recessive sclerosing bone dysplasias caused by alterations in the SOST gene. We tested the hypothesis that craniodiaphyseal dysplasia (CDD) (MIM 122860), an extremely rare sclerosing bone dysplasia resulting facial distortion referred to as 'leontiasis ossea', could also be caused by SOST mutations. We discovered mutations c.61G>A (Val21Met) and c.61G>T (Val21Leu) two children with CDD. As these mutations are located in the secretion signal of the SOST gene, we tested their effect on secretion by transfecting the mutant constructs into 293E cells. Intriguingly, these mutations greatly reduced the secretion of SOST. We conclude that CDD, the most severe form of sclerotic bone disease, is part of a spectrum of disease caused by mutations in SOST. Unlike the other SOST-related conditions, sclerosteosis and Van Buchem disease that are inherited as recessive traits seem to be caused by a dominant negative mechanism.</P>

Integration of single-cell transcriptomics and epigenetic analysis reveals enhancer-controlled TIMP1 as a regulator of ferroptosis in colorectal cancer

Li Meng,Ni Qian-yang,Yu Su-yang 한국유전학회 2024 Genes & Genomics Vol.46 No.1

Background Ferroptosis is an iron-dependent non-apoptotic programmed cell death. However, the regulatory mechanism of ferroptosis in colorectal cancer (CRC) is still unclear. Objective The aim of this study was to investigate the role and mechanism of enhancer-controlled genes in ferroptosis in CRC. Methods Dimensionality reduction and differentially expressed genes (DEGs) identification were conducted using Seurat algorithm based on single-cell RNA sequencing (scRNA-seq) data from the GSE200997 dataset. Ferroptosis-related pathway enrichment analysis was performed using the FerrDb V2 database. Enhancers were identified using HOMER algorithm based on H3K27ac ChIP-seq data from the GSE166254 dataset. Kaplan-Meier Plotter online tool was used to analyze prognosis and gene expression correlation. Transcription factors were predicted using the transcription factor affinity prediction web tool. The binding of enhancer to transcription factor and H3K27ac enrichment were detected by ChIP-qPCR. RSL3 was used to induce ferroptosis in CRC cells. Gene transcription was detected by qRT-PCR. Cell proliferation was detected by CCK8 assay. Results Nine cell clusters including T cells, natural killer cells, macrophages, mast cells, epithelial cells, fibroblasts, goblet cells, B cells and dendritic cells were identified in CRC and normal colonic tissue samples. Compared to normal colonic tissue-derived epithelial cells, 1075 DEGs were screened in CRC tissue-derived epithelial cells. Ferroptosis-related pathway enrichment suggested that DEGs were associated with the regulation of ferroptosis. DPEP1, ETV4, CEBPG, TIMP1, DUOX2 and LCN2 were identified as the significantly upregulated genes enriched in the “ferroptosis regulator” term, and their H3K27ac signals were significantly higher in CRC tissues than in normal colonic tissues. Of these, only the expression of TIMP1 predicted a poor prognosis of CRC patients. Transcription factor SPI1 drove TIMP1 transcription by binding to its enhancer. Overexpression of TIMP1 significantly promoted the resistance to ferroptosis induced by RSL3 in CRC cells, which was partially restored by SPI1 knockdown. Conclusion Transcription of TIMP1 was driven by transcription factor SPI1 in combination with its enhancer, consequently promoting CRC cells against ferroptosis. The SPI1/TIMP1 axis confers ferroptosis resistance in CRC, and thus has the potential to be the molecular targets for CRC treatment. Background Ferroptosis is an iron-dependent non-apoptotic programmed cell death. However, the regulatory mechanism of ferroptosis in colorectal cancer (CRC) is still unclear. Objective The aim of this study was to investigate the role and mechanism of enhancer-controlled genes in ferroptosis in CRC. Methods Dimensionality reduction and differentially expressed genes (DEGs) identification were conducted using Seurat algorithm based on single-cell RNA sequencing (scRNA-seq) data from the GSE200997 dataset. Ferroptosis-related pathway enrichment analysis was performed using the FerrDb V2 database. Enhancers were identified using HOMER algorithm based on H3K27ac ChIP-seq data from the GSE166254 dataset. Kaplan-Meier Plotter online tool was used to analyze prognosis and gene expression correlation. Transcription factors were predicted using the transcription factor affinity prediction web tool. The binding of enhancer to transcription factor and H3K27ac enrichment were detected by ChIP-qPCR. RSL3 was used to induce ferroptosis in CRC cells. Gene transcription was detected by qRT-PCR. Cell proliferation was detected by CCK8 assay. Results Nine cell clusters including T cells, natural killer cells, macrophages, mast cells, epithelial cells, fibroblasts, goblet cells, B cells and dendritic cells were identified in CRC and normal colonic tissue samples. Compared to normal colonic tissue-derived epithelial cells, 1075 DEGs were screened in CRC tissue-derived epithelial cells. Ferroptosis-related pathway enrichment suggested that DEGs were associated with the regulation of ferroptosis. DPEP1, ETV4, CEBPG, TIMP1, DUOX2 and LCN2 were identified as the significantly upregulated genes enriched in the “ferroptosis regulator” term, and their H3K27ac signals were significantly higher in CRC tissues than in normal colonic tissues. Of these, only the expression of TIMP1 predicted a poor prognosis of CRC patients. Transcription factor SPI1 drove TIMP1 transcription by binding to its enhancer. Overexpression of TIMP1 significantly promoted the resistance to ferroptosis induced by RSL3 in CRC cells, which was partially restored by SPI1 knockdown. Conclusion Transcription of TIMP1 was driven by transcription factor SPI1 in combination with its enhancer, consequently promoting CRC cells against ferroptosis. The SPI1/TIMP1 axis confers ferroptosis resistance in CRC, and thus has the potential to be the molecular targets for CRC treatment.

Chrysophanol-induced Necrotic-like Cell Death through an Impaired Mitochondrial ATP Synthesis in Hep3B Human Liver Cancer Cells

Chien-Hang Ni,Jing-Gung Chung,Po-Yuan Chen,Hsu-Feng Lu,Jai-Sing Yang,Hui-Ying Huang,Shin-Hwar Wu,Siu-Wan Ip,Chin-Tung Wu,Su-Yin Chiang,Jaung-Geng Lin,W. Gibson Wood 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.5

Liver cancer is the most common form of cancer in Taiwan and it usually responds to chemotherapy. However, patients often have side effects to the chemotherapeutic drugs. Thus new agents are urgently required to treat liver cancer. Chrysophanol, one of the anthraquinone derivatives, was reported to inhibit some human cancer cell growth which may be due to the induction of apoptosis similar to other anthraquinone derivatives though such actions have not been reported. In the present study, we reported that chrysophanol inhibits cell growth in Hep3B liver cancer cells based on the following observations: 1) induc cell morphological changes; 2) decreased percentage of viable cells; 3) induced S phase arrest of cell cycle progression; 4) induced DNA damage as measured by comet assay and DAPI staining. Chrysophanolinduced cell death however, seems to be related to necrotic processes rather than typical apoptosis. Chrysophanol induced reactive oxygen species and Ca2+ production and decreased mitochondrial membrane potential (ΔΨm) and ATP levels in Hep3B cells. No effects were observed on known protein regulators of apoptosis such as Bax and Bcl-2. Chrysophanolinduced cell death took place independently of caspase-8 and -9. Based on our findings, we propose that chrysophanol reduces cellular ATP levels causing a drop in energy resulting in necrotic-like cell death.