제주 성상 S/S MTr #1 행원 풍력단지 전압해석

김상준(Sang-Jun Kim),조민호(Min-Ho Cho),윤기갑(Gi-Gab Yoon),장상옥(Sang-Ok Jang),안정식(Jeong-Shik Ahn) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.11

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

( Ji Yong Kim ),( Jai Sung Lee ),( Yong Seok Han ),( Jun Hee Lee ),( Inhyu Bae ),( Yeo Min Yoon ),( Sang Mo Kwon ),( Sang Hun Lee ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although H2O2 (200 mM) increased intracellular ROS levels in human MSCs, lycopene (10 μmM) pretreatment suppressed H2O2-induced ROS generation and increased survival. H2O2-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by H2O2 treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

S-344 : The Role of Skeletal Muscle Mass in Development of Metabolic Syndrome

( Byung Sam Park ),( Jun Sung Moon ),( Ji Sung Yoon ),( Kyu Chang Won ),( Hyoung Woo Lee ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Introduction: It has been well known that abdominal adiposity is related with insulin resistance and greater risk of metabolic disorder. Skeletal muscle plays central role in insulin mediated glucose disposal of whole body, but we only know a little about the association between skeletal muscle mass and MetS (MetS). The aim of this study is to clarify the clinical role of skeletal muscle mass in developing MetS through using skeletal muscle parameters by body impedance analysis (BIA). Methods: 1,042 healthy adults aged from 20 to 75 years who visited Yeungnam university health promotion center from Jun. 2008 to Jun. 2010 were enrolled. 204 subjects who had prior MetS or chronic disease that can affect to skeletal muscle mass were excluded. After 24 months from baseline, the metabolic parameters were assessed and the development of MetS was diagnosed using modified NCEP-ATP III criteria. From the BIA (Inbody 720), we obtained skeletal muscle mass (SMM, Kg), body fat mass (BFM, Kg), and visceral fat area (VFA, cm2). Then, we had defined as follows; percent of skeletal muscle mass (SMM%, %): SMM (kg)/weight (Kg), skeletal muscle index (SMI, Kg/m2): SMM (Kg)/height (m)2, skeletal muscle to body fat ratio (MFR): SMM (kg)/BFM (Kg) and skeletal muscle to visceral fat ratio (SVR, Kg/cm2): SMM (Kg)/VFA (cm2) Results: Mean follow up periods were 28.7±5.4 months. Among total 838 subjects (46.9±9.9 years, M:F=477:361), 88 (10.5%) were newly diagnosed MetS. 5th quintile of the SMM%, MFR and SVR was associated with decreased risk of development of MetS after adjusting for confounding factors. Conclusion: Decreased skeletal muscle mass may play critical role in development of the MetS and not absolute amount of skeletal muscle mass but relative ratio to body composition may be more important.

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

Kim, Ji Yong,Lee, Jai-Sung,Han, Yong-Seok,Lee, Jun Hee,Bae, Inhyu,Yoon, Yeo Min,Kwon, Sang Mo,Lee, Sang Hun The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although $H_2O_2$ ($200{\mu}M$) increased intracellular ROS levels in human MSCs, lycopene ($10{\mu}M$) pretreatment suppressed $H_2O_2$-induced ROS generation and increased survival. $H_2O_2$-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by $H_2O_2$ treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

동종 반월상 연골 이식술의 임상적 결과 - 내측과 외측 및 동반 손상의 유무에 따른 비교 -

조승목,윤경호,이정환,배대경,송상준,강창민,Cho, Seung-Mok,Yoon, Kyoung-Ho,Lee, Jung-Hwan,Bae, Dae-Kyung,Song, Sang-Jun,Kang, Chang-Min 대한관절경학회 2009 대한관절경학회지 Vol.13 No.1

목적: 동종 반월상 연골 이식술의 결과를 내측과 외측 및 동반손상 유무에 따라 비교하고자 하였다. 대상 및 방법: 1999년 12월부터 2007년 6월까지 동종 반월상 연골 이식술을 시행하고 1년이상 추시가 가능했던 52례를 대상으로 하였다. 내측 이식군은 19례, 외측 이식군은 33례였고, 단독 손상군은 18례 동반 손상군은 34례였다. 평균나이는 34.2세(18세~51세) 평균 추시기간은 42.2개월(12개월~90개월)이었다. 임상적 결과의 판정을 위하여 슬관절 운동범위, VAS 점수, IKDC 주관적 평가 점수, Lysholm 점수, Tegner 점수, 환자 만족도 및 이차 관절경 또는 추시 MRI를 시행하였다. 결과: 수술 후 평균 관절운동 범위는 130.3도 였다. VAS 점수는 수술 전 5.96점에서 3.05점으로, IKDC 주관적 평가 점수 46.5점에서 64.52점으로(p<0.001), Lysholm 점수는 61.94점에서 79.58점으로(p=0.0019) Tegner점수는 수술 전 2.97에서 3.62점으로 향상되었으며 수술의 만족도는 '매우만족', '대체로 만족'이라고 대답한경우가 61.5% 였다. 수술 후임상적 점수의 향상이 있었지만 내측과 외측 이식술 군간 그리고 단독 손상군과 동반 손상군 간의 통계적 유의한 차이는 없었다. 이차 관절경을 시행한 18례중 10례에서 이식된 동종 반월상 연골의 변연부의 치유 소견이 관찰되었고 6례에서 부분파열이 2례에서는 복합 파열이 관찰되었다. 추시 MRI 촬영이 가능하였던 16례에서 아탈구는 12례에서 관찰되었다. 결론: 반월상 연골 이식술 후 내측과 외측 이식군 간의 임상적 결과는 차이가 없었으며 동반된 연골 손상이나 불안정성에 대한 치료를 병행한 경우 동반손상의 유무는 결과에 영향을 미치지 않았다. Purpose: To compare the clinical outcomes after meniscus allograft transplantation between lateral and medial or isolated and combined procedure groups. Materials and Methods: Of the patients who had undergone arthroscopic meniscal allograft transplantation between Dec. 1997 and Jun. 2007, 52 patients were available for retrospective evaluation. Patients were grouped into lateral(33 cases) and medial(19 cases) transplant groups as well as those with isolated(18 cases) and combined(34 cases) procedure. The average age was 34.2 years and the mean follow-up period was 42.2 months. Postoperative range of motion (ROM), visual analog scale (VAS), International Knee Documentation Committee (IKDC) subjective score, Lysholm score, Tegner score, patient's subjective satisfaction, $2^{nd}$ look arthroscopy and MRI were evaluated retrospectively. Results: Mean postoperative ROM was $130.3^{\circ}$. The VAS showed an improvement from 5.96 to 3.05 at the last follow up. IKDC subjective score and Lysholm score also showed an improvement from 46.5 to 64.5 and from 61.9s to 79.58 respectively. Tegner score was improved from 2.9 to 3.6. Overall, 61.5% of patients reported they were completely or mostly satisfied with procedure. There were no significant differences noted between lateral and medial groups as well as isolated and combined groups. In 2nd look arthroscopy, 10 of 18 cases showed good pheripheal healing and there were 6 cases of partial and 2 of complex tear. We observed graft subluxation or extrusion in 12 of 16 cases who were evaluated with follow-up MRI. Conclusion: Meniscus allograft transplantation alone or in combination with other procedure showed an improvement in knee pain and clinical score. But there were no significant difference between lateral and medial groups or isolated and combined procedure groups.

Sensitivity of Arctic warming to sea surface temperature distribution over melted sea-ice region in atmospheric general circulation model experiments

Jun, Sang-Yoon,Ho, Chang-Hoi,Kim, Baek-Min,Jeong, Jee-Hoon Springer-Verlag 2014 Climate dynamics Vol.42 No.3

HBV : The Fate of Chronic Hepatitis B in the Era of Antiviral Therapy

( Yoon Hea Park ),( Beom Kyung Kim ),( Jun Yong Park ),( Seung Up Kim ),( Do Young Kim ),( Ja Kyung Kim ),( Kwan Sik Lee ),( Kwang Hyub Han ),( Chae Yoon Chon ),( Sang Hoon Ahn ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Chronic hepatitis B (CHB) can progress to cirrhosis and hepatocellular carcinoma (HCC), either of which can lead to a liver-related death. The progression of liver disease in hepatitis B virus (HBV) infection is fostered by active virus replication. Recently, antiviral therapy with minimal side effects have become available to achieve sustained suppression of HBV replication, thereby preventing cirrhosis, hepatic failure, and, ultimately, HCC. The aim of this study is to reappraise the clinical courses regarding disease progression in the era of antiviral therapy for Korean CHB patients who were potential candidates for antiviral therapy. Methods: Between 2001 and 2005, treatment-naive CHB patients without cirrhosis were enrolled and followed-up for at least 5years. During follow-up period, patients have received antiviral therapy according to the Korean Association for the Study of the Liver guideline, if indicated. Ultrasonography and laboratory assessment were performed regularly. Primary endpoints were development of cirrhosis, or hepatic decompensation, HCC, or liver-related deaths, which were examined using Kaplan-Meier method. Results: Of 360 patients, 323 (89.7%) received antiviral therapy such as lamivudine (70.6%), entecavir (8.7%), or telbivudine (6.5%). During a median follow-up period of 94 months, cirrhosis developed in 29 (8.1%) patients, hepatic decompensation in 4 (1.1%) patients, and HCC in 15 (4.2%) patients. The annual incidence of cirrhosis, hepatic decompensation, and HCC were 1.05%, 0.14%, and 0.53% per person-year, respectively. Age was an independent prognostic factor for developing cirrhosis (hazard ratio [HR] 1.075; 95% confidence interval [CI] 1.037-1.116), whereas those for developing HCC were age (HR 1.060, 95% CI 1.012-1.111) and progression to cirrhosis (HR 17.470, 95% CI 5.081-60.063). Conclusions: In the era of antiviral therapy, the overall clinical courses of patients with CHB in Korea have been much improved since the introduction of lamivudine in 1999. However, older age and cirrhosis still remain risk factors for HCC.

Pulsed Plasma Process for the Flue Gas Removal from the Industrial Incinerator using Peak 200-kV, 10 kA Pulse Modulator

Sung-Duck Jang,Dong-Jun Koh,Jong-Seok Oh,Moo-Hyun Cho,Yoon-Gyu Son 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.44 No.52

The electrical discharge process, especially the pulsed plasma discharge process can be applied to the removal of pollutant gases from industrial plants such as power generation plants and incinerators. Up to now, most of the study has been performed in a laboratory scale with short-term tests due to the lack of a reliable pulse modulator with a high average power. The pulsed corona discharge method shows encouraging results for the removal of NOx and SO2 gases based on smallscale experiments. A 120-kW high average power modulator for industrial applications of the pulsed corona process to remove ue gas has been designed and manufactured. It is one of the largest scale modulator systems in the world for treating NOx and SO2 simultaneously. Its design specications are as follows: an average power of 120 kW, a peak voltage of 200 kV with a full width at half maximum (FWHM) of 500 nsec, a peak current of 10 kA, and a pulse repetition rate of 300 Hz. It is required to have long lifetime and high reliability for commercial plant application because the downtime for maintenance aects plant availability. A high-power, fast semiconductor switch, a magnetic-pulse-compression (MPC) switch, and a fast-pulse transformer are essential components to meet these requirements. The 120-kW high average power modulator has been installed and tested at an industrial incinerator plant with a gas ow of 50,000 Nm3/Hr. This modulator was operated with pulses of up to 150 kV with 500-nsec (FWHM) pulse widths at a 240-Hz repetition rate in a plasma reactor. This paper presents the design details and operational test results. Especially, the dynamic operating characteristics of the MPC modulator combined with the non-thermal plasma reactor were measured, and the SO2 and NOx removal characteristics were analyzed.

Effect of Epidermal Growth Factor on the Fertilization and Development of In Vitro - Matured Porcine Oocytes

Sang Jun Uhm,Eun Young Kim,Myo Kyung Kim,San Hyun Yoon,Se Pill Park,Kil Saeng Chung,Jin Ho Lim 한국동물자원과학회(구 한국축산학회) 1996 한국축산학회 심포지움자료집 Vol.- No.-

Synthesis and Stereochemistry of the Complexes of Cobalt(III) with New Tetradentate Ligands. Cobalt(III) Complexes of Ethylenediamine-N,N'-di-${\alpha}$-butyric Acid

Jun, Moo-Jin,Han, Chang-Yoon,Park, Yoon-Bong,Choi, Sung Rack Korean Chemical Society 1985 Bulletin of the Korean Chemical Society Vol.6 No.3

A new flexible $N_2O_2$-type tetradentate ligand, ethylene-diamine-N,N'-di-${\alpha}$-butyric acid (eddb), has been synthesized, and a series of cobalt (III) complexes of eddb, $[Co(edda)(L)]^{n+}$ (L = $Cl_2$, $(H_2O)_2$, $Cl\;H_2O$, and $Co^2_3$), have been prepared. Only s-cis isomers have been yielded during the preparation of complexes. Ring strain is cited as the primary cause for the preference for the s-cis geometric configuration.