工業敎育大學의 實驗實習施設 共同活用 및 運營에 關한 硏究

尹錫天,牟相榮,權五憲,千炳善,周赫鍾,李永臣,金健中,羅聲雄,洪錫雨,吳德成,盧泰天 충남대학교 공업교육연구소 1983 論文集 Vol.6 No.2(A)

The College of Engineering was specialized to have a sole function for supplying technical high school teachers in 1977 and referred to as the College of Industrial Education according to the Government's policy. The economic impact mainly due to the oil-crises caused a severe problem to this college, namely discrepancy of supply to demand scale. Accordingly the number of student enrollment was to be fluctuated from 479 in 1979 through 800 in 1979 and 900 in 1980 to 430 in 1981. And the engineering departments have been attached to this Engineering Education College since 1981. These circumstanses met singnificant inefficiency in facility operation. This program aims at providing an overall relocation of the college facilities and preparing the practical system to make the joint-operation possible taking into account circulation, academic stream, budget, maintenance, etc. However this study could not get optimal spacing but some trade-off, considering the current situation.

Case report : Immunoglobulin G4-Related Disease Mimicking Unresectable Gallbladder Cancer

( Yoon Suk Lee ),( Sang Hyub Lee ),( Min Geun Lee ),( Seung June Lee ),( Jin Hyeok Hwang ),( Eun Shin ),( Yoon Jin Lee ) The Editorial Office of Gut and Liver 2013 Gut and Liver Vol.7 No.5

Immunoglobulin G4 (IgG4)-related disease is a novel dis-ease entity that can involve diverse organs, causing specific diseases, including autoimmune pancreatitis, sclerosing cholangitis, cholecystitis, inflammatory aortic aneurysm, and inflammatory pseudotumor. IgG4-related disease is charac-terized by elevated serum IgG4 concentrations, abundant IgG4 lymphoplasmacytic infiltration, and dramatic steroid responses. It is clinically important to differentiate this rare disease from primary sclerosing cholangitis and cholangio-carcinoma, because the treatment and prognosis of these two diseases are completely different. However, the preoper-ative diagnosis is challenging, and the disease is frequently misdiagnosed. If the serum level of IgG4 is within the normal range, the diagnosis of IgG4-related disease is more difficult. This article reports on a 59-year-old man with IgG4-related disease mimicking unresectable gallbladder cancer with nor-mal serum IgG4 concentrations. (Gut Liver 2013;7:616-620)

자궁경부 상피내 종양과 침윤성 자궁경부암에서의 FHIT 단백 발현 소실

이봉규(Bong Gyu Lee),이종섭(Jong Seob Lee),이동혁(Dong Hyeok Lee),김미진(Mi Jin Kim),이영기(Young Gi Lee),박윤기(Yoon Kee Park),이두진(Doo Jin Lee),이승호(Sung Ho Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.2

목적 : 자궁경부 전암성 병변과 침윤성 자궁경부암에서 Fhit 유전자의 소실 정도가 침윤성 자궁경부암의 다른 예후 인자와 어떤 관련이 있는지, 그리고 침윤성 자궁경부암으로의 진행을 조기에 예견하는데 도움이 되는지를 알아보고자 하였다. 연구 방법 : 1997년 6월부터 2001년 2월까지 영남대학교 의과대학부속병원에서 자궁경부 전암성 병변 및 침윤성 자궁경부암으로 진단되어 치료를 받은 환자 91예의 파라핀 병리 표본을 이용, 절편을 만들어 탈파라핀과 함수과정을 거쳐 3% 과산화수소에서 작용시킨 후 microwave oven에서 처리하여 항원 노출을 극대화시켰다. 그 후 과산화 수소를 작용시켜 내인성 과산화 효소를 억제시킨 후 TBS 완충액으로 수세한 다음 단백질 차단제 (Zymed, USA)를 도포한 후 일차항체를 반응시켰다. Fhit 다클론 항체 (Zymed, USA)는 1 : 300으로 희석하여 상온에서 1시간 30분 동안 반응시킨 뒤 TBS로 수세하고 이차항체인 link antibody (LSAB kit DAKO, USA)를 37℃에서 30분간 부착시킨 후 TBS 완충액으로 3회 세척하고 Strepavidin-HRP (Zymed, USA)로 15분간 도포 하였다. 다시 TBS 완충액으로 세척한 다음 AEC로 발색시켜 10% Mayer's hematoxyline으로 대조 염색하여 세포질에 과립상의 진한 갈색으로 염색된 것을 양성으로 판독하였다. 면역 조직 염색된 표본은 염색의 강약과 염색된 부위의 넓이에 따라 점수를 준 후 서로 곱한 점수를 최종 점수로 하였는데 이를 편의상 Fhit 단백 발현 점수 (Fhit protein expression score)라 하였다. Denise 등 (2000)의 방법에 따라 Fhit 염색 점수가 1-3점은 Fhit 단백 발현이 소실되었다고 보았으며 4-9점은 Fhit 단백 발현이 있다고 보았다. 통계 처리는 x2test, Fisher's exact test를 이용하였다. 결과 : HSIL 33예 중 15예 (45.5%)가 Fhit 단백 발현 소실이 있었고 이는 LSIL의 Fhit 단백 발현 소실율 29.0%에 비해 높은 경향이 있었으나 통계적으로 유의한 차이는 없었다. 침윤성 편평 상피세포 자궁경부암의 77.8%에서 Fhit 단백 발현 소실이 있었고 이는 HSIL의 Fhit 단백 소실율 (45.5%)에 비해 유의하게 높았다. 세포 형태, 자궁경부암의 병기. 임파절 전이 유무, 암표지자, 방사선 치료 유무 등과 Fhit 단백 발현 소실 사이에는 서로 연관이 없었다. 그러나 종양의 크기에서는 종양의 크기가 4 cm 이하인 경우에서, 항암화학 요법에서는 항암화학 요법을 추가로 실시하지 않은 군에서, 침윤성 자궁경부암의 재발 유무에서는 재발하지 않은 군에서 각각 Fhit 단백 발현 소실이 유의하게 많았고 침윤성 자궁경부암이 재발한 군의 재발까지의 기간 (중앙값)도 Fhit 단백 발현 소실이 많았던 군에서 오히려 유의하게 길었다. 결론 : Fhit 단백 발현 소실 정도는 자궁 경부 전암성 병변 사이에는 서로 유의한 차이가 없었고 침윤성 자궁경부암의 경우 전암성 병변에 비해 유의하게 Fhit 단백 발현이 낮아 이들의 진단이나 침윤성 암으로의 진행을 아는데 도움이 될 것이나 Fhit 단백 발현 소실 정도를 침윤성 자궁경부암의 예후 인자로 이용하기에는 앞으로 더 많은 연구가 필요하고 보다 더 객관적이고 구체적인 Fhit 단백 발현 점수 측정법이 개발되어야 할 것으로 생각한다. Objective : The author evaluated the relationship between the loss of Fhit and prognostic factors of invasive cervical carcinomas. Methods : The formalin prepared tissue specimens of 64 CINs and 27 invasive cervical carcinomas patients diagnosed and treated at the department of obstetrics and gynecology, Yeungnam university hospital from June 1977 to February 2001 were obtained. And then the tissue specimens were analysed & measured by immunohistochemical methods. Results : The loss of Fhit was significantly reduced in invasive cervical carcinomas compared to CINs. But we found there is no relationships between the loss of Fhit and prognostic factors, stage, tumor marker, lymphatic spread and radiation therapy of invasive cervical carcinomas. Conclusions : Although the loss of Fhit was not related to the precancerous lesion of cervix, we could confirm that the loss of Fhit was important in the progression of precancerous lesions to invasive cervical carcinomas.

[PB-0050] Diversity Analysis of the Korean Codonopsis lanceolata by the Comparison of Chloroplast Genomes

Namsu Jo(Namsu Jo),Ju Hyeok Kim(Ju Hyeok Kim),Jinsu Gil(Jinsu Gil),Sungcheol Koo(Sungcheol Koo),Mi Sun Lee(Mi Sun Lee),Eunjee Lee(Eunjee Lee),Yoon A Kim(Yoon A Kim),Yi Lee(Yi Lee) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

The Korean Society of Gastroenterology& SLDDS 2031 : Slide Session ; K-BP-21 : Pancreatobiliary ; The Properties of Endoscopic Ultrasonography Compared with CT and MRI Regarding the Size Measurement of Pancreatic Cyst Lesion

( Yoon Suk Lee ),( Kyu Hyun Paik ),( Hyung Woo Kim ),( Jong Chan Lee ),( Jai Hwan Kim ),( Jin Hyeok Hwang ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Consensus regarding which modality is optimal in size measurement was not achieved although cyst size and growth are important for clinical decision. This study aimed to evaluate the properties of size measurement in each modality (EUS, CT and MRI) for pancreatic cyst lesion. Methods: Patients were selected when measured by all three imaging studies within 3 months before resection for pancreatic cystic lesion at tertiary teaching hospital. The cyst size measured by each imaging modality was compared with pathologic size as a reference standard using Bland-Altman analysis and intra-class correlation coeffi cients (ICCs). The difference with respect to locations (head, body and tail) and cyst size was also analyzed with linear regression analysis. Results: A total of 35 patients was enrolled retrospectively. The mean size difference (absolute size determined by image minus size determined by pathology) was 8.17 mm (ICC 0.861) in EUS, 8.29 mm (ICC 0.947) in CT and 9.74 mm (ICC 0.929) in MRI. Bland-Altman analysis showed that the limits of agreement were -17.26 to 21.38 mm in EUS, -5.99 to +20.73 mm in CT, and -6.65 to + 23.85 in MRI. And the size by EUS tended to be read smaller in tail portion, while those by CT and MRI did not. With the increase of cyst size, the discrepancy decreased in EUS. However, when the cyst size was more than about 4 cm, the size in EUS was estimated to be smaller than those in pathology using linear regression analysis (p = 0.004, r = 0.474). Conclusions: CT had the highest agreement with pathology although EUS and MRI showed the ICC of >0.8. And cyst size and cyst location of pancreas tail were predictors for the discrepancy in EUS.

Systems Level Metabolic Engineering of Escherichia coli for Enhanced Production of LPhenylalanine

Yoon Hyeok LEE,Hyun Bae BANG,Ki Jun JEONG 한국생물공학회 2015 한국생물공학회 학술대회 Vol.2015 No.10

Drug repositioning for enzyme modulator based on human metabolite-likeness

Lee, Yoon Hyeok,Choi, Hojae,Park, Seongyong,Lee, Boah,Yi, Gwan-Su BioMed Central 2017 BMC bioinformatics Vol.18 No.suppl7

<P><B>Background</B></P><P>Recently, the metabolite-likeness of the drug space has emerged and has opened a new possibility for exploring human metabolite-like candidates in drug discovery. However, the applicability of metabolite-likeness in drug discovery has been largely unexplored. Moreover, there are no reports on its applications for the repositioning of drugs to possible enzyme modulators, although enzyme-drug relations could be directly inferred from the similarity relationships between enzyme’s metabolites and drugs.</P><P><B>Methods</B></P><P>We constructed a drug-metabolite structural similarity matrix, which contains 1,861 FDA-approved drugs and 1,110 human intermediary metabolites scored with the Tanimoto similarity. To verify the metabolite-likeness measure for drug repositioning, we analyzed 17 known antimetabolite drugs that resemble the innate metabolites of their eleven target enzymes as the gold standard positives. Highly scored drugs were selected as possible modulators of enzymes for their corresponding metabolites. Then, we assessed the performance of metabolite-likeness with a receiver operating characteristic analysis and compared it with other drug-target prediction methods. We set the similarity threshold for drug repositioning candidates of new enzyme modulators based on maximization of the Youden’s index. We also carried out literature surveys for supporting the drug repositioning results based on the metabolite-likeness.</P><P><B>Results</B></P><P>In this paper, we applied metabolite-likeness to repurpose FDA-approved drugs to disease-associated enzyme modulators that resemble human innate metabolites. All antimetabolite drugs were mapped with their known 11 target enzymes with statistically significant similarity values to the corresponding metabolites. The comparison with other drug-target prediction methods showed the higher performance of metabolite-likeness for predicting enzyme modulators. After that, the drugs scored higher than similarity score of 0.654 were selected as possible modulators of enzymes for their corresponding metabolites. In addition, we showed that drug repositioning results of 10 enzymes were concordant with the literature evidence.</P><P><B>Conclusions</B></P><P>This study introduced a method to predict the repositioning of known drugs to possible modulators of disease associated enzymes using human metabolite-likeness. We demonstrated that this approach works correctly with known antimetabolite drugs and showed that the proposed method has better performance compared to other drug target prediction methods in terms of enzyme modulators prediction. This study as a proof-of-concept showed how to apply metabolite-likeness to drug repositioning as well as potential in further expansion as we acquire more disease associated metabolite-target protein relations.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12859-017-1637-5) contains supplementary material, which is available to authorized users.</P>

HCC : PE-074 ; A case of synchronous hepatocellular carcinoma and renal cell carcinoma treated with radio-frequency ablation

( Yoon Serk Lee ),( Jeong Han Kim ),( Hyeon Young Yoon ),( Soon Young Ko ),( Won Hyeok Choe ),( So Young Kwon ),( Chang Hong Lee ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Radio-frequency ablation (RFA) is curative treatment for hepatocellular carcinoma (HCC). Recently, RFA is also known to be an effective treatment option for renal cell carcinoma (RCC) less than 4 cm. Case: We experienced a case of 53-year-old male who was referred to konkuk university hospital because of suggestive HCC, RCC, and alcoholic liver cirrhosis (Child-Pugh score 9, stage B). Contrast-enhanced abdomen CT and MRI images showed liver cirrhosis with 2.8 cm ill-defined mass in segment 2 of the liver and 2.4 cm hypervascular mass in left kidney. These findings were compatible with double primary cancers of HCC and RCC. There was no evidence of lymph node or distant metastasis. Serum AFP was 6.93 ng/mL (<7.0 ng/mL). HBsAg, Anti-HBs, Anti-HCV were all negative. Transarterial chemoembolization (TACE) was performed to treat the HCC. After TACE, focal lipiodol uptake defect was noticed on follow up CT images and loco-regional treatment was recommended. Therefore, we performed RFAs to treat HCC and RCC. There was no significant complication after the procedure. One month later, follow-up CT images were taken. There was no evidence of recurrence. Conclusions: We report a case of synchronous HCC and RCC treated with RFA.

Case Report : A synchronous hepatocellular carcinoma and renal cell carcinoma treated with radio frequency ablation

( Yoon Serk Lee ),( Jeong Han Kim ),( Hyeon Young Yoon ),( Won Hyeok Choe ),( So Young Kwon ),( Chang Hong Lee ) 대한간학회 2014 Clinical and Molecular Hepatology(대한간학회지) Vol.20 No.3

Radio-frequency ablation (RFA) is a curative treatment for hepatocellular carcinoma (HCC). Percutaneous RFA has been shown to be beneficial for patients with small renal cell carcinoma (RCC) lacking indications for resection. We experienced the case of a 53-year-old male who had conditions that suggested HCC, RCC, and alcoholic liver cirrhosis. Abdominal contrast-enhanced computed tomography (CT) and magnetic resonance image showed liver cirrhosis with 2.8 cm ill-defined mass in segment 2 of the liver and 1.9 cm hypervascular mass in the left kidney. These findings were compatible with the double primary cancers of HCC and RCC. Transarterial chemoembolization (TACE) was performed to treat the HCC. After the TACE, a focal lipiodol uptake defect was noticed on a follow up CT images and loco-regional treatment was recommended. Therefore, we performed RFAs to treat HCC and RCC. There was no evidence of recurrence in the follow up image after 1 month. (Clin Mol Hepatol 2014;20:306-309)

Developing emission factors for dairy cow enteric fermentation in Korea

Lee, Joo Young,Lee, Min Hyeok,Lee, Jong Seok,Chun, Yoon-Young,Kim, Kyoung Hoon,Kim, Min Seok,Lee, Kun Mo Elsevier 2018 Journal of cleaner production Vol.198 No.-

<P><B>Abstract</B></P> <P>We developed emission factors for dairy cow enteric fermentation in Korea, along with their uncertainties. A total of 30 dairy cow farms were randomly chosen from the 3500 possible farms, then data on the number of heads, their body weights, the amount of feed intake, and the feed composition were collected. Statistical analysis of the methane conversion factor (Ym) and gross energy (GE) data showed that the emission factor for the enteric fermentation of a cow should be estimated using three different body weight classes (equivalent to the growth phases). The EF values for the three classes, A, B, and C in this study were greater than those recommended by the 2006 IPCC guideline by 2.3%, 78.5%, and 7.6%, respectively. The Monte Carlo simulation (MCS) and bootstrap method were used to estimate emission factor uncertainty, and the results showed that the bootstrap method gave smaller confidence interval (CI) width and a smaller percentage uncertainty (U). Treating Ym as constant leads to underestimation of the uncertainty of the emission factors, compared to treating Ym as a random variable. Thus, estimation of the emission factors and their uncertainties should be based on an emission factor calculation model where both Ym and GE are treated as random variables.</P>