SOD2와 NDPK2 유전자 집적에 의한 페튜니아의 아황산가스 저항성 증진

이수영(Su Young Lee),천경성(Kyeong-Seong Cheon),김소영(So Young Kim),권오현(O Hyeon Kwon),이혜진(Hye Jin Lee),김원희(Won Hee Kim),유봉식(Bong Sik Yoo) 한국원예학회 2016 원예과학기술지 Vol.34 No.1

유전자 집적에 의해 아황산가스 저항성 증진 페튜니아를 개발하고자 비생물적스트레스 저항성 유전자로 널리 알려진 SOD2 와 NDPK2유전자가 각각 도입된 SOD2 형질전환 계통 SOD2-2-1-1-35(T₄)[S(T₄)]와 NDPK2 형질전환 계통 NDPK2-7-1(T₂)[N7-1(T₂)]간 상호교잡을 실시하여 교잡 제1세대를 획득하였다. 교잡 제1세대 중 SOD2와 NDPK2유전자가 모두 집적된 개체 획득율은 32.1-73.0%이었고, SOD2와 NDPK2유전자가 모두 집적된 개체는 SOD2 또는 NDPK2유전자가 단독 도입된 개체에 비해 아황산가스 30ppm처리 피해율이 2.6-5.1배 낮아 아황산가스 저항성이 증진되었음을 확인하였다. 또한 아황산가스 저항성이 증진된 교잡 제1세대를 자가수분에 의해 세대진전 시켜 획득한 교잡 제2세대의 아황산가스 저항성 검정을 통하여 교잡 제1세대에서의 아황산가스 저항성이 후대에서도 안정적으로 발현함을 확인하였다. 또한, 광합성 효율 증진 조사를 통해 아황산가스 저항성이 증진된 SOD2와 NDPK2유전자 집적 후대가 아황산 가스에 대한 스트레스를 덜 받고 있음을 확인하였고, RT-qPCR분석을 통해 SOD2와 NDPK2유전자 집적 후대의 아황산가스 저항성 증진이 SOD2와 NDPK2 유전자 집적에 의한 효과임을 확인하였다. The aim of this study was to develop a transgenic petunia with enhanced resistance to sulfur dioxide (SO₂) gas by stacking two genes, SOD2 and NDPK2, which are both known to confer resistance to abiotic stresses. The first-generation hybrids (TF1) were obtained through reciprocal crosses between an SOD2-transgenic line SOD2-2-1-1-35(T₄)[S(T₄)] and an NDPK2- transgenic line NDPK2-7-1(T₂)[N7-1(T₂)]. Approximately 32.1-73.0% of the first-generation hybrids (TF₁) carried both SOD2 and NDPK2 genes. These hybrids showed 2.6 and 5.1 times less damage than hybrids carrying only SOD2 or NDPK2 genes, respectively, when they were treated with SO₂ gas at 30 ppm. This confirmed that the heterozygous hybrids were more resistant to SO₂ than the hybrids carrying either one of the resistance genes. Second-generation hybrids (TF₂) were obtained by selfing the TF₁ individuals. We confirmed the expression of the stacked genes in the TF₂ hybrids by phenotypic observation of their response to SO₂ gas at 30 ppm as well as using RT-qPCR and photosynthetic efficiency.

일 대학병원에 입원한 노년기 양극성 장애 환자의 인구학적 및 임상적 특징

조숙현(Sook Hyun Cho),정한용(Han Yong Jung),권영준(Young Jun Kwon),이소영(So Young Lee),김양래(Yang Rae Kim),김윤정(Yun Jung Kim) 대한노인정신의학회 2006 노인정신의학 Vol.10 No.2

Objectives : As the elderly population is continuing to increase, psychiatric diseases of the elderly are becoming an important social issue. This study looks into the demographical and clinical features of the elderly patients diagnosed with bipolar disorder in a University hospital. Methods : The study subjects include patients admissioned in the closed wards of Soonchunhyang Seoul hospital and Cheonan hospital from March, 2000 to February, 2005 who met the DSM-IV-TR criteria for bipolar disorder at the time of discharge. A total of 146 patients (76 men, 70 women) medical records were studied retrospectively. Subjects older than the age of 50 at time of admission were grouped as the old age group, whereas those who were younger than 50 as the young age group. Also among the old age group, those who had the first onset of episode under the age of 50 were grouped as the early onset group, whereas those who had the first onset after the age 50 were grouped as the late onset group. Results : The number of bipolar disorder patients in the young age group and old age group were 73 and 73 respectively. The number of early onset group and late onset group were 46 and 23 respectively. The old age group had a relatively higher incidence of bipolar II disorder than the young age group. Also in the old age group there was a higher incidence of hypomanic or depressive episode rather than manic episode compared to the young age group. And in the old age group psychotic symptoms were less common and the mean admission length shorter than the young age group. Additionally in the old age group treatment with Lithium monotherapy or with no mood stabilizers at all were relatively common. Among the old age group, late onset group had a higher rate of bipolar II disorder than the early onset group. Conclusion : This study shows differences in the demographical and clinical features among different age groups. But additional research would be required to determine whether the bipolar disorders in the elderly or late onset group are actually a different type of disorder from those of the young.

혈관형성 조절물질 탐색을 위한 제브라피쉬 동물모델의 이용

소주훈,최태영,권병목,김철희 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2

The zebrafish (Danio rerio) is now the prominent vertebrate model system for clarification of the roles of specific genes and signaling pathways in development. Zebrafish embryos provide many advantages for experimental and genetic analysis of vascular development. In this study we describe the use of zebrafish model system for the experimental study of angiogenesis. We established several technical methods to define cellular and molecular alterations in vascular development; whole-mount in situ hybridization, microangiography, and alkaline phosphatase staining. We also introduced the overexpression experiments by using in vitro-transcribed synthetic mRNAs and vascular-specific gene promoters. For the gene knock-down analyses, we challenged morpholino antisense oligonucleotide system. With these efforts, the zebrafish might be used as a powerful tool for the screening of effective angiogenic factors.

Strontium Barium Niobate 단결정에서 광굴절 부피격자를 이용한 동적 광편향

소지영,이권연 순천대학교 공업기술연구소 1997 工業技術硏究所論文集 Vol.11 No.-

In this paper, we report the results of a theoretical and experimental investigation of a dynamic photorefractive deflector. This device is based on the diffraction from a dynamic volume grating photoinduced in Cedoped strontium barium niobate(Sr_0.6Bα_0.4Nb_2O_6) single crystal by the interference of two writing beams. The diffraction properties of dynamic volume gratings, including the effect of additional two - beam coupling, are also considered.

바나듐과 크롬의 쿠페론 착물의 전기화학적 행동의 차이

권영순,이상미,정미영,박소영 가톨릭대학교 자연과학연구소 2004 자연과학논문집 Vol.25 No.-

바나듐과 크롬의 쿠페론 착물의 전기화학적 행동을 아세트산 완충용액(pH 5.0)과 붕산염 완충용액(pH 9.5)에서 조사해 본 결과 크롬-쿠페론 착물의 환원봉우리전위(E_(ct)^(p))가 쿠페론의 봉우리전위(E₁^(p))보다 작다 (E_(ct)^(P) < E₁^(P)). 붕산염 완충용액에서 크롬-쿠페론계의 전도된 봉우리가 크게 나타나는 것은 E_(ct)^(P)와 E₁^(P)의 차이가 큰 때문인 것으로 생각된다 (ΔE = 0.40V). When the peak potentials of V-cupferron and Cr-cupferron complex compare at both buffer solution(pH 5.0 and pH 9.5), the peak potential of Cr-cupferron(E_(ct)^(P)) is smaller the peak potential of cupferron (E₁^(P)) at both buffer solution (E_(ct)^(p)) < E₁^(p)). The appearance of larger inverted peak of Cr-cupferron in borate buffer solution results from large difference of E_(ct)^(p) and E₁^(p) (ΔE=0.40 V).

이동통신망에서의 최적 셀 할당에 관한 연구

권수태,정소영,윤상흠,김호준 전주대학교 공학기술종합연구소 2003 전주대학교 공학기술종합연구소 학술논문집 Vol.9 No.1

The paper considers a cell allocation problem in the Mobile Wireless Communication Network with limited capacity of Base Switching Center(BSC) and Mobile Switching Center(MSC), and the aim is assigning cells to BSC and MSC for supporting an efficient handoff and reducing the cabling cost. The cell assignment problem is formulated as a mixed integer programming, and a genetic algorithm is developed to derive the optimal solution. A verity of numerical problems are presented and then some interesting properties are exploited that are useful for charactering the problem.

청주시 소비자의 그린마케팅에 대한 인식과 그린제품 구매의도

권소영, 박혜영, 최혜미, 최현미, 유현정 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The purpose of this study was to analyze consumers' perception of green marketing and purchase intention of green products. Questionnaire survey was conducted from 9 August to 29 August 2010. We have surveyed 298 consumers who were living in Cheongju area. Major findings are as follows. First, consumers' environment perception and involvement, awareness of green marketing, evaluation for green products, purchase intention of green products were generally positive. It means that Korean consumers have a positive attitude and behavioral intention for green consumption. Second, consumers responded high perception level for necessity of green marketing and purchase intention of green products. On the other hand, consumers think that green product manufacturers need to more protect our environment. Consumers evaluated the quality of green products is good but its price is high. Third, insufficient information was the most common response of the items asked for the resistance of the whole process for the production of green products. For benefit part, high quality and symbolic value of green consumer were given answer highly. Fourth, there were differences among the consumer groups from environment perception, awareness of green marketing, evaluation for green products, and purchase intention of green products by consumers' characteristics. Old aged group were high involved on environment and student group showed relatively low environment perception and involvement of green consumption. Four million won or less income groups were relatively higher interest in green products and eco-conscious consumer groups have a high degree of purchasing intention of green products.

인터넷 쇼핑몰 이용자의 촉각단서 욕구가 의류상품 구매행동에 미치는 영향에 관한 연구 : 구매경험, 지각된 위험의 조절효과 An Moderating Effect of Perceived Risk, Purchased Experience

권소영,이진영,오희선,서용한 한국의류산업학회 2004 한국의류산업학회지 Vol.6 No.5

This study is to explore how consumers' needs for tactile cues affect their purchasing behaviors in the internet shopping mall. Since previous studies about internet shopping malls are mainly performed on the primary factors of perceived risk, there are insufficient studies of tactile cues for apparel products. Emphasis of this study is placed on verifying the following hypothesis; it is expected that consumers' needs for tactile cues affect apparel purchasing behaviors in inter-net shopping. The questionnaire was administered to 20 to 30 year old male and female respondents who are either students or businessmen living in the Busan area. 150 questionnaires were completed and collected for data analysis. The data were, using SPSS 10. 0 for Window, statistically analyzed by frequency and factor analysis for VARIMAX, Cronbach's coefficient and Linear regression analysis. Data were, using SPSS 10. 0 for Window, statistically analyzed by frequency and factor analysis for VARIMAX, Cronbach's coefficient, and ANOVA. The results of data analysis are as follows: First, the tactile cues negatively affect purchase intentions of consumers. This shows that apparel internet shoppers who have high desire for tactical cues tend to avoid purchasing products through the internet. Second, the factor analysis of the moderating effect on perceived risks shows that the perceived risks significantly moderate both the tactile cues and purchase intention of consumers. Third, analysis of purchased experience also shows that purchased experiences significantly moderate both the tactile cues and purchase intention.

개에서 발생한 양성 및 악성신경집종 증례에 대한 면역조직화학적 진단

염소영, 신상경, 김태왕, 배대권, 김근형, 안병우 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.1

본문참고

벗김전압전류법을 이용한 미량의 루테늄의 정량

權英順,金素眞 카톨릭대학교 성심교정 1995 論文集 Vol.2 No.-

루테늄을 정량하기 위하여 벗김전압전류법을 사용하였다. 히드라진-초산용액에서 촉매효과와 측적방법을 결합하여 실험한 검출한계는 7.0x10-9M이다. 이때의 조건은 초산완충용액 0.01M, 히드라진0.01M, 축적전위 -0.76V, 축적시간 2.0분이다. Stripping voltammetric for the determination of Ruthenium is described. By the use of combined catalytic effects and accumulation in hydragine-acetate solution, the limit of detection is 7.0x10-9M. Optimized condition are 0.01M acetate buffer solution containing 0.01 M hydragine, an accumulation potential of -0.76V(vs. As/AgCl) and accumulation time of 2.0 min.