Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

확장된 계획행동이론을 통한 무주 태권도공원 방문객의 방문의도 분석

박용수(Yong Soo Park),한진욱(Jin Wook Han),김민수(Min Soo Kim) 한국사회체육학회 2012 한국사회체육학회지 Vol.0 No.48

The purpose of this study was to predict travelers` behavioral intention to visit the Muju Taekwondo Park, based on extended Theory of Planned Behavior(TPB). Tourists` motivation was newly included in determining their behavioral intention to visit the park along with existing three components of traditional Theory of Planned Behavior(attitudes, subjective norms, and perceived behavioral control). To this end, a total of 241 Taekwondo masters was selected using a conveniently sampling method and responded to a survey questionnaire. The 206 usable questionnaires were utilized to the final data analyses. Statistical Package of PASW 15.0 was used for data analyses including descriptive analysis, exploratory factor analysis, reliability analysis, correlation analysis, and multiple regression analysis. The results of this study were as follows. Firstly, attitudes toward visiting the park had a significant impact on visiting the park Secondly, subjective norms had a significant influence on visiting the park. Thirdly, perceived behavioral control significantly affected behavioral intention to visit the park. Lastly, motivation was a significant predictor of subjects` visiting the park. Additionally, practical implications of the findings and suggestions were discussed.

청도새마을운동 기념공원 기본계획

권진욱 ( Jin Wook Kwon ),박찬용 ( Chan Yong Park ) 한국농촌계획학회 2014 농촌계획 Vol.20 No.3

The Saemaeul Movement, which is the representative national campaign of Korea aimed at the development of local communities, has drawn a great deal of attention from home and abroad and formed an element of Korea``s national brand since 2000. Accordingly, this research was conducted for the purpose of constructing a memorial park in Shindo Village, Cheongdo County, North Kyoungsang Province, which is a home to the Saemaeul Movement. As the Saemaeul Movement is benchmarked by many countries around the world today, this research aims to communicate the spirit and social value of the movement and disseminate its effects of local community development in rural areas through the construction of a memorial park. In this study, the design motive of the memorial park was conceived through the historical review and case studies of the Saemaeul Movement. In parallel, theoretical study was also conducted on design techniques as the basis of this research. In consideration of the characteristics of a technical article, this research was conducted in several phases. In the first phase, the conditions of the site where the park construction was planned were analyzed and the direction of its development was set. In the second phase, the main theme and the basic principles of planning were established, and the contents of the park construction project were devised in detail. In the last phase, a comprehensive plan was established, including a space layout to accommodate activities, facilities and programs to be introduced to the park. The park construction site (106,000㎡) was divided into four zones (memorial zone, historical theme park, education zone and experience zone) based on circulation planning aimed at creating memorial space, and was linked to eco-friendly ecological space in consideration of environmental features. At a time when the Saemaeul Movement is being propagated across the world, the result of this study will help create a place for its memorial and play a pivotal role to boost international movements aimed at promoting co-prosperity across the global village. It will also bear significance as an example of theme-based park construction in a rural area and the invigoration of a local community.

청송 사과체험테마파크 기본계획

권진욱 ( Jin Wook Kwon ),박찬용 ( Chan Yong Park ) 한국농촌계획학회 2013 농촌계획 Vol.19 No.3

This study aimed to plan a distinctive apple theme park, thereby specializing the nationwide brand of Cheongsong apple. Detailed objectives included: to establish the best possible environments in Korea to taste and appreciate apple and enjoy the Cheongsong Apple Festival; to identify and foster natural, cultural and social resources in the clean environment of Cheongsong; to clusterize research and production infrastructures for strengthening local competitiveness; and to develop a hub for the vitalization of the region where visitors and locals can mutually prosper. The study was multi-phased. The first stage included basic surveys such as local status and environment analysis and similar case studies, and the second stage was to review the appropriateness of theme selection, develop basic principles and strategies for development goals and review and incorporate project details. And the third stage aimed to develop a comprehensive plan from spatial plans and program plans and suggest plans to vitalize the operation of the park. The dimension of the subject site was 180,150㎡, which was divided into four areas, in consideration of the land use and the environmental characteristics of the resources, for developing a land use scheme. The four areas were named: the apple-theme cultual area; the agricultural culture experience area; the plaza for exchange and harmony; and the plaza for natural observation. This study has significance in that it can serve as a case to develop farm theme parks, and as a case of appropriate development of programs to identify amenity resources with a focus on the existing resources and in consideration of local characteristics.

도시공원 이용자들의 형태 분석 및 이용 만족도에 관한 연구

박장근(Jang Geun Park),임란희(Ran Hee Lim),지용범(Yong Beom Ji),신희진(Hee Jin Shin) 한국여가레크리에이션학회 2001 한국여가레크리에이션학회지 Vol.21 No.-

The purpose of this study was to investigate of form analysis and extent of Satisfaction of people using park. For this study, the population was the user Mansuck Park in Suwon City from 06.06.2001 to 06.07.2001. Sampling was made according to the self-administration method The participants in the questionnaire were 224. From the analysis of data, this study reached the following coclusions. 1. An analysis of form ; 1) A walking was 53.1 percent according to the analysis of using form in traffic means. There were some differences by age, job, education level, income. 2) The evening was 41.5 percent , and the afternoon was 28.6 percent according to the time period of using. There were some differences in all items as gender, age, job, education level, income. 3) The family or relative was 48.2 percent, and the friends (lover) was 32.6 percent according to the companion. There were some differences in all items as gender, age, job, education level, income. 4) The rest and walk 50.0 percent , and meeting, talk was 17.4 percent according to the using park. There were some differences by gender, age, job, income. 5) The shade of a tree, bench, grass was 55.4 percent, and the promenade was 23.7 percent according to the place and facilities for using. There were some differences by age. 2 And extent of satisfaction for using park ; The satisfaction in the location of facilities, the management and scene of the park was in high content degree. But, the satisfaction in the number of facilities and the staff was in low content degree.

월악산국립공원의 곤충다양성에 관한 연구 : 노린재목, 딱정벌레목, 나비목

박성준(Seong-Joon Park),전용락(Yong-Lak Jeon),홍의정(Eui-Jung Hong),윤주창(Ju-Chang Yun),박서경(Seo-Kyoung Park),김병진(Byung-Jin Kim) 국립공원연구원 2010 국립공원연구지 Vol.1 No.3

본 연구는 2007년부터 2009년까지 3년 동안 월악산 모니터링 조사 지점과 동일지구에 대해 정기적인 주간조사를 통해 주간곤충류(노린재목, 딱정벌레목, 나비목)의 종목록을 확보 하여, 월악산국립공원의 생태계에 자연적 혹은 인위적으로 변화된 내용을 측정하여 곤충자원의 체계적인 관리를 위한 자료를 제공하고자 실시되었다. 본 조사기간 동안 채집 확인된 주간곤충류는 65과 404종(노린재목 21과 86종, 딱정벌레목 38과 244종, 나비목 6과 74종)이 조사되었다. 따라서 문헌조사를 포함한 결과 월악산국립공원에 서식하는 것으로 확인된 주간곤충류는 멸종위기야생동물 2종, 고유종 6종, 국외반출승인대상종 10종과 특정종 50종을 포함하여 총 72과 609종(딱정벌레목 44과 368종, 노린재목 22과 126종, 나비목 6과 115종)으로 나타났다. 아울러 조사지점별 분석결과 S3에서 종다양성이 가장 높게 나타났으며, S10은 다른 조사지점과 다르게 종다양성이 현저하게 떨어지는 것으로 나타났다. 각 조사기간별우점종으로는 2007년과 2009년도에 넉점박이송장벌레, 2008년도에는 더듬이긴노린재로 나타났다. This study were conducted for three years from 2007 to 2009 and at the same points as monitoring surveys, through regular surveys insects (Hemiptera, Coleoptera, Lepidoptera) to obtain the list, to measure and assess Woraksan National Park ecosystem’s natural or artificial change, to provide data for systematic management of the contents of insects. As a result of all the collections were examined, 404 species of 65 family under 3 order were identified. Therefore, the species composition of insect over each result from Woraksan National Park’s was a total of 3 orders, 72 families, 609 species including 2 Endangered species, 6 Endermic species, 10 Management of exotic species and 50 Designated species; 368 species of family Coleoptera which is the highest, 126 species of family Hemiptera and 115 species of family Lepidoptera. Site 3 had the highest indices diversity. In contrast, Site 10 indices diversity, different from the other survey points, were significantly low. In each survey period, dominant species included Nicrophorus quadripunctatus in 2007 and 2009. In 2008, dominant species was Pachygrontha antennata.

좁고 구불구불한 후방 순환계의 뇌동맥류에 대한 두 개의 유도 도관을 이용한 코일색전술

김진욱,박용석 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

양쪽의 추골 동맥이 모두 구불구불하거나, 혈관 내경이 모두 좁을 때에는 유도 도관을 추골 동맥 안에 설치할 수가 없다. 저자들은 두 개의 유도 도관을 각각의 추골 동맥 입구에 설치한 후에 기저 동맥 상위부 뇌동맥류를 코일 색전술로 치료한 증례를 보고한다. When both of the vertebral arteries are tortuous or narrow, it is impossible to place the guiding catheter in the vertebral artery(VA). We present a case of ruptured basilar top aneurysm which was treated by coil embolization placing two guiding catheters at each origins of the VAs.

긴볼레기말의 항고지혈증 효과에 관한 연구 : Triton WR-1339 주사에 의한 고지혈증 유발 생쥐의 간세포내 지방 축적 감소를 중심으로 Based on the Decreasing Effect of Lipid Accumulation in Hepatocyte of Murine with Hyperlipidemia induced by Triton WR-1339

박인식,안상현,정재만,강윤호,이해풍,서귀문,홍용기,김호현,김진택 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 긴볼레기말 추출물의 항고지혈증 효과를 조사하기 위해 ICR 생쥐에 TritonWR-1339(TX) 복강주사로 인위적인 고지혈증을 유발시킨 후 긴볼레기말 추출물(30㎎/㎏)를 복강주사하여 시간의 경과에 따른 간세포내에서의 지방 축적 변화를 조직화학적으로 관찰하였다. TX 주사후 그물구조의 세포질출 가진 간세포가 간엽 전체에서 관찰되었고, 일부 간소엽에서는 간세포 손상으로 인한 간세포판 소실이 나타났다. 또한 간세포내 지방축척도 증가하여 전체 간소엽의 간세포에서 지방의 과출현을 확인 할 수 있었고, 지방의 크기도 대조군에 비해 증가된 것으로 관찰되었다. 그러나 긴볼레기말 추출물 주사군에서는 그물구조의 세포질을 가진 간세포의 수가 TX 주사군에 비해 감소되었고, 대부분의 간소엽에서 정상적인 간세포판의 배열을 확인할 수 있었다. 간세포내의 지방 축적과 크기도 감소된 경향으로 관찰되었다. 이상의 결과로 볼 때 해조류 긴볼레기말 추출물은 고지혈증이 유발된 생쥐 간세포 내에서의 과도한 지방축적을 감소시키는 항고지혈증 효과를 하는 것으로 사료된다. Hepatic tissues of ICR mouse were intraperitoneally injeced with Colpomenia bullosa(CB) Extract after Triton WR-1339(TX) injection were observed to investigate the antihyperlipermic effect of CB extract for hyperlipidemic hepatic tissue caused by destruction of lipid metabolism. The hepatic tissues were obtained at hour-24, 48, and 72 after TX injection with CB extract treatment. And then these specimen were fixed in 10% neutral buffer solution and were cryocut. The tissue stained by H&E for general morphology and sudan black B for lipid distribution. The increase of hepatocyte having rneshlike cytoplasm were shown in all hepatic lobules after TX injection and the hepatic plates were disappeared in the region of meshlike hepatocyte aggregation, But the hepatocyte having meshlike cytoplasm were disappeared and hapatic plate were rearranged in CB extract injected mouse. The number of blue black colored lipid drop in hepatic cytoplasm of mouse injected with TX were increased and the size of lipid drop were enlarged. But the number of lipid drop in hepatic cytoplasm of mouse treated CB extract were decreased and the size of lipid drop were diminished. As results indicated that the accumulation of lipid drop caused by TX injection were mitigated by the antihyperlipidermic effect of CB extract.

Sn-P 첨가가 박육 구상 흑연주철의 미세구조와 기계적 성질에 미치는 영향

朴龍鎭,朴仁善 弘益大學校 科學技術硏究所 1996 科學技術硏究論文集 Vol.7 No.1

In this study, as the necessity of thin section and high strength of Spheroidal Graphite Cast iron increase, the effect of Oscillation and Sn-P addition on Microstructure and Mechanical properties of Spheroidal Graphite Cast iron was investigated. As a result, by decreasing casting thickness, the size of speroidal graphite grew smaller, on the contrary, the number of speroidal graphite increased in case of Oscillation and Sn-P added, also shape of graphites were K type in case Oscillation but contrarily L type in case non Oscillation. The tensile strength and hardness of Spheroidal Graphite Cast iron increased with the amount of Sn-P untill its contents reaches to 0.2%, however it decreases when the amount of P exceeds 0.2%. Therefore, as a result of thin section of casting, the mechanical properties of Spheroidal Graphite Cast iron will be improved, Oscillation and added 0.2%Sn-0.2%P will take effect on high strength of casting.

제 2형 당뇨병 환자에서 대사증후군의 유병률 및 관련요인의 특징

박철진,박거운,박일구,신지혜,김진화,배학연,김상용 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.3

Background: People with insulin resistance has a higher risk of developing cardiovascular disease. This leads to the importance in diagnosing metabolic syndrome. The incidence of metabolic syndrome has increased significantly in korea because of an increased population of obese people and also the incidence of DM. There have been many studies done on the incidence of metabolic syndrome in the general population. Therefore, the authors have designed this study to identify the incidence of metabolic syndrome in patients with type 2 DM and also the associated characteristics in that population. Methods: The study included 169 adults over the age of 20 (84 males and 85 females) admitted to the endocrinology department of Chosun University between Jan, 1st 2005 and May, 31st 2006. Out of the people diagnosed with type 2 DM people were excluded when any of the physical measurements (height, weight, blood pressure, abdominal circumference) and blood measurements (TG, HDL-cholesterol, fasting blood sugar) were insufficient and when the patient was taking any drugs other than anti-hy-pertensive agents and oral anti-diabetic agents. Obesity and the other risk factors were defined by the Asian-Pacific guideline and 2001 ATP III of NCEP, respectively. Result: The each prevalence according to the metabolic syndrome criteria of the type 2 DM patients showed low HDL cholesterolemia with the highest incidence of 57.4% followed by hypertng1yceridemia(52.0%), obesity(40.9%) and hypertension(33.0%). The each incidence of diagnostic criteria in metabolic syndrome according to sex was different, In males, hypertriglyceridemia was the highest with the incidence of 57.7% followed by low HDL-cho1estero1emia(49.5%), obesity(40.9%) and hypertension (30.0%). In women, the incidence were low HDL-cho1estero1emia(64.8%), hyperthg1yceridemia(47.6%), obesity(45.0%) and hypertension(36.0%), in a descending order. The analysis between the diagnosis of metabolic syndrome in type 2 DM patients and the criteria showed that hyperthglyceridemia (correlative value 0.575) had the highest correlation followed by low HDL-cholesterolemia (correlative value 0.446), obesity (correlative value 0.350) and hypertension (correlative value 0.410). All the correlations were statistically significant with a p-value less than 0.01. Conclusions: Type 2 DM is a important factor of metabolic syndrome based on insulin resistance. The control of blood glucose with prevention and early diagnosis of the other factors related to metabolic syndrome in type 2 DM is important for preventing cardiovascular complications.