UBE2W Interacts with FANCL and Regulates the Monoubiquitination of Fanconi Anemia Protein FANCD2

Yingying Zhang,Peitang Huang,Xiaowei Zhou,Lixia Zhao,Chao Li,Hengqi Zhu,Long Xu,Liran Shan,Xiang Liao,Zekun Guo 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.2

Fanconi anemia (FA) is a rare cancer-predisposing ge-netic disease mostly caused by improper regulation of the monoubiquitination of Fanconi anemia complementation group D2 (FANCD2). Genetic studies have indicated that ubiquitin conjugating enzyme UBE2T and HHR6 could regulate FANCD2 monoubiquitination through distinct mechanisms. However, the exact regulation mechanisms of FANCD2 monoubiquitination in response to different DNA damages remain unclear. Here we report that UBE2W, a new ubiquitin conjugating enzyme, could regulate FANCD2 monoubiquitination by mechanisms different from UBE2T or HHR6. Indeed, UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL) in vitro. UBE2W binds to FANCL, and the PHD domain is both necessary and sufficient for this interaction in mammalian cells. In addition, over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradia-tion-induced but not MMC-induced FANCD2 monoubiquiti-nation. These results indicate that UBE2W regulates FANCD2 monoubiquitination by mechanisms different from UBE2T and HRR6. It may provide an additional regulatory step in the activation of the FA pathway.

Seismic Behaviors of Steel Bar Reinforced Joints of Concrete Filled Steel Tubular Laminated Columns

Yingying Zhang,Yuan Huang,Ke Lei,Jianing Pei,Qilin Zhang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.9

This paper presents the numerical studies on seismic behaviors of steel bar reinforced joints of Concrete Filled Steel Tubular Laminated Columns (CFSTLC). First, the material constitutive relations and modeling details are introduced. Then, the failure mode, bearing capacity and energy dissipation of CFSTLC joints under cyclic loading are studied, in which the effects of concrete grade, steel grade and reinforcement arrangement are discussed. Finally, the restoring force model is proposed to describe the seismic behaviors of bar reinforced joints. Results show that the bar reinforced joints perform good seismic performance. There are three main failure modes, including flexural failure of beam, shear failure of column and mixed failure. Increasing concrete grade and reinforcement ratio of column can increase the ultimate bearing capacity and energy dissipation capacity. Using bundled longitudinal reinforcement bars in beams can slightly reduce the ultimate bearing capacity, but can improve the energy dissipation capacity of specimens with flexural failure modes. Increasing the reinforcement ratio and steel grade can decrease the degradation rate of structural stiffness. The tri-linear restoring force model can make a good prediction of hysteretic behaviors of CFSTLC joints.

Seismic behaviors of ring beams joints of steel tube-reinforced concrete column structure

Yingying Zhang,Jianing Pei,Yuan Huang,Ke Lei,Jie Song,Qilin Zhang 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.27 No.4

This paper presents the seismic behaviors and restoring force model of ring beam joints of steel tube-reinforced concrete column structure under cyclic loading. First, the main failure mode, ultimate bearing capacity, stiffness degradation and energy dissipation capacity are studied. Then, the effects of concrete grade, steel grade, reinforcement ratio and radius-to-width ratios are discussed. Finally, the restoring force model is proposed. Results show that the ring beam joints of steel tube-reinforced concrete column structure performs good seismic performances. With concrete grade increasing, the ultimate bearing capacity and energy dissipation capacity increase, while the stiffness degradation rates increases slightly. When the radius-width ratio is 2, with reinforcement ratio increasing, the ultimate bearing capacity decreases. However, when the radius-to-width ratios are 3, with reinforcement ratio increasing, the ultimate bearing capacity increases. With radius-to-width ratios increasing, the ultimate bearing capacity decreases slightly and the stiffness degradation rate increases, but the energy dissipation capacity increases slightly.

Improving the charge transfer performance of Si nanomaterial through C surface modification: A first-principles study

Yingying Yang,Xiaowan Xue,Yuan Qin,Hao Huang,Xudong Wang,Man Yao 한국물리학회 2019 Current Applied Physics Vol.19 No.7

Models of oxidized Si (111) surface under different C coverage were established to study the charge transfer ability of Si nanomaterial from strategy of C surface modification using first-principles calculation. The calculated formation energies show that structures of C surface-modified oxidized Si (111) surface are stable. The electronic properties present that the interaction between C and Si atoms is mainly contributed by the hybridization of C-2p and Si-3p states. And the interaction between C and Si atoms increases firstly and then decreases with the increasing C coverage rate. The transfer charge between C and Si reaches a maximum when C coverage rate is 0.5. We speculate that the conductivity of experimentally prepared Si nanomaterial does not monotonously enhance with the increasing C concentration, which would reach a maximum at a certain C concentration, and then decreases.

Vibration Analysis of Switched Reluctance Motor Based on Multi-objective Genetic Algorithm

Huang Xiaocun,Rao Yingying,Jing Libing 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.2

The vibration and noise caused by radial electromagnetic force hinder the development of switched reluctance motor (SRM). A structure with auxiliary slots in the stator teeth and rotor teeth of SRM is proposed in this paper. The radial force density and average torque are optimized by multi-objective genetic algorithm, and the optimal structural parameters of the motor are determined. The modal analysis and harmonic response analysis of the stator are carried out by using Workbench finite element software and compared with the conventional SRM. The results show that the radial force density is reduced by 42.19%, which verifies that opening auxiliary slots in stator teeth and rotor teeth is conducive to reducing the vibration of SRM.

Exploring the Factors Affecting Behavioral Intentions of Spectators: Case Study of 2019 FIBA Basketball World Cup

( Shihao Huang ),( Yinggang Chen ),( Xia Xu ),( Yingying Gong ),( Bin Xu ) 아시아스포츠융합과학회 2021 Asia Pacific Journal of Applied Sport Sciences Vol.2 No.3

PURPOSE In this study, The event-destination image transfer model was used to predict and test the behavioral intention of spectators watching major sports events. Based on The Event-destination image transfer model, this study added place attachment as a new variable to investigate The influencing factors of audience decision-making process. METHOD This study sampled 500 spectators of the 2019 FIBA Basketball World Cup held in Beijing, China. The 2019 FIBA Basketball World Cup was held in Beijing, Guangzhou, Nanjing, Shanghai, Wuhan, Shenzhen, Foshan and Dongguan on September 15, 2019. RESULT After spectators attend a mega-event held in a tourism destination, the event image positively affected the destination image. Destination image directly and positively affected spectators' behavioral intentions toward the destination. Tourists' overall attitude toward the destination was influenced positively by both the destination image and event image. CONCLUSION There are three significant antecedents factors for the behavioral intention of the destination, namely the image of the destination, tourists' overall attitude towards the destination and place attachment. The destination image directly and positively affects place attachment. Place attachment directly and positively affects tourists' behavioral intentions toward the destination.

Proteomic analyses on the browning of shade-dried Thompson seedless grape

Liu Fengjuan,Huang Wenshu,Feng Zuoshan,Tao Yongxia,Fan Yingying,He Weizhong,Li XiaoLi,Fang Xiaotong,Wang Cheng,Bai Yujia 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.3

China is one of the main producers in the worldwide raisin market. Most China’s raisins are produced in Xinjiang where the Thompson seedless grape ( Vitis vinifera L.cv.Thompson seedless) is the main variety of green raisin. However, the browning of Thompson seedless grape during drying has been well-acknowledged as the primary factor affecting the development of the raisin industry. Data independent acquisition (DIA)-based protein profiling was performed on fresh and shade-dried Thompson seedless grapes. As a result, 5431 proteins were identified, among which the amounts of 739 proteins in fresh grape were found to be significantly different with those in dried grape. The functional annotation based on the Blast2GO showed that the ‘organic substance metabolic process’, ‘regulation of molecular function’, ‘enzyme regulator activity’, and ‘isomerase activity’ related proteins became very active during browning. Further analyses revealed that the browning-related proteins, which with significant different amounts in fresh and in dried grapes, are primarily involved in the phenylpropanoid biosynthesis, tyrosine metabolism, phenylalanine metabolism, oxidative phosphorylation metabolism, plutathione metabolism, peroxisome pathway, and fatty acid degradation. And five random differential proteins were verified with parallel reaction monitoring (PRM). The PRM results were in agreement with the DIA data. The main browning-related proteins of Thompson seedless grape were identified in this study. Their properties were tested, and their roles in the browning mechanism were indicated. This will lay base to a better understanding on the enzymatic browning of Thompson seedless grape, and it will also provide guidance for controlling the quality of Thompson seedless grapes in industry.

UV-Light Photoelectrochemical Sensor Based on the Copper Tetraamino-Phthalocyanine-modified ITO Electrode for the Detection of Nifedipine in Drugs and Human Serum

Jinyun Peng,Wenfeng Zhuge,Yingying Huang,Cuizhong Zhang,Wei Huang 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.3

In this study, a new photoelectrochemical (PEC) sensor based on the copper tetraamino-phthalocyanine-modified ITO electrode (CuTAPc/ITO) was designed for the determination of nifedipine. The prepared CuTAPc materials were characterized using UV?Visible spectrophotometry (UVS), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). Then, the CuTAPc solution was coated on the ITO electrode surface to fabricate a simple PEC sensing platform. Then, the photoelectrochemical behavior of nifedipine at the CuTAPc/ITO electrode was investigated by chronoamperometry under ultraviolet light irradiation. The obtained results indicated that the modified electrode showed a higher response signal than the blank electrode. The photocurrent of CuTAPc/ITO increased with the increasing concentration of nifedipine. The peak photocurrents were linearly dependent on the nifedipine concentration in the range of 0.25?18.0 ?mol/L, thereby providing the detection limit of 0.15 ?mol/L (S/N = 3). Due to the good responses for the determination of nifedipine, this sensor can be used as an alternative analysis tool for the detection of nifedipine in various real samples with high sensitivity, low detection limit, and wide linearity range.

Simultaneous desulfurization and denitrogenation of model fuels by polyethylene glycol-modified resorcinol/formaldehyde resin-derived carbon spheres

Jie Wen,Dongdong Zhao,Yingying Lu,Jing Huang,Yanping Li,Hui Zhang,Airong Li 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.7

A series of resorcinol/formaldehyde (RF) resin-derived carbon spheres modified by polyethylene glycol (PEG) were prepared. The effects of PEG addition time during hydrothermal treatment and molecular weight on the pore structure, surface acidic groups, particle size, and adsorption performances of the obtained spherical activated carbons were investigated. Two types of model fuel containing indole, quinoline, and dibenzothiophene (DBT) as target adsorbates were prepared to evaluate the adsorption performances of the spheres, which increased in the order of DBT<quinoline<indole. Hydrogen bonding may be critical in the removal of indole. Both the surface area and oxygen- containing functional groups influenced the adsorption capacity, with the latter significantly influenced by PEG addition time. PEG addition during the RF resin cross-linking stage sharply reduced the total concentration of acidic groups on the carbon sphere surfaces. The adsorption thermodynamics and kinetics of nitrogen and sulfur compounds were also investigated. The adsorption isotherms of all S/N species were of the Freundlich type at 25 oC. The pseudo-second-order rate equation well described the adsorption kinetics. Both external diffusion and intra-particle diffusion controlled the rate of adsorption.

Spectroscopic Determination of the Species Fractions and the Power Profiles of the Diagnostic Neutral Beam on the HT-7 Tokamak

Jia Fu,Yuejiang Shi,Yingying Li,Fudi Wang,Sheng Liu,Jian Zhang,Jun Li,Yiyyun Huang,Yuanlai Xie,Zhimin Liu,Chundong Hu,Chundong Hu,DNB Team,William Rowan,He Huang 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.5

A diagnostic neutral beam (DNB) has been installed on the HT-7 tokamak for the measurement of charge exchange recombination spectroscopy (CXRS). The H_α-light Doppler shift spectroscopy from the drifted duct is measured to determine the components of the neutral beam. The fractions of neutral beam species are investigated under a wide range of arc voltages and extraction high voltages of the beam aimed to the optimized species fractions for the CXRS applications. A magnet ring is used to improve the magnetic property of the ion source. The result shows that the full-energy component fractions increase from 19 to 25 percent with optimization of the beam operation, but with a dramatic increase of the water component. There are nine optical fiber channels observing one section of the beam simultaneously for this spectroscopy, which provides information of the power profiles of the beam. The full width at half maximum (FWHM) of the beam profile is 8 cm, as measured by using the spectroscopy.