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Ying-Cheng Chiang,Chi-An Chen,Chun-Ju Chiang,Tsui-Hsia Hsu,Ming-Chieh Lin,San-Lin You,Wen-Fang Cheng,Mei-Shu Lai 대한부인종양학회 2013 Journal of Gynecologic Oncology Vol.24 No.4
Objective: To investigate the changes of incidence and prognosis of epithelial ovarian cancer in thirty years in Taiwan. Methods: The databases of women with epithelial ovarian cancer during the period from 1979 to 2008 were retrieved from the National Cancer Registration System of Taiwan. The incidence and prognosis of these patients were analyzed. Results: Totally 9,491 patients were included in the study. The age-adjusted incidences of epithelial ovarian cancer were 1.01, 1.37, 2.37, 3.24, 4.18, and 6.33 per 100,000 person-years, respectively, in every 5-year period from 1979 to 2008. The age-specific incidence rates increased especially in serous, endometrioid and clear cell carcinoma, and the age of diagnosis decreased from sixty to fifty years old in the three decades. Patients with mucinous, endometrioid, or clear cell carcinoma had better long-term survival than patients with serous carcinoma (log rank test, p<0.001). Patients with undifferentiated carcinoma or carcinosarcoma had poorer survival than those with serous carcinoma (log rank test, p<0.001). The mortality risk of age at diagnosis of 30-39 was significantly higher than that of age of 70 years or more (test for trend, p<0.001). The mortality risk decreased from the period of 1996-1999 (hazard ratio [HR], 0.90; p=0.054) to the period after 2000 (HR, 0.74; p<0.001) as compared with that from the period of 1991-1995. Conclusion: An increasing incidence and decreasing age of diagnosis in epithelial ovarian cancer patients were noted. Histological type, age of diagnosis, and treatment period were important prognostic factors for epithelial ovarian carcinoma.
Lai Ying-Chieh,Hsieh Ching-Yi,Juan Yu-Hsiang,Lu Kuan-Ying,Lee Hsien-Ju,Ng Shu-Hang,Wan Yung-Liang,Lin Gigin 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.5
Hyperpolarized (HP) carbon-13 (13C) MRI represents an innovative approach for noninvasive, real-time assessment of dynamic metabolic flux, with potential integration into routine clinical MRI. The use of [1-13C]pyruvate as a probe and its conversion to [1-13C]lactate constitute an extensively explored metabolic pathway. This review comprehensively outlines the establishment of HP 13C-MRI, covering multidisciplinary team collaboration, hardware prerequisites, probe preparation, hyperpolarization techniques, imaging acquisition, and data analysis. This article discusses the clinical applications of HP 13C-MRI across various anatomical domains, including the brain, heart, skeletal muscle, breast, liver, kidney, pancreas, and prostate. Each section highlights the specific applications and findings pertinent to these regions, emphasizing the potential versatility of HP 13C-MRI in diverse clinical contexts. This review serves as a comprehensive update, bridging technical aspects with clinical applications and offering insights into the ongoing advancements in HP 13C-MRI.
Bioconversion of Ginsenoside Rb1 to Compound K using Leuconostoc lactis DC201
Piao, Jin-Ying,Kim, Yeon-Ju,Quan, Lin-Hu,Yang, Dong-Uk,Min, Jin-Woo,Son, Seon-Heui,Kim, Sang-Mok,Yang, Deok-Chun The Plant Resources Society of Korea 2011 한국자원식물학회지 Vol.24 No.6
Ginseng (Panax ginseng) is frequently used in Asian countries as a traditional medicine. The major components of ginseng are ginsenosides. Among these, ginsenoside compound K has been reported to prevent the formation of malignancy and metastasis of cancer by blocking the formation of tumor and suppressing the invasion of cancer cells. In this study, ginsenoside $Rb_1$ was converted into compound K, via secreted ${\beta}$-glucosidase enzyme from the Leuconostoc lactis DC201 isolated, which was extracted from Kimchi. The strain DC201 was suspended and cultured in MRS broth at $37^{\circ}C$. Subsequently, the residue from the cultured broth supernatant was precipitated with EtOH and then dissolved in 20 mM sodium phosphate buffer (pH 6.0) to obtain an enzyme liquid. Meanwhile, the crude enzyme solution was mixed with ginsenoside $Rb_1$ at a ratio of 1:4 (v/v).The reaction was carried out at $30^{\circ}C$ and 190 rpm for 72 hours, and then analyzed by TLC and HPLC. The result showed that ginsenoside Rb1 was transformed into compound K after 72 hours post reaction.
Jun Tan,Ju Chu,Wenjuan Shi,Cheng Lin,Yuanxin Guo,Ying-ping Zhuang,Siliang Zhang,Tadayuki Imanaka 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6
Most of the fermentation experiment designs were limited by the low-throughput of shake flask, especially for the medium optimization. A simple high-throughput screening system was developed for the determination of pigment in Monascus purpureus fermentation samples. This downscaled system was designed to optimize medium composition combined with statistical methods. The total 29 experiments designed by the Box–Behnken were used to study the 4 most important operating variables on pigment production. The analysis revealed that the optimum concentrations of glucose, peptone, NaNO3, and KH2PO4were 51.42, 4.91, 1.00, and 1.00 g/L, respectively. A production of 69.5 U/mL was achieved in agreement with the prediction (68.9 U/mL) fermented in 24-deep-well microtiterplates. Furthermore, the fermentation medium optimized in the high-throughput system was verified in shake flasks, and the pigment production could be enhanced from 206.5 U/mL in un-optimized medium to 265.8 U/mL,giving nearly 1.30-fold increase in production.
Association of Interleukin-10 A-592C Polymorphism in Taiwanese Children with Kawasaki Disease
Kai-Chung Hsueh,Ying-Ju Lin,Jeng-Sheng Chang,Lei Wan,Yu-Hsin Tsai,Chang-Hai Tsai,Chih-Ping Chen,Fuu-Jen Tsai 대한의학회 2009 Journal of Korean medical science Vol.24 No.3
Elevated serum levels of interleukin-10 (IL-10) have been reported in patients with Kawasaki disease (KD). IL-10 reduces the inflammatory actions of macrophages and T cells and it may play a significant role in the regulation of inflammatory vascular damage associated with systemic vasculitis. The aim of this study was to examine whether -592 IL-10 promoter polymorphism is a susceptibility or severity marker of KD in Chinese patients in Taiwan. The study included 105 KD patients and 100 normal controls. Genotype and allelic frequencies for the IL-10 gene polymorphism in both groups were compared. There were no significant between-group differences in the genotype distribution of IL-10 A-592C gene polymorphism (P=0.08). However, the frequency of the -592*A allele was significantly increased in the patients with KD compared with controls (71.9% vs. 61.0%, P=0.019). The odds ratio for developing KD in individuals with IL-10 -592*A allele was 1.64 (95% confidence interval, 1.06-2.52) compared to individuals with the IL-10-592*C allele. No significant difference was observed in the genotype and allelic frequencies for the IL-10 A-592C polymorphism between patients with and without coronary artery lesions. The IL-10-592*A allele may be involved in the development of KD in Taiwanese children.
Shu-Jen Chang,Su-Hua Huang,Ying-Ju Lin,Yi-Yun Tsou,Cheng-Wen Lin 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.9
Rheum palmatum, Chinese traditional herb,exhibits a great variety of anti-cancer and anti-virusesproperties. This study rates antiviral activity of R. palmatumextracts and its components against Japanese encephalitisvirus (JEV) in vitro. Methanol extract of R. palmatumcontained higher levels of aloe emodin, chrysophanol, rhein,emodin and physcion than water extract. Methanol extract(IC50 = 15.04 lg/ml) exhibited more potent inhibitoryeffects on JEV plaque reduction than water extract(IC50 = 51.41 lg/ml). Meanwhile, IC50 values determinedby plaque reduction assay were 15.82 lg/ml for chrysophanoland 17.39 lg/ml for aloe-emodin, respectively. Virucidalactivity of agents correlated with anti-JEV activity,while virucidal IC50 values were 7.58 lg/ml for methanolextract, 17.36 lg/ml for water extract, 0.75 lg/ml forchrysophanol and 0.46 lg/ml for aloe-emodin, respectively. In addition, 10 lg/ml of extract, chrysophanol or aloeemodin caused 90 % inhibition of JEV yields in cells andsignificantly activated gamma activated sequence-drivenpromoters. Hence, methanol extract of R. palmatum andchrysophanol with high therapeutic index might be usefulfor development of antiviral agents against JEV.
Chang, Shu-Jen,Huang, Su-Hua,Lin, Ying-Ju,Tsou, Yi-Yun,Lin, Cheng-Wen 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.9
Rheum palmatum, Chinese traditional herb, exhibits a great variety of anti-cancer and anti-viruses properties. This study rates antiviral activity of R. palmatum extracts and its components against Japanese encephalitis virus (JEV) in vitro. Methanol extract of R. palmatum contained higher levels of aloe emodin, chrysophanol, rhein, emodin and physcion than water extract. Methanol extract ($IC_{50}=15.04{\mu}g/ml$) exhibited more potent inhibitory effects on JEV plaque reduction than water extract ($IC_{50}=51.41{\mu}g/ml$). Meanwhile, $IC_{50}$ values determined by plaque reduction assay were $15.82{\mu}g/ml$ for chrysophanol and $17.39{\mu}g/ml$ for aloe-emodin, respectively. Virucidal activity of agents correlated with anti-JEV activity, while virucidal $IC_{50}$ values were $7.58{\mu}g/ml$ for methanol extract, $17.36{\mu}g/ml$ for water extract, $0.75{\mu}g/ml$ for chrysophanol and $0.46{\mu}g/ml$ for aloe-emodin, respectively. In addition, $10{\mu}g/ml$ of extract, chrysophanol or aloe emodin caused 90 % inhibition of JEV yields in cells and significantly activated gamma activated sequence-driven promoters. Hence, methanol extract of R. palmatum and chrysophanol with high therapeutic index might be useful for development of antiviral agents against JEV.