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      • KCI등재

        Discrete-time Sliding Mode Control with Adaptive Reaching Law via Implicit Euler Method

        Cong Wang,Hongwei Xia,Yanmin Wang,Shunqing Ren 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.1

        In this paper, a novel adaptive reaching law-based discrete-time sliding mode control scheme is designed and analyzed for nonlinear disturbed systems. The higher-order sliding mode surface is used to obtain the homogeneous asymptotic accuracy of system states. The implicit Euler method is applied to the design of discrete reaching law for the first time, further to completely eliminate numerical chattering. Meanwhile, with the help of a highorder disturbance compensator, the widths of the quasi sliding mode band and attractiveness region are reduced to an adjustable order. The designed adaptive gain functions in the reaching law are able to decrease the reaching time without affecting the accuracy of sliding mode, and the finite number of steps for any initial state to enter the quasi sliding mode band is acquired. Simulation results on magnetic suspension systems are presented to validate the developed method.

      • KCI등재

        Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method

        Mudalal Mahmoud,Wang Zhanqi,Mustafa Shockry,Liu Yiping,Wang Yao,Yu Jize,Wang Shengnan,Sun Xiaolin,Zhou Yanmin 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5

        Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields. Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields.

      • KCI등재

        Fabrication of a water-retaining, slow-release fertilizer based on nanocomposite double-network hydrogels via ion-crosslinking and free radical polymerization

        Yanmin Shen,He Wang,Zijun Liu,Weikun Li,Yuhua Liu,Jingjing Li,Hong-Liang Wei,Huayun Han 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.93 No.-

        A new type of water-retaining, slow-release fertilizer (WSF) based on double-network hydrogels wasfabricated via the ion-crosslinking of sodium carboxymethyl cellulose and the free radical polymeriza-tion of polymerizable β-cyclodextrin (MAH-CD), polyethylene glycol dimethacrylate (PEGDA), acrylamide(AM), and acrylic acid (AA) with urea-loaded halloysite as an additive. The effects of the AM to AAmonomer ratio, the halloysite content, the AlCl3 content and the MAH-CD content on the swelling ratiowere studied. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), andthermogravimetric analysis (TGA) were applied to characterize the structure and properties of the WSF. The swelling behavior and water retention capacity of the fertilizer were investigated using a classicgravimetric method. The experimental results indicated that the presence of halloysite nanotubes clearlyadjusted the swelling and release properties of the WSF. Kinetic modeling indicated that the swellingmechanism and slow release behavior were consistent with a Fickian diffusion mechanism. Form theconsiderations of its properties and raw materials, the fertilizer developed here has a good prospect ofapplication and extension.

      • KCI등재

        Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells

        Jingjing Wang,Qi Su,Qing Wu,Kun Chen,Asmat Ullah,Mohsin Ahmad Ghauri,Yanmin Zhang 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.11

        Hepatocellular carcinoma (HCC) is one of themost common tumor types globally. Despite the progressmade in surgical procedures and therapeutic options, HCCremains a considerable cause of cancer-related mortality. In this study, we investigated the antitumor eff ects of sanguinarine(Sang) on HCC and its potential mechanisms. Our fi ndings showed that Sang impairs the acidic environmentof lysosomes by inhibiting cathepsin D maturation. Inaddition, Sang inhibited the formation of autolysosomes inRFP-GFP-LC3 transfected cells, subsequently suppressinglate mitophagy. Sang also induced reactive oxygen species(ROS)-dependent autophagy and apoptosis in HCC cells,which was signifi cantly attenuated following treatment witha ROS scavenger. Further investigation using autophagyinhibitors revealed that sanguinarine-induced mitochondrialdysfunction and mitophagy led to mitochondrial apoptosisin HCC cells. Immunohistochemical staining of sanguinarine-treated xenograft samples revealed that it initiated andblocked autophagy. In summary, our fi ndings suggest thatin HCC cells, Sang impairs lysosomal function and inducesROS-dependent mitophagy and apoptosis.

      • KCI등재

        A Note on the Fracture Behavior of Concrete Beams within Water Pressure under Different Loading Rates

        Yang Wang,Shaowei Hu,Jun Lu,Yanmin Qiao 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.9

        This study examined the experiment of the water fracture interactions in the concrete. In order to create water pressure in the crack, a sealing device consisting of a mechanical clamp device and a waterproof membrane was proposed. Firstly, eighteen three-point bending tests were carried out to verify the proposed sealing device under three different loading rates. Subsequently, twelve hydraulic tests were performed under different loading rates. The results showed that the sealing device had minimal influence on the fracture behavior of concrete. Besides, with the increase of internal water pressure, the carrying capacity, fracture parameters and crack opening rate of concrete beam were evidently decreased. Meanwhile, the decrease of the carrying capacity, fracture parameters, and crack opening rate induced by internal water pressure decreased with the increase of loading rate. In addition, a significant hydraulic fracturing behavior caused by water pressure was observed in the quasi-static state.

      • KCI등재

        Preparation and thermodynamic equilibrium analysis of neodymium disilicate via sol-precipitation and sintering

        Shanjun Ke,Yanmin Wang,Zhidong Pan,He-Ping Zeng 한양대학교 세라믹연구소 2020 Journal of Ceramic Processing Research Vol.21 No.3

        Neodymium disilicate powders were synthesized via a sol-precipitation method with different precipitants (i.e., ammonia,ammonium bicarbonate and urea) and subsequent sintering. The samples were characterized by X-ray diffraction. In addition,a theoretical relationship between neodymium ion concentrations in different precipitation equilibrium solutions was alsoinvestigated and analyzed based on thermodynamic equilibrium calculation. The results show that the precipitant type has animpact on the phase compositions of the precursors and the sintered products. In the synthesis, neodymium ions can becompletely precipitated as amorphous neodymium hydroxide with ammonia as a precipitate at pH 8~9. For ammoniumbicarbonate as a precipitate, the precursor type is related to both carbon concentration and pH value. The excessive hydroxideforms neodymium hydroxycarbonate in a heated urea solution. The possible formation mechanism of silicon sol in the presenceof different precipitants was discussed.

      • Sinapic Acid(SA) attnuated the DSS-induced inestinal fibrosis in C57/6BL mice by modulated the NLRP3 inflammasome activation and autophagy pathway

        Wanying Li,Yanmin Su,Zixian Wang,Zhewen Dong,Haokun Chen,Shumei Zhong,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

        To observe the effect of SA on DSS-induced intestinal fibrosis in mice. The mice were randomly divided into normal, DSS and SA treated DSS mice group. Changes in body weight(BW), blood stool and diarrhea were observed. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were determined by ELISA kits. The protein levels of NLRP3, caspase-1, ASC, IL-1 β, α-SMA, CollagenI, Beclin1, LC3II/I and p62 were detected by Western Blotting assay. Compared with the normal group, SA significantly inhibited the loss of BW and DAI score in colitis mice (P<0.05). H&E and Masson staining assay suggested the SA reduced epithelial cell damage, crypt structure loss, inflammatory cell infiltration, and the fibrogenesis in colon of colitis mice. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were decreased by SA(P<0.05). SA was able to reduce the protein levels of NLRP3, Caspase-1, ASC, IL-1β, α-SMA, Collagen-I, p62, and increased the expressions of Beclin1 and LC3II/I in colitis mice. The study demonstrated that SA can modulate the activity of NLRP3 inflammasome and autophagy pathway to improve DSS induced intaestinal fibrosis in mice.

      • KCI등재

        Experimental Study on Mechanical and Durability Properties of Glass and Polypropylene Fiber Reinforced Concrete

        Jinliang Liu,Yanmin Jia,Jun Wang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.9

        The aim of the study is to validate the effect of glass fiber and polypropylene fiber on improving the mechanicaland durability properties of concrete. In this regard, glass fiber, polypropylene fiber and hybrid fiber were added to concrete,respectively. This paper conducted the compressive and bending flexural tests to confirm that the fiber enhances themechanical properties of concrete. In order to evaluate the durability of fiber reinforced concrete, the rapid chloride migrationtest and rapid chloride penetration test were carried out. The comparisons of experimental results illustrate that the hybridfiber reinforced concrete has the most significant effect on the concrete properties improvements. Moreover, comparing withthe glass fiber reinforced concrete, the polypropylene fiber reinforced concrete plays a better performance on mechanical anddurability properties.

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