A Study on the correlation between the Freshman"s diet patterns and their physical fitness in a Western medical university, China

Haokun Chen,Shumei Zhong,Liuling Wu,Yuanfeng Sha,Qiang Qin,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Understand the dietary conditions of freshmen in a medical university, and explore the correlation between their dietary patterns and physical fitness levels. A dietary frequency survey was carried out among 2125 freshmen to evaluate their nutrient intake. Factor analysis was performed to extract common factors and determine dietary patterns. According to the "National Standards for Students" Physical health", the freshmen were tested for physical fitness, and the correlation analysis was conducted between the results of physical fitness score and dietary patterns. Four dietary patterns were identified by factor analysis, which were healthy dietary pattern (HDP), high fat and high sugar pattern (HFP/HSP), preserved food pattern and trace element pattern (TEP). Spearman rank correlation analysis showed the HDP and TEP were significantly positively correlated with the fitness test scores (r=0.064,0.062, p< 0.01), while the HFP and HSP was significantly negatively correlated (r=-0.057,p<0.01). Our results suggest that a healthy diet was associated with improved physical fitness performance, while a high intake of fatty and sweetened foods was associated with the opposite.

Process of pore pressure diffusion in saturated clay soil and impact of adsorbed water

LianSheng Tang,HaoKun Chen,Jing Song 한국지질과학협의회 2016 Geosciences Journal Vol.20 No.5

Water molecules are adsorbed onto the surface and interlayer of clay minerals and form a film, which causes that pore pressure diffusion in clayey soils differs from that in granular material. In this paper, adsorbed water is classified into strongly adsorbed water and loosely adsorbed water, which provide a better understanding of the physical mechanisms of the adsorbed water. To investigate the impact of adsorbed water on pore pressure diffusion, a series laboratory tests were conducted, including the uplift pressure test, pore pressure diffusion test and adsorbed water content test. The experimental results implies that strongly adsorbed water can neither flow nor participate in pressure diffusion, but loosely adsorbed water shears at pressure gradients and participates in laminar flow. Therefore, as long as the dense clayey soil contains loosely adsorbed water, the pore pressure change at a local site can diffuse throughout the material. A concept called “loosely adsorbed water index (Ilo) is defined to characterize both the content and physical properties of loosely adsorbed water: (1) when Ilo ≥ 1, the peripheral water molecules of loosely adsorbed water layer essentially behave the same as free water; (2) as Ilo → 0, the physical properties of adsorbed water may evolve from a gel-like state to a solid-like state (strongly adsorbed water). According to the density of clay and the content of adsorbed water, the process of pore pressure diffusion can be divided into three modes: flow channels of free fluid, deformations of the adsorbed water layer and shear motions of the peripheral-adsorbed water molecules. Pore pressure response to additional water pressure strongly depends on the permeability, seepage path and boundary conditions. The time-lag effect of pore pressure diffusion should be considered in applications of effective stress in engineering.

Impacts of organic content and pH on consolidation of clayey dredger fill by vacuum preloading method

LianSheng Tang,Jing Song,HaoKun Chen,Ya Wang,JinLong Yin,JinLong Ye 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.5

The vacuum preloading method combining prefabricated vertical drains (PVDs) and surcharge preloading from vacuum pressure is widely used in the improvement of dredger fill foundation. However, a clogging effect arises within the soils around the PVDs during the vacuum preloading, which significantly affects the soil consolidation. Previous studies indicated that the organic matter and soil pH circumstance influence the soil-water interaction and soil microstructure. In order to investigate the influences of organic matter and soil pH on the consolidation of clayey dredger fill, a series of small scale vacuum preloading tests was conducted on soils with different organic contents and soil pH values. Subsequently, Scanning Electron Microscopy observation test and vane shear test were carried out to evaluate the effect of vacuum preloading consolidation. The results showed that a low organic content or a high soil pH is beneficial for soil consolidation by vacuum preloading method, resulting in a more compact microstructure and a greater vane strength. There are two actions on the organic matter during the vacuum preloading consolidation, namely, material migration and organic matter dissolution. The organic matter dissolution effect is predominate at soil pH > 9.1, so that the consolidation effect is improved. However, the material migration effect is predominate at pH = 8.1, clay particles and organic matter can be transported with the water flow and redeposit near the drain pipes, resulting in a clogging effect. Moreover, a higher pH can induce the change of microstructure from flocculated association to deflocculated and dispersed association of clay particles, which is positive to soil consolidation. The results in this paper may offer helpful references for engineering practice.

Effects of Armillaria mellea polysaccharides (AMP) on letrozole-induced polycystic ovary syndrome (PCOS) in rats

Shumei Zhong,Wanying Li,Zixian Wang,Haokun Chen,Yaqi Wu,Chaojie Chong,Zhewen Dong,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To investigate the effects of AMP on letrozole-induced PCOS rats. All SD rats were randomly divided into normal, PCOS, metformin group (Met, 265 mg/kg) and AMP group (1 g/kg) and weighed every three days. After 21 days of continuous administration, oral glucose tolerance test was performed, and the blood glucose(BP) level was measured. After execution, calculate the relative weight of bilateral ovaries. Serum levels of FSH, LH, E2, T and FINS were detected by ELISA assay. Serum levels of TC, TG, HDL-C and LDL-C were detected by the kit and calculated the LH/FSH ratio and HOMA-IR level. Compared with the normal group, body weight and relative weight of ovaries, BP level and serum levels of T, LH, LH/FSH ratio, FINS, HOMA-IR, TC, TG, LDL-C, and E2, FSH, HDL-C in model group increased. Compared with the PCOS group, body weight in the Met group and AMP group was lower, BP level and serum levels of T, LH, FINS, HOMA-IR and LDL-C in the Met group and AMP group were significantly lower and the level of HDL-C was higher. Armillaria mellea polysaccharides can improve hormone disturbance, glucose metabolism and abnormal lipid level in letrozole-induced polycystic ovary syndrome model rats.

Effect of Antrodia camphorata polysaccharides on letrozole-induced polycystic ovary syndrome (PCOS) model rats

Zhiqiang Liu,Chaojie Chong,Zhewen Dong,Yaqi Wu,Wanying Li,Haokun Chen,Shumei Zhong,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To investigate the effect of ACP on letrozole-induced PCOS rats. All rats were divided into normal groups, PCOS group, metformin group and ACP group. The body weight (BW) was recorded every three days, and the final body weight as well as ovarian weight were recorded. Serum levels of TC, TG, HDL-C, LDL-C and E2, T, FSH and LH were measured by ELISA assay, and the LH/FSH ratio was calculated. The results showed that ACP decreased the BW and the ratio of ovarian to BW. Compared with the normal group, the serum levels of T, LH, LH/FSH, TG, TC, LDL-C increased, and the levels of FSH, E2, HDL-C decreased in PCOS group. ACP effectively improves the serum levels of FSH, LH/FSH, TG, TC, LDL-C and other related factors. The results of H&E staining of ovarian tissues showed that the ACP group showed different degrees of improvement in the reduced number of corpus luteum, thinning of granulosa cell layer, follicular capsular dilatation and follicular atresia. ACP not only reduces BW and the ratio of ovarian to BW, but also it can elevate the level of FSH and reduce LH/FSH, TG, TC and HDL-C levels. Furthermore, ACP can protect ovarian tissue to some extent.

Sinapic Acid(SA) attnuated the DSS-induced inestinal fibrosis in C57/6BL mice by modulated the NLRP3 inflammasome activation and autophagy pathway

Wanying Li,Yanmin Su,Zixian Wang,Zhewen Dong,Haokun Chen,Shumei Zhong,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To observe the effect of SA on DSS-induced intestinal fibrosis in mice. The mice were randomly divided into normal, DSS and SA treated DSS mice group. Changes in body weight(BW), blood stool and diarrhea were observed. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were determined by ELISA kits. The protein levels of NLRP3, caspase-1, ASC, IL-1 β, α-SMA, CollagenI, Beclin1, LC3II/I and p62 were detected by Western Blotting assay. Compared with the normal group, SA significantly inhibited the loss of BW and DAI score in colitis mice (P<0.05). H&E and Masson staining assay suggested the SA reduced epithelial cell damage, crypt structure loss, inflammatory cell infiltration, and the fibrogenesis in colon of colitis mice. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were decreased by SA(P<0.05). SA was able to reduce the protein levels of NLRP3, Caspase-1, ASC, IL-1β, α-SMA, Collagen-I, p62, and increased the expressions of Beclin1 and LC3II/I in colitis mice. The study demonstrated that SA can modulate the activity of NLRP3 inflammasome and autophagy pathway to improve DSS induced intaestinal fibrosis in mice.

The effect of cyanidin-3,5-O-diglucoside on the improvement of dextran sodium sulfate-induced intestinal fibrosis in mice with colitis

Zixian Wang,Wanying Li,Zhewen Dong,Chaojie Chong,Yaqi Wu,Shumei Zhong,Haokun Chen,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To observe the effect of cyanidin-3,5-O-diglucoside(C3G) on intestinal fibrosis in a model of dextran sulphate sodium (DSS)-induced mice with colitis. Colitis was induced in C57BL/6 mice by addition of DSS to drinking water. Clinical (body weight, colon length and colon weight) and histologic changes were evaluated. In addition, colon levels of interleukin-1β, IL-6, IL-18, IL-17A, tumor necrosis factor(TNF)-α were detected by enzyme-linked immunosorbent assay. Relative to the DSS group, the mice in the C3G treatment group increased in body weight, colon length, and the ratio of colon weight to length was significantly lower (P<0.05). The DAI index of mice in the intervention group was lower than that in the colitis model group (P<0.05). Histological observation also found that the intervention of C3G is able to reduce the infiltration of inflammatory cells in colon tissue induced by DSS. The results of Masson staining suggest that the intervention of C3G also reduces the occurrence of intestinal fibrosis in mice with colitis. Our results suggested that C3G is an activity to improve inflammation and intestinal fibrosis in DSS colitis mice.

Effect of Phellinus linteus polysaccharides on improving polycystic ovary syndrome (PCOS) induced by letrozole in SD rats

Yaqi Wu,Chaojie Chong,Zhewen Dong,Zixian Wang,Haokun Chen,Shumei Zhong,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To explore the ameliorative effect of Phellinus linteus polysaccharide(PLP) on letrozole-induced PCOS rats. A total of 32 female SD rats were randomly divided into normal group, PCOS group, metformin group and PLP group, and weighed every three days. The estrous cycle of rats was observed by vaginal smear ten days before sacrifice. The estrous cycle of PCOS rats was disordered, the ovaries showed polycystic changes and the body weight and the relative weight of the ovaries increased (P<0.05). The estrous cycle, polycystic ovary, body weight and relative weight of the ovaries in PLP group and Metformin group recovered significantly (P<0.05). The serum levels of TC, TG, LDL-C, HDL-C, FINS, T, LH, E2 and FSH were detected by ELISA kits. After treatment, the serum levels of TC, TG, LDL-C, FINS, T, LH and LH/FSH in the PLP group and Metformin group were lower than PCOS group (P<0.05). In addition, the serum levels of E2, FSH and HDL-C were increased (P<0.05) in the PLP group and Metformin group. The results showed that Phellinus linteus polysaccharides can ameliorate glucose and lipid metabolism, polycystic ovary and regulate the levels of sex hormones in PCOS rats.

Effect of Grifola frondosa polysaccharides (GFPS) on the improvement of clinical symptoms in letrozole-induced polycystic ovary syndrome (PCOS) rats

Chaojie Chong,Yaqi Wu,Zhewen Dong,Zixian Wang,Shumei Zhong,Haokun Chen,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study was to investigate the ameliorative effect of GFPS on clinical symptoms in letrozole(1 mg/kg)-induced PCOS rats. The female SD rats were classified into 4 groups as follows: the normal group (gavaged with saline), the PCOS group(ingested letrozole daily for 21 days). Normal group and metformin group gavaged with letrozole followed by metformin (265 mg/kg) and GFPS(600 mg/kg), respectively. All rats were weighed every 3 days until the end of the experiment. The ovaries, perirenal, abdomen and periuterine fat were removed and the relative weights (g/bw×100) were calculated. Serum levels of FSH, LH, E2, T, TG, TC, LDL-C were measured by ELISA and calculated the LH/FSH ratio. GFPS reduced follicle number and increases granulosa cell layer thickness and luteal number. GFPS also reduced the increase of body weight, ovarian and visceral fat weight in PCOS rats (P<0.01). In addition, GFPS reduced the serum levels of T, LH/FSH, TC, TG, LDL-C and LH, and elevated the serum levels of E2 and FSH in PCOS rats. In conclusion, GFPS can improve follicular development and restore body weight, ovarian weight, serum hormone levels and lipid profile in PCOS rats.

Protective effect of centaureidin-3,5-O-biglucoside (C3G) on 5-fluorouracil(5-FU)-induced mucositis in BALB/c mice

Junyang Liu,Zixian Wang,Wanying Li,Zhewen Dong,Chaojie Chong,Yaqi Wu,Shumei Zhong,Haokun Chen,Zhiqiang Liu,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To explore the effect of C3G on intestinal mucosal injury in 5-FU induced BALB/c mice. Mice were randomly divided into normal, model and C3G groups. After the last administration, mice were anesthetized, and calculated the thymus and spleen indexes. The ileum was used to stain with H&E observation. Serum levels of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA kits. Compared with the normal group, the model group had diarrhea, body weight and thymus and spleen index decreased significantly, intestinal villus epithelial cells fell off, crypt structure was destroyed, and inflammatory cell infiltration was observed. The body weight, thymus and spleen indexes of the treatment group and the prevention group decreased less, and the levels of TNF-α, IL-1β and IL-6 were significantly decreased, and the level of IL-10 was significantly increased relative to the model group. The intestinal villi structure was relatively complete, and a small amount of crypt structure was destroyed, with a small amount of inflammatory cell infiltration, which reduced the mucosal damage. The administration of C3G could protect intestinal mucosa from 5-FU-induced injury.