An Enhancer Mutant of Arabidopsis salt overly sensitive 3 Mediates both Ion Homeostasis and the Oxidative Stress Response

Zhu, Jianhua,Fu, Xinmiao,Koo, Yoon Duck,Zhu, Jian-Kang,Jenney Jr., Francis E.,Adams, Michael W. W.,Zhu, Yanmei,Shi, Huazhong,Yun, Dae-Jin,Hasegawa, Paul M.,Bressan, Ray A. American Society for Microbiology 2007 Molecular and cellular biology Vol.27 No.14

<B>ABSTRACT</B><P>The myristoylated calcium sensor SOS3 and its interacting protein kinase, SOS2, play critical regulatory roles in salt tolerance. Mutations in either of these proteins render <I>Arabidopsis thaliana</I> plants hypersensitive to salt stress. We report here the isolation and characterization of a mutant called <I>enh1-1</I> that enhances the salt sensitivity of <I>sos3-1</I> and also causes increased salt sensitivity by itself. <I>ENH1</I> encodes a chloroplast-localized protein with a PDZ domain at the N-terminal region and a rubredoxin domain in the C-terminal part. Rubredoxins are known to be involved in the reduction of superoxide in some anaerobic bacteria. The <I>enh1-1</I> mutation causes enhanced accumulation of reactive oxygen species (ROS), particularly under salt stress. ROS also accumulate to higher levels in <I>sos2-1</I> but not in <I>sos3-1</I> mutants. The <I>enh1-1</I> mutation does not enhance <I>sos2-1</I> phenotypes. Also, <I>enh1-1</I> and <I>sos2-1</I> mutants, but not <I>sos3-1</I> mutants, show increased sensitivity to oxidative stress. These results indicate that ENH1 functions in the detoxification of reactive oxygen species resulting from salt stress by participating in a new salt tolerance pathway that may involve SOS2 but not SOS3.</P>

Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance.

Zhu, Jianhua,Jeong, Jae Cheol,Zhu, Yanmei,Sokolchik, Irina,Miyazaki, Saori,Zhu, Jian-Kang,Hasegawa, Paul M,Bohnert, Hans J,Shi, Huazhong,Yun, Dae-Jin,Bressan, Ray A National Academy of Sciences 2008 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.105 No.12

<P>Histone modification in chromatin is one of the key control points in gene regulation in eukaryotic cells. Protein complexes composed of histone acetyltransferase or deacetylase, WD40 repeat protein, and many other components have been implicated in this process. Here, we report the identification and functional characterization of HOS15, a WD40-repeat protein crucial for repression of genes associated with abiotic stress tolerance through histone deacetylation in Arabidopsis. HOS15 shares high sequence similarity with human transducin-beta like protein (TBL), a component of a repressor protein complex involved in histone deacetylation. Mutation of the HOS15 gene renders mutant plants hypersensitive to freezing temperatures. HOS15 is localized in the nucleus and specifically interacts with histone H4. The level of acetylated histone H4 is higher in the hos15 mutant than in WT plants. Moreover, the stress inducible RD29A promoter is hyperinduced and associated with a substantially higher level of acetylated histone H4 in the hos15 mutant under cold stress conditions. Our results suggest a critical role for gene activation/repression by histone acetylation/deacetylation in plant acclimation and tolerance to cold stress.</P>

A Z-scheme CuO–ZnO–ZnS–CuS quaternary nanocomposite for solar-light-driven photocatalytic performance

Zhu Wenli,Yang Qiaoling,Du Juan,Yin Pinpin,Yi Jun,Liu Yanmei,Wu Xuemei,Zhang Zhongyi 한국물리학회 2022 Current Applied Physics Vol.39 No.-

A quaternary CuO–CuS–ZnO–ZnS nanocomposite was successfully synthesized via a facile microwave irradiation based on the preprepared ZnS and CuO nanoparticles. CuO–CuS–ZnO–ZnS nanocomposite was a porous photocatalyst, providing excellent adsorption performance. It was sensitive to both ultraviolet and visible light, moreover, the photoelectrochemical measurements confirmed that there was a high separation rate and low recombination rate of photo-generated charge carriers in the nanocomposite, endowing excellent photocatalytic activity in the sunlight. Under the simulated solar light irradiation, the removal efficiency of rhodamine B (RhB) pollutant (30 mg/L) over CuO–CuS–ZnO–ZnS nanocomposite was 33.98 and 2.90 times of pristine CuO and ZnS, respectively. The outstandingt photocatalytic performance was attributed to Z-scheme charge transfer path.

Evaluation and Comparison of Predictive Value of Tumor Regression Grades according to Mandard and Becker in Locally Advanced Gastric Adenocarcinoma

Yilin Tong,Yanmei Zhu,Yan Zhao,Zexing Shan,Dong Liu,Jianjun Zhang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1

Purpose Tumor regression grade (TRG) has been widely used in gastrointestinal carcinoma to assess pathological responses to neoadjuvant chemotherapy (NCT). There are various standards without a consensus, and it is still unclear which kind of system has better predictive value. This study aims to investigate and compare the predictive ability of the Mandard and Becker TRGs in patients with locally advanced gastric cancer. Materials and Methods A total of 290 patients with locally advanced gastric adenocarcinoma who underwent NCT and curative surgery were studied. Survival analysis for overall survival (OS) and disease-free survival (DFS) were based on the Kaplan-Meier method and Cox proportional hazards method. Predictive values of TRGs and models were assessed by time-dependent receiver operating characteristic (ROC) curve, the area under the ROC curve (AUC), nomogram, and calibration curve. Results In multivariable analysis, the Mandard TRG was associated with OS (hazard ratio [HR], 1.806; p=0.026) and DFS (HR, 1.792; p=0.017). The Becker TRG was also related to OS (HR, 1.880; p=0.014) and DFS (HR, 1.919; p=0.006). The Mandard and Becker TRG AUCs for 5-year survival were 0.72 and 0.71, respectively. The whole models showed an increased predictive value, with AUCs of 0.85 and 0.86, respectively. There was no significant difference between the two TRGs and two models. Conclusion TRG was an independent predictor for survival, and there was no significant difference between these two systems.

Optimized Ciphertext-Policy Attribute-Based Encryption with Efficient Revocation

Yang Li,Jianming Zhu,Xiuli Wang,Yanmei Chai,Shuai Shao 보안공학연구지원센터 2013 International Journal of Security and Its Applicat Vol.7 No.6

Epoxyeicosatrienoic Acid Inhibits the Apoptosis of Cerebral Microvascular Smooth Muscle Cells by Oxygen Glucose Deprivation via Targeting the JNK/c-Jun and mTOR Signaling Pathways

Qu, Youyang,Liu, Yu,Zhu, Yanmei,Chen, Li,Sun, Wei,Zhu, Yulan Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.11

As a component of the neurovascular unit, cerebral smooth muscle cells (CSMCs) are an important mediator in the development of cerebral vascular diseases such as stroke. Epoxyeicosatrienoic acids (EETs) are the products of arachidonic acid catalyzed by cytochrome P450 epoxygenase. EETs are shown to exert neuroprotective effects. In this article, the role of EET in the growth and apoptosis of CSMCs and the underlying mechanisms under oxygen glucose deprivation (OGD) conditions were addressed. The viability of CMSCs was decreased significantly in the OGD group, while different subtypes of EETs, especially 14,15-EET, could increase the viability of CSMCs under OGD conditions. RAPA (serine/threonine kinase Mammalian Target of Rapamycin), a specific mTOR inhibitor, could elevate the level of oxygen free radicals in CSMCs as well as the anti-apoptotic effects of 14,15-EET under OGD conditions. However, SP600125, a specific JNK (c-Jun N-terminal protein kinase) pathway inhibitor, could attenuate oxygen free radicals levels in CSMCs as well as the anti-apoptotic effects of 14,15-EET under OGD conditions. These results strongly suggest that EETs exert protective functions during the growth and apoptosis of CSMCs, via the JNK/c-Jun and mTOR signaling pathways in vitro. We are the first to disclose the beneficial roles and underlying mechanism of 14,15-EET in CSMC under OGD conditions.

Epoxyeicosatrienoic Acid Inhibits the Apoptosis of Cerebral Microvascular Smooth Muscle Cells by Oxygen Glucose Deprivation via Targeting the JNK/c-Jun and mTOR Signaling Pathways

Youyang Qu,Yu Liu,Yanmei Zhu,Li Chen,Wei Sun,Yulan Zhu 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.11

As a component of the neurovascular unit, cerebral smooth muscle cells (CSMCs) are an important mediator in the development of cerebral vascular diseases such as stroke. Epoxye-icosatrienoic acids (EETs) are the products of ara-chidonic acid catalyzed by cytochrome P450 epoxygenase. EETs are shown to exert neuroprotective effects. In this article, the role of EET in the growth and apoptosis of CSMCs and the underlying mechanisms under oxygen glucose deprivation (OGD) conditions were addressed. The viability of CMSCs was decreased significantly in the OGD group, while different subtypes of EETs, especially 14,15-EET, could increase the viability of CSMCs under OGD conditions. RAPA (serine/threonine kinase Mammalian Target of Rapamycin), a specific mTOR inhibitor, could elevate the level of oxygen free radicals in CSMCs as well as the anti-apoptotic effects of 14,15-EET under OGD conditions. However, SP600125, a specific JNK (c-Jun N-terminal protein kinase) pathway inhibitor, could attenuate oxygen free radicals levels in CSMCs as well as the anti-apoptotic effects of 14,15-EET under OGD conditions. These results strongly suggest that EETs exert protective functions during the growth and apoptosis of CSMCs, via the JNK/c-Jun and mTOR signaling pathways in vitro. We are the first to disclose the beneficial roles and underlying mechanism of 14,15-EET in CSMC under OGD conditions.

Research on High Precision Servo System of Actuator Based on PID Parameter Stability Domain Under Mixed Sensitivity Constraint

Zheng HaoXin,Huang MingHui,Zhan LiHua,Zhu YanMei,Liu PeiYao 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.3

In the PID controller design, three sensitivity functions are used as the design indexes of mixed sensitivity. In order to select the weighted functions of the three sensitivity indexes, a method of determining the weighted functions based on dynamic tracking performance is proposed. The method is applied to the performance test of the servo control system of steering gear. The test results show that the hybrid sensitivity is used as the design index of the servo system PID control, and the control system has strong anti-interference ability and good tracking performance.

Influence of dragon bamboo with different planting patterns on microbial community and physicochemical property of soil on sunny and shady slopes

Liu Weiyi,Wang Fang,Sun Yanmei,Yang Lei,Chen Huihai,Liu Weijie,Zhu Bin,Hui Chaomao,Wang Shiwei 한국미생물학회 2020 The journal of microbiology Vol.58 No.11

Dragon bamboo (Dendrocalamus giganteus) is a giant sympodial bamboo species widely distributed in Asia. However, it remains unclear how dragon bamboo and soil microbes interact to affect soil properties. In this study, we investigated the planting patterns (semi-natural and artificial) on different slopes (sunny and shady) to determine the effects on soil properties and microbial community. The results showed that the soil in which dragon bamboo was grown was acidic, with a pH value of ~5. Also, the soil organic matter content, nitrogen hydrolysate concentration, total nitrogen, available potassium, and total potassium of the dragon bamboo seminatural forest significantly improved, especially on the sunny slope. In contrast, the available phosphorus level was higher in the artificial bamboo forest, probably owing to the phosphate fertilizer application. The bacterial and fungal diversity and the bacterial abundance were all higher on the sunny slope of the semi-natural forest than those in the other samples. The microbial operational taxonomic units (OTUs) shared between the shady and sunny slopes accounted for 47.8–62.2%, but the core OTUs of all samples were only 24.4– 30.4% of each sample, suggesting that the slope type had a significant effect on the microbial community. Some acidophilic microbes, such as Acidobacteria groups, Streptomyces and Mortierella, became dominant in dragon bamboo forest soil. A PICRUSt analysis of the bacterial functional groups revealed that post-translational modification, cell division, and coenzyme transport and metabolism were abundant in the semi-natural forest. However, some microorganisms with strong stress resistance might be activated in the artificial forest. Taken together, these results illustrated the influence of dragon bamboo growth on soil physicochemical property and microbial community, which might help understand the growth status of dragon bamboo under different planting patterns.