The Impact of Writing Self-efficacy and Affective Factors on Writing Proficiency of Chinese EFL High School Students

Xiaoli Chen,심재우,이희철 대한언어학회 2022 언어학 Vol.30 No.3

This study aims to investigate the relationships among writing self-efficacy, affective factors, and writing proficiency of Chinese EFL high school students. A total of 187 subjects responded to a questionnaire with 43 items, which surveyed linguistic self-efficacy, composition self-regulatory efficacy, motivation, goal orientation, task value, and anxiety held by the subjects. These subjects had also contributed their essays that were scored by two raters. The correlations among the six constructs were statistically significant (p<0.01). In addition, the results of the multiple linear regression revealed that 55.2% variance in writing proficiency was explained by the linear combination of linguistic self-efficacy, composition self-regulatory efficacy, motivation, goal orientation, task value, and anxiety. Also, it was found that the group of students with extrinsic goal orientation outperformed the other group of students with extrinsic goal orientation. Finally, some implications for enhancing English writing strategies for high school students were made.

Patterns and drivers of Vibrio isolates phylogenetic diversity in the Beibu Gulf, China

Chen Xing,Du Hong,Chen Si,Li Xiaoli,Zhao Huaxian,Xu Qiangsheng,Tang Jinli,Jiang Gonglingxia,Zou Shuqi,Dong Ke,Adams Jonathan M.,Li Nan,Jiang Chengjian 한국미생물학회 2020 The journal of microbiology Vol.58 No.12

Members of the genus Vibrio are ubiquitous in aquatic environments and can be found either in a culturable or a viable but nonculturable (VBNC) state. Despite widespread concerns as to how to define the occurrence and dynamics of Vibrio populations by culture-independent approaches, further physiological research and relevant biotechnological developments will require the isolation and cultivation of the microbes from various environments. The present work provides data and perspectives on our understanding of culturable Vibrio community structure and diversity in the Beibu Gulf. Finally, we isolated 1,037 strains of Vibrio from 45 samples and identified 18 different species. Vibrio alginolyticus, V. cyclitrophicus, V. tasmaniensis, V. brasiliensis, and V. splendidus were the dominant species that had regional distribution characteristics. The correlation between the quantitative distribution and community structure of culturable Vibrio and environmental factors varied with the Vibrio species and geographical locations. Among them, salinity, nitrogen, and phosphorus were the main factors affecting the diversity of culturable Vibrio. These results help to fill a knowledge gap on Vibrio diversity and provide data for predicting and controlling pathogenic Vibrio outbreaks in the Beibu Gulf.

pH-Based immunoassay: explosive generation of hydrogen ions through an immuno-triggered nucleic acid exponential amplification reaction

Mao, Dongsheng,Chen, Tianshu,Chen, Huinan,Zhou, Mengru,Zhai, Xingwei,Chen, Guifang,Zhu, Xiaoli The Royal Society of Chemistry 2019 The Analyst Vol.144 No.13

<P>In this work, we propose a novel concept and a proof-of-concept strategy for the fabrication of a pH-based immunoassay platform with a certain degree of universality and scalability to make it adaptable for different application scenarios. The immunoreactions for the target detection are converted to pH changes through an engineered and optimized isothermal nucleic acid amplification, named exponential amplification reaction (EXPAR). Thus, a variety of well-developed methods for pH analysis, <I>e.g.</I> pH indicators, pH-strips and pH meters, can be applied for immunoassay directly. Here, we show that this proof-of-concept strategy is applicable for both macromolecular and micromolecular antigens by adopting human platelet-derived growth factor-BB (PDGF-BB) and chloramphenicol (CAP) as the model targets, respectively. The detection can be achieved using a colorimetric pH indicator after a 15 min reaction of the immuno-triggered isothermal nucleic acid amplification. In addition, compared with the traditional enzyme-linked immunosorbent assay (ELISA), the performance of our strategy, especially the detection limits, is improved to varying degrees for different targets, making the strategy a promising alternative for diverse application scenarios of immunoassay.</P>

Recent Development of Laboratory-made Solid-phase Microextraction Fibers on the Application of Food Safety Analysis

Jingbin Zeng,Jinmei Chen,Wenfeng Chen,Xiaoli Huang,Liangbi Chen,Xi Chen 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

Solid-phase microextraction (SPME) has gained widespread acceptance in sample pretreatment due to its solventfree and easy-to-operate properties. SPME fibers are considered as a key part of SPME technique, since it primarily determines the extraction performance of the method including sensitivity, selectivity, and reproducibility. Generally speaking, target analyte with different chemical property requires fiber coating that has the best affinity towards it. Due to the lack of varieties of commercial fibers available currently, considerable efforts have been recently made to develop tailor-made fibers to fulfill increasing demands of different analysis. This paper concisely classify some SPME fiber preparation approaches such as solgel technology, physical deposition, molecularly imprinted technique, and their respective application in food safety analysis.

Equivalent Transfer Function based Multi-loop PI Control for High Dimensional Multivariable Systems

Xiaoli Luan,Qiang Chen,Fei Liu 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.2

A new equivalent transfer function (ETF) parameterization algorithm to incorporate the loop interaction effect into the design of multi-loop PI controllers for high dimensional multivariable processes is presented in this paper. The design scheme consists of two stages. In the first, by exploiting the relationship between the equivalent closed-loop transfer function and the inverse of open-loop transfer function, the analytical expression of ETF is derived. In the second stage, based on the ETF, controller parameters for each loop are determined by utilizing the existing PI tuning rules and the simple internal model control method. The proposed ETF parameterization algorithm is more accurate and reasonable compared to the conventional ETF model approximation methods. Furthermore, the advantage of the multi-loop PI controller designed by the proposed ETF is more significant when applied to higher dimensional processes with complicated interaction modes. Several typical industrial process examples show the well-balanced and robust response with the minimum integral absolute error.

Preclinical investigation of artesunate as a therapeutic agent for hepatocellular carcinoma via impairment of glucosylceramidase-mediated autophagic degradation

Chen Wenjia,Ma Zhaochen,Yu Lingxiang,MAO Xia,Ma Nan,Guo Xiaodong,Yin Xiaoli,Jiang Funeng,Wang Qian,Wang Jigang,Fang Mingliang,Lin Na,Zhang Yanqiong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Artesunate (ART) has been indicated as a candidate drug for hepatocellular carcinoma (HCC). Glucosylceramidase (GBA) is required for autophagic degradation. Whether ART regulates autophagic flux by targeting GBA in HCC remains to be defined. Herein, our data demonstrated that the dramatic overexpression of GBA was significantly associated with aggressive progression and short overall survival times in HCC. Subsequent experiments revealed an association between autophagic activity and GBA expression in clinical HCC samples, tumor tissues from a rat model of inflammation-induced HCC and an orthotopic mouse model, and human HCC cell lines. Interestingly, probe labeling identified GBA as an ART target, which was further verified by both a glutathione-S-transferase pulldown assay and surface plasmon resonance analysis. The elevated protein expression of LC3B, the increased numbers of GFP-LC3B puncta and double-membrane vacuoles, and the enhanced expression of SQSTM1/p62 indicated that the degradation of autophagosomes in HCC cells was inhibited by ART treatment. Both the in vitro and in vivo data revealed that autophagosome accumulation through targeting of GBA was responsible for the anti-HCC effects of ART. In summary, this preclinical study identified GBA as one of the direct targets of ART, which may have promising potential to inhibit lysosomal autophagy for HCC therapy.

Methods of Improving the Natural Vibration Characteristics of the Through Tied-Arch Bridge and Test Verification

Chen Qiu,Xiaoli Xie,Chuangjie Yang,Xia Qin,Mulin Pang 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.1

A through tied-arch bridge with multi-point elastic constraints (MTAB) is proposed based on the truss structure concept to improve the natural vibration characteristics of the conventional through tied-arch bridge (CTAB). Rigid inclined rods are added between the main beam and arch ribs to form an integral truss structure with arch ribs as upper chords, rigid inclined rods as web members, and the main beam as a bottom chord to increase the rigidity of the structure. Through timely system transformation, arch ribs bear all dead loads and the integral truss structure bears all the live loads so that the advantages of arch ribs and web members are maximized. The pulsation test and fi nite element analysis (FEA) were carried out to verify the superiority of the natural vibration characteristics of the MTAB based on a 50 m span test bridge. Taking a 350 m span arch bridge as an example, the natural vibration characteristics and other mechanical characteristics of the MTAB were studied and compared with that of the CTAB. The results show the MTAB has better mechanical properties and economy, especially natural vibration characteristics and stiff ness.

Breast reconstruction with tissue expansion followed by serial autologous fat transfer: a report of six cases

Chen Zhang,Xiaoli Jiang,Linan Song,Chunying Ge 대한미용의학회 2018 대한미용의학회지 Vol.2 No.1

To introduce a new method of breast reconstruction using tissue expander followed by serial autologous fat transfer (AFT). From May 1, 2013, to April 30, 2016, six breasts from six female patients (age range, 35-62 years) were reconstructed using tissue expansion followed by serial AFT. Clinical data were obtained, and each step of the operative methods was recorded. All six patients presented satisfactory symmetry in the contralateral breast. Three AFTs were performed in one patient, whereas four and five AFTs were performed in one patient and four patients, respectively. The intervals between the two adjacent AFTs were 3-5 months. A 450-ml round expander was used in this series, and the volume of each AFT was 50-150 ml. Two patients underwent mastopexy on the contralateral breasts to correct severe breast ptosis. Two patients underwent nipple-areola complex reconstruction 6 months after the last AFT. Tissue expansion followed by serial AFT is a safe and effective method for breast reconstruction. It can be an option for patients who do not prefer pedicled TRAM or DIEP flap transfer or pedicled latissimus dorsi myocutaneous flap coverage with implant placement and those who cannot afford the Brava plus fat graft.

Cryptotanshinone inhibits TNF-α-induced LOX-1 expression by suppressing reactive oxygen species (ROS) formation in endothelial cells

Xiaoli Ran,Wenwen Zhao,Wenping Li,Jingshan Shi,Xiuping Chen 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.4

Cryptotanshinone (CPT) is a natural compound isolated from traditional Chinese medicine Salvia miltiorrhiza Bunge. In the present study, the regulatory effect and potential mechanisms of CPT on tumor necrosis factor alpha (TNF-α) induced lectin-like receptor for oxidized low density lipoprotein (LOX-1) were investigated. Human umbilical vein endothelial cells (HUVECs) were cultured and the effect of TNF-α on LOX-1 expression at mRNA and protein levels was determined by Real-time PCR and Western blotting respectively. The formation of intracellular ROS was determined with fluorescence probe CM-DCFH2-DA. The endothelial ox-LDL uptake was evaluated with DiI-ox-LDL. The effect of CPT on LOX-1 expression was also evaluated with SD rats. TNF-α induced LOX-1 expression in a dose- and time- dependent manner in endothelial cells. TNF-α induced ROS formation, phosphorylation of NF-κB p65 and ERK, and LOX-1 expression, which were suppressed by rotenone, DPI, NAC, and CPT. NF-κB inhibitor BAY11-7082 and ERK inhibitor PD98059 inhibited TNF-α-induced LOX-1 expression. CPT and NAC suppressed TNF-α-induced LOX-1 expression and phosphorylation of NF-κB p65 and ERK in rat aorta. These data suggested that TNF-α induced LOX-1 expression via ROS activated NF-κB/ERK pathway, which could be inhibited by CPT. This study provides new insights for the anti-atherosclerotic effect of CPT.

Crack Detection Based on Generative Adversarial Networks and Deep Learning

Gongfa Chen,Shuai Teng,Mansheng Lin,Xiaomei Yang,Xiaoli Sun 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

This paper proposes a novel crack detection method using the three-stages detection model. Deep learning technology has been a focus of attention in the field of crack detection; however, it needs big data to train the corresponding network model. More training samples and the combination of multiple deep learning algorithms help to improve the detection performance. Therefore, this paper employed a generative adversarial network (GAN) model to generate abundant virtual crack images with similar features to real images, these virtual images are used to train the CNN classifier and DeepLab_v3+ respectively, and then the real images are used to evaluate the performance of the three-stages detection method. The results show that the proposed three-stages detection method has excellent detection effect on the crack detection is better than that of the control experiment (the NI_MIoU, NI_Accuracy, NI_F-score and NI_MCC are increased by 22.1% − 55.6%, 5.2% − 9.8%, 37.4% − 40.0% and 6.2% − 11.1%, respectively)). These results demonstrate that the three-stages detection model has made a beneficial contribution to the crack detection.