인슐린이 섬유아세포의 증식 및 세포주기에 미치는 영향

심우영,김성수,허충림,윤정빈 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.12

Background:Insulin exerts an effect on cell proliferation and inhibition of apoptosis. However, the actions of insulin on cell cycle progression and signal transduction pathway are not well understood and insulin shows diverse effects on cell proliferation depending on cell types. Objective:We attempted to understand the underlying mechanism by which insulin exerts this proliferative effect on 3T3 L1 fibroblasts by various markers of cell proliferation. Method:We investigated the effect of insulin on cell proliferation by [3H]thymidine incorporation, analyzing the cell cycle stages by flow cytometric measurement of DNA content per cell, cell counting, analysing cell division as well as the signal transduction pathway of insulin by measuring of phosphatidylinositol 3-kinase(PI3-kinase) and p44/42 mitogen-activated protein kinase (p44/42 MAPK/ERK) activity. Results:The results were as follows : 1. Total accumulated [3H]thymidine incorporation increased approximately two-fold with insulin over the 0.5% serum control at 48 h incubation, and the maximal rate of DNA synthesis was observed during 8-12 h incubation and continuously declined until 48 h without a second increase in DNA synthnesis. 2. The flow cytometric analysis of cell population distribution showed that insulin increased the cell population in S phase. 3. After insulin treatment for 48 h, cell number was increased approximately 45% in comparison with 0.5% serum control. 4. The cell division analysed after staining 3T3 L1 fibroblasts with carboxyfluorescein diacetate succinimidyl ester (CFSE). Cell division occured only once in 24h after insulin treatment. 5. Insulin stimulated PI3-kinase and p44/42 MAPK/ERK activity about three- and two-folds, respectively. Conclusion:Taken together, this data indicates that insulin stimulated the transit from G0/G1 to S phase, progressed cell cycle through G2/M phase, increased the cell number and PI3-kinase, p44/42 MAPK/ERK stimulate cell proliferation. However, under our experimental conditions, insulin has a limited efficacy for late cell cycle events required for completion of miosis and cell cycle progression into the second round and the increase of the cell number by insulin was much less than the increase of the PI3-kinase and p44/42 MAPK/ERK activity. Therefore, the authors think that another pathways other than PI3-kinase or p44/42 MAPK/ERK might be involved in the effect of insulin on cell proliferation.

Vascular Endothelial Growth Factor가 시험관내 모발의 성장에 미치는 영향

심우영,허충림,백성호,윤정빈 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.1

Background : It has been suggested that the regulation of hair growth might involve complex interaction between dermal papilla cells and hair matrix cells. Dermal papilla cells secrete diffusible factors that would act an hair matrix cells. During anagen the papilla appears to have prominent capillary loop, whereas in telogen it is nonvascularized. Vascular endothelial growth factor(VEGF) was recently reported to be produced by dermal papilla cells in rats. Objectives : We performed this study in order to evaluate the effect of VEGF on human hair growth in vitro and on the proliferation of dermal papilla cells and to define the splice forms of VEGF. Methods : To detect the isoforms of VEGF, RT-PCR was performed on RNA isolated from dermal papilla cells and RT-PCR products were hybridized with VEGF-specific oligonucleotide probe located in exon 4. Isolated human hair follicles were cultured with various concentrations of VEGF165 and VEGF121. Hair follicle growth was measured by an Olympus inverted microscope with an eyepiece measuring graticule. Results : The following results were obtained from this study. 1. Southern hybridization and size calculation of RT-PCR products revealed that mRNA species corresponding to 121, 165, 189, and 206 amino-acid forms of VEGF were praduced by cultured human dermal papilla cells. 2. 10 ng/ml of rhVEGF165, 0.1 ng/ml of rhVEGF165 and 10 ng/ml of rhVEGF121 stimulated follicle elongation in vitro(p $lt; 0.05). 3. rhVEGF165 and rhVEGF121 had no effect on the numbers and thymidine incorporation of dermal papilla cells. Conclusion : These results suggest that VEGF is produced by dermal papilla cells and is able to promote hair growth in vitro. Increased hair growth by VEGF might occur other than by proliferation of dermal papilia cells.

알레르기성 혈관염을 동반한 장간막정맥혈전증 1예

심영보,임재현,한연석,이우진,이준성,백현욱,전영빈,이정환,김유선,우광훈,문정섭 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Mesenteric venous thrombosis is an rare type of intestinal ischemia associated with significant mortality and morbidity because of its delay in diagnosis. Mesenteric vasculitis associated with allergic vasculitis can also produce an acute intestinal ischemia. We recently experienced a case of mesenteric venous thrombosis associated with allergic vasculitis, successfully treated with segmental bowel resection and anticoagulant therapy, we report this case with a brief review of the literatures. A 30-year-old man was admitted to our hospital because of severe abdominal pain and hematochezia. Abdominal CT scan showed superior mesenteric venous thrombosis extending into main portal vein. There were large amounts of ascites and the wall of the jejunum was markedly thickened. On the 5th hospital day, his abdomen was tense and diffusely tender: bowel sounds were absent. Exploratory laparotomy was performed under the impression of intestinal infarction. At laparotomy thrombosis of the mesenteric vein and gangrene of the jejunum were found. A small bowel resection with an end-to-end anastomosis was done. Histopathological examination confirmed venous bowel gangrene and showed leukocytoclastic vasculitis, the hallmark of allergic vasculitis. The patient recovered fully and was discharged on anticoagulant therapy. At follow-up after 6 months, the patient was well with no gastrointestinal symptoms.

자연어 처리 기술을 이용한 쇼핑몰 리뷰 시스템 개선

심우섭(Woo-Seob Sim),문영호(Young-Ho Moon),서한빈(Han-Bin Seo),정지원(Ji-Won Jeong) 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.11

Vitiligo with Leukotrichia Following Topical Diphenylcyclopropenone Immunotherapy for Acute Diffuse and Total Alopecia

( Jong Bin Park ),( Seol Hwa Seong ),( Ji Yun Jang ),( Myeong Hyeon Yang ),( Joon Hee Kim ),( Kee Suck Suh ),( Woo Young Sim ),( Min Soo Jang ) 대한피부과학회 2019 大韓皮膚科學會誌 Vol.57 No.2

Leukotrichia is defined as depigmentation of hair within vitiligo macules and is considered indicative of the destruction of the melanocyte reservoir within the hair follicle. A 15-year-old woman with acute diffuse and total alopecia (ADTA) presented with white hair growth with vitiligo on the right half of the scalp after 6 cycles of diphenylcyclopropenone (DPCP) application for ADTA. At the start of treatment, black hair growth was observed on the untreated, left half of the scalp. Seven months later, sustained growth of white hair on the right side of scalp was observed without further treatment. A diagnosis of vitiligo with leukotrichia following DPCP immunotherapy was made. She was treated with narrowband ultraviolet B (NBUVB) phototherapy three times per week. The vitiligo lesion with leukotrichia disappeared almost completely after 82 sessions of NBUVB phototherapy over 18 months. Herein, we report a 15-year-old woman with ADTA who developed vitiligo with leukotrichia following topical DPCP immunotherapy. (Korean J Dermatol 2019;57(2):94∼96)

An In Vitro Study of the Antifungal Effect of Silver Nanoparticles on Oak Wilt pathogen Raffaelea sp.

Sang Woo Kim,Kyoung Su Kim,Kabir Lamsal,Young Jae Kim,Seung Bin Kim,Moo Young Jung,Sang Jun Sim,Ha Sun Kim,Seok Joon Chang,Jong Kuk Kim,Youn Su Lee 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.8

Effect of Chitosan Foliar Application on Soluble Sugars, Glucosinolates, and ABA catabolites Contents in ‘Chungwang’ Kimchi cabbage under Extreme and Moderate High Temperature

Ha Seon Sim,Ui Jeong Woo,Won Jun Jo,Hye Jin Lee,Yu Hyun Moon,Soo Bin Jung,Su Ran Ahn,Jung Su Jo,Sung Kyeom Kim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

Physiological disorder (calcium deficiency) of Kimchi cabbage occurred by high temperature and frequent precipitation, which leads to unexpected price fluctuations of commodities. This purpose of this study was to profile on soluble sugars, glucosinolates, and ABA catabolites contents of Kimchi cabbage leaves under extreme and moderate high temperature treatments and chitosan foliar application. At April 6, 2020, the ‘Chungwang’ Kimchi cabbage were transplanted into 625L plastic boxes filled with commercial and general soil at open-field in Wanju. Experiments were combined with three day/night temperature levels, 36/32°C (E; extreme high), 28/24°C (M; moderate high), and 20/16°C (C; control), and two chitosan foliar application (200g/L; and non chitosan foliar application). Temperature and chitosan foliar application were performed at 42 days after transplanting, and temperature treatments were maintained during 8 days in extreme weather growth simulators. The samples were collected in 4 and 8 days after commencing high temperature treatments. After 4 days of chitosan foliar application, the glucose and fructose contents of E treatment were 67.5 and 59.4 μg·g<SUP>-1</SUP>, respectively, which were higher than non application. In the chitosan foliar application treatment, the aliphatic (gluconapin and glucobrassicanapin) and indole (glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) glucosinolate contents of E treatment were 151.1, 342.2, 533.1, 1627, and 1807 μg·g<SUP>-1</SUP>, respectively, which were greater than non application. The ABA, DPA, and PA contents of E treatment under chitosan foliar application were 0.0114, 1.7, and 1.0 μg·g<SUP>-1</SUP>, respectively, higher than non application. After 8 days of chitosan folir application, the gluconapin, glucobrassicin, 4-methoxyglucobrassicin, and DPA contents of E treatment were 332.0, 445.8, 2037, 2.4 μg·g<SUP>-1</SUP>, respectively, which were greater than non application. There were no effects of chitosan foliar application in M and C treatments. Results indicated that the effect of chitosan foliar application is estimated to be approximately 4 days after commencing high tempearture treatment. The application of chitosan foliar increased ABA catabolites that relieve abiotic stress, it is considered to be effective in alleviating the high temperature damage of Kimchi cabbage.

Infliximab 치료 후 호전된 난치성 포도막염을 동반한 베체트병

최정우 ( Jeong Woo Choi ),최용준 ( Yong Joon Choi ),예영민 ( Young Min Ye ),김상하 ( Sang Ha Kim ),남동호 ( Dong Ho Nam ),박해심 ( Hae Sim Park ),이마빈 ( Ma Bin Lee ),유호민 ( Ho Min Yoo ),서창희 ( Chang Hee Suh ) 대한류마티스학회 2005 대한류마티스학회지 Vol.12 No.4

Behcet`s disease is a multisystemic, chronic relapsing inflammatory disease characterized by vasculitides, mucocutaneous lesions, thrombophlebitis, arthritis. Among them, the ocular involvement is usual (50∼70%) and the most serious morbidity is blindness (20∼25% of those of affected). Immunosuppressive treatment is currently the main therapy in chronic relapsing uveitis, but many patients experience recurrent sight-threatening uveitis. T lymphocytes play an important role in the pathogenesis of uveitis by releasing cytokines such as tumor necrosis factor (TNF) α. There are some reports that the anti-TNFα treatment is effective in refractory Behcet`s uveitis, but it has not been reported in Korea. We report a case of refractory uveitis in Behcet`s disease which is improved after anti-TNFα therapy.

Estimation of Evapotranspiration Model for Seasonal Irrigation Control of Garlic

Ui Jeong Woo,Won Jun Jo,Ha Seon Sim,Hye Jin Lee,Yu Hyun Moon,Soo Bin Jung,Su Ran Ahn,Jung Su Jo,Sung Kyeom Kim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

Irrigation amount and schedule is considered depend on the cultivation environments, cultural practices, crop species and it is necessary to estimate the water requirement accurately for efficient irrigation management. The purpose of this experiment is to estimate the amount of evapotranspiration of garlic (ETc) using the Penman-Monteith (P-M) equation in open field cultivation and to supply an appropriate amount of water per unit area for each season. The experiment was conducted from October 7, 2020 to June 16, 2021 at the open field of Kyungpook National University’s trial farm. Environmental data in terms of air temperature, wind speed, humidity, and solar radiation required for evapotranspiration amount (ETo) calculation were collected every 10 minutes in a data logger. The ETc was estimated by P-M equation. The average daily air temperature and relative humidity were maintained at 10.4°C and 52.7%, respectively during experiment periods. The daily average of ETo was approximately 5.9 mm and that increased at the late spring and the early summer cultivation season. When calculating seasonal ETo by four seasons based on 24 seasonal divisions, the daily averages of ETo were 6.07 mm, 4.92 mm, 6.42 mm, and 6.75 mm, respectively, autumn, winter, spring, and summer in sequence. ETo in winter was estimated to be lower than in other seasons, which is considered to be that crop transpiration was reduced due to dormancy. The ETc was calculated by multiplying ETo and the crop coefficient (Kc ini = 0.7, Kc mid = 1.0, and Kc end = 0.7). The seasonal average irrigation amount (ETc × cultivation area) per 10a was estimated 4.2 ton, 4.5 ton, 6.2 ton, and 4.7 ton, respectively. In addition, ETo increased from 200 days after transplanting, a period when the leaf senescence rate, bulb weight, and bulb diameter increased rapidly. Results founded that the seasonal irrigation amount of garlic estimated using the P-M equation can be applied for precise irrigation control.

Determination of Optimal Growing Degree Days and Cultivars of Kimchi Cabbage for Growth and Yield during Spring Cultivation under Shading Conditions

Ha Seon Sim,Won Jun Jo,Hye Jin Lee,Yu Hyun Moon,Ui Jeong Woo,Soo Bin Jung,Su Ran Ahn,Sung Kyeom Kim 한국원예학회 2021 원예과학기술지 Vol.39 No.6

This study determined the optimal growing degree days (GDD) and cultivars of Kimchi cabbage (Brassica rapa L. ssp. pekinensis) for growth and yield during spring cultivation under shading condition. There were nine treatments, and the experiments were conducted with three GDD levels (899, 924, and 961) and three cultivars (‘Chungwang’, ‘Cheongna’, and ‘Cheongok’) under shading conditions. The process-based model of Kimchi cabbage was applied for growth and yield prediction using meteorological data regarding light, temperature, wind, precipitation, and humidity in several regions. The number of leaves of ‘Chungwang’ Kimchi cabbage was greater than that of ‘Cheongna’ and ‘Choengok’ at all planting times. Most of the growth parameters were highest in the delayed transplanting treatment than in the other transplanting times. ‘Chungwang’ Kimchi cabbage was less sensitive to high temperature during spring cultivation than the other cultivars. GDD 924 was the most optimal time to harvest under the shading condition. GDD 750-870 is considered an appropriate harvest time in the primary production regions during the spring cultivation season. If shading is performed in these regions, constant production may be possible, thus, alleviating damage from high temperature.