Affinity-Purification of Fibrinogenase with High Proteolytic Activity from Agkistrodon halys (Chinese) Venom

Ma, Biao,Zhan, Ying,Wu, Dan,Jia, Jianping,Xu, Wentao,Luo, Yunbo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.9

To purify and characterize the fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom. Monoclonal antibodies against fibrinogenase were prepared and a novel affinity chromatography equipped with a monoclonal antibody against fibrinogenase was developed and applied for the purification of fibrinogenases. The purified fibrinogenase was identified by fibrinolytic activity assay, and antithrombosis activity assay. HPLC chromatography and SDS-PAGE analysis demonstrated the uniformity and purity of the purified fibrinogenase. In comparison with a conventional A-50 chromatography method, affinity-purified fibrinogenase showed higher activity ($3631\;U\;mg^{-1}$ vs $501\;U\;mg^{-1}$). In addition, the physiological activity of the fibrinogenase both in vitro and ex vivo showed the purified fibrinogenase can specifically degrade $\beta$-, $\gamma$-fibrinogen and has a high anti-thrombotic activity. In conclusion, the purified fibrinogenase by affinity column were shown to be homogeneous and showed a high and specific proteolytic activity against $\beta$-chains of fibrinogen molecules and antithrombosis activity.

Affinity-Purification of Fibrinogenase with High Proteolytic Activity from Agkistrodon halys (Chinese) Venom

Biao Ma,Ying Zhang,Dan Wu,Jianping Jia,Wentao Xu,Yunbo Luo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.9

To purify and characterize the fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom. Monoclonal antibodies against fibrinogenase were prepared and a novel affinity chromatography equipped with a monoclonal antibody against fibrinogenase was developed and applied for the purification of fibrinogenases. The purified fibrinogenase was identified by fibrinolytic activity assay, and antithrombosis activity assay. HPLC chromatography and SDS-PAGE analysis demonstrated the uniformity and purity of the purified fibrinogenase. In comparison with a conventional A-50 chromatography method, affinity-purified fibrinogenase showed higher activity (3631 U mg-1 vs 501 U mg-1). In addition, the physiological activity of the fibrinogenase both in vitro and ex vivo showed the purified fibrinogenase can specifically degrade β-, γ-fibrinogen and has a high anti-thrombotic activity. In conclusion, the purified fibrinogenase by affinity column were shown to be homogeneous and showed a high and specific proteolytic activity against β-chains of fibrinogen molecules and antithrombosis activity.

Nonlinear Quality Indices Based on a Novel Lempel-Ziv Complexity for Assessing Quality of Multi-Lead ECGs Collected in Real Time

Yatao Zhang,Zhenguo Ma,Wentao Dong 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.2

We compared a novel encoding LempelZiv complexity (ELZC) with three common complexity algorithmsi.e., approximate entropy (ApEn), sample entropy (SampEn), and classic LempelZiv complexity (CLZC) soas to determine a satisfied complexity and its corresponding quality indices for assessing quality of multileadelectrocardiogram (ECG). First, we calculated the aforementioned algorithms on six artificial time series inorder to compare their performance in terms of discerning randomness and the inherent irregularity within timeseries. Then, for analyzing sensitivity of the algorithms to content level of different noises within the ECG, weinvestigated their change trend in five artificial synthetic noisy ECGs containing different noises at severalsignal noise ratios. Finally, three quality indices based on the ELZC of the multilead ECG were proposed toassess the quality of 862 real 12lead ECGs from the MIT databases. The results showed the ELZC coulddiscern randomness and the inherent irregularity within six artificial time series, and also reflect content levelof different noises within five artificial synthetic ECGs. The results indicated the AUCs of three quality indicesof the ELZC had statistical significance (>0.500). The ELZC and its corresponding three indices were moresuitable for multilead ECG quality assessment than the other three algorithms.

Transcriptome analysis reveals candidate genes for dietary fifi ber metabolism in Rosa roxburghii fruit grown under different light intensities

Xiaoying Zhang,Min Lu,Richard A. Ludlow,Wentao Ma,Huaming An 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.5

The fruit of the perennial rosebush Rosa roxburghii were valued for their high levels of ascorbic acid, superoxide dismutaseactivity, and cancer preventing eff ects. The high cellulose and low pectin content of Rosa roxburghii fruit results in an undesirablefi brous texture and hence needs to be addressed. However, little is known about the molecular mechanisms underlyingdietary fi ber metabolism in this fruit. Here, we report that the contents of cellulose, pectin, and lignin were increased byshading treatments at the maturation stage of fruit development. Under 50% shading, the soluble pectin content increased by16.39%, which may improve the fruit palatability. However, deeper shading of 100% caused the lignin content to increase by28.86%, which conversely may lower fruit quality. Based on transcriptome analysis, we identifi ed candidate genes involvedin dietary fi ber metabolism, including cellulose synthase (CesA) 1, 2, 3, and 5 , â-1,4-xylosyltransferase (IRX) , arabinosyltransferase(ARAD) 1 and 2 , galacturonosyltransferase (GAUT) , cellulolytic enzyme (Cx) , and pectin methylesterase , inwhich CesA1, CesA2, CesA3, IRX, ARAD2 , and GAUT3 signifi cantly responded to shading and positively correlated withthe content of their corresponding component. Furthermore, cinnamyl alcohol dehydrogenase was signifi cantly regulatedby shading treatment and positively correlated with increasing lignin concentration. These results may facilitate a betterunderstanding of the molecular mechanisms of dietary fi ber metabolism in R. roxburghii fruit under low light conditionsand provide a framework for future crop improvement.

Development and Application of Three-Dimensional Bioprinting Scaffold in the Repair of Spinal Cord Injury

Lu Dezhi,Yang Yang,Zhang Pingping,Ma Zhenjiang,Li Wentao,Song Yan,Feng Haiyang,Yu Wenqiang,Ren Fuchao,Li Tao,Zeng Hong,Wang Jinwu 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

Spinal cord injury (SCI) is a disabling and destructive central nervous system injury that has not yet been successfully treated at this stage. Three-dimensional (3D) bioprinting has become a promising method to produce more biologically complex microstructures, which fabricate living neural constructs with anatomically accurate complex geometries and spatial distributions of neural stem cells, and this is critical in the treatment of SCI. With the development of 3D printing technology and the deepening of research, neural tissue engineering research using different printing methods, bio-inks, and cells to repair SCI has achieved certain results. Although satisfactory results have not yet been achieved, they have provided novel ideas for the clinical treatment of SCI. Considering the potential impact of 3D bioprinting technology on neural studies, this review focuses on 3D bioprinting methods widely used in SCI neural tissue engineering, and the latest technological applications of bioprinting of nerve tissues for the repair of SCI are discussed. In addition to introducing the recent progress, this work also describes the existing limitations and highlights emerging possibilities and future prospects in this field.

Mycobacterium vaccae induces a strong Th1 response that subsequently declines in C57BL/6 mice

Lijiao Zhang,Yanlong Jiang,Ziyin Cui,Wentao Yang,Limin Yue,Yingcong Ma,Shaohua Shi,Chun-fang Wang,Chun-feng Wang,Aidong Qian 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4

Mycobacterium (M.) vaccae is a fast-growing species of saprophytic bacteria that is widely distributed. To understand the host immuneresponses induced by M. vaccae isolated from bovine submaxillary lymph nodes, C57BL/6 mice were infected with reference strain M. bovisBacillus Calmette-Guérin (BCG) and isolated M. vaccae using intraperitoneal injections. Comparison of the bacterial replication and organpathology between M. vaccae and M. bovis BCG revealed that M. vaccae was more malignant than M. bovis in mice. We also demonstratedthat serum from the M. vaccae-infected mice contained a higher expression level of gamma-interferon (IFN-), tumor necrosis factor alpha,monocyte chemoattractant protein-1, interleukin (IL)-4, IL-12, IL-10 and transforming growth factor beta than did the other groups, especiallyafter week 4. Furthermore, when the numbers of CD3+CD4+IFN-+ and CD3+CD4+IL4+ cells in the infected mice were observed by flowcytometry, we found that a powerful T helper 1 (Th1) response was induced by M. vaccae infection, which was associated with the emergenceof CD3+CD4+IFN-+ cells. However, the Th1 response declined over time, which was associated with appearance of the CD4+CD25+FoxP3+and CD4+CD25+CD152+Treg cell reaction. In addition, a strong Th2 response was found. Finally, we found that M. vaccae infection increasedthe production of type I IFNs, which was associated with a reduced Th1 response.

Hydrothermal Synthesis of Band Gap-Tunable Oxygen-Doped g-C3N4 with Outstanding "Two-Channel" Photocatalytic H2O2 Production Ability Assisted by Dissolution–Precipitation Process

Hui Wang,Yuanhao Guan,Shaozheng Hu,Yanbo Pei,Wentao Ma,Zhiping Fan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.2

Here, band gap-tunable oxygen-doped graphitic carbon nitride (g-C3N4) with outstanding “two-channel” photocatalytic H2O2 production ability was prepared via hydrothermal treatment assisted by dissolution–precipitation process. XRD, N2 adsorption, UV–Vis, Fourier-transform infrared spectra, SEM, electrochemical impedance spectra, XPS and photoluminescence were used to characterize the obtained catalysts. The photocatalytic H2O2 production ability of as-prepared catalyst was investigated. The results show that oxygen doping not only changes the morphology of catalyst, decreases the band gap energy and promotes the separation efficiency of photogenerated electrons and holes, but also tunes the CB and VB potentials. As-prepared oxygen-doped g-C3N4 displays a H2O2 concentration of 3.8 mmol L -1, more than 7.6 times higher than that of neat g-C3N4. Because of the shift of CB and VB potentials, not only the CB electrons of oxygen-doped g-C3N4 reduce O2 to form H2O2, but also the VB holes can oxidize OH- to form OH, which subsequently react with each other to form H2O2. Such “two-channel pathway” causes the remarkably promoted H2O2 production ability.

454 Pyrosequencing Analysis on Microbial Diversity of an Expanded Granular Sludge Bed Reactor Treating High NaCl and Nitrate Concentration Wastewater

Runhua Liao,Yan Li,Zhu Wang,Yu Miao,Ke Shen,Peng Shi,Yan Ma,Wentao Li,Aimin Li 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

The treatment of high-salinity, high-nitratewastewater was investigated in a single expanded granularsludge bed reactor. Complete denitrification was achievedwhen nitrate concentration was as high as 6,000 mg N/Land the salinity of influent reached 11% NaCl at liquidup-flow velocity of 3.0 m/h, hydraulic retention time of,24 h and the C/N molar ratio of 2.0. Furthermore, 454-pyrosequencing technology was used to analyze archaeabacterial diversity under high salinity and high nitrateconditions. Results showed that the total number ofeffective sequences was 5749 consisting of 5678 bacterialsequences and 71 archaea sequences after denoising andfiltering out chimeras, which could be affiliated to 5phylogenetic groups, including Proteobacteria, Firmicutes,Euryarchaeota, Crenarchaeota and unclassified phylum. Although Proteobacteria was the dominant microbialpopulation, two archaea phylogenetic groups—Crenarchaeotaand Euryarchaeota were observed in this study.