MUC16 facilitates cervical cancer progression via JAK2/STAT3 phosphorylation‑mediated cyclooxygenase‑2 expression

Hui Shen,Meng Guo,Lu Wang,Xinyue Cui 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Objectives MUC16 (mucin 16, also known as CA-125, cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) has been predicted as tumor biomarker for therapy. We determined to investigate effects and regulatory mechanism of MUC16 on cervical tumorigenesis. Methods Expression levels of MUC16 in cervical cancer cell lines was analyzed via qRT-PCR (quantitative real-time polymerase chain reaction). Knockdown of MUC16 was conducted via shRNA (Short hairpin RNA) transfection. MTT and colony formation assays were used to investigate effect of MUC16 on cell proliferation. Wound healing assay was utilized to detect migration and transwell assay to detect invasion. The underlying mechanism was demonstrated via western blot analysis. Results MUC16 was elevated in cervical cancer cell lines. MUC16 knockdown inhibited cell proliferation, invasion and migration. Gain- and loss-of functional assays revealed that over-expression of MUC16 activated Janus Kinase 2 (JAK2)/ signal transducer and activator of transcription 3 (STAT3) via phosphorylation, thus facilitating cyclooxygenase-2 (COX-2) expression, while knockdown of MUC16 demonstrated the reverse effect on JAK2/STAT3 activation and COX-2 expression. Moreover, inhibition of JAK2/STAT3 attenuated the regulation of MUC16 on COX-2. Conclusions MUC16 enhanced proliferation and invasion of cervical cancer cells via JAK2/STAT3 phosphorylation-mediated cyclooxygenase-2 expression, suggesting the potential therapeutic target ability of MUC16 to treat cervical cancer.

Production of a Recombinant Laccase from Pichia pastoris and Biodegradation of Chlorpyrifos in a Laccase/Vanillin System

( Hui fang Xie ),( Qi Li ),( Min Min Wang ),( Lin Guo Zhao ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6

The recombinant strain P. pastoris GS115-lccC was used to produce laccase with high activity. Factors influencing laccase expression, such as pH, methanol concentration, copper concentration, peptone concentration, shaker rotate speed, and medium volume were investigated. Under the optimal conditions, laccase activity reached 12,344 U/L on day 15. The recombinant enzyme was purified by precipitating and dialyzing to electrophoretic homogeneity, and was estimated to have a molecular mass of about 58 kDa. When guaiacol was the substrate, the laccase showed the highest activity at pH 5.0 and was stable when the pH was 4.5~6.0. The optimal temperature for the laccase to oxidize guaiacol was 60℃, but it was not stable at high temperature. The enzyme could remain stable at 30℃ for 5 days. The recombinant laccase was used to degrade chlorpyrifos in several laccase/mediator systems. Among three synthetic mediators (ABTS, HBT, VA) and three natural mediators (vanillin, 2,6-DMP, and guaiacol), vanillin showed the most enhancement on degradation of chlorpyrifos. Both laccase and vanillin were responsible for the degradation of chlorpyrifos. A higher dosage of vanillin may promote a higher level of degradation of chlorpyrifos, and the 2-step addition of vanillin led to 98% chlorpyrifos degradation. The degradation of chlorpyrifos was faster in the L/V system (kobs = 0.151) than that in the buffer solution (kobs = 0.028).

Effects of Quenching Methods and Aging Processes on the Crushing Properties and Microstructure of Al–Zn–Mg Alloy Thin-Walled Square Extrusions

Hui Guo,Jin Zhang,Cheng Wang,Yun‑lai Deng 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

In this study, the effects of different (online quenching and offline quenching) quenching methods and aging processes (T6and T73) on the crashworthiness and microstructural evolution of three Al–Zn–Mg alloys (G1–G3) were studied by conductingtensile test and axial compression tests at room temperature, combined with optical metallography, electron back scattereddiffraction, and transmission electron microscopy microstructural observations. The obtained results revealed that thecrushing properties of three different Al–Zn–Mg alloys subjected to different quenching methods and aging processes weresignificantly different. Their crushing energy absorption of are ranked as follows: G1 > G3 > G2. The highest total energyabsorption gap (between T6 and T73) is the G1 alloy, and the lowest one is the G3 alloy. The largest total energy absorptiongap between the two quenching methods is the G3 alloy, and the smallest one is the G1 alloy. The G2 alloy with the largesttotal amount of Zn + Mg has the highest number density of matrix precipitates, the largest precipitate gap (between T6 andT73) and the smallest precipitate gap between the two quenching methods. The G3 alloy with the largest Zn/Mg ratio hasthe smallest number density of matrix precipitates, the minimum precipitation gap (between T6 and T73) and the maximumprecipitation gap between the two quenching methods. The G1 alloy with the lowest Zn/Mg ratio has the smallest size ofgrain boundary precipitates and PFZ width, while their largest values are obtained for the G3 alloy with the maximum Zn/Mg ratio. As a crushing resistant structural material, the crushing properties is improved without reducing the strength. Theratio of Zn/Mg should be controlled within the range of 4.57–6.15, while the total amount of Zn + Mg should be controlledwithin the range of 6.18–7.01.

A novel design method for improving collapse resistances of multi-story steel frames with unequal spans using steel braces

Zheng Tan,Wei-hui Zhong,Bao Meng,Shi-chao Duan,Hong-chen Wang3,Xing-You Yao,Yu-hui Zheng 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.2

The bearing capacities resisted by the two-bay beams of multi-story planar frames with unequal spans under column removal scenarios differ considerably owing to the asymmetric stress on the left and right beams connected to the failed column and cause the potential for beams with larger span-to-depth ratios to be unable to exert effectively, which is disadvantageous for resisting the vertical load in unequal-span frame structures. To address this problem, the structural measure of adding braces to the weak bays of multi-story unequal-span frames was proposed, with the objective of achieving a coordinated stress state in two-bay beams with unequal spans, thereby improving the collapse resistance of unequal-span frame structures. Before conducting the numerical simulation, the modeling methods were verified by previous experimental results of two multi-story planar frames with and without steel braces. Thereafter, the effects of the tensile and compressive braces on the collapse behavior of the frame structures were elucidated. Then, based on the mechanical action laws of the braces throughout the collapse process, a detailed design method for improving the collapse resistance of unequal-span frame structures was proposed. Finally, the proposed design method was verified by using sufficient example models, and the results demonstrated that the design method has good application prospects and high practical value.

흡착 우라늄 이온의 전기 탈착거동

정종헌,원휘준,최왕규,김계남,이성호,오원진 한국공업화학회 2003 응용화학 Vol.7 No.1

Liquid wastes containing uranium ions in high concentration of chemical salts havebeen treated by electrosorption on a activated carbon fibers(ACFs) electrode. Effectiveuranium (VI) removal is accomplished when the potential is -0.5V(vs. Ag/AgCl) ormore negative. For a feed concentration of 100mg/1, the concentration of U(VI) in thecell effluent is reduced to less than 1mg/1, and electrosorption capacity over 500mg/uranium/gACF is reached. The adsorbed uranium can be desorbed up to 99%for 20h by passing a 1.9N NaCl solution at pH 3 through the cell and applying a potential of +1.2V. The amount of desorbed uranium increased as the solution pHwas lowered and the applied potential increased.

Inhibition of Microcystis aeruginosa by the Extracellular Substances from an Aeromonas sp

( Yu Mei Liu ),( Ming Jun Chen ),( Meng Hui Wang1 ),( Rui Bao Jia ),( Li Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9

Growth of Microcystis aeruginosa could be inhibited significantly within 24 h by the extracellular substances prepared from Aeromonas sp. strain FM. During the treatment, the concentration of extracellular soluble carbohydrates increased significantly in algal culture. Morphological and ultrastructural changes in M. aeruginosa cells, including breakage of the cell surface, secretion of mucilage, and intracellular disorganization of thylakoids, were observed. HPLC-MS analysis showed that the extracellular substances of Aeromonas sp. strain FM were a mixture of free amino acids, tripeptides, and clavulanate. Among these, the algaelysis effects of lysine and clavulanate were confirmed.

개에서 발생한 이버멕틴 중독증 1례

여동원,윤기영,박정호,이왕희,송근호,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 1-year old, intact male Jin-do dog with ataxia, hypersalivation and anorexia was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Ivermectin toxicosis was diagnosed by the history taking, clinical signs, physical examination and neurological examination. After therapy with intravenous fluid and lipid emulsion administration, the clinical signs were found to have significantly improved.

개 아토피성 피부염 장기치료 1례

박은정,윤기영,박정호,이왕희,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 7-year-old male castrated Shih-tzu dog was referred to the Veterinary Medical Teaching Hospital of Chungnam National University for severe pruritus. On physical examination were confirmed systemic erythema and alopecia, crust on the neck and flank region. Tape strip test identified numerous degenerative neutrophils and severe Malassezia infection. Treatment for severe pruritus was applied with systemic antihistamine therapy and topical therapy with antimicrobial shampoos. For the finding of appropriate treatment of atopic dermatitis, it was chosen six immune-related treatments. And clinical sign scores were evaluated by investigator with CADESI-03 after each application of six treatments. Application of human immunoglobulin G and leflunomide showed the remarkable regression of lesions.

고양이의 만성 췌장염 치료 증례

김현아,윤기영,박정호,이왕희,송근호,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 5-year-old Russian blue cat was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of anorexia, lethargy, weight loss(6.5 to 4.8 kg) 2 weeks ago. Abdominal ultrasonography showed the following : increase of echogenicity of liver, irregular shape of gall bladder, hyperechoic region around duodenum. Pancreatitis, cholangitis, hepatic lipidosis was diagnosed by the ultrasonography. Anti- inflammatory therapy, antibiotic therapy was administered. Abdominal ultrasonography is a efficient available diagnostic tool but serum fPL test reveals the highest sensitivity and specifity result for feline pancreatitis. SNAP fPL test is newly released that can check pancreatitis within 10 minutes by same princple of Spec fPL test used in Gastrointestinal Laboratory of Texas A & M Univerrsity.

Exploring the Impact of a STEM Integration Teacher Professional Development Program on Secondary Science and Mathematics Teachers’ Perceptions of Engineering and Their Attitude toward Engineering Integrated Teaching

Hui-Hui Wang,남윤경 한국지구과학회 2015 韓國地球科學會誌 Vol.36 No.5

This study explores the impact of a STEM integration teacher professional development program focusing on teachers’ perception of engineering and their attitudes toward integrating engineering into teaching. A total of sixty-eight teachers from ten schools participated in the program for five days. Data are collected from three main sources including (1) pre and post concept maps probing teachers’ perceptions about the engineering discipline, (2) a pre and post survey measuring teachers’ self-efficacy of teaching science/mathematics within the engineering context, and (3) engineering integrated science and (or) mathematics lesson plans and teaching reflections. This study utilizes both qualitative and quantitative research methods depending on the data we have collected. The results show that both science and math teachers thought that integrating engineering into teaching provided valuable outcomes, i.e., promoting students’ learning about engineering and improving their interest in science or math through real-world problem solving exercises. Participants also felt more comfortable about integrating engineering in their teaching after the program. The results also imply that the teachers’ understandings of engineering become more concrete after the program. This study also provides an overview of the challenges and advantages of teaching engineering in K-12 science and mathematics classrooms.