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Wajima Safi,Andreas Kuehnl,Andreas Nussler,Hans-Henning Eckstein,Jaroslav Pelisek 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-
The aim of this study was to determine the optimal culturing media for human CD14+ monocytes and to evaluate whether these cells are capable of differentiating into vascular endothelial cells. Human monocytes isolated from peripheral blood were cultured for 1, 3, 7, 10 or 14 days in different media containing either 10% fetal bovine serum (FBS), 10% autologous donor serum (Auto), 10% FBS with interleukin-3 and macrophage colony stimulating factor (FBS-WF) or 10% Auto and the same growth factors (AU-WF). The cells were differentiated using endothelial cell conditioning medium (EC). Viability was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the cells were characterized by histology, immunohistochemistry and western blot analysis. Monocytes treated with Auto, FBS-WF or AU-WF medium generated a significant higher yield of vital cells after 7 days in culture compared with FBS-only medium (mean difference (MD) = 0.318, P = 0.01; MD = 1.83, P = 0.04; or MD = 0.271, P = 0.01 and MD = 0.318, P = 0.102). All tested media led to the differentiation of monocytes into macrophages, identified by CD68, especially in the FBS-WF medium (MD = +18.3%; P = 0.04). Differentiation into ECs caused a significant decrease in cell viability in all media. Endothelial cell markers, including CD31, CD144, VEGF, VEGF-R2 and CD34, could not be detected. Autologous serum significantly increases the yield of monocytederived cells with a higher effectiveness than commonly used FBS-only serum. There is no further benefit in culturing monocytes longer than 7 days. The cultivation of monocytes in the tested media leads preferentially to differentiation into macrophages. Differentiation into endothelial cells did not take place.
The East-Asian VLBI Network: Recent Progress and Results of the First Imaging Test Observation
Kiyoaki Wajima,Duk-Gyoo Roh,Se-Jin Oh,Taehyun Jung,Jongsoo Kim,Yoshiaki Hagiwara,Kazuhiro Hada,Noriyuki Kawaguchi,Hideyuki Kobayashi,Yuanwei Wu,Kenta Fujisawa,Tao An,Willem A. Baan,Wu Jiang,Zhi-Qiang 한국천문학회 2016 天文學會報 Vol.41 No.1
Intraday Variability Feature and Milliarcsecond-Scale Structure in PKS 1622-297
Kiyoaki Wajima,Hayley E. Bignall,Hideyuki Kobayashi,Hisashi Hirabayashi,Kenta Fujisawa,Masato Tsuboi,Philip G. Edwards,Yasuhiro Murata 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.4
PKS 1622.297 is the most luminous gamma-ray emitting active galactic nucleus (AGN) ever detected and shows a gamma-ray intraday variability (IDV). We made a high-resolution space very long baseline interferometry (VLBI) observation of the source almost three years after the gammaray IDV. The source shows a compact core-jet structure, and all jet components have an apparent superluminal motion up to 12 h.1c. As an alternative probe of the sub-parsec scale structure, we also present the results from multi-epoch Australia Telescope Compact Array (ATCA) total flux monitoring, which indicate the presence of IDV. We examine the inner structure of the gamma-ray emitting AGN in light of these observations.
A FANAROFF-RILEY TYPE I CANDIDATE IN NARROW-LINE SEYFERT 1 GALAXY Mrk 1239
Doi, Akihiro,Wajima, Kiyoaki,Hagiwara, Yoshiaki,Inoue, Makoto IOP Publishing 2015 ASTROPHYSICAL JOURNAL LETTERS - Vol.798 No.2
<P>We report finding kiloparsec-scale radio emissions aligned with parsec-scale jet structures in the narrow-line Seyfert 1 (NLS1) galaxy Mrk 1239 using the Very Large Array and the Very Long Baseline Array. Thus, this radio-quiet NLS1 has a jet-producing central engine driven by essentially the same mechanism as that of other radio-loud active galactic nuclei (AGNs). Most of the radio luminosity is concentrated within 100 parsecs and overall radio morphology looks edge-darkened; the estimated jet kinetic power is comparable to Fanaroff-Riley Type I radio galaxies. The conversion from accretion to jet power appears to be highly inefficient in this highly accreting low-mass black hole system compared with that in a low-luminosity AGN with similar radio power driven by a sub-Eddington, high-mass black hole. Thus, Mrk 1239 is a crucial probe to the unexplored parameter spaces of central engines for a jet formation.</P>