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Ha Thi Thanh Tran,Duc Anh Truong,Viet Duc Ly,Hao Thi Vu,Tuan Van Hoang,Chinh Thi Nguyen,Nhu Thi Chu,Vinh The Nguyen,Duyen Thuy Nguyen,Kohtaroh Miyazawa,Takehiro Kokuho,Hoang Vu Dang 대한백신학회 2020 Clinical and Experimental Vaccine Research Vol.9 No.1
Purpose: To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease. However, the efficacy of these vaccines to protect the pig against field CSF strains needs to be considered, based on circulating strains of classical swine fever virus (CSFV). Materials and Methods: Recombinant E2-CSFV protein produced by baculovirus/insect cell system was analyzed by western blots and immunoperoxidase monolayer assay. The effect of CSFV-E2 subunit vaccines was evaluated in experimental pigs with three genotypes of CSFV challenge. Anti-E2 specific and neutralizing antibodies in experimental pigs were analyzed by blocking enzyme-linked immunosorbent assay and neutralization peroxidize-linked assay. Results: The data showed that CSFV VN91-E2 subunit vaccine provided clinical protection in pigs against three different genotypes of CSFV without noticeable clinical signs, symptoms, and mortality. In addition, no CSFV was isolated from the spleen of the vaccinated pigs. However, the unvaccinated pigs exhibited high clinical scores and the successful virus isolation from spleen. These results showed that the E2-specific and neutralizing antibodies induced by VN91-E2 antigen appeared at day 24 after first boost and a significant increase was observed at day 28 (p<0.01). This response reached a peak at day 35 and continued until day 63 when compared to controls. Importantly, VN91-E2 induced E2-specific and neutralizing antibodies protected experimental pigs against high virulence of CSFVs circulating in Vietnam, including genotype 1.1, 2.1, and 2.2. Conclusion: These findings also suggested that CSFV VN91-E2 subunit vaccine could be a promising vaccine candidate for the control and prevention of CSFV in Vietnam.
Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10
Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.
Van-Truc Nguyen,Thanh-Binh Nguyen,Thi-Dieu-Hien Vo,Nguyen Duy Dat,Thi-Kim Quyen Vo,Xuan Cuong Nguyen,Viet-Cuong Dinh,Thi-Ngoc-Chau Le,Thi-Giang-Huong Duong,Manh-Ha Bui,Xuan-Thanh Bui 대한환경공학회 2023 Environmental Engineering Research Vol.29 No.3
This study explores the adsorption of doxycycline (DOX) from aqueous solutions onto biochar derived from banana peel, which was prepared using a potassium hydroxide activation method (KOH-BPB). The biochar properties were characterized based on morphology, surface area (SBET of 710.241 ㎡ g<SUP>−1</SUP>), functional groups, and surface charge (pHPZC = 7.7). Parameters, including initial pH, DOX concentration, and ionic strength, that influenced the DOX adsorption capacity of KOH-BPB were examined. Adsorption equilibrium of DOX on KOH-BPB was assessed through four isothermal models: the Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich models. The obtained data were most compatible with the Langmuir model (R² = 0.9879). KOH-BPB has a maximum DOX absorption capacity of 121.95 mg g<SUP>-1</SUP> which exceeds that of many comparable absorbents. The maximum DOX removal was 96.7% at pH 6, a DOX concentration of 20 mg L<SUP>-1</SUP>, and a KOH-BPB dose of 1.0g L<SUP>-1</SUP>. These findings reveal that biochar from banana peel effectively removes antibiotic residues from water. This study provides a potential, low-cost, and environmentally friendly adsorbent.
Artocarpus nigrifolius: Cytotoxic and Antibacterial Constituents
Hoi, Tran Minh,Anh, Ha Van,Huong, Nguyen Thi Thanh,Tuyen, Nguyen Van,Anh, Le Thi Tu,Tra, Nguyen Thanh,Cham, Ba Thi,Ha, Nguyen Thi Thu,Linh, Pham Thuy,Tien, Doan Duy,Kiem, Phan Van,Ban, Ninh Khac,Kukha The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6
Six known compounds including ${\alpha}$-amyrin 3-acetate (1), ${\beta}$-sitosterol (2), betulinic acid (3), friedelan-3-one (4), artochamin B (5), and 2-C-methyl-D-erythritol 4-O-${\alpha}$-D-glucopyranoside (6) were isolated from the stem barks and leaves of Artocarpus nigrifolius (Moraceae) for the first time. Their structures were identified by spectroscopic methods as well as comparison with literatures. Cytotoxicity and antibacterial activity of 1-6 were evaluated. Results showed that artochamin B (5) possessed the highest cytotoxicity towards MCF7, Lu, HepG2, and KB cell lines with $IC_{50}$ values of 4.59, 20.00, 3.60, and $1.18{\mu}g/mL$, respectively. It also inhibited the growth of Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus), whereas inactive on the growth of both Gram-negative bacteria and yeast.
Artocarpus nigrifolius: Cytotoxic and Antibacterial Constituents
Tran Minh Hoi,김영호,Ha Van Anh,Nguyen Thi Thanh Huong,Nguyen Van Tuyen,Le Thi Tu Anh,Nguyen Thanh Tra,Ba Thi Cham,Nguyen Thi Thu Ha,Pham Thuy Linh,Doan Duy Tien,Phan Van Kiem,Ninh Khac Ban,Lidziya Kukha 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6
Six known compounds including α-amyrin 3-acetate (1), β-sitosterol (2), betulinic acid (3), friedelan-3-one (4),artochamin B (5), and 2-C-methyl-D-erythritol 4-O-α-D-glucopyranoside (6) were isolated from the stem barks and leaves of Artocarpus nigrifolius (Moraceae) for the first time. Their structures were identified by spectroscopic methods as well as comparison with literatures. Cytotoxicity and antibacterial activity of 1-6 were evaluated. Results showed that artochamin B (5)possessed the highest cytotoxicity towards MCF7, Lu, HepG2,and KB cell lines with IC50 values of 4.59, 20.00, 3.60, and 1.18μg/mL, respectively. It also inhibited the growth of Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus), whereas inactive on the growth of both Gram-negative bacteria and yeast.
NGUYEN, Xuan Truong,NGUYEN, Thai Ha,DANG, Huynh Phuong,PHAM, Thi Lan Phuong,BUI, Thi Thanh,TRAN, Nhat Minh,HUYNH, Tri An,NGUYEN, Nam Phong Korea Distribution Science Association 2022 유통과학연구 Vol.20 No.2
Purpose: This study aims to identify factors affecting the adoption of e-commerce by household businesses in Vietnam's food and beverage industry. Research design, data and methodology: The integrated TAM and TOE frameworks and ten hypotheses were developed to test the relationship between relevant factors. A cross-sectional survey was conducted to collect valid data from 992 respondents who run F&B household businesses in Vietnam. Results: The empirical research results confirm all proposed hypotheses that e-commerce adoption is affected by the perceived usefulness, the perceived ease of use, vendor support partners, social expectancy, competitive pressure, subjective norm, and household resources. Meanwhile, technical readiness, environment readiness, and organization readiness are intermediate variables that influence the implementation of e-commerce in food and beverage distribution. Conclusions: The findings suggest effective orientations to foster the food and beverage e-trading practice for household businesses in developing countries. Accordingly, to encourage private household businesses to adopt e-commerce, it is necessary to focus on improving business resources, technology readiness, environment readiness, and organization readiness through raising awareness of usefulness, benefits, perceived ease of use, and increased support of vendor partners. Future research can focus on improving the efficiency of e-commerce applications in F&B distribution for both household businesses and larger-scale enterprises.