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강신몽,이원태,고영창,최상규,김윤희,이홍석,서재관,윤중진,이혜경,최득린,김종열,윤창육,변명식,이장홍 大韓法醫學會 1991 대한법의학회지 Vol.15 No.2
Individual identification is an important part in medicolegal field especially in mass disaster. At July, 27, 1989, KAL KE-803 was crashed on landing at Tripoli International Airport, Liba. The plane was caught in fire and sixty eight Koreans were sacrified. The majority of victims were severely charred and injured. The authors examed all dead bodies and successfully identified all the cases through visual, anthropological, odontological, radiological and pathological methods including fingerprint and blood typing.
ELISA 를 이용한 돼지 톡소플라스마병의 조기진단에 관한 연구
서명득,주후돈,장동화 경상대학교 축산진흥연구소 1989 畜産振興硏究所報 Vol.16 No.1
To investigate the influencce of retined placenta on the subsquent reproductive performance and milk yield, total 357 heads of Holstein dairy cows at N.A.B.I., Choong Nam province were used. The cows not expelled their placenta until 12 hours postparturiont were termed as the cow of placenta retention. The days to first service, days open, services per conception, lactation days, peak yield per day, days to peak yield and 305 days ME nilk yied were evaluated to determine the subsequent reproductive performances and milk yield, respectively. The mean incidence of retaincd placenta(RP) was 25.8% in a herd of 357 cows examined. The cows of placenta retention took significantly(P$lt;0.01) longer than normal cows in the days to first service and the days open, by 97.6±8.4 days and 192.6±15.0 days versus 79.3±2.3 days and 142.9±5.5 days, respectively, but services per conception and days open more than 300 were not affected by retained placonta. In milk yield, lactation days, peak yield per day and days to peak yield were not affected by retained placenta, but milk yield per lactation was significantly(P$lt;0.05) decreased in RP cows with 5.684t151.9kg versus 6,035t80.Okg for normal cows. From these results, there scemed to be negative relationship between incidence of retained placenta and subsequent reproductive performance and /or milk vied.
재래흑산양(在來黑山羊)의 흡충류(吸蟲類)(간질(肝蛭), 췌질(膵蛭), 쌍구흡충(雙口吸蟲))에 대(對)한 Nitroxynil(Trodax)의 구충효과(驅蟲效果)
서명득,Suh, Myung-Deuk 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.2
The anthelmintic efficiency of nitroxynil(Trodax) at a dose rate of 10mg/kg was tested in naturally infected Korean native goat (Black goat) against Fasciola hepatica, Eurythrema pancreaticum, and Paramphistomum sp. The drug was administered with injection subcutaneouslly and the efficiency was measured by faecal examination microscopically. The results obtained were summarized as follows: 1. The anthelmintic efficiency of nitroxynil against Fasciola hepatica was shown 33.3% on day 7, 88.9% on day 14 and 94.4% on day 28 after the administration of the drug, respectively. The reduction rates of egg per gram of faeces (E. P. G.) against Fasciola hepatica in faecal examination were shown 86.8% on day 7, 97.7% on day 14, and 99.7% on day 28 postmedication. 2. The anthelmintic efficiency of nitroxynil against Paramphistomum sp. was shown 0% on day 7, 10.5% on day 14, and 36.8% on day 28 after the administration of drug. The reduction rates of E. P. G. against the parasite were shown 13.2% on day 14, and 39.4% on day 28 postmedication. 3. The anthelmintic efficiency of nitroxynil against Eurythrema pancreaticum was shown 0% on day 7, 16.6% on day 14, and 25% on day 28 after the administration of the drug.
Babesia ovata의 순수분리와 형태학적 및 혈청학적 동정
서명득,Suh, Myung-deuk 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2
This study was conducted to identify Babesia sp. strain isolated from the imported beef cattle, Aberdeen-angus heifers, in J farm of Jangsu Prefecture, Cheon-buk Province of Korea, morphologically, etiologically and serologically. Babesia sp. strain was purely isolated through the serial blood passages of three generations against splenectomized calves and one generation of blood passage against non-splenectomized calf(intact calf) by inhibiting the appearance of Theileria sergenti in the blood stream by means of injection of 20% oil pamaquine intramuscularly. In the splenectomized calves, the parasite multiplied markedly in blood stream soon after inoculation and parasitaemia was much severe. An elevated body temperature, anorexia, severe anaemia and icterus were observed clinically. Of three splenectomized calves, two were affected with haemoglobinuria and died. But in the non-splenectomized calf the clinical signs and parasitaemia were very mild. The means of the incubation period, the highest parasitaemia, the highest body temperature and the lowest PCV were 3.5 days, 41.1%, ${42^{\circ}C}$ and 9%, respectively, in the splenectomized calves. In calf erythrocytes Babesia sp. protozoa were polymorphic showing the round, oval, ameboid, piriform and paired piriform etc. The sizes of protozoa were $2.51{\sim}3.91{\times}1.32{\sim}2.19{{\mu}m}$ ($3.20{\times}1.60{\mu}m$). Serologically the isolated Babesia sp. were compared with other parasites, B. ovata, B. bigemina, B. bovis, T. sergenti, A. marginale and A. centrale by using the complement fixation(CF) test. As a result, the antibody titer between the homologous species were high. It was two tubes or more in the CF test. From the results obtained above the pure isolated Babesia species was identical with Babesia ovata Minami and Ishihara, 1980.
반추수(反芻獸)의 내부기생충(內部寄生蟲)에 대한 Albendazole과 Ivermectin의 구충효과(驅蟲效果)
서명득,Suh, Myung-deuk 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.2
The anthelmintic efficacy of Albendazo1e and Ivermectin against gastrointestinal nematodes, trematodes and cestode was tested in naturally infected Korean native goats. Albendazole was medicated at a dose rate of 10mg/kg of body weight orally and Ivermectin was injected at a dose rate of 0.2mg/kg of body weight subcutaneously. The efficacy was measured by faecal examinations on the day 7th and the day 14th :after treatment. The results obtained were summarized as follows : 1. The efficacy of Albendazole against trematodes and cestode was shown 91.7% in Fasciola hepatica, 68.8% in Paramphislomum spp., 66.7% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 2. The efficacy of Ivermectin against trematodes and cestode was shown 38.8% in Fasciola hepalica, 26.1% in Paramphistomum spp., 22,2% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 3. The anthelmintic efficacy of Albendazole against gastrointestinal nematodes was shown 97.7% in Haemonchus contorus and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Trichostrongylus spp., Strongyloides papillosus and Cooperia spp. 4. The efficacy of Ivermectin against gastrointestinal nematodes was shown 96.6% in Haemonchus contortus, 94.1% in Trichostrongylus spp. and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Strongyloides papillosus and Cooperia spp.
재래흑산양의 흡충류(간질, 쌍구흡충, 췌질)와 조충에 대한 Albendazole의 구충효과 시험
서명득,Suh Myung-deuk 대한수의사회 1984 대한수의사회지 Vol.20 No.10
The anthelmintic efficiency of Albendazole(Valbazen bolus) at a dose rate of 10 mg/kg of body weight was tested in naturally infected Korean native goat (Black goat) against Fasciola hepatica, Paramphistomum sp., Eurytrema pancreaticum and Moniezia expansa.
Polymerase chain reaction을 이용한 실험적 감염 돼지의 혈액과 조직으로부터 Toxoplasma gondii 검출
신명득,신기욱,Suh, Myung-deuk,Shin, Gee-wook 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.1
This study was conducted to detect the toxoplasma specific-DNA in circulating blood and organs collected from slaughtered pigs at slaughtering house and experimentally infected pigs with Toxoplasma gondii tachyzoites by polymerase chain reaction(PCR), and also PCR was applied to diagnose for acute phase of swine toxoplasmosis as a newly developed diagnostic test. The sensitivity of oligonucleotide primer, T-1 & T-2, designed from toxoplasma B1 gene amplification method was compared with Tp parasite detection by mouse inoculation(MI). On the other hand, latex agglutination test(LAT) was conducted to detect the serum antibodies comparing with the detection of toxoplasma by PCR and MI. The results obtained were summarized as follows. PCR was able to determine at the lowest level of $10^0/ml$ T. gondii in blood samples which were blended with a serial diluted T gondii in vitro. On the other hand, $10^2/5g$ of T gondii could detect from a variety of tissues including lung, diaphragm, liver, heart, spleen and brain in vitro. The primer was proved to specifically determine T gondii in blood and tissues in vitro but it did not detect Neospora caninum used as a negative control. DNA of T. gondii was effectively extracted by freezing, thawing and grinding twice both tissues mixed with T gondii in vitro and in experimentally infected pig's tissues. PCR detected specific DNA in the blood of experimentally infected pigs at 108 hrs and 120 hrs post-infection, it was the same time that the pigs showed fever and parasitaemia. In case of tissue, specific DNA was, however, detected only lung from experimentally infected pigs. Even though the duration of acute phase was from 3 to 7 days post-infection, but the latex agglutination test (LAT) results appeared from 8 days post-infection. A comparison of sensitivity in determining T gondii in blood samples between PCR and MI, PCR positive rate ranged from 25 to 33.3%, but that of MI covered from 75 to 100%.