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( Gui Hua Quan ),( Sei Ryang Oh ),( Hyuk Hwan Song ),( Hyeong Kyu Lee ),( Young Won Chin ) 한국응용생명화학회(구 한국농화학회) 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.
비장, 골수세포 및 대식세포에서의 Macrophage Inflammatory Protein-1α (MIP-1α)에 관한 연구
송인택,김지혜,유강열,김형섭,오귀옥 全北大學校 齒醫學硏究所 1992 전북치대논문집 Vol.10 No.1
The Macrophage inflammatory protein-1α(MIP-1α) from activated T cell or macrophage, which is small inducible cytokine of unknown biological function, has been shown to display inflammation chemokinetic activities, as well as myelosuppressive effect on more immature progenitor cells. In this paper we show the MIP-1α mRNA expression and the presence of MIP-1α binding sites from murine macrophage cell line RAW 264.7, but not inhibited by cyclosporin A treatment, and also was expressed from mouse splenocyte and bone marrow cell which were not increased by ferritin or lactoferrin treatment. The results of receptor binding assay showed that radiolabeled RAW 264.7 cell with kd value of 0.91 nM, and binding sites per cell of 378. Bone marrow cell and splenocyte also appealed to have MIP-1α binding sites 33 and 11 per cell, respectively.
오련,송은희,박기호,조오현,김탁,윤귀준,손병석,성흥섭,김미나,김양수,우준희 대한감염학회 2008 감염과 화학요법 Vol.40 No.5
Cryptococcosis is a disseminated infection caused by Cryptococcus neoformans. It usually causes pulmonary infection and meningitis in immunocompromised patients. However, osteomyelitis due to C. neoformans is extremely rare. It is generally known that cryptococcal osteomyelitis is a complication of disseminated cryptococcosis, appearing in 5-10%. We experienced a case of cryptococcal osteomyelitis with paraspinal abscess in a liver transplant patient.
A Note on the Lichen Genus Ramalina (Ramalinaceae, Ascomycota) in the Hengduan Mountains in China
( Ok Oh Soon ),( Yu Wang Xin ),( Song Wang Li ),( Gui Liu Pei ),( Seoun Hur Jae ) 한국균학회 2014 Mycobiology Vol.42 No.3
On the basis of extensive field investigation and a series of herbarium specimen identifications, we present and discussthe descriptions and distribution of 22 species of Ramalina found in the Hengduan Mountains of southwestern China. In this revisionary study, representatives of the Ramalina genus, including R. americana, R. confirmata, R. dendriscoides, R. obtusata, R. pacifica, R. pentecostii, R. peruviana, R. shinanoana, and R. subcomplanata are found for the first time in this area. In addition, R. holstii is reported for the first time China. Finally, a newly described species identified as Ramalina hengduanshanensis S. O. Oh &L. S. Wang is reported. It is characterized as growing from a narrow holdfast, solid, sparsely or richly and irregularly dichotomously branched, palmate and flattened lobes with distinctly dorsiventral appearance, surface rugose to reticulate, surface rugosely cracked, dense chondroid tissue, helmet shaped soralia at the tip. The species grows on rock and tree at the highest elevations inthis area. Although very few lichen species belonging to the genus Ramalina have been collected above 4,000 m, this new species is found at this elevation. We present detailed morphological, anatomical, and chemical descriptions of this species along with molecular phylogenetic analysis of the internal transcribed spacer rDNA sequences.
Quan, Gui-Hua,Oh, Sei-Ryang,Song, Hyuk-Hwan,Lee, Hyeong-Kyu,Chin, Young-Won The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.
Anti-allergic flavones from Arthraxon hispidus.
Quan, Gui-Hua,Chae, Hee-Sung,Song, Hyuk Hwan,Ahn, Kyoung-Seop,Lee, Hyeong-Kyu,Kim, Young-Ho,Oh, Sei-Ryang,Chin, Young-Won Pharmaceutical Society of Japan 2013 Chemical & pharmaceutical bulletin Vol.61 No.9
<P>Bioactivity-guided fractionation for an EtOAc-soluble fraction of methanolic extract of Arthraxon hispidus, using primary cell assay with bone marrow-derived mast cells (BMMC), led to an isolation of six new flavones and nine known compounds. The structures of the new compounds were established by one dimensional (1D)- and 2D-NMR spectroscopic data, as luteolin 8-C-β-kerriopyranoside (1), luteolin 8-acetic acid methyl ester (2), 7-methyl-luteolin 8-C-β-(6-deoxyxylo-3-uloside) (3), apigenin 8-C-α-fucopyranoside (4), apigenin 8-C-β-fucopyranoside (5) and luteolin 8-C-β-fucopyranoside (6). All the isolates were evaluated for inhibitory activities on interleukin-6 release in the primary cultures using BMMC. Of the tested compounds, compounds 2, 3 and 10 were found to inhibit interleukin-6 release. Furthermore, compound 2 displayed inhibitory activity against prostaglandin D2, leukotriene C4, and β-hexosaminidase releases.</P>
PNF 목 패턴이 전방머리자세를 가진 성인의 목 움직임 및 목 장애 지수에 미치는 영향
오현주,송귀빈,Oh, Hyun-Ju,Song, Gui-Bin 대한고유수용성신경근촉진법학회 2016 PNF and Movement Vol.14 No.3
Purpose: The purpose of this study was to investigate the effects of neck patterns in proprioceptive neuromuscular facilitation (PNF) for neck movement and the neck disability index (NDI) among adults with forward head posture. Methods: Thirty-nine subjects were randomly assigned into two groups. Subjects in the proprioceptive neuromuscular facilitation exercise group (PNFG, n = 20) received 20 minutes of PNF neck pattern (flexion-Rt. lateral flexion-Rt. rotation followed by extension-Lt. lateral flexion-Lt. rotation) 3 times weekly for 4 weeks. Outcomes were measured using absolute rotation angle (ARA), anterior weight bearing (AWB), range of flexion and extension motions (RFEM), and neck disability index (NDI) methods before and after the 4-week intervention period. Results: There were significant effects for the PNFG, pre- and post-intervention, in ARA, AWB, RFEM, and NDI. There were significant differences in ARA, AWB, RFEM, and NDI compared with CG. Conclusion: The results of this study suggest the PNF neck pattern could be beneficial for adults with forward head posture.
숲속 공기와 주변 우점수목 정유의 모노테르펜류 분포 비교
오길영 ( Gil Young Oh ),서윤규 ( Yun Gyu Seo ),박귀환 ( Gui Hwan Park ),김익산 ( Ik San Kim ),배주순 ( Ju Soon Bae ),박송인 ( Song In Park ),하훈 ( Hun Ha ),양수인 ( Soo In Yang ),이지헌 ( Ji Hun Lee ),이완진 ( Wan Jin Lee ) 한국산림과학회 2013 한국산림과학회지 Vol.102 No.3
The concentration of terpene (18 species) was measured from spring to fall in ambient air and essential oil at three different forests located in Jeollanamdo province. Sampling was conducted at 4-hour-interval a day of each season. In the ambient air, α-pinene, β-pinene and 2-carene were the most abundant compounds throughout the sampling periods and areas. The highest three-season average concentration of total terpene was 2,597 pptv at Jeamsan(Mt.) in Boseong which was predominantly populated by Pinaceae. The seasonal averages were observed to peak during spring with their max at Palyeongsan(Mt.) in Goheung and Jeamsan(Mt.) in Boseong, and during fall at Woodland in Jangheung. Most of terpenes had diurnal variations with higher concentrations during the daytime, and lower during the nighttime. However in essential oil, the highest annual average concentration of total terpene was 798 μg/dry-g at Goheung which was predominantly populated by Chamaecyparis obtusa. Also, the component proportion ratio showed different distribution between ambient air and essential oil. From the results of this study, we suggest that Chamaecyparis obtusa species is more useful, and younger tree is more economical than older one, and spring is the best season for lumbering in order to use essential oil.
사람의 다형핵 백혈구에서의 Interleukine-1β 유전자 발현에 관한 연구
소서영,송인택,김지혜,유강렬,오희정,김형섭,오귀옥 全北大學校 齒醫學硏究所 1992 전북치대논문집 Vol.10 No.1
Human polymorphonuclear leukocytes(PMN), which share a common cell lineage with macrophage, were tested for the expression of IL-1β and MIP-1α mRNA. PMN were stimulated with rIL-1β, PMA, rMIP-1α, LPS, rIL-2 total cytoplasmic RNA were extracted for the Northern blot analysis. In order to determine the induction kinetics of IL-1β or MIP-1α mRNA expression, cells were stimulated for 0, 1, 2, 3 hours. We found peak expression of IL-1β mRNA after 1hr of induction with rIL-1β, LPS and after 2hr of induction with PMA, rMIP-1α also induced but a scarce IL-1β message front PMN. In contrast to the IL-1β mRNA expression, MIP-1α were not induced from PMN in any culture conditions.