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차용준,박지영,김훈,김소정,정연정,소양순,유영재 창원대학교 생활과학연구소 2000 생활과학연구 Vol.4 No.-
Bacillus cereus KCTC 3674 produced at least two kinds of extracellular proteases. Two major bands of the protease activity with molecular weights of approximately 100 and 38 kDa were obtained after gelatin-SDS-PAGE. The production of extracellular proteases occurred during the logarithmic growth phase and was greatest when cultures reached the stationary growth phase. On the other hand, the growth of B. cereus KCTC 3674 and the production of its extracellular proteases were severely inhibited by the addition of protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP). Thus, it was concluded that the growth of B. cereus KCTC 3674 and the production of its extracellular pretenses were strongly affected by a H+ electrochemical potential.
압궤손상을 입은 혈관에서 Ginkgo Biloba 추출물(EGb 761)이 혈관문합술후 개통율에 미치는 영향
강소라,이동진,차진한,김양우 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3
Replantation after crush amputation has a relatively low success rate. Thrombus formation due to vessel and tissue trauma is considered as the principal cause of failure. In the laboratory and clinically, we have been tried to improve the post-anastomosis patency rate in crushed microvasculature. To accomplish this, we have usually used several anticoagulant drugs. Extracts from the leaves of Ginkgo biloba (EGb) have been used therapeutically for centuries. EGb exerts a number of pharmacologic actions. Eighty rats were control group and another 80 rats were treated with EGb 761. The femoral arteries underwent crush injury with an energy of 0.4J, and the vessles in each group were divided and anastomosed by a standard microsurgical technique. Each group was comprised as follows: ① control group(A1,A2): group A1(n=40); intraluminal saline irrigation, group A2(n=40); intraluminal saline irrigation+heparin 40 u/ml(IV) ② EGb 761 treated group(B1, B2): groupB1(n=40); intraluminal saline irrigation, group B2(n=40); intraluminal saline irrigation + heparin 40 u/ml(IV). At postoperative 14 days, the patency rates were; group A1 20%, group A2 77.5%, group B1 47.5%, group B2 92.5%. These results were interpreted as follows: the patency rate was significantly increased in the EGb 761-only treated group(p<0.01), the heparin-only treated group(p<0.01), and the EGb 761 and heparin-combined at crushed microvessel surgery. However the patency rate of the EGb-only treated group was significantly lower than that of the heparin-only treated group(p<0.01). And in the EGb 761 and heparin-combined treated group compared to the heparin-only treated group, there was some patency rate increase in the combined treated group, but there was no significant difference between them(p=0.060).
<i>Marinoscillum</i> <i>luteum</i> sp. nov., isolated from marine sediment
Cha, In-Tae,Park, Soo-Je,Kim, So-Jeong,Kim, Jong-Geol,Jung, Man-Young,Shin, Kee-Sun,Kwon, Kae Kyoung,Yang, Sung-Hyun,Seo, Yong-Seok,Rhee, Sung-Keun International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.9
<P>A novel strain, designated SJP7<SUP>T</SUP>, was isolated from sediment of the Tofua Arc of the Tonga Trench. The 16S rRNA gene sequence of the isolate showed the highest similarity to that of <I>Marinoscillum pacificum</I> KCCM 42325<SUP>T</SUP> (95.9 %). Phylogenetic analysis revealed that the isolate formed a distinct phyletic line with <I>Marinoscillum pacificum</I> KCCM 42325<SUP>T</SUP> and <I>Marinoscillum furvescens</I> LMG 13023<SUP>T</SUP> within the family <I>Cytophagaceae</I>. Cells of strain SJP7<SUP>T</SUP> were Gram-stain-negative and appeared as long rods that were motile by gliding. Growth was observed at 15–40 °C (optimum, 30 °C), at pH 5.5–9.0 (optimum, pH 7.5–8.0) and in the presence of 0.5–7.0 % (w/v) NaCl (optimum, 2.5–3 %). The major respiratory quinone was MK-7. The dominant fatty acids were summed feature 3 (comprising C<SUB>16 : 1</SUB>ω7<I>c</I> and/or C<SUB>16 : 1</SUB>ω6<I>c</I>), iso-C<SUB>15 : 0</SUB> and C<SUB>16 : 1</SUB>ω5<I>c</I>. The DNA G<I>+</I>C content was 43.5 mol%. These properties support the affiliation of strain SJP7<SUP>T</SUP> with the genus <I>Marinoscillum</I>. Further phenotypic differentiation of strain SJP7<SUP>T</SUP> from other species of the genus <I>Marinoscillum</I> was indicated by the results of physiological and biochemical tests. On the basis of evidence from our polyphasic taxonomic study, strain SJP7<SUP>T</SUP> represents a novel species of the genus <I>Marinoscillum</I>, for which the name <I>Marinoscillum</I> <I>luteum</I> sp. nov. is proposed. The type strain of <I>Marinoscillum</I> <I>luteum</I> is SJP7<SUP>T</SUP> ( = KCTC 23939<SUP>T</SUP> = NCAIM B02491<SUP>T</SUP>).</P>
Effect of the deposition process of window layers on the performance of CIGS solar cells
Cha, Jung-Hwa,Kwon, Soo-Min,Bae, Jin A.,Yang, So Hyun,Jeon, Chan-Wook Elsevier 2017 Journal of alloys and compounds Vol.708 No.-
<P><B>Abstract</B></P> <P>The effects of the formation process of transparent conducting electrodes on the performance of Cu(In,Ga)Se<SUB>2</SUB> thin film solar cells were examined. B:ZnO and (Ga,Al):ZnO thin films deposited using metalorganic chemical vapor deposition (MOCVD) and magnetron sputtering, respectively, were compared in terms of the unit thin films and solar cell device performance. Both unit films formed under optimized process conditions showed similar electrical resistance, whereas the optical reflectance of B:ZnO by MOCVD was superior to that of the sputtered film. In the case of the B:ZnO film deposited on the absorber layer, the surface roughness became greater than the unit film on the test glass. The short-circuit current of the solar cell fabricated with B:ZnO was 3% higher than that with (Ga,Al):ZnO due to the enhanced light scattering. The junction quality and defect chemistry were identical in both devices, which suggests that there was no plasma damage due to sputtering.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sputtered ZnO-based oxide and MOCVD grown oxide showed similar resistivity. </LI> <LI> Both oxide, however, yielded in very different surface morphology. </LI> <LI> Solar cell efficiencies were almost same for both windows. </LI> <LI> Higher voltage from sputtered oxide, and higher current from MOCVD oxide. </LI> <LI> Textured overgrowth of MOCVD oxide was responsible for reduced surface reflection. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( So Yeon Lim ),( Woo Chul Chang ),( Sun Ju Lee ),( Byeong Wook Song ),( Hye Jung Kim ),( Min Ji Cha ),( Sae Yun Kwon ),( Seong Min Han ),( Yang Soo Jang ),( Nam Sik Chung ),( Ki Chul Hwang ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.3
Intracellular calcium ions([Ca2+]i) have an important function in modulating the contractility of all kinds of muscle, including vascular smooth muscle cells. Reactive oxygen species(ROS) can induce Ca2+-overload by directly affecting the Ca2+ handling proteins. Little is known that phospholipase C(PLC) isozymes are involved in the Ca2+-overload induced by ROS in smooth muscle cells. [Ca2+]i response to exogenous H2O2 or ionopore A23187 was measured using the fluorescent probe, fura2-AM, in rat aortic smooth muscle cells(RASMCs). The differential expression levels of PLC isozymes were measured in cells exposed actually with H2O2 or ionopore. The subsequent signaling cascades related with PLC isozymes were also investigated in RASMCs. H2O2(1 mM) inhibited the proliferation of RASMCs in DMEM containing 0.1% FBS and increased the [Ca2+]i by a 50%. In H2O2-stimulated RASMCs, the expression of PLC-δ1 was suppressed within 1 hr, but the other PLC isozymes, ß-, and γ-form were not affected. In contrast, the expression of PLC-δ1 and PLC-γ1 were suppressed by ionophore, A23187 (10 mM), for 3 hrs. The activity of protein kinase C(PKC) was also suppressed in same trend of PLC-δ1, not PLC-γ1. In conclusion, the H2O2-induced Ca2+ overload may differentially suppress the expression of PLC isozymes in RASMCs and the decreased expression of PLC-δ1 subsequently inhibited the PKC activity. These results suggest that the PLC/ PKC-regulated Ca2+homeostasis is the crucial targets of exogenous ROS in H2O2-stimulated RASMC.
Yang, Joo Yun,Cha, Jihei,Shim, So-Yeon,Cho, Su Jin,Park, Eun Ae The Korean Pediatric Society 2014 Clinical and Experimental Pediatrics (CEP) Vol.57 No.4
Purpose: Eosinophilia is common in premature infants, and its incidence increases with a shorter gestation period. We investigated the clinical significance of eosinophilia in premature infants born at <34 weeks gestation. Methods: We analyzed the medical records of premature infants born at <34 weeks gestation who were admitted to the neonatal intensive care unit at Ewha Womans University Mokdong Hospital between January 2003 and September 2010. Eosinophilia was defined as an eosinophil percentage of >3% of the total leukocytes. Perinatal parameters and clinical parameters were also analyzed. Results: Of the 261 infants born at <34 weeks gestation, 22.4% demonstrated eosinophilia at birth. The eosinophil percentage peaked in the fourth postnatal week at 7.5%. The incidence of severe eosinophilia increased after birth up to the fourth postnatal week when 8.8% of all patients had severe eosinophilia. Severity of eosinophilia was positively correlated with a lower gestational age, birth weight, and Apgar score. Respiratory distress syndrome, bronchopulmonary dysplasia, nephrocalcinosis, intraventricular hemorrhage, and sepsis were associated with a higher eosinophil percentage. The eosinophil percentage was significantly higher in infants with bronchopulmonary dysplasia from the first postnatal week and the percentage was the highest in the fourth postnatal week, with the maximal difference being 4.1% (P<0.001). Conclusion: Eosinophilia is common in premature infants and reaches peak incidence and severity in the fourth postnatal week. The eosinophil percentage was significantly higher in bronchopulmonary dysplasia patients from the first postnatal week. Severe eosinophilia was significantly associated with the incidence of bronchopulmonary dysplasia even after adjusting for other variables.