Stem-leaf saponins from Panax notoginseng counteract aberrant autophagy and apoptosis in hippocampal neurons of mice with cognitive impairment induced by sleep deprivation

Cao, Yin,Yang, Yingbo,Wu, Hui,Lu, Yi,Wu, Shuang,Liu, Lulu,Wang, Changhong,Huang, Fei,Shi, Hailian,Zhang, Beibei,Wu, Xiaojun,Wang, Zhengtao The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.3

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioning at molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegeneration in the central nervous system. This study is aimed to explore the alleviative effect and the underlying molecular mechanism of stem-leaf saponins of Panax notoginseng (SLSP) on the abnormal neuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory induced by SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronal morphological changes were observed by Nissl staining. Autophagosome formation was examined by transmission electron microscopy, immunofluorescent staining, acridine orange staining, and transient transfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin V staining. The expression or activation of autophagy and apoptosis-related proteins were detected by Western blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h, accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampal neurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycin and re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transduction in HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probably through suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings may contribute to the clinical application of SLSP in the prevention or therapy of neurological disorders associated with SD.

Stem-leaf saponins from Panax notoginseng counteract aberrant autophagy and apoptosis in hippocampal neurons of mice with cognitive impairment induced by sleep deprivation

Yin Cao,Yingbo Yang,Hui Wu,Yi Lu,Shuang Wu,Lulu Liu,Changhong Wang,Fei Huang,Hailian Shi,Beibei Zhang,Xiaojun Wu,Zhengtao Wang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioningat molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegenerationin the central nervous system. This study is aimed to explore the alleviative effect and theunderlying molecular mechanism of stemeleaf saponins of Panax notoginseng (SLSP) on the abnormalneuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory inducedby SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronalmorphological changes were observed by Nissl staining. Autophagosome formation was examined bytransmission electron microscopy, immunofluorescent staining, acridine orange staining, and transienttransfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin Vstaining. The expression or activation of autophagy and apoptosis-related proteins were detected byWestern blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h,accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampalneurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycinand re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transductionin HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probablythrough suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings maycontribute to the clinical application of SLSP in the prevention or therapy of neurological disordersassociated with SD.

Transcriptome profiling analysis reveals the role of Elongases in development and reproduction of Tribolium castaneum

Xue Shuang,Gao Shanshan,Cao Yizhuo,Gao Tian,Yin Se,Zhang Yong-lei,Lu Ruixue,Zhang Kunpeng 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Elongases (ELO) is a large class of enzymes involved in the development and reproduction of insects. Although some ELOs have been isolated and characterized in a small number of insects, little is known about the specific function of ELOs in insects, especially in the regulatory mechanism of ELOs. In this study, the function and regulation of TcELO were investigated in Tribolium castaneum. Spatiotemporal expression profiling indicated that TcELO expressed in all development stages, and high expressed in the head, epidermis, and ovary of T. castaneum. By comparing the transcriptome profiles of RNAi-treated larvae (dsTcELO) and control larvae of T. canstaneum using RNA sequencing, 320 differentially expressed genes (DEGs) were identified from the ds-ELO-treated group. Compared with the control group, five genes including cytc2, sfxn3 and H2A.V were significantly up-regulated in the dsTcELO group, suggesting that TcELO may be involved in the apoptosis of insect biological cells to control the growth and development system of insects. However, NAT was significantly down-regulated, indicating that it cooperates with TcELO to control the reproductive process of insects. Knockdown of TcELO also significantly up-regulated related genes in environmental adaptation pathways, such as COXIV, COXc6, and ATPs, which suggested that these genes may regulate TcELO to involve in epidermal function of insects and thier adaptability to the environment. These results provide new insights into the regulatory mechanism of TcELO involved in insect physiology, and can provide theoretical support for the control of agricultural pests.

Analysis on mechanical behavior of dovetail mortise-tenon joints with looseness in traditional timber buildings

Yizhu Li,Shuang-Yin Cao,Jian-yang Xue 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.60 No.5

To study the effect of looseness on mechanical behavior of dovetail mortise-tenon joints, five dovetail mortise-tenon joints, including one intact joint and four loose joints, were fabricated and tested under cycle lateral loadings, and non-linear finite element models using the software ABAQUS were also developed. The effects of looseness on stress distribution, rotational stiffness and bearing capacity of joints were studied based on the analysis of test and simulation results. The results indicate that the hysteretic loops are anti-Z-shaped and present typical characteristics of pinching and slippage, the envelop curves of joints are classified as following two stages: elastic and strengthening stage. The peak stress, rotational stiffness and bearing capacity of joints were reduced due to looseness. The moment-rotation theoretical model of intact joint was simplified in terms of the relation of construction dimensions for buildings, and the moment-rotation theoretical model considering the effect of looseness was proposed and validated.

New technique for strengthening reinforced concrete beams with composite bonding steel plates

Su-hang Yang,Shuang-Yin Cao,Rui-nan Gu 국제구조공학회 2015 Steel and Composite Structures, An International J Vol.19 No.3

Composite bonding steel plate (CBSP) is a newly developed type of structure strengthened technique applicable to the existing RC beam. This composite structure is applicable to strengthening the existing beam bearing high load. The strengthened beam consists of two layers of epoxy bonding prestressed steel plates and the RC beam sandwiched in between. The bonding enclosed and prestressed U-shaped steel jackets are applied at the beam sides. This technique is adopted in case of structures with high longitudinal reinforcing bar ratio and impracticable unloading. The prestress can be generated on the strengthening steel plates and jackets by using the CBSP technique before loading. The test results of full-scale CBSP strengthened beams show that the strength and stiffness are enhanced without reduction of their ductility. It is demonstrated that the strain hysteresis effect can be effectively overcome after prestressing on the steel plates by using such technique. The applied plates and jackets can jointly behave together with the existing beam under the action of epoxy bonding and the mechanical anchorage of the steel jackets. The simplified formulas are proposed to calculate the prestress and the ultimate capacities of strengthened beams. The accuracy of formulas was verified with the experimental results.

Flexural behaviour of steel plate-masonry composite beams

Deng-Hu Jing,Shuang-Yin Cao,Lei Shi 국제구조공학회 2012 Steel and Composite Structures, An International J Vol.13 No.2

Steel plate-masonry composite structure is a newly-developed type of structural technique applicable to existing masonry buildings by which the load-bearing walls can be removed for large spaces. This kind of structure has been used in practice for its several advantages, but experimental investigation on its elements is nearly unavailable in existing literature. This paper presents an experimental study on the flexural behaviour of four steel plate-masonry composite beams loaded by four-point bending. Test results indicate that failure of the tested beams always starts from the local buckling of steel plate, and that the tested beams can satisfy the requirement of service limit state. In addition, the assumption of plane section is still remained for steel plate prior to local buckling or steel yielding. By comparative analyses, it was also verified that the working performance of the beam is influenced by the cross-section of steel plate, which can be efficiently enhanced by epoxy adhesive rather than cement mortar or nothing at all. Besides, it was also found that the contribution of the encased masonry to the flexural capacity of the composite beam cannot be ignored when the beam is injected with epoxy adhesive.

Fatigue behavior of concrete beams reinforced with HRBF500 steel bars

Li, Ke,Wang, Xin-Ling,Cao, Shuang-Yin,Chen, Qing-Ping Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.2

The purpose of this study was to investigate experimentally the fatigue performance of reinforced concrete (RC) beams with hot-rolled ribbed fine-grained steel bars of yielding strength 500MPa (HRBF500). Three rectangular and three T-section RC beams with HRBF500 bars were constructed and tested under static and constant-amplitude cyclic loading. Prior to the application of repeated loading, all beams were initially cracked under static loading. The major test variables were the steel ratio, cross-sectional shape and stress range. The stress evolution of HRBF500 bars, the information about crack growth and the deflection developments of test beams were presented and analyzed. Rapid increases in deflections and tension steel stress occured in the early stages of fatigue loading, and were followed by a relatively stable period. Test results indicate that, the concrete beams reinforced with appropriate amount of HRBF500 bars can survive 2.5 million cycles of constant-amplitude cyclic loading with no apparent signs of damage, on condition that the initial extreme tensile stress in HRBF500 steel bars was controlled less than 150 MPa. It was also found that, the initial extreme tension steel stress, stress range, and steel ratio were the main factors that affected the fatigue properties of RC beams with HRBF500 bars, whose effects on fatigue properties were fully discussed in this paper, while the cross-sectional shape had no significant influence in fatigue properties. The results provide important guidance for the fatigue design of concrete beams reinforced with HRBF500 steel bars.

Fatigue behavior of concrete beams reinforced with HRBF500 steel bars

Ke Li,Xin-Ling Wang,Shuang-Yin Cao,Qing-Ping Chen 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.2

The purpose of this study was to investigate experimentally the fatigue performance of reinforced concrete (RC) beams with hot-rolled ribbed fine-grained steel bars of yielding strength 500MPa (HRBF500). Three rectangular and three T-section RC beams with HRBF500 bars were constructed and tested under static and constant-amplitude cyclic loading. Prior to the application of repeated loading, all beams were initially cracked under static loading. The major test variables were the steel ratio, crosssectionalshape and stress range. The stress evolution of HRBF500 bars, the information about crack growth and the deflection developments of test beams were presented and analyzed. Rapid increases in deflections and tension steel stress occured in the early stages of fatigue loading, and were followed by a relatively stable period. Test results indicate that, the concrete beams reinforced with appropriate amount of HRBF500 bars can survive 2.5 million cycles of constant-amplitude cyclic loading with no apparent signs of damage, on condition that the initial extreme tensile stress in HRBF500 steel bars was controlled less than 150 MPa. It was also found that, the initial extreme tension steel stress, stress range, and steel ratio were the main factors that affected the fatigue properties of RC beams with HRBF500 bars, whose effects on fatigueproperties were fully discussed in this paper, while the cross-sectional shape had no significant influence in fatigue properties. The results provide important guidance for the fatigue design of concrete beams reinforced with HRBF500 steel bars.

Experimental investigation of masonry walls supported by steel plate-masonry composite beams

Deng-Hu Jing,Jian-Fei Chen,Giuseppina Amato,Ting Wu,Shuang-Yin Cao 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.6

Masonry walls are sometimes removed in buildings to either make new passages or increase the usable space. This may change the loading paths in the structure, and require new beams to transfer the loads which are carried by the masonry walls that are to be removed. One possible method of creating such new beams is to attach steel plates onto part of the existing walls to form a steel plate-masonry composite (SPMC) beam, leading to a new structure with part of the masonry wall supported by a new SPMC beam. This paper presents an experimental investigation into the interaction between the SPMC beam and the masonry wall above. Five SPMC beams supporting a masonry wall were tested to study the influence of parameters including the height-to-span ratio of the masonry wall, height of the beam and thickness of the steel plates. The test results, including failure mode, load-carrying capacity, load-deflection curves and strain distribution, are presented and discussed. It is found that for developing better arching effect in the masonry wall the ratio of the in-plane flexural stiffness of the masonry wall to the flexural stiffness of the SPMC beam must be between 2.8 and 7.1.

Stereo-digital image correlation in the behavior investigation of CFRP-steel composite members

Xiao-Yuan He,Yun-Tong Dai,Hai-Tao Wang,Tian-Yuan Ge,Gang Wu,Jian-Xiao Wan,Shuang-Yin Cao,Fu-Jun Yang 국제구조공학회 2017 Steel and Composite Structures, An International J Vol.23 No.6

The application of carbon fiber reinforced polymer (CFRP) in steel structures primarily includes two categories, i.e., the bond-critical application and the contact-critical application. Debonding failure and buckling failure are the main failure modes for these two applications. Conventional electrometric techniques may not provide precise results because of the limitations associated with single-point contact measurements. A nondestructive full-field measurement technique is a valuable alternative to conventional methods. In this study, the digital image correlation (DIC) technique was adopted to investigate the bond behavior and buckling behavior of CFRP-steel composite members. The CFRP-to-steel bonded joint and the CFRPstrengthened square hollow section (SHS) steel column were tested to verify the suitability of the DIC technique. The stereo- DIC technique was utilized to measure continuous deformation. The bond-slip relationship of the CFRP-to-steel interface was derived using the DIC data. Additionally, a multi-camera DIC system consisting of four stereo-DIC subsystems was proposed and applied to the compressive test of CFRP-strengthened SHS steel column. The precise buckling location and CFRP delamination of the CFRP-strengthened SHS steel column were identified. The experimental results confirm that the stereo-DIC technique can provide effective measurements for investigating the behaviors of CFRP-steel composite members.