Liposome-mediated Induction of Apoptosis of Human Hepatoma Cells by C-Myc Antisense Phosphorothioate Oligodeoxynucleotide and 5-Fluorouracil

Yuan, Yuan,Cai, Hui,Yang, Xiao-Jun,Li, Wei,He, Jin,Guo, Tian-Kang,Chen, Yi-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: The aim of this study was to investigate the effect of a c-myc antisense oligodeoxynucleotide and 5-fluorouracil on the expression of c-myc, invasion and proliferation of HEPG-2 liver cancer cells. Materials and Methods: HEPG-2 cells were treated with lipiosome-mediated c-myc ADSON and 5-fluorouracil. The proliferation inhibition rate and invasion were measured by MTT and invasion assay, respectively. Cell apoptosis was detected by flow cytometry and expression of c-myc by RT-PCR and immunohistochemistry. Results: The proliferation inhibition rate was significantly higher in the antisense oligodeoxynucleotide added-5-fluorouracil group than single antisense oligodeoxynucleotide or 5-fluorouracil group (p<0.05). G0/G1 cells in the antisense oligodeoxynucleotide group and S cells in the 5-fluorouracil groups were significantly increased than that in the control group, respectively (P<0.01). The amplification strips of PCR products in 5-FU, ASODN and combination groups were significantly weaker than that in the control group (P<0.01). The percentage of c-myc-protein-positive cells were significantly lower in antisense oligodeoxynucleotide, 5-fluorouracil and combination groups than that in the control group (P<0.01). Conclusions: A liposome-mediated c-myc antisense oligodeoxynucleotide and 5-fluorouracil can inhibit the proliferation and invasion of liver cancer cells by reducing the expression of c-myc. A c-myc antisense oligodeoxynucleotide can increase the sensitivity of liver cancer cells to 5-fluorouracil and decrease the dosage of the agent necessary for efficacy, providing an experimental basis for the clinical therapy of liver cancer.

Stereo-digital image correlation in the behavior investigation of CFRP-steel composite members

Xiao-Yuan He,Yun-Tong Dai,Hai-Tao Wang,Tian-Yuan Ge,Gang Wu,Jian-Xiao Wan,Shuang-Yin Cao,Fu-Jun Yang 국제구조공학회 2017 Steel and Composite Structures, An International J Vol.23 No.6

The application of carbon fiber reinforced polymer (CFRP) in steel structures primarily includes two categories, i.e., the bond-critical application and the contact-critical application. Debonding failure and buckling failure are the main failure modes for these two applications. Conventional electrometric techniques may not provide precise results because of the limitations associated with single-point contact measurements. A nondestructive full-field measurement technique is a valuable alternative to conventional methods. In this study, the digital image correlation (DIC) technique was adopted to investigate the bond behavior and buckling behavior of CFRP-steel composite members. The CFRP-to-steel bonded joint and the CFRPstrengthened square hollow section (SHS) steel column were tested to verify the suitability of the DIC technique. The stereo- DIC technique was utilized to measure continuous deformation. The bond-slip relationship of the CFRP-to-steel interface was derived using the DIC data. Additionally, a multi-camera DIC system consisting of four stereo-DIC subsystems was proposed and applied to the compressive test of CFRP-strengthened SHS steel column. The precise buckling location and CFRP delamination of the CFRP-strengthened SHS steel column were identified. The experimental results confirm that the stereo-DIC technique can provide effective measurements for investigating the behaviors of CFRP-steel composite members.

Mechanism of the anti-liver fibrosis effect of Periplaneta americana extracts that promote apoptosis of HSC-T6 cells through the Bcl-2/Bax signaling pathway

Yuan Liping,Yang Xiao,He Ying,Zhao Yanwen,Chen Yi,Yang Yongshou,Xiao Peiyun 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Periplaneta americana L. (Blattidae) extract (PA-B) possesses anti-oxidant, anti-inflammatory, and hep atoprotective properties; however, its mechanism of action in liver fibrosis remains unclear. Herein, we inves tigated the hepatoprotective effect and mechanism of action of PA-B. HSC-T6 cells treated with PA-B were stimulated with TGF-β1, and immunofluorescence, western blotting, Hoechst 33,342 staining, confocal laser scanning microscopy, and flow cytometry were performed. Furthermore, the effects of PA-B were verified in a rat model of hepatic fibrosis established using 40 % carbon tetrachloride (CCl 4 ). PA-B induced apoptosis of HSC-T6 cells by inhibiting proliferation (maximum inhibition ratio 99.08 %), reducing mitochondrial membrane po tential, and ameliorating apoptosis-related proteins (P < 0.01). PA-B (120 mg/kg) significantly alleviated CCl 4 -induced hepatic histopathological fibrosis and reduced the levels of aspartate aminotransferase (from 27.68 to 20.58 U/L) and alanine aminotransferase (from 16.88 to 5.93 U/L; P < 0.05 or P < 0.01). Immunohistochemistry and western blotting showed that α-SMA levels were reduced (from 0.49 to 0.23) and that the Bcl/Bax signaling pathway was significantly inhibited by PA-B (120 mg/kg; P < 0.05 or P < 0.01). PA-B exhibits anti-fibrotic effects by mediating the Bcl-2/Bax pathway and promoting HSC-T6 cell apoptosis, providing a basis for its development as a drug to treat liver fibrosis.

Research on Thymopentin Loaded Oral N-Trimethyl Chitosan Nanoparticles

Xiao-jia Yuan,Zhi-rong Zhang,Qing-guo Song,Qin He 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.9

Peptides, although high efficacy and specificity in their physiological function, usually have low therapeutical activities due to their poor bioavailability when administrated orally. Nanoparticles have been regarded as a useful vector for targeted drug delivery system because they can protect drug from being degraded quickly and pass the gastrointestinal barriers. Here we described a novel oral N-trimethyl chitosan nanoparticles formulation containing thymopentin (Tp5-TMC-NP). N-trimethyl chitosan (TMC) was synthesized and then used to prepare Tp5- TMC-NP by ionotropic gelation. A three-factor, five-level CCD (Central Composite Design) design was used in the optimization procedure, with HPLC as the analyzing method. The resulting Tp5-TMC-NP had a regular spherical surface and a narrow particle size range with a mean diameter of 110.6 nm. The average entrapment efficiency was 78.8%. The lyophilized Tp5-TMC-NP formulation was stable in 4oC or -20oC after storage of 3 months without obvious changes in morphology, particle size, pH and entrapment ratio. The results of the flow cytometer determination showed that the ratio of CD4+/CD8+ of Wistar female rat givenTp5-TMC-NP (ig) was 2.59 time that of the group given Tp5 (ig).

Study on micro-scale 3D numerical modeling and droplet deposition of plain weave fabric

Yuan Xiao,Chengkun Zhang,Qian LI,Pengcheng Yang,Dan He 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.4

To define the deposition and morphology changes of the droplets on fabric medium clearly in spraying printing the electrical circuits on fabric surface. The Navier-Stokes (NS) equation and volume of fluid (VOF) boundary tracking model are adopted to set up a microscale three-dimensional (3D) numerical model for plain weave fabric. Simulation focused on the evolution process of phase field, pressure field and speed field during the droplets deposition. The results show that axial momentum controls the droplets outward spread quickly and synchronously with a ring shape at early stage. The control of unbalanced-Young driving force causes the three-phase contact line fixes on fiber cylinder, droplets are in an instantaneous stable state. Capillary pressure gradient controls the three-phase contact line pinning and slippage forward lead to differences in spreading direction. The rebound effect of droplets promotes further penetration of droplets into fabric. Experiment analysis displays the well match with the simulation results, indicating the rationality of the proposed micro-scale 3D plain weave fabric modeling method. The findings of this paper would contribute to the future works for flexible electronic devices on fabric surface research studies.

Research on Thymopentin Loaded Oral N-Trimethyl Chitosan Nanoparticles

Yuan, Xiao-Jia,Zhang, Zhi-Rong,Song, Qing-Guo,He, Qin The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.9

Peptides, although high efficacy and specificity in their physiological function, usually have low therapeutical activities due to their poor bioavailability when administrated orally. Nanoparticles have been regarded as a useful vector for targeted drug delivery system because they can protect drug from being degraded quickly and pass the gastrointestinal barriers. Here we described a novel oral N-trimethyl chitosan nanoparticles formulation containing thymopentin (Tp5-TMC-NP). N-trimethyl chitosan (TMC) was synthesized and then used to prepare Tp5-TMC-NP by ionotropic gelation. A three-factor, five-level CCD (Central Composite Design) design was used in the optimization procedure, with HPLC as the analyzing method. The resulting Tp5-TMC-NP had a regular spherical surface and a narrow particle size range with a mean diameter of 110.6 nm. The average entrapment efficiency was 78.8%. The lyophilized Tp5-TMC-NP formulation was stable in $4^{\circ}C\;or\;-20^{\circ}C$ after storage of 3 months without obvious changes in morphology, particle size, pH and entrapment ratio. The results of the flow cytometer determination showed that the ratio of $CD4^+/CD8^+$ of Wistar female rat given Tp5-TMC-NP (ig) was 2.59 time that of the group given Tp5 (ig).

Vibrio alginolyticus MviN is a LuxO-regulated Protein and Affects Cytotoxicity Towards Epithelioma Papulosum Cyprini(EPC)Cells

( Xiao Dan Cao ),( Qi Yao Wang ),( Qin Liu ),( Huan Liu ),( Hong Hong He ),( Yuan Xing Zhang ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.2

Utilization of cane molasses for docosahexaenoic acid production by Schizochytrium sp. CCTCC M209059

He Huang,Lu-Jing Ren,Juan Li,Yuan-Wei Hu,Xiao-Jun Ji 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.4

Cane molasses (CM), an agro-industrial by-product, was first examined for docosahexaenoic acid (DHA)production by Schizochytrium sp. Cell dry weight as 21.94 g/L at treated CM cultivation was similar to that at pure glucose (26.7 g/L) cultivation. Batch fermentation at different initial CM concentration showed that DHA percentage could reach 47.51% at 10 g/L CM but only 37.90% at 70 g/L CM. By analyzing the fermentation process, monosodium glutamate might be a positive agent for effective DHA production. Finally, monosodium glutamate and malic acid were introduced to the fed-batch fermentation for effective DHA production.

Voice Activity Detection Algorithm based on Improved Radial Basis Function Neural Network

Bao-yuan Chen,Ya-qiong Lan,Jing-yang Liu,Zi-he Li,Xiao-yang Yu 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

Voice activity detection (VAD) is the key of voice recognition, voice synthesis and speech-sound enhancement.For the sake of improve the accuracy and robustness of speech endpoint detection system. Combining the advantages of adaptive genetic algorithm (AGA) and improved radial basis function network (RBF) defects in existing learning methods. This paper presents a comprehensive detection method-- Adaptive genetic algorithm radial basis function network. This method uses adaptive genetic algorithm to simultaneously optimize the center, the width and the structure of RBF network. The method using wavelet analysis to extract the characteristics of the speech signal, use them as an input amount to the radial basis function networks. Establish voice detection system model, this method enhance the accuracy of the detection system and has better robustness.

American ginseng attenuates azoxymethane/dextran sodium sulfate-induced colon carcinogenesis in mice

Chunhao Yu,Xiao-Dong Wen,Zhiyu Zhang,Chun-Feng Zhang,Xiao-Hui Wu,Adiba Martin,Wei Du,Tong-Chuan He,Chong-Zhi Wang,Chun-Su Yuan 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3

Background: Colorectal cancer is a leading cause of cancer-related death, and inflammatory bowel disease is a risk factor for this malignancy. We previously reported colon cancer chemoprevention potential using American ginseng (AG) in a xenograft mice model. However, the nude mouse model is not a gutspecific colon carcinogenesis animal model. Methods: In this study, an experimental colitis and colitis-associated colorectal carcinogenesis mouse model, chemically induced by azoxymethane/dextran sodium sulfate (DSS) was established and the effects of oral AG were evaluated. The contents of representative ginseng saponins in the extract were determined. Results: AG significantly reduced experimental colitis measured by the disease activity index scores. This suppression of the experimental colitis was not only evident during DSS treatment, but also very obvious after the cessation of DSS, suggesting that the ginseng significantly promoted recovery from the colitis. Consistent with the anti-inflammation data, we showed that ginseng very significantly attenuated azoxymethane/DSS-induced colon carcinogenesis by reducing the colon tumor number and tumor load. The ginseng also effectively suppressed DSS-induced proinflammatory cytokines activation using an enzyme-linked immunosorbent assay array, in which 12 proinflammatory cytokine levels were assessed, and this effect was supported subsequently by real-time polymerase chain reaction data. Conclusion: AG, as a candidate of botanical-based colon cancer chemoprevention, should be further investigated for its potential clinical utility.