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Co-culture platform for neuron-astrocyte interaction using optogenetic modulation
Seoyoung Hwang 대한의용생체공학회 2022 Biomedical Engineering Letters (BMEL) Vol.12 No.4
For decades, the role of glial cells has attracted attention in the neuroscience field. Particularly, although the astrocyte is themost abundant glial cell type, it was believed to function as a passive support cell. However, recent evidence suggests thatastrocytes actively release various gliotransmitters and signaling entities that regulate the excitability of pre-and post-synapticneurons in the brain. In this study, we optimized the ratio of astrocytes and neurons to investigate the interaction betweenastrocytes and neurons. To this end, postnatal day 0–1 rodent hippocampi were dissociated and cultured. The neuron–astrocyteratio was monitored for up to 3 weeks after treating the cultures with 0, 1, and 5 μM of cytosine arabinoside (Ara-C)at DIV 2. Subsequently, from postnatal transgenic (TG) mouse expressing ChR2 on astrocytes, hippocampi were culturedon the microelectrode array (MEA) with the desired neuron–astrocyte ratio. The astrocyte was irradiated using a 473 nmblue laser for 30 s in a cycle of 10 Hz and electrophysiological recording was performed to verify the activities of neuronsinduced by the stimulated astrocytes. Astrocytes and neurons in both co-cultures increased at an identical ratio when treatedwith 1 μM Ara-C, whereas they decreased significantly when treated with 5 μM Ara-C. Particularly, the laser-stimulatedastrocytes induced an increase in the frequency of neuronal activities and lasted after illumination. The proposed co-cultureplatform is expected to be used in experiments to investigate the network between astrocytes and neurons in vitro.
도시농업 확산을 위한 가정용 수경재배기의 소비자 구매의도 결정요인 분석
황서영(Hwang, Seoyoung),유지영(Yu, Jiyeong),문정훈(Moon, Junghoon),박병준(Park, Byungjoon) 한국농식품정책학회 2016 농업경영정책연구 Vol.43 No.4
For driving of urban agriculture, Korean Agricultural Research Services invented and distrubuted the household hydroponic instrument in 2014. This paper aims to empirically examine how the characteristics of household hydroponic instrument affect consumer’s purchase intention. This research determined the usability factors and causes of using household hydroponic instrument through the interview of experts on hydroponics. In this study, we emply exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) to suggest the usability factors of household hydroponic instrument use and to find the relationship between the usability factors and consumer’s purchase intention. The results indicate that the educatory purpose of using household hydroponic instrument is the highest factor in this research. Additional practical and political implications of these findings are also discussed in the paper.
First Report of Brain Abscess Associated with Pseudozyma species in a Patient with Astrocytoma.
Hwang, Sangsun,Kim, Juwon,Yoon, Seoyoung,Cha, Yeji,Kim, Myungsook,Yong, Dongeun,Chang, Jong Hee,Jeong, Seok Hoon,Uh, Young,Lee, Kyungwon 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.3
<P>A yeast-like strain was isolated from the brain abscess of a patient diagnosed with astrocytoma. Morphological and molecular analysis on D1/D2 domain in the 26S rRNA gene and internal transcript spacer region of the strain revealed that the strain belonged to the genus Pseudozyma. To the best of our knowledge, this is the first report on the isolation of a Pseudozyma strain from brain abscess.</P>
Hwang, In Hyun,Oh, Joonseok,Zhou, Wei,Park, Seoyoung,Kim, Joo-Hyun,Chittiboyina, Amar G.,Ferreira, Daneel,Song, Gyu Yong,Oh, Sangtaek,Na, MinKyun,Hamann, Mark T. American Chemical Society and American Society of 2015 Journal of natural products Vol.78 No.3
<P/><P>Colorectal cancer has emerged as a major cause of death in Western countries. Down-regulation of β-catenin expression has been considered a promising approach for cytotoxic drug formulation. Eight 4,9-friedodrimane-type sesquiterpenoids (<B>1</B>–<B>8</B>) were acquired using the oxidative potential of <I>Verongula rigida</I> on bioactive metabolites from two <I>Smenospongia</I> sponges. Compounds <B>3</B> and <B>4</B> contain a 2,2-dimethylbenzo[<I>d</I>]oxazol-6(2<I>H</I>)-one moiety as their substituted heterocyclic residues, which is unprecedented in such types of meroterpenoids. Gauge-invariant atomic orbital NMR chemical shift calculations were employed to investigate stereochemical details with support of the application of advanced statistics such as CP3 and DP4. Compounds <B>2</B> and <B>8</B> and the mixture of <B>3</B> and <B>4</B> suppressed β-catenin response transcription (CRT) via degrading β-catenin and exhibited cytotoxic activity on colon cancer cells, implying that their anti-CRT potential is, at least in part, one of their underlying antineoplastic mechanisms.</P>
Hwang, Sinwoo,Baek, Seoyoung,Choi, Yongbaek 한국간호과학회 2021 한국간호과학회 학술대회 Vol.2021 No.10
Aim(s): In the current Army health care delivery system, soldiers have received primary health care from the medical company. If the symptoms get worse, they are transferred to the medical battalion or military hospital. However, the medical company has a problem in providing timely care because of the lack of medical personnel and equipment, which worsens patient conditions and reduces patient satisfaction. Therefore, we conducted a pilot project that the medical company focuses on the role of evacuation and the medical battalion reinforces functions of primary health. The purpose of this study was to compare patient satisfaction in health care before and after the pilot project. Method(s): This study was a nonequivalent control group pretest-posttest design, recruiting 82 soldiers who experienced in medical services before and after pilot projects. The patient satisfaction was investigated as in 4 criteria: accessibility, system infrastructure, care coordination, and patient-centeredness. The pilot project was conducted from September 2019 to April 2020, and the data were from January 2019 to March 2020. Result(s): The results of independent t-test showed that the soldiers evaluated the system infrastructure sector was significantly increased (p=.003) while the accessibility was decreased (p=<0.001) after the pilot project compared to before the pilot project. The care coordination and the patient-centeredness were increased as well after the pilot project, but their figures were not statistically significant. Conclusion(s): This study identifies that the pilot project on improvement of medical battalion"s function has a positive impact on patient satisfaction. In addition, it suggests that strengthening strategies on accessibility should be prioritized in order to expand the pilot project.
Baek, Seoyoung,Hwang, Sinwoo,Choi, Yongbaek 한국간호과학회 2021 한국간호과학회 학술대회 Vol.2021 No.10
Aim(s): Soldiers have received primary health care from the medical company in Republic of Korea Army, and they are transferred to the medical battalion or military hospital if needed. However, the medical company has a restriction in providing timely care because of the lack of resources, which causes a decrease quality of primary health care. Therefore, we conducted a pilot project that the medical battalion conducted unctions of primary health care instead of the medical company. The purpose of the study was to compare performance indicators of the primary health care before and after the pilot projects. Method(s): This study evaluated differences in performance indicators of primary health care before and after the pilot projects. The indicators consisted of three categories: accessibility (travel time to medical unit, waiting time per patient, and the number of untreated among outpatient visits), system infrastructure (the number of X-ray inspections and pathological tests), and care coordination (the number of transferring to an advanced or private hospitals). The pilot project was conducted from September 2019 to April 2020, and the data were collected from January 2019 to March 2020. Result(s): The system infrastructure and care coordination were improved while accessibility was deteriorated. Travel time to medical unit, waiting time, and the number of untreated patients were increased. The number of X-ray inspections and pathological tests were increased by 23.0% and 20.2%, respectively. The number of transferring was increased by 29.9% as well. Conclusion(s): This study identifies that the pilot project on improvement of medical battalion"s function was effective in improving system infrastructure and care coordination. Meanwhile, this study may suggest that strengthening strategies for accessibility should be prioritized to expand the pilot projects.
Kim, Wonyoung,Hwang, Sungmin,Kim, Tae Young,Ham, Won Gyu,Kim, Seoyoung,Lee, Minbaek,Hwangbo, Chang Kwon American Scientific Publishers 2018 Science Of Advanced Materials Vol.10 No.5
<P>A facile route to the fabrication of a broadband Al wire grid polarizer (WGP) was demonstrated using nanoimprint lithography (NIL) and oblique angle deposition (OAD) methods. An Al film with the desired shape for a 1D grating pattern was formed by OAD directly on the top of a polymer nanostructure, which was patterned by NIL as a substrate at a pitch of 120 nm, width of 50 nm and height of 160 nm. The results show that transmittance of the transverse magnetic wave for the fabricated WGP was an average of 67% and the average polarization extinction ratio was 22 dB in the visible and near infrared wavelength regime. The optical properties of the various shapes of Al patterns on the 1D polymer nanostructures were also simulated using a rigorous finite difference time domain simulation. This work can open up a cost-effective fabrication method for high- performance and large-area WGPs.</P>