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Crystallization and preliminary X-ray analysis of API5-FGF2 complex
Seoung Min Bong,Byung Il Lee 한국구조생물학회 2018 Biodesign Vol.6 No.4
API5 is a unique oncogenic, non-BIR type IAP nuclear protein and is up-regulated in several cancers. It exerts several functions, such as apoptosis inhibition, cell cycle progression, cancer immune escape, and anticancer drug resistance. Although structural studies of API have revealed that API5 mediates protein-protein interactions, its detailed molecular functions remain unknown. Since FGF2 is one of API5’s major interacting proteins, structural studies of the API5-FGF2 complex will provide insight into both proteins’ molecular function. We overexpressed and purified API5 and FGF2 in Escherichia coli and crystallized the API-FGF2 complex using polyethylene glycol (PEG) 6000 as a precipitant. Diffraction data were collected to a 2.7 Å resolution using synchrotron X-rays. Preliminary diffraction analysis revealed that the API5-FGF2 complex crystal belongs to the space group P2₁2₁2₁ with the following unit cell parameters: a = 46.862, b = 76.523, c = 208.161 Å. One asymmetric unit with 49.9% solvent contains one API5-FGF2 complex. Molecular replacement calculation, using API5 and FGF2 coordinates, provided a clear electron density map for an API5-FGF2 complex.
( Seoung Hee Kim ),( Min Young Kim ),( Sang Bong Lee ),( In Bea Jang ),( Kyou Seung Lee ) 한국농업기계학회 2013 바이오시스템공학 Vol.38 No.4
Purpose: Using agricultural machinery was not easy in the conventional shading structure, specified as a standard facility by standard cultivation methods for ginseng. Thus, this study designed the new types of facility allowing machine access by modifying the conventional type. Methods: Two types of facility (i.e. wide roof type and long & short roof type) were designed and installed in an experimental site to evaluate its growing environments and applicability of riding-type cultivator. Results: From the results of incoming light measurement, all three types (i.e. two new types and a conventional type) of shading structures blocked the incoming light after 9:00 am. The temperature distribution inside the new types was similar with the one in the conventional type, so the growth of ginseng was in good condition in all three types of facility. The riding-type cultivator was operated well with the low speed first gear of 0.13 m/s in the new types. However, a long & short typed roof needs to be raised 18 cm height in order to use the cultivator. Conclusions: With the results of this study, the new types of roof can be used in the ginseng farm in that they satisfied the growing environments for ginseng and the needs for agricultural mechanization.
Improvement of Shade Structures for Ginseng Cultivation
Kim, Seoung Hee,Kim, Min Young,Lee, Sang Bong,Jang, In Bea,Lee, Kyou Seung Korean Society for Agricultural Machinery 2013 바이오시스템공학 Vol.38 No.4
Purpose: Using agricultural machinery was not easy in the conventional shading structure, specified as a standard facility by standard cultivation methods for ginseng. Thus, this study designed the new types of facility allowing machine access by modifying the conventional type. Methods: Two types of facility (i.e. wide roof type and long & short roof type) were designed and installed in an experimental site to evaluate its growing environments and applicability of riding-type cultivator. Results: From the results of incoming light measurement, all three types (i.e. two new types and a conventional type) of shading structures blocked the incoming light after 9:00 am. The temperature distribution inside the new types was similar with the one in the conventional type, so the growth of ginseng was in good condition in all three types of facility. The riding-type cultivator was operated well with the low speed first gear of 0.13 m/s in the new types. However, a long & short typed roof needs to be raised 18 cm height in order to use the cultivator. Conclusions: With the results of this study, the new types of roof can be used in the ginseng farm in that they satisfied the growing environments for ginseng and the needs for agricultural mechanization.
Lee, Min Sun,Jung, Byung Hwa,Chung, Bong Chul,Cho, Sung Hee,Kim, Ki Young,Kwon, Oh Seoung,Nugraha, Boya,Lee, Young-Joo SAGE Publications 2009 International journal of toxicology Vol.28 No.5
<P> Three different doses of valproic acid (20, 100, and 500 mg/kg/d) are administered orally to Sprague-Dawley rats for 5 days, and the feasibility of metabolomics with gas chromatography-mass spectrometry as a predictor of the hepatotoxicity of valproic acid is evaluated. Body weight is found to decrease with the 100-mg/kg/d dose and significantly decrease with the 500-mg/kg/d dose. Mean excreted urine volume is lowest in the 500-mg/kg/d group among all groups. The plasma level of α-glutathione-S-transferase, a sensitive and earlier biomarker for hepatotoxicity, increases significantly with administration of 100 and 500 mg/kg/d; however, there is not a significant difference in α-glutathione-S-transferase plasma levels between the control and 20-mg/kg/d groups. Clusters in partial least squares discriminant analysis score plots show similar patterns, with changes in physiological conditions and plasma levels of α-glutathione-S-transferase; the cluster for the control and 20-mg/kg/d groups does not clearly separate, but the clusters are separate for 100- and 500-mg/kg/d groups. A biomarker of hepatotoxicity, 8-hydroxy-2′-deoxyguanosine and octanoylcarnitine, is identified from nontargeted and targeted metabolic profiling. These results validate that metabolic profiling using gas chromatography-mass spectrometry could be a useful tool for finding novel biomarkers. Thus, a nontargeted metabolic profiling method is established to evaluate the hepatotoxicity of valproic acid and demonstrates proof-of-concept that metabolomic approach with gas chromatography-mass spectrometry has great potential for predicting valproic acid-induced hepatotoxicity and discovering novel biomarkers. </P>
복막투석 환자에서 간농양에 동반된 Klebsiella pneuoniae 복막염 1예
안승재 ( Seoung Jae An ),김정섭 ( Jung Sub Kim ),손정민 ( Jung Min Son ),성은영 ( Eun Young Seong ),송상헌 ( Sang Heon Song ),이동원 ( Dong Won Lee ),이수봉 ( Soo Bong Lee ),곽임수 ( Ihm Soo Kwak ) 대한신장학회 2010 Kidney Research and Clinical Practice Vol.29 No.1
A 42-year-old male was hospitalized with abdominal pain, dyspnea, and turbid peritoneal fluid. He was diagnosed with hypertension, diabetes and started continuous ambulatory peritoneal dialysis (CAPD) 11 months ago. He was treated with intraperitoneal cefazolin and ceftazidime, and then white blood cell counts of dialysate decreased. Incidentally, liver abscess was found in chest CT performed for the evaluation of dyspnea, and patient was febrile persistently. So percutaneous abscess drainage was done by pigtail catheter. We changed the antibiotics to ceftriaxone and metronidazole, and hemodialysis was started. Klebsiella pneumoniae was cultured from peritoneal fluid and blood simultaneously. We concluded that liver abscess is a primary cause of CAPD peritonitis.
김성민,김동근,김국기,임영진,김태성,이봉암,임언 대한신경외과학회 1991 Journal of Korean neurosurgical society Vol.20 No.8
Multiple intracranial aneurysms occur in range of 15% to 30% of all patients with intracranial aneurysms. The treatment of such patients offers a particular challenge for the neurosurgeon. Early reports dealing with multiple aneurysms recommended treatment of only the ruptured aneurysm, but recently, authors have advised treatment of all significant aneurysms. We performed a retrospective review for 63 patients with multiple intracranial aneurysms, admitted to Department of Neurosurgery, Kyung-Hee University Hospital between Jun. 1982 and Dec. 1989. The present study includes the incidence of multiple aneurysms, its age and sex distribution, aneurysm locations, probability of rupture, relationship of aneurysmal size and rupture, and operative results. The results were as follows ; 1) Multiple intracranial aneurysms occurred in about 12.2% of all patient with aneurysms. 2) The female to male ratio was about 2.3:1 for patients with two aneurysms and 5:1 for patients with three or more aneurysms. 3) Common location for multiple aneurysms were the PCoA, ICA, ACoA, and MCA. 4) On the distribution of multiple aneurysms according to location, unilateral and midline located mutliple aneurysms were 61.9%. 5) Probability of aneurysmal rupture according to site was(in decreasing order) ACoA, PCoA, AChoA, MCA, and ICA bifurcation. 6) Irregularity of shape was more inportant than size in identifying the site of rupture. 7) Most unruptured aneurysms were 5㎜ or smaller in size ; however, most ruptured aneurysms were 6㎜ or larger. 8) About 70% of surgical management showed good and fair result.