렙틴 수용체가 형질 도입된 신경세포에서 ER Stress로 유도된 렙틴저항성에 대한 일산화탄소의 억제작용

김성주,김택상,류현열,정헌택 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

연구 배경 및 목적: 렙틴이 음식의 섭취 양과 체중의 증가를 억제하는 중요한 호르몬 역할을 한다는 사실은 잘 알려졌다. 또한 렙틴에 대한 저항성이 비만의 중요한 원인이라는 연구가 많이 보고되고 있다. 그러나 렙틴 저항성에 대한 구체적인 기전은 아직 밝혀져 있지 않았다. 최근 endoplasmic reticulum (ER) 스트레스에 의한 unfolded protein response (UPR) 가 렙틴에 대한 저항성을 유발한 다는 보고가 있었다. 연구 대상 및 방법: 일산화탄소(carbon monoxide, CO)는 heme oxygenase (HO)-1이 헴(heme)을 산화적 분해로 만들어내는 무색, 무미, 무취의 기체로 항산화, 항염증, 항세포분열 및 항세포고사 작용을 나타내며 조직의 손상에 대한 세포보호작용을 한다. 최근 CO는 ER stress sensor 중의 하나인 protein kinase R-like endoplasmic reticulum kinase (PERK) 를 선택적으로 활성화 시키며 인산화된 PERK 는 HO-1의 전사조절인자인 Nrf2를 인산화로 활성화시켜 결국 HO-1의 유전자 발현을 촉진함이 보고 되였다. 또한 CO로 유도된 PERK의 활성화는 eukaryotic translation initiation factor (eIF) 2a를 인산화로 활성화 시키고 인산화된 eIF2a는 전사인자인 activating transcription factor (ATF)-4의 발현을 유도하지만 CO는 ER stress의 다른 두 개 sensors인 inositol requiring enzyme (IRE) 1a와 하위신호전단물질인 X-box binding protein (xbp)-1이나 ATF6의 활성화에는 영향을 미치지 아니한다. 그러나 ER stress 유발인자들인 thasigargin (TG), tunicamycin (TM), homocysteine 등으로 Xbp-1이나 ATF6가 활성화되는 것을 억제한다. 연구 결과: 본 연구에서는 ER stress 에 의해 활성화되는 UPR에 의해 유발되는 렙틴 저항성이 CO에 의하여 차단될 것이라는 가정을 세우고 다음과 같은 실험결과를 얻었다. TG나 TM에 의해 유도된 ER stress반응 즉 UPR은 렙틴의 신호전달로 야기되는 phospho-signal transducer and activation of transcription (STAT) 3로 인산화되는것을 억제시켰다. 그러나 ER stress를 유도시킬 시 CO를 CO releasing molecule (CORM)으로 처리하였을 때 UPR에 의한 렙틴의 신호전달로 말미암는 STAT3 인산화의 차단이 CO의 양과 노출 시간에 비례하여 제거 되었다. 결론: 이러한 실험결과는 CO에 의한 PERK 인산화는 ER stress 유도체에 의한 IRE1a의 하위 신호전달물질인 Xbp-1 이나 ATF6의 활성화를 억제하여 ER stress 반응인 UPR에 의한 렙틴 저항성을 차단함을 시사한다. 결론적으로 이러한 실험결과는 CO유도체를 이용하여 병인적인 렙틴 저항에 의한 여러 질병을 치료할 수 있음을 시사한다. Background: It is well known that leptin is an important circulating signal for the inhibition of food intake and the gain of body weight. Accumulating data suggest that leptin resistance has been considered to be one of the main causes of obesity. However, the detailed mechanism of leptin resistance is not known yet. Recently, endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR) is reported to be responsible for the leptin resistance. Materials & methods: Carbon monoxide (CO), a reaction product of the cytoprotective heme oxygenase (HO)-1, is anti-apoptotic in a variety of models of cellular injury. Exogenous CO activated Nrf2 through the phosphorylation of protein kinase R-like endoplasmic reticulum kinase (PERK), resulting in HO-1 expression. CO-induced activation of PERK was followed by the phosphorylation of eukaryotic translation initiation factor 2a and the expression of activating transcription factor 4. However, CO fails to induce X-box binding protein-1 expression and activating transcription factor 6 cleavage. CO prevented X-box binding protein-1 expression and activating transcription factor 6 cleavage induced by ER-stress inducer such as thasigargin (TG), tunicamycin (TM) and homocysteine. Results: In the present study, we hypothesized that exogenous CO might block the leptin resistance which is induced by UPR during ER stress. Thasigargin or tunicamycin was used to induce ER stress. The activation status of leptin signals were measured by western blotting analysis using a phospho-signal transducer and activation of transcription3 (STAT3) antibody. In this study, ER stress markedly inhibited leptin-induced STAT3 phosphorylation. Conculusion: These results suggest that ER stress induces leptin resistance and exogenous CO-induced phosphorylation of PERK branch reversed ER stress-induced leptin resistance. Moreover, CO releasing molecule (CORM) blocks the inhibition of leptin-induced STAT3 phosphorylation. Together, the pathological mechanism of leptin resistance could be ameliorated by the use of exogenous CO..

A New Soybean Cultivar for Sprout, "Sojin"

Keum Yong Park,Hong Tae Yun,Jung Kyung Moon,Yeong Ho Lee,Sun Lim Kim,Min Jung Seo,Young Hwan Ryu,Seong Sook Han,Jin Kyo Jung,Seong Ki Kim,Dong Ghan Kim,Ya Seong Lee,Seong Taeg Kim 한국육종학회 2005 한국육종학회지 Vol.37 No.2

Association of changes of pulse wave velocity and augmentation index after isometric handgrip exercise with coronary lesion extent and revascularization

Kim Seong Taeg,Ko Yeekyoung,Beom Jong-Wook,Boo Ki Yung,이재근,Choi Joon-Hyouk,김송이,주승재 대한고혈압학회 2021 Clinical Hypertension Vol.27 No.3

Background: Arterial stiffness is associated with myocardial ischemia and incident coronary artery disease (CAD), and indexes of arterial stiffness are usually increased in patients with CAD. However, these indexes are often increased in elderly without CAD. Arterial stiffness in patients with CAD may become more evident after isometric handgrip exercise which increases systolic pressure and ventricular afterload. We investigated the association of the change of stiffness indexes after isometric handgrip exercise with the lesion extent of CAD and the necessity for coronary revascularization. Methods: Patients who were scheduled a routine coronary angiography via a femoral artery were enrolled. Arterial waveforms were traced at aortic root and external iliac artery using coronary catheters at baseline and 3 min after handgrip exercise. Augmentation index (AIx) was measured on the recorded aortic pressure waveform, and pulse wave velocity (PWV) was calculated using the ECG-gated time difference of the upstroke of arterial waveforms and distance between aortic root and external iliac artery. Results: Total 37 patients were evaluated. Both PWV and AIx increased after handgrip exercise. ΔPWV was significantly correlated with ΔAIx (r = 0.344, P = 0.037). Patients were divided into higher and lower ΔPWV or ΔAIx groups based on the median values of 0.4 m/sec and 3.3%, respectively. Patients with higher PWV had more 2- or 3-vessel CAD (69% vs. 27%, P = 0.034), and underwent percutaneous coronary intervention (PCI) more frequently (84% vs. 50%, P = 0.038), but higher ΔAIx was not associated with either the lesion extent or PCI. Area under curve (AUC) of ΔPWV in association with PCI by C-statistics was 0.70 (95% confidence interval [CI] 0.51–0.88; P = 0.056). In multiple logistic regression analysis, ΔPWV was significantly associated with PCI (odds ratio 7.78; 95% CI 1.26–48.02; P = 0.027). Conclusions: Higher ΔPWV after isometric handgrip exercise was associated with the lesion extent of CAD and the necessity for coronary revascularization, but higher ΔAIx was not.

A Study on the Short-Term Plan of Structural Reinforcing and the Temporary Housing Supply Strategy through the Analysis of the Status of North Korean Standard Housing

Seong Eun Kim,Seung Hun Kim,Yong Taeg Lee 대한건축학회 2018 Architectural research Vol.20 No.3

Recently, there has been inter-Korean exchanges are active, in company with an assertive international activity with North Korea and inter-Korean Summits. Thus, there is a growing possibility of Korean reunification. However, when the unification situation occurs, population inflow by North Korean residents will be expected, and it will be necessary to prepare a housing supply strategy. In addition, even if North Koreans are prevented from entering, a plan is necessary to ensure structural safety since most buildings in North Korea are aging and a recent natural disaster occurs frequently. In this research, we will try to present a temporary housing supply strategy and a short-term structural reinforcement plan based on analysis of the standard housing conditions in North Korea. In this research, we represent the analysis result of the existing construction level, supply status and structural performance of North Korea. When we consider the reinforcement period and social costs, a short-term reinforcement plan is recommended rather than the full reinforcement of the aged housing in North Korea. Furthermore, we examine the ways to secure the residential safety of North Koreans through a case study of domestic and overseas temporary residential facilities, until the permanent residential facilities were supplied. According to the analysis, the tent houses were used as a form of relief housing in overseas frequently. However, the tent house is difficult to apply for domestic country with clear seasons due to insulation and heating problems. Therefore, it is appropriate to develop a residential supply strategy as focusing on the temporary housing facilities in assembled or container from, in preparation for the future population inflow.

The Korean Association of lnternal Medicine : Poster Session ; PS 1333 : Nephrology ; Occult Hepatitis B Virus Infection of Haemodialysis Patients: A Cross-Sectional Study in a Hepatitis B Virus- Endemic Region

( Seong Taeg Kim ),( So Mi Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Occult HBV infection of haemodialysis (HD) patients is informative in terms of virus transmission, reactivation after kidney transplantation, and the progression of liver disease. However, there is little detailed information about occult HBV infection in the context of virus endemicity. We tried to investigate the seroprevalence and clinical features of occult HBV infection in HD patients in HBV-endemic regions. Methods: We enrolled a total of 159 HD patients and 121 apparently healthy subjects at Dankook University Hospital and Jeju National University Hospital in Korea. HBsAg, anti-HBs, anti-HBc, and anti-hepatitis C virus (HCV) antibody levels were measured by radioimmunoassay. Serum levels of HBV DNA were measured by real-time polymerase chain reaction. Results: The seroprevalence of occult HBV infection was 1.3% in HD patients and 2.5% in the healthy controls. This difference was not signifi cant. The HBV load in all subjects with occult infection was <116 copies/mL, and all were positive for IgG anti-HBc, regardless of the presence of anti-HBs. None of the occult HBV-infected subjects were co-infected with HCV. One of the two HD patients with occult HBV infection had no history of blood transfusion. Conclusions: In this HBV-endemic region, the seroprevalence of occult HBV infection in HD patients with a very low viral load was not signifi cantly different from that in apparently healthy subjects.

패혈중환자에서 혈청 TNF-α 및 IL-1β 농도 측정의 임상적 의의

김재열 ( Kim Jae Yeol ),이춘택 ( Lee Chun Taeg ),김영환 ( Kim Yeong Hwan ),한성구 ( Han Seong Gu ),민경업 ( Min Gyeong Eob ),김유영 ( Kim Yu Yeong ),심영수 ( Sim Yeong Su ),유철규 ( Yu Cheol Gyu ) 대한결핵 및 호흡기학회 2000 Tuberculosis and Respiratory Diseases Vol.49 No.2

북한 주택현황 및 건축구조형식 분석

김성은 ( Kim Seong Eun ),정재원 ( Jeong Jae Won ),김영인 ( Kim Young In ),김승훈 ( Kim Seung Hun ),이용택 ( Lee Yong Taeg ) 한국구조물진단유지관리공학회 2018 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.22 No.1

This study investigated and analyzed the housing type of North Korea and the current status of standard housing construction. The purpose of this study is to establish a strategy for securing the safety of North Korean standard houses with relatively low structural performance compared to South Korea. In addition, we will use this data as basic data for planning a low-cost emergency reinforcement method to prepare against the natural disasters that are taking place frequently.

유미흉으로 발현된 말초성 T세포 림프종 1예

김성택 ( Seong Taeg Kim ),조재민 ( Jaemin Jo ),유정래 ( Jeong Rae Yoo ),김미연 ( Miyeon Kim ),한경희 ( Kyoung Hee Han ),김정호 ( Jung Ho Kim ),한상훈 ( Sang Hoon Han ) 대한내과학회 2017 대한내과학회지 Vol.92 No.1

말초형 T세포 림프종은, 림프절외 증상이 비특이적으로 나타날 경우 질병이 진행하기 전까지 진단이 어려울 수 있다. 본 증례는 T세포 림프종에 의한 림프절 종대의 빠른 진행과 함께 드문 합병증인 유미흉으로 발현한 환자이며 비특정 말초 T세포 림프종으로 확진된 1예로 호흡곤란을 유발하는 유미흉을 적절히 조절하여, 항암화학요법을 속히 시행하고 부분반응을 얻을 수 있었던 증례이다. A 72-year-old male presented with respiratory discomfort. A simple chest X-ray and abdominal computed tomography showed pleural effusion and multiple lymph node enlargement. The pleural effusion was determined by thoracentesis to be chylothorax. An inguinal lymph node biopsy showed peripheral T-cell lymphoma. Following three cycles of cyclophospamide, hydroxyl doxorubicin, vincristine, prednisolone (CHOP) chemotherapy, a partial response was observed. Chylothorax is an extremely rare complication of T-cell lymphoma. We present a case of peripheral T-cell lymphoma presenting with chylothorax. We suggest that clinicians should consider chylothorax when examining patients with lymphoma who present with atypical pleural effusion. (Korean J Med 2017;92:74-78)

대퇴골두 무혈성괴사증 환자에 있어서 Harris-Galante 삽입물을 이용한 고관절 전치환술

김성중 ( Kim Seong Jung ),김신윤 ( Kim Sin Yun ),김용구 ( Kim Yong Gu ),박일형 ( Park Il Hyeong ),박병철 ( Park Byeong Cheol ),김풍택 ( Kim Pung Taeg ) 대한고관절학회 2003 Hip and Pelvis Vol.15 No.1

박판 탄소강 이면전단 볼트접합부의 종국거동에 관한 실험적 연구

김태수(Kim Tae-Soo),김민성(Kim Min-Seong),정하영(Jeong Ha-Young),김승훈(Kim Seung-Hun),이용택(Lee Yong-Taeg) 대한건축학회 2009 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.29 No.1(구조계)

Experimental studies of thin-walled carbon steel single shear bolted connections with end distance and plate thickness as main variables have been performed by Kim et al. and block shear fracture behavior and the influence of curling on ultimate strength have been investigated. As a result, bolted connections with short end distance and thick plate thickness failed by typical block shear fracture, whereas, curling occurred in connections with long end distance and thin plate thickness and resulted in the strength reduction. In this study, thin-walled carbon steel double shear bolted connections with same dimension of existing single shear bolted connections are planned and tested in order to investigate the fracture mode and ultimate strength. Since curling was restrained by outside thick plate, out of deformation was not observed in double shear specimens. Moreover, ultimate strengths of the test results are compared with those of single shear bolted connections.