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Induction of growth arrest by miR-542-3p that targets survivin
Yoon, Sena,Choi, Young-Chul,Lee, Suman,Jeong, Yongsu,Yoon, Jaeseung,Baek, Kwanghee Elsevier 2010 FEBS letters Vol.584 No.18
<P><B>Abstract</B></P><P>Survivin is a protein which functions as a mitotic regulator as well as apoptosis inhibitor. In this study, we show that introduction of synthetic miR-542-3p mimetic reduced both mRNA and protein levels of survivin. In A549 cells, luciferase reporter assay revealed that miR-542-3p targeted predicted binding sites in the 3′-untranslated region (3′-UTR) of survivin. We also demonstrate that ectopic expression of miR-542-3p inhibited cell proliferation by inducing Gap 1 (G1) and Gap 2/Mitosis (G2/M) cell cycle arrest. Collectively, these results suggest that survivin is a direct target of miR-542-3p and growth inhibition by miR-542-3p may have a potential utility as an anti-cancer therapy.</P>
Inhibition of Cell Proliferation and Migration by miR-509-3p That Targets CDK2, Rac1, and PIK3C2A
Yoon, Sena,Han, Eunji,Choi, Young-Chul,Kee, Honghwan,Jeong, Yongsu,Yoon, Jaeseung,Baek, Kwanghee Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.4
CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3'-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.
( Juyoung Byun ),( Sena Yoon ),( Yunji Jeong ),( Uitaek Oh ),( Sujin Cho ),( Jeongsoo Park ),( Yongsu Jeong ),( Kwanghee Baek ),( Jaeseung Yoon ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.1
Development of stable rCHO cell lines is still time consuming and labor intensive, although it is a critical step in the commercial development of recombinant antibodies. The current work demonstrates, for the first time, that electroporation of CHO cells with DMSO can enhance stable expression of recombinant antibodies in rCHO cells. Electroporation with DMSO resulted in an average 3.7-fold and 2.8-fold increases in expression levels of aflibercept and pembrolizumab, respectively, in pools of stable rCHO cells. It also resulted in an average of 2.2-fold and 2.6-fold increases in the expression of aflibercept and pembrolizumab, respectively, in single-cell derived rCHO clones. Simple batch cultures of rCHO cell clones with the highest expression produced 1.0 g/l for aflibercept and 1.4 g/l for pembrolizumab without a time-consuming gene amplification process. Electroporation with DMSO also shortened the development of rCHO cell lines to 2-3 months, allowing rapid establishment of stable rCHO cell lines with a desirable expression level antibodies.
SELECT 모델을 이용한 트롤 비교 시험조업법에 의한 망목 선택성에 관한 연구
김성훈,김형석,백세나,김재형,김병관,Seonghun KIM,Hyungseok KIM,Sena BAEK,Jaehyung KIM,Pyungkwan KIM 한국수산해양기술학회 2023 수산해양기술연구 Vol.59 No.2
In this study, a comparative test operation was conducted through the alternate haul method to examine the selectivity of the four mesh sizes (60 mm, 90 mm, 110 mm, and 130 mm) of the trawl codend. The selectivity was analyzed using the SELECT model considering the fishing efficiency (split parameter) of each fishing gear in the comparative test fishing operation in the trawl and the maximum likelihood method for parameter estimation. A selectivity master curve was estimated for several mesh sizes using the extended-SELECT model. As a result of analyzing the selectivity for silver croaker based on the results of three times hauls for each experimental gear, it was found that the size of the fish caught increased as the size of the mesh size increased. When the selectivity for each mesh size analyzed by the SELECT model considering the split ratio was evaluated based on the size of the AIC value, the estimated split model was superior to the equal split model. Based on the master curve, the 50% selection length value was 2.893, which was estimated to be 136 mm based on the mesh size of 60 mm. In some selectivity models, there was a large deviance between observed and theoretical values due to the non-uniformity of the distribution of fished length classes. As a result, it is considered that appropriate sea trials and selectivity evaluation methods with high reliability should be applied to present trawl fishery resource management methods.